D-erythro-Sphingosine hydrochloride (Synonyms: Erythrosphingosine hydrochloride; erythro-C18-Sphingosine hydrochloride; trans-4-Sphingenine hydrochloride)

Name: D-erythro-Sphingosine hydrochloride
Brand: MedChemExpress
SKU: HY-W089922
Rating: 5.0 (4 reviews)

Cat. No.: HY-W089922 Purity: 99.95%: Data Sheet
COA

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D-erythro-Sphingosine (Erythrosphingosine) hydrochloride is a specific TRPM3 activator. D-erythro-Sphingosine also induces retinoblastoma protein dephosphorylation.

For research use only. We do not sell to patients.

CAS No. : 2673-72-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of D-erythro-Sphingosine hydrochloride:

D-erythro-Sphingosine In-stock

4 Publications Citing Use of MCE D-erythro-Sphingosine hydrochloride

Bio/Physico-chemical Assay

RT-PCR

: D-erythro-Sphingosine hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Sep 19;15(1):8221. [Abstract]; D-erythro-Sphingosine (10 μM, 30 min). Effects of sph addition in different ways in ZIKV-infected cells. The protein levels were quantified using western blot at 48 h post infection. Con.: DMSO treatment.

: D-erythro-Sphingosine hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Sep 19;15(1):8221. [Abstract]; D-erythro-Sphingosine (10 μM, 30 min). Effects of sph addition in different ways in ZIKV-infected cells. ZIKV mRNA levels were examined using qPCR (b; n = 3 biologically independent cell samples; two-sided multiple unpaired t-test) at 48 h post infection. Con.: DMSO treatment.

: D-erythro-Sphingosine hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Med. 2022 Sep 6;28(1):106. [Abstract]; Primary cells were treated with solvent, 5 μM of sphingosine, 10 μM of DASA58 plus sphingosine, and 200 nM of cerulein plus 10 ng/mL of LPS and DASA58 with LPS and sphingosine for 6 h. Cells were lysed for trypsin assay.

: D-erythro-Sphingosine hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Med. 2022 Sep 6;28(1):106. [Abstract]; Primary cells were treated with solvent, 5 μM of sphingosine, 10 μM of DASA58 plus sphingosine, and 200 nM of cerulein plus 10 ng/mL of LPS and DASA58 with LPS and sphingosine for 6 h. Cells were lysed for cathepsin B assay by WB.

: D-erythro-Sphingosine hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Med. 2022 Sep 6;28(1):106. [Abstract]; Acid ceramidase (ASAH1) regulated the activity of trypsin and cathepsin B through sphingosine (SPH). Lentivirus particles containing nontargeting shRNA/Control vector or shRNA targeting ASAH1/ASAH1 expressing plasmid were added to primary pancreatic acinar cells. Additionally, the cells were treated as indicated. Cathepsin B activity was detected by a spectrophotometer.

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View All TRP Channel Isoform Specific Products:

View All Isoforms

TRPA1 TRPC3 TRPC4 TRPC5 TRPC6 TRPC7 TRPM8 TRPM2 TRPM3 TRPM4 TRPM7 TRPML1 TRPML2 TRPML3 TRPV1 TRPV2 TRPV3 TRPV4 TRPV6

Biological Activity
Purity & Documentation
References
Customer Review

Description

D-erythro-Sphingosine (Erythrosphingosine) hydrochloride is a specific TRPM3 activator. D-erythro-Sphingosine also induces retinoblastoma protein dephosphorylation^[1]^[2].

IC₅₀ & Target^[1]^[2]

TRPM3

Retinoblastoma protein

In Vitro

Extracellular application of D-erythro-Sphingosine (20 μM) induces an increase in [Ca²⁺]_i in TRPM3-transfected HEK293 cells within 20 to 30 s after start of application, whereas nontransfected control cells (NT) shows only very small responses^[1].
D-erythro-Sphingosine (10 μM) induces currents through TRPM3^[1].
Induction of retinoblastoma protein dephosphorylation by D-erythro-Sphingosine (500 nM; 24 h) precede inhibition of growth and a specific arrest in the G0/G1 phase of the cell cycle^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

335.95

Formula

C₁₈H₃₈ClNO₂

CAS No.

2673-72-5

Appearance

Solid

Color

White to off-white

SMILES

CCCCCCCCCCCCC/C=C/[C@@H](O)[C@@H](N)CO.[H]Cl

Initial Source

Animals

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (297.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 50 mg/mL (148.83 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9766 mL	14.8832 mL	29.7663 mL
5 mM	0.5953 mL	2.9766 mL	5.9533 mL
10 mM	0.2977 mL	1.4883 mL	2.9766 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (269 KB) SDS (394 KB)

COA (240 KB)

Handling Instructions (2659 KB)

References

[1]. Grimm C, et al. Activation of the melastatin-related cation channel TRPM3 by D-erythro-sphingosine [corrected]. Mol Pharmacol. 2005 Mar;67(3):798-805. [Content Brief]

[2]. Chao R, et al. Retinoblastoma protein dephosphorylation induced by D-erythro-sphingosine. J Biol Chem. 1992 Nov 25;267(33):23459-62. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	2.9766 mL	14.8832 mL	29.7663 mL	74.4158 mL
	5 mM	0.5953 mL	2.9766 mL	5.9533 mL	14.8832 mL
	10 mM	0.2977 mL	1.4883 mL	2.9766 mL	7.4416 mL
	15 mM	0.1984 mL	0.9922 mL	1.9844 mL	4.9611 mL
	20 mM	0.1488 mL	0.7442 mL	1.4883 mL	3.7208 mL
	25 mM	0.1191 mL	0.5953 mL	1.1907 mL	2.9766 mL
	30 mM	0.0992 mL	0.4961 mL	0.9922 mL	2.4805 mL
	40 mM	0.0744 mL	0.3721 mL	0.7442 mL	1.8604 mL
	50 mM	0.0595 mL	0.2977 mL	0.5953 mL	1.4883 mL
	60 mM	0.0496 mL	0.2481 mL	0.4961 mL	1.2403 mL
	80 mM	0.0372 mL	0.1860 mL	0.3721 mL	0.9302 mL
	100 mM	0.0298 mL	0.1488 mL	0.2977 mL	0.7442 mL

D-erythro-Sphingosine hydrochloride Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

D-erythro-Sphingosine2673-72-5Erythrosphingosineerythro-C18-Sphingosinetrans-4-SphingenineTRP ChannelTransient receptor potential channelsretinoblastoma proteinHEK293Inhibitorinhibitorinhibit

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D-erythro-Sphingosine hydrochloride (Synonyms: Erythrosphingosine hydrochloride; erythro-C18-Sphingosine hydrochloride; trans-4-Sphingenine hydrochloride)

Customer Review

Other Forms of D-erythro-Sphingosine hydrochloride:

D-erythro-Sphingosine hydrochloride Related Antibodies

4 Publications Citing Use of MCE D-erythro-Sphingosine hydrochloride

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All TRP Channel Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

D-erythro-Sphingosine hydrochloride Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

D-erythro-Sphingosine hydrochloride Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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