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  3. L-Ascorbic acid

アスコルビン酸  (Synonyms: Vitamin C; L-Ascorbic acid; Vitamin C; 50-81-7)

製品番号: HY-B0166 純度: 99.96%
COA 取扱説明書 Technical Support

L-Ascorbic acid (L-Ascorbate), an electron donor, is an endogenous antioxidant agent. L-Ascorbic acid inhibits selectively Cav3.2 channels with an IC50 of 6.5 μM. L-Ascorbic acid is also a collagen deposition enhancer and an elastogenesis inhibitor. L-Ascorbic acid exhibits anti-cancer effects through the generation of reactive oxygen species (ROS) and selective damage to cancer cells.

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CAS 番号 : 50-81-7

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10 mM * 1 mL in DMSO
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カスタマーレビュー

Based on 80 publication(s) in Google Scholar

Other Forms of L-Ascorbic acid:

Top Publications Citing Use of Products

顧客検証

2D/3D Cell Culture and Differentiation
Cell Proliferation/Viability Assay
WB
IHC

    L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Biomaterials. 2024 Aug 15:313:122756.  [Abstract]

    (A) Representative images of Alizarin Red staining on 14 and 21 days of BMSCs seeded on porous tantalum scaffolds and cultured in osteogenic induction medium containing 1 M β-glycerophosphate (β-Glycerophosphate disodium salt pentahydrate) and 10 mM ascorbic acid ( L-Ascorbic acid). (B) Quantitative analysis of dissolved calcium nodules after 14 days and 21 days (n = 4).

    L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2024 May 27;13(6):653.

    L-Ascorbic acid (Vitamin C) (50-500 μM; 6 h) significantly increases the viability of heat-stressed gastric cancer cells at concentrations of 200 μM and 300 μM.

    L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Cancer Cell Int. 2024 May 31;24(1):192.  [Abstract]

    L-Ascorbic acid (AA) (150 μL of 1.5 M; i.p.; every two days) enhances the expression of GPX4 and SLC7A11 proteins in mouse liver.

    L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Cancer Cell Int. 2024 May 31;24(1):192.  [Abstract]

    L-Ascorbic acid (AA) (150ul of 1.5 M; i.p.; every two days) significantly decreases the expression of Ki67 in C57BL/6 mice.

    L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Food Funct. 2022 May 10;13(9):5089-5101.  [Abstract]

    L-Ascorbic acid (Vitamin C) (100 μM; 10 min-4 h) upregulates the protein expression levels of IGF-1, p-IGF-1R, and SVCT2 in a time-dependent manner.

    L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2023 Jun;39(3):679-702.  [Abstract]

    L-Ascorbic acid (300 μM, 10 μL intrathecal injection) reduces the levels of CaV3.2, p-CaMKII, and Cx43 in mice.

    L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Antioxid Redox Signal. 2020 Dec 10;33(17):1191-1208.  [Abstract]

    L-Ascorbic acid (Vitamin C) (250 μM, 1 h) significantly alleviates the growth suppression induced by combined SAS and PAG treatment only in OVISE cells, but shows no effect in KOC-7C cells.
    • 生物活性

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    • 参考文献

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    製品説明

    L-Ascorbic acid (L-Ascorbate), an electron donor, is an endogenous antioxidant agent. L-Ascorbic acid inhibits selectively Cav3.2 channels with an IC50 of 6.5 μM. L-Ascorbic acid is also a collagen deposition enhancer and an elastogenesis inhibitor[1][2][3]. L-Ascorbic acid exhibits anti-cancer effects through the generation of reactive oxygen species (ROS) and selective damage to cancer cells[4].

    IC50 & Target

    T-type calcium channel

     

    Microbial Metabolite

     

