kshA Protein, Mycobacterium tuberculosis (His, Strep)
kshA Protein, Mycobacterium tuberculosis (His, Strep) is the recombinant kshA, expressed by E. coli , with Strep, His labeled tag. ,
- Species: Others
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
kshA Protein, Mycobacterium tuberculosis (His, Strep) is the recombinant kshA, expressed by E. coli , with Strep, His labeled tag. ,
Background
kshA is an enzyme involved in cholesterol degradation, catalyzing the introduction of a 9a-hydroxyl moiety into 1,4-androstadiene-3,17-dione (ADD) to produce the 9alpha-hydroxy-1,4-androstadiene-3,17-dione (9OHADD) intermediate, which spontaneously forms 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione (HSA) via meta-cleavage of ring B and aromatization of ring A. Additionally, KSH can utilize 4-androstene-3,17-dione (AD), 3-oxo-23,24-bisnorcholesta-4-en-22-oate (4-BNC), 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate (1,4-BNC), 3-oxo-23,24-bisnorcholesta-4-en-22-oyl-coenzyme A thioester (4-BNC-CoA), and 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-coenzyme A thioester (1,4-BNC-CoA) as substrates.
Technical Parameters
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Species Others
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Source E. coli
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Tag Strep;His
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Accession
P71875 (M1-H386)
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Gene ID888268
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Protein Length
Full Length
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Synonyms
3-ketosteroid-9-alpha-monooxygenase, oxygenase component; 3-ketosteroid-9-alpha-hydroxylase, oxygenase component; KSH; Androsta-1,4-diene-3,17-dione 9-alpha-hydroxylase; EC:1.14.15.30; Rieske-type oxygenase; RO; kshA; Rv3526
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AA Sequence
MSTDTSGVGVREIDAGALPTRYARGWHCLGVAKDYLEGKPHGVEAFGTKLVVFADSHGDLKVLDGYCRHMGGDLSEGTVKGDEVACPFHDWRWGGDGRCKLVPYARRTPRMARTRSWTTDVRSGLLFVWHDHEGNPPDPAVRIPEIPEAASDEWTDWRWNRILIEGSNCRDIIDNVTDMAHFFYIHFGLPTYFKNVFEGHIASQYLHNVGRPDVDDLGTSYGEAHLDSEASYFGPSFMINWLHNRYGNYKSESILINCHYPVTQNSFVLQWGVIVEKPKGMSEEMTDKLSRVFTEGVSKGFLQDVEIWKHKTRIDNPLLVEEDGAVYQLRRWYEQFYVDVADIKPEMVERFEIEVDTKRANEFWNAEVEKNLKSREVSDDVPAEQH
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Predicted Molecular Mass
47.5 kDa
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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