2. Epigenetics TGF-beta/Smad Metabolic Enzyme/Protease
  3. PKC Phosphatase Endogenous Metabolite
  4. D-erythro-Sphingosine

D-erythro-Sphingosine  (Synonyms: Erythrosphingosine; erythro-C18-Sphingosine; trans-4-Sphingenine; D-erythro-スフィンゴシン; C18-スフィンゴシン)

製品番号: HY-101047 純度: 98.0%
COA 取扱説明書 Technical Support

D-erythro-Sphingosine (Erythrosphingosine) is a very potent activator of p32-kinase with an EC50 of 8 μM, and inhibits protein kinase C (PKC). D-erythro-Sphingosine (Erythrosphingosine) is also a PP2A activator.

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CAS 番号 : 123-78-4

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10 mM * 1 mL in DMSO
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Solution
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Solid
5 mg $32 在庫あり
10 mg $50 在庫あり
25 mg $80 在庫あり
50 mg $140 在庫あり
100 mg $210 在庫あり
500 mg $525 在庫あり
1 g $840 在庫あり
5 g $2100 在庫あり
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カスタマーレビュー

Based on 4 publication(s) in Google Scholar

Other Forms of D-erythro-Sphingosine:

D-erythro-Sphingosine 関連抗体

Top Publications Citing Use of Products

    D-erythro-Sphingosine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Sep 19;15(1):8221.  [Abstract]

    D-erythro-Sphingosine (10 μM, 30 min). Effects of sph addition in different ways in ZIKV-infected cells. The protein levels were quantified using western blot at 48 h post infection. Con.: DMSO treatment.

    D-erythro-Sphingosine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Sep 19;15(1):8221.  [Abstract]

    D-erythro-Sphingosine (10 μM, 30 min). Effects of sph addition in different ways in ZIKV-infected cells. ZIKV mRNA levels were examined using qPCR (b; n = 3 biologically independent cell samples; two-sided multiple unpaired t-test) at 48 h post infection. Con.: DMSO treatment.

    D-erythro-Sphingosine purchased from MedChemExpress. Usage Cited in: Mol Med. 2022 Sep 6;28(1):106.  [Abstract]

    Primary cells were treated with solvent, 5 μM of sphingosine, 10 μM of DASA58 plus sphingosine, and 200 nM of cerulein plus 10 ng/mL of LPS and DASA58 with LPS and sphingosine for 6 h. Cells were lysed for trypsin assay.

    D-erythro-Sphingosine purchased from MedChemExpress. Usage Cited in: Mol Med. 2022 Sep 6;28(1):106.  [Abstract]

    Primary cells were treated with solvent, 5 μM of sphingosine, 10 μM of DASA58 plus sphingosine, and 200 nM of cerulein plus 10 ng/mL of LPS and DASA58 with LPS and sphingosine for 6 h. Cells were lysed for cathepsin B assay by WB.

    D-erythro-Sphingosine purchased from MedChemExpress. Usage Cited in: Mol Med. 2022 Sep 6;28(1):106.  [Abstract]

    Acid ceramidase (ASAH1) regulated the activity of trypsin and cathepsin B through sphingosine (SPH). Lentivirus particles containing nontargeting shRNA/Control vector or shRNA targeting ASAH1/ASAH1 expressing plasmid were added to primary pancreatic acinar cells. Additionally, the cells were treated as indicated. Cathepsin B activity was detected by a spectrophotometer.

    PKC アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

    • 生物活性

    • 純度とドキュメンテーション

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    • カスタマーレビュー

    製品説明

    D-erythro-Sphingosine (Erythrosphingosine) is a very potent activator of p32-kinase with an EC50 of 8 μM, and inhibits protein kinase C (PKC). D-erythro-Sphingosine (Erythrosphingosine) is also a PP2A activator[1][2][3][4].

    IC50 & Target[1][2][3][4]

    p32

    8 μM (EC50)

    PKC

     

    PP2A

     

    Human Endogenous Metabolite

     

    Microbial Metabolite

     

    Cellular Effect
    Cell Line Type Value Description References
    FL5.12 IC50
    3.6 μM
    Compound: 6
    Cytotoxicity against mouse FL5.12A cells after 48 hrs by DAPI staining-based flow cytometric analysis
    Cytotoxicity against mouse FL5.12A cells after 48 hrs by DAPI staining-based flow cytometric analysis
    		[PMID: 27475534]
    Jurkat IC50
    2.01 μM
    Compound: Sphingosine
    Inhibition of wild type human Jurkat cells after 18 hrs by MTT assay
    Inhibition of wild type human Jurkat cells after 18 hrs by MTT assay
    		[PMID: 17400555]
    Jurkat IC50
    2.88 μM
    Compound: Sphingosine
    Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR2 mutant cells proliferation after 18 hrs by MTT assay
    Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR2 mutant cells proliferation after 18 hrs by MTT assay
    		[PMID: 17400555]
    Jurkat IC50
    3.38 μM
    Compound: Sphingosine
    Inhibition of human 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR3 mutant cells proliferation after 18 hrs by MTT assay
    Inhibition of human 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR3 mutant cells proliferation after 18 hrs by MTT assay
    		[PMID: 17400555]
    Jurkat IC50
    3.48 μM
    Compound: Sphingosine
    Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR1 mutant cells proliferation after 18 hrs by MTT assay
    Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR1 mutant cells proliferation after 18 hrs by MTT assay
    		[PMID: 17400555]
    体外実験

    A p32-sphingosine-activated protein kinase responds to low concentrations of D-erythro-Sphingosine with an initial activation observed at 2.5 μM and a peak activity at 10-20 μM. This kinase shows a modest specificity for D-erythro-Sphingosine over other sphingosine tereoisomers, and a preference for sphingosines over ihydrosphingosines[1]. D-erythro-Sphingosine inhibits protein kinase C in vitro[2]. D-erythro-Sphingosine has been shown to inhibit protein kinase C, which affects cell regulation and several signal transduction pathways, and exhibits antitumor promoter activities in various mammalian cells[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    D-erythro-Sphingosine 関連抗体
    分子量

    299.49

    分子式

    C18H37NO2
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CCCCCCCCCCCCC/C=C/[C@@H](O)[C@@H](N)CO
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 50 mg/mL (166.95 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.3390 mL 16.6950 mL 33.3901 mL
    5 mM 0.6678 mL 3.3390 mL 6.6780 mL
    10 mM 0.3339 mL 1.6695 mL 3.3390 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (8.35 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (8.35 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 98.0%

    COA (239 KB) HNMR (247 KB) MS (204 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.3390 mL 16.6950 mL 33.3901 mL 83.4752 mL
    5 mM 0.6678 mL 3.3390 mL 6.6780 mL 16.6950 mL
    10 mM 0.3339 mL 1.6695 mL 3.3390 mL 8.3475 mL
    15 mM 0.2226 mL 1.1130 mL 2.2260 mL 5.5650 mL
    20 mM 0.1670 mL 0.8348 mL 1.6695 mL 4.1738 mL
    25 mM 0.1336 mL 0.6678 mL 1.3356 mL 3.3390 mL
    30 mM 0.1113 mL 0.5565 mL 1.1130 mL 2.7825 mL
    40 mM 0.0835 mL 0.4174 mL 0.8348 mL 2.0869 mL
    50 mM 0.0668 mL 0.3339 mL 0.6678 mL 1.6695 mL
    60 mM 0.0557 mL 0.2783 mL 0.5565 mL 1.3913 mL
    80 mM 0.0417 mL 0.2087 mL 0.4174 mL 1.0434 mL
    100 mM 0.0334 mL 0.1670 mL 0.3339 mL 0.8348 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    D-erythro-Sphingosine Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    D-erythro-Sphingosine123-78-4Erythrosphingosine erythro-C18-Sphingosine trans-4-SphingeninePKCPhosphataseEndogenous MetaboliteProtein kinase CInhibitorinhibitorinhibit

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    製品名:
    D-erythro-Sphingosine
    製品番号:
    HY-101047
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