    Cellular Effect
    Cell Line Type Value Description References
    A549 IC50
    >100 μM
    Compound: L-ASA
    Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    [PMID: 31586832]
    CFPAC-1 IC50
    >100 μM
    Compound: L-ASA
    Antiproliferative activity against human CFPAC-1 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    Antiproliferative activity against human CFPAC-1 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    [PMID: 31586832]
    Erythrocyte EC50
    119.87 μg/mL
    Compound: Ascorbic acid
    Antioxidant activity in erythrocytes assessed as inhibition of hemolysis
    Antioxidant activity in erythrocytes assessed as inhibition of hemolysis
    [PMID: 22677320]
    Erythrocyte EC50
    96.625 μg/mL
    Compound: Ascorbic acid
    Hemolysis of human erythrocytes after 30 mins by spectrophotometry
    Hemolysis of human erythrocytes after 30 mins by spectrophotometry
    [PMID: 23811092]
    HCT-116 IC50
    >100 μM
    Compound: L-ASA
    Antiproliferative activity against human HCT116cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    Antiproliferative activity against human HCT116cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    [PMID: 31586832]
    HEK293 EC50
    0.5 nM
    Compound: Ascorbic acid
    Agonist activity at human adrenergic alpha2B receptor expressed in HEK293 cells coexpressing Gqi5 protein by FLIPR assay
    Agonist activity at human adrenergic alpha2B receptor expressed in HEK293 cells coexpressing Gqi5 protein by FLIPR assay
    [PMID: 19243956]
    HEK293 EC50
    156 μM
    Compound: 54670067
    Substrate uptake and inhibition of the Na(+)/L-Ascorbic Acid Transporter 1 (SVCT1, SLC23A1) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC23A1 cells (PubChem AID: 1794810)
    Substrate uptake and inhibition of the Na(+)/L-Ascorbic Acid Transporter 1 (SVCT1, SLC23A1) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC23A1 cells (PubChem AID: 1794810)
    10.5281/zenodo.7360349
    HeLa IC50
    >100 μM
    Compound: L-ASA
    Antiproliferative activity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    Antiproliferative activity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    [PMID: 31586832]
    HepG2 IC50
    >100 μM
    Compound: L-ASA
    Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    [PMID: 31586832]
    HFF IC50
    >100 μM
    Compound: L-ASA
    Cytotoxicity against human HFF cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    Cytotoxicity against human HFF cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    [PMID: 31586832]
    HL-60 IC50
    0.01 μM
    Compound: Vitamin C
    Antioxidant activity in human HL60 cells assessed as inhibition of relative oxygen species generation by DCFH-DA method
    Antioxidant activity in human HL60 cells assessed as inhibition of relative oxygen species generation by DCFH-DA method
    [PMID: 19555121]
    HL-60 IC50
    10.2 μM
    Compound: Vitamin C
    Antioxidant activity in human HL60 cells assessed as inhibition of TPA-stimulated hydrogen peroxide induced DCFH-DA oxidation by fluorometric microplate assay
    Antioxidant activity in human HL60 cells assessed as inhibition of TPA-stimulated hydrogen peroxide induced DCFH-DA oxidation by fluorometric microplate assay
    [PMID: 16562834]
    HL-60 IC50
    9.7 μM
    Compound: Vitamin C
    Antioxidant activity against TPA-induced ROS production in human HL60 cells by 2',7'-dichlorodihydrofluorescein diacetate cellular-based assay
    Antioxidant activity against TPA-induced ROS production in human HL60 cells by 2',7'-dichlorodihydrofluorescein diacetate cellular-based assay
    [PMID: 12762791]
    J774.A1 IC50
    >100 μM
    Compound: AA
    Antiinflammatory activity in mouse J774.A1 cells assessed as inhibition of LPS-induced NO production incubated for 24 hrs by Griess reagent based analysis
    Antiinflammatory activity in mouse J774.A1 cells assessed as inhibition of LPS-induced NO production incubated for 24 hrs by Griess reagent based analysis
    [PMID: 33422907]
    MCF7 IC50
    >100 μM
    Compound: L-ASA
    Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    [PMID: 31586832]
    RD EC50
    >2270 μM
    Compound: AA
    Antiviral activity against Herpes simplex virus type 1 infected in human RD cells assessed as inhibition of virus-induced cytopathic effect
    Antiviral activity against Herpes simplex virus type 1 infected in human RD cells assessed as inhibition of virus-induced cytopathic effect
    [PMID: 22390834]
    SW-620 IC50
    >100 μM
    Compound: L-ASA
    Antiproliferative activity against human SW620 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    Antiproliferative activity against human SW620 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    [PMID: 31586832]
    体外実験

    The anti-cancer effects of L-Ascorbic acid are determined by sodium-dependent vitamin C transporter 2 (SVCT-2), a transporter of L-ascorbic acid. L-Ascorbic acid (0.1 μM-2 mM) exhibits anti-cancer effects according to SVCT-2 expression and L-ascorbic acid uptake. Human colorectal cancer cell lines displays differential responses to L-ascorbic acid, primarily depending on the expression level of SVCT-2[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[4]

    Cell Line: High SVCT-2 expressing cell lines Sw620, Sw480, LoVo, SNU-C4; Low SVCT-2 expressing cell lines HCT15, HCT116, DLD-1, CoLo-205
    Concentration: 0, 0.1 μM, 1 μM, 10 μM, 0.1 mM, 0.5 mM, 1 mM, and 2 mM
    Incubation Time: 24 hours
    Result: Some high SVCT-2 expressing cancer cells demonstrated a dramatic cell-autonomous inhibitory effect of L-ascorbic acid.
    Low SVCT-2 expressing cell lines showed biphasic responses to L-ascorbic acid.

    Western Blot Analysis[4]

    Cell Line: Sw620, Sw480, LoVo, SNU-C4, HCT15, HCT116, DLD-1, CoLo-205 cell lines
    Concentration: 1 mM
    Incubation Time:
    Result: The cell lines showed different levels of SVCT-2 expression in western blot analyses: Sw620, Sw480, and Lovo expressed high levels of SVCT-2 whereas HCT116, HCT15, and DLD-1 expressed low levels.
    体内実験

    L-Ascorbic acid/Tolbutamide produces hypoglycaemic activity in a dose dependant manner in normal (60 mg/kg) and diabetic (40 mg/kg) condition. In the presence of L-ascorbic acid, Tolbuatmide (20 mg/kg) produces early onset of action and maintained for longer period compared to Tolbutamide matching control[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Normal rats:Albino rats of either sex weighing between 125-175 g[5]
    Dosage: Group I received L-ascorbic acid 60 mg/kg, Group II received Tolbutamide 20 mg/kg and Group III was given L-ascorbic acid (60 mg/kg) prior to the administration of tolbutamide 20 mg/kg
    Administration: Administered orally
    Result: L-ascorbic acid at the dose of 60 mg/kg produced 50.91% blood glucose reduction at 0.5 h and 20 mg/kg body weight of Tolbutamide produced 33% at 4 h as peak effects. In the presence of L-ascorbic acid (60 mg/kg), the action of Tolbutamide was early in onset and maintained for 6 h.
    Animal Model: Diabetic rats:Albino rats of either sex weighing between 125 to 175 g were fasted overnight before injection with Alloxan[5]
    Dosage: Group I received L-ascorbic acid 40 mg/kg and Group II received Tolbutamide 20 mg/kg while Group III was given L-ascorbic acid 40 mg/kg prior to Tolbutamide administration (20 mg/kg).
    Administration: Oral administration
    Result: L-ascorbic acid (40 mg/kg alone) produced 42.53% blood glucose reduction at 1.5 h and Tolbutamide 20 mg/kg produced 45.09 at 4 h. Administration of L-ascorbic acid 40 mg/kg body weight prior to Tolbutamide produced antidiabetic activity at 0.5 h and was maintained for 6 h.
    分子量

    176.12

    分子式

    C6H8O6

    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OC([C@@]1([H])[C@H](CO)O)=C(O)C(O1)=O

    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.

    保管条件

    RT, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (RT, protect from light)

    溶剤 & 溶解度
    体外: 

    H2O : ≥ 100 mg/mL (567.79 mM)

    DMSO : 100 mg/mL (567.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.6779 mL 28.3897 mL 56.7795 mL
    5 mM 1.1356 mL 5.6779 mL 11.3559 mL
    10 mM 0.5678 mL 2.8390 mL 5.6779 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

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    一般には略語で表示されます:C1V1 = C2V2

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 5 mg/mL (28.39 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 5 mg/mL (28.39 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (567.79 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.97%

    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 5.6779 mL 28.3897 mL 56.7795 mL 141.9487 mL
    5 mM 1.1356 mL 5.6779 mL 11.3559 mL 28.3897 mL
    10 mM 0.5678 mL 2.8390 mL 5.6779 mL 14.1949 mL
    15 mM 0.3785 mL 1.8926 mL 3.7853 mL 9.4632 mL
    20 mM 0.2839 mL 1.4195 mL 2.8390 mL 7.0974 mL
    25 mM 0.2271 mL 1.1356 mL 2.2712 mL 5.6779 mL
    30 mM 0.1893 mL 0.9463 mL 1.8926 mL 4.7316 mL
    40 mM 0.1419 mL 0.7097 mL 1.4195 mL 3.5487 mL
    50 mM 0.1136 mL 0.5678 mL 1.1356 mL 2.8390 mL
    60 mM 0.0946 mL 0.4732 mL 0.9463 mL 2.3658 mL
    80 mM 0.0710 mL 0.3549 mL 0.7097 mL 1.7744 mL
    100 mM 0.0568 mL 0.2839 mL 0.5678 mL 1.4195 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    一般には略語で表示されます:C1V1 = C2V2

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    製品名:
    L-Ascorbic acid
    製品番号:
    HY-B0166
    数量:
    MCE 日本正規代理店: