Puromycin (Synonyms: CL13900)

Name: Puromycin
Brand: MedChemExpress
SKU: HY-B1743
Rating: 5.0 (387 reviews)

Cat. No.: HY-B1743 Pureza: 99.55%: Ficha de datos
COA

SDS
Instrucciones de manejo
FAQs
Technical Support

Puromycin dihydrochloride is the dihydrochloride salt of puromycin. Puromycin is an aminoglycoside antibiotic that inhibits protein synthesis.

Para uso exclusivo en investigación. No vendemos a pacientes.

No. CAS : 53-79-2

Tamaño	Stock	Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
1 mg	En stock	Estimated Time of Arrival: December 31
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

or Consulta para venta a granel

* Seleccione Cantidad antes de agregar artículos.

This product is a controlled substance and not for sale in your territory.

Revisión del cliente

Based on 387 publication(s) in Google Scholar

Other Forms of Puromycin:

387 Publications Citing Use of MCE Puromycin

Others

: Puromycin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Nov 6;15(1):9578. [Abstract]; Viral titers of supernatants from Huh7.0 FAM134B WT cells, Huh7.0 FAM134B KO cells, Huh7.0 FAM134B KO cells (1 μg/mL, 48 h) stably expressing FAM134BWT or Huh7.0 FAM134B KO cells stably expressing FAM134BR142A, infected with ZIKV at an MOI of 1 for 72 h, quantified by plaque assay.

: Puromycin purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Jun;21(6):1192-1211. [Abstract]; Wild-type or mutated A549 cells subjected to the indicated treatments were stably transfected with a puromycin-inducible GFP-LC3 lentivirus at an MOI of 5. Autophagosomes levels were represented by the presence of GFP-LC3 in cells (A scale bar: 10 μm; B scale bar: 20 μm) (left panel).

: Puromycin purchased from MedChemExpress. Usage Cited in: Mol Cell. 2023 Dec 21;83(24):4494-4508.e6. [Abstract]; Global protein synthesis analyzed by puromycin dihydrochloride (4 d) labeling assay upon METTL14 knockdown in HeLa cells. Silencing of EIF3A serves as a positive control.

: Puromycin purchased from MedChemExpress. Usage Cited in: Mol Cell. 2023 Dec 21;83(24):4494-4508.e6. [Abstract]; Global protein synthesis analyzed by puromycin dihydrochloride (4 d) labeling assay upon IGF2BP1-3 knockdown in HeLa cells.

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas Antibiotic:

Ver todas las isoformas

Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

Ver todos los productos específicos de isoformas Parasite:

Ver todas las isoformas

Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

Actividad biológica
Pureza y Documentación
Referencias
Revisión del cliente

Descripciòn

Puromycin dihydrochloride is the dihydrochloride salt of puromycin. Puromycin is an aminoglycoside antibiotic that inhibits protein synthesis.

IC₅₀ & Target

Aminoglycoside

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.6 3 Compound: Puromycin	Cytotoxicity against human A549 cells Cytotoxicity against human A549 cells	[PMID: 27089214]
A549	IC₅₀	300 1 Compound: 5	Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs	[PMID: 30418763]
A549	IC₅₀	0.6 3 Compound: Puromycin	Cytotoxicity against human A549 cells Cytotoxicity against human A549 cells	[PMID: 27089214]
A549	IC₅₀	300 1 Compound: 5	Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs	[PMID: 30418763]
HEK293	IC₅₀	0.35 1 Compound: Puromycin	Cytotoxicity against HEK293 cells by alamar blue assay Cytotoxicity against HEK293 cells by alamar blue assay	[PMID: 27212070]
HEK293	CC₅₀	0.23 3 Compound: Puromycin	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 48 hrs by resazurin assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 48 hrs by resazurin assay	[PMID: 33479668]
BEAS-2B	IC₅₀	0.74 3 Compound: Puro	Cytotoxicity against human BEAS-2B cells with low TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human BEAS-2B cells with low TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 36855911]
HEK293	IC₅₀	0.35 1 Compound: Puromycin	Cytotoxicity against HEK293 cells by alamar blue assay Cytotoxicity against HEK293 cells by alamar blue assay	[PMID: 27212070]
BEAS-2B	IC₅₀	0.74 3 Compound: Puro	Cytotoxicity against human BEAS-2B cells with low TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human BEAS-2B cells with low TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 36855911]
HEK293	IC₅₀	0.36 3 Compound: Puromycin	Growth inhibition of HEK293 cells after 72 hrs by PrestoBlue staining based fluorescence assay Growth inhibition of HEK293 cells after 72 hrs by PrestoBlue staining based fluorescence assay	[PMID: 28001067]
HCT-8	EC₅₀	6.1 3 Compound: Puromycin	Cytotoxicity against human HCT8 cells Cytotoxicity against human HCT8 cells	[PMID: 29469575]
HEK293	IC₅₀	0.361 3 Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as cell viability after 72 hrs by resazurin-based plate reader analysis Cytotoxicity against HEK293 cells assessed as cell viability after 72 hrs by resazurin-based plate reader analysis	[PMID: 26651537]
HEK293	CC₅₀	0.23 3 Compound: Puromycin	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 48 hrs by resazurin assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 48 hrs by resazurin assay	[PMID: 33479668]
HCT-8	EC₅₀	6.1 3 Compound: Puromycin	Cytotoxicity against human HCT8 cells Cytotoxicity against human HCT8 cells	[PMID: 29469575]
HEK293	IC₅₀	0.42 3 Compound: Puromycin	Cytotoxicity against HEK239 cells after 72 hrs by Alamar blue assay Cytotoxicity against HEK239 cells after 72 hrs by Alamar blue assay	[PMID: 20558060]
HEK293	IC₅₀	0.4 3 Compound: Puromycin	Cytotoxicity against human HEK293 cells after 72 hrs by resazurin dye based fluorescence assay Cytotoxicity against human HEK293 cells after 72 hrs by resazurin dye based fluorescence assay	[PMID: 29969262]
HEK293	IC₅₀	0.36 3 Compound: Puromycin	Cytotoxicity against HEK293 cells Cytotoxicity against HEK293 cells	[PMID: 32067457]
HEK-293T	CC₅₀	0.42 3 Compound: Puromycin	Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 29469575]
HEK293	IC₅₀	0.42 3 Compound: Puromycin	Cytotoxicity against HEK239 cells after 72 hrs by Alamar blue assay Cytotoxicity against HEK239 cells after 72 hrs by Alamar blue assay	[PMID: 20558060]
HEK293	IC₅₀	0.45 3 Compound: Puromycin	Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay	[PMID: 29236492]
HEK-293T	IC₅₀	1.28 3 Compound: Puro	Cytotoxicity against human HEK293T cells with low TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HEK293T cells with low TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 36855911]
HEK293	IC₅₀	0.45 3 Compound: Puromycin	Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay	[PMID: 29236492]
HEK293	CC₅₀	0.23 3 Compound: Puromycin	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 48 hrs by resazurin assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 48 hrs by resazurin assay	[PMID: 33479668]
HEK293	IC₅₀	0.694 3 Compound: Puromycin	Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye-based fluorescence assay Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye-based fluorescence assay	[PMID: 30865443]
HEK293	IC₅₀	0.46 3 Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as inhibition of cell growth after 72 hrs by alamar blue assay Cytotoxicity against HEK293 cells assessed as inhibition of cell growth after 72 hrs by alamar blue assay	[PMID: 29533611]
HEK293	CC₅₀	500.1 1 Compound: Puromycin	Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay	[PMID: 30537832]
HEK293	IC₅₀	0.46 3 Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 72 hrs by Alamar blue assay Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 72 hrs by Alamar blue assay	[PMID: 28774427]
HEK293	IC₅₀	0.46 3 Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 72 hrs by Alamar blue assay Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 72 hrs by Alamar blue assay	[PMID: 28774427]
HEK293	IC₅₀	0.361 3 Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as cell viability after 72 hrs by resazurin-based plate reader analysis Cytotoxicity against HEK293 cells assessed as cell viability after 72 hrs by resazurin-based plate reader analysis	[PMID: 26651537]
HEK293	IC₅₀	0.6645 3 Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as inhibition of cell growth incubated for 72 hrs by alamar blue dye based fluorescence assay Cytotoxicity against HEK293 cells assessed as inhibition of cell growth incubated for 72 hrs by alamar blue dye based fluorescence assay	[PMID: 36799121]
HEK293	IC₅₀	0.694 3 Compound: Puromycin	Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye-based fluorescence assay Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye-based fluorescence assay	[PMID: 30865443]
HEK293	IC₅₀	0.46 3 Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as inhibition of cell growth after 72 hrs by alamar blue assay Cytotoxicity against HEK293 cells assessed as inhibition of cell growth after 72 hrs by alamar blue assay	[PMID: 29533611]
HEK293	IC₅₀	0.81 3 Compound: Puromycin	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay	[PMID: 33105995]
HEK293	IC₅₀	0.6645 3 Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as inhibition of cell growth incubated for 72 hrs by alamar blue dye based fluorescence assay Cytotoxicity against HEK293 cells assessed as inhibition of cell growth incubated for 72 hrs by alamar blue dye based fluorescence assay	[PMID: 36799121]
HEK293	IC₅₀	0.35 1 Compound: Puromycin	Cytotoxicity against HEK293 cells by alamar blue assay Cytotoxicity against HEK293 cells by alamar blue assay	[PMID: 27212070]
HEK293	IC₅₀	350.5 1 Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as growth inhibition after 72 hrs by alamar blue viability assay Cytotoxicity against HEK293 cells assessed as growth inhibition after 72 hrs by alamar blue viability assay	[PMID: 27060763]
HEK293	IC₅₀	0.4 3 Compound: Puromycin	Cytotoxicity against human HEK293 cells after 72 hrs by resazurin dye based fluorescence assay Cytotoxicity against human HEK293 cells after 72 hrs by resazurin dye based fluorescence assay	[PMID: 29969262]
HEK293	IC₅₀	0.49 3 Compound: Puromycin	Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability measured after 66 hrs by Alamar Blue dye based fluorescence analysis Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability measured after 66 hrs by Alamar Blue dye based fluorescence analysis	[PMID: 38416027]
HEK293	IC₅₀	0.36 3 Compound: Puromycin	Growth inhibition of HEK293 cells after 72 hrs by PrestoBlue staining based fluorescence assay Growth inhibition of HEK293 cells after 72 hrs by PrestoBlue staining based fluorescence assay	[PMID: 28001067]
HEK293	CC₅₀	500.1 1 Compound: Puromycin	Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay	[PMID: 30537832]
HEK293	IC₅₀	0.36 3 Compound: Puromycin	Cytotoxicity against HEK293 cells Cytotoxicity against HEK293 cells	[PMID: 32067457]
HEK293	IC₅₀	0.81 3 Compound: Puromycin	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay	[PMID: 33105995]
HEK-293T	CC₅₀	0.42 3 Compound: Puromycin	Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 29469575]
HEK293	IC₅₀	0.361 3 Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as cell viability after 72 hrs by resazurin-based plate reader analysis Cytotoxicity against HEK293 cells assessed as cell viability after 72 hrs by resazurin-based plate reader analysis	[PMID: 26651537]
HepG2	IC₅₀	0.23 3 Compound: puromycin	Growth inhibition of human HepG2 cells after 72 hrs by MTT assay Growth inhibition of human HepG2 cells after 72 hrs by MTT assay	[PMID: 18313307]
HEK293	IC₅₀	0.399 3 Compound: Puromycin	Growth inhibition of HEK293 cells Growth inhibition of HEK293 cells	[PMID: 28400231]
HEK293	IC₅₀	0.399 3 Compound: Puromycin	Growth inhibition of HEK293 cells Growth inhibition of HEK293 cells	[PMID: 28400231]
HEK293	IC₅₀	0.36 3 Compound: Puromycin	Growth inhibition of HEK293 cells after 72 hrs by PrestoBlue staining based fluorescence assay Growth inhibition of HEK293 cells after 72 hrs by PrestoBlue staining based fluorescence assay	[PMID: 28001067]
HepG2	CC₅₀	0.83 3 Compound: Puromycin	Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 29469575]
HEK293	IC₅₀	0.4 3 Compound: Puromycin	Cytotoxicity against human HEK293 cells after 72 hrs by resazurin dye based fluorescence assay Cytotoxicity against human HEK293 cells after 72 hrs by resazurin dye based fluorescence assay	[PMID: 29969262]
HEK-293T	CC₅₀	0.42 3 Compound: Puromycin	Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 29469575]
HepG2	IC₅₀	2.1 x 10^-1 3 Compound: puromycin	Growth inhibition of human HepG2 cells after 72 hrs by SRB assay Growth inhibition of human HepG2 cells after 72 hrs by SRB assay	[PMID: 18512984]
HEK293	IC₅₀	0.42 3 Compound: Puromycin	Cytotoxicity against HEK239 cells after 72 hrs by Alamar blue assay Cytotoxicity against HEK239 cells after 72 hrs by Alamar blue assay	[PMID: 20558060]
HEK-293T	IC₅₀	1.28 3 Compound: Puro	Cytotoxicity against human HEK293T cells with low TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HEK293T cells with low TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 36855911]
HepG2	IC₅₀	83.93 3 Compound: puromycin	Growth inhibition of doxorubicin resistant human HepG2 cells after 72 hrs by MTT assay Growth inhibition of doxorubicin resistant human HepG2 cells after 72 hrs by MTT assay	[PMID: 18313307]
HeLa	IC₅₀	0.9 3 Compound: Puro	Cytotoxicity against human HeLa cells with high TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HeLa cells with high TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 36855911]
HL-60	EC₅₀	0.055 3 Compound: 1	Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay	[PMID: 28803047]
HEK293	IC₅₀	0.45 3 Compound: Puromycin	Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay	[PMID: 29236492]
HEK293	IC₅₀	0.46 3 Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 72 hrs by Alamar blue assay Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 72 hrs by Alamar blue assay	[PMID: 28774427]
Hepatocyte	CC₅₀	1.1 3 Compound: Puromycin	Cytotoxicity against human primary hepatocytes assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay Cytotoxicity against human primary hepatocytes assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 37596939]
J774.A1	IC₅₀	10 3 Compound: Puromycin	Cytotoxicity against mouse J774A1 cells by trypan blue exclusion assay Cytotoxicity against mouse J774A1 cells by trypan blue exclusion assay	[PMID: 22934636]
HEK293	IC₅₀	0.46 3 Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as inhibition of cell growth after 72 hrs by alamar blue assay Cytotoxicity against HEK293 cells assessed as inhibition of cell growth after 72 hrs by alamar blue assay	[PMID: 29533611]
HepG2	CC₅₀	0.83 3 Compound: Puromycin	Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 29469575]
K562	IC₅₀	0.22 3 Compound: puromycin	Growth inhibition of human K562 cells after 72 hrs by SRB assay Growth inhibition of human K562 cells after 72 hrs by SRB assay	[PMID: 18313307]
HEK293	IC₅₀	0.49 3 Compound: Puromycin	Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability measured after 66 hrs by Alamar Blue dye based fluorescence analysis Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability measured after 66 hrs by Alamar Blue dye based fluorescence analysis	[PMID: 38416027]
HepG2	CC₅₀	0.78 3 Compound: Puromycin	Cytotoxicity against human HepG2 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay Cytotoxicity against human HepG2 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 37596939]
K562	IC₅₀	26.83 3 Compound: puromycin	Growth inhibition of doxorubicin-resistant human K562 cells after 72 hrs by SRB assay Growth inhibition of doxorubicin-resistant human K562 cells after 72 hrs by SRB assay	[PMID: 18313307]
HEK293	IC₅₀	0.6645 3 Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as inhibition of cell growth incubated for 72 hrs by alamar blue dye based fluorescence assay Cytotoxicity against HEK293 cells assessed as inhibition of cell growth incubated for 72 hrs by alamar blue dye based fluorescence assay	[PMID: 36799121]
KB 3-1	IC₅₀	0.21 3 Compound: puromycin	Growth inhibition of human KB3-1 cells after 72 hrs by MTT assay Growth inhibition of human KB3-1 cells after 72 hrs by MTT assay	[PMID: 18313307]
HepG2	IC₅₀	1.29 3 Compound: Puro	Cytotoxicity against human HepG2 cells with high TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HepG2 cells with high TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 36855911]
HepG2	IC₅₀	83.93 3 Compound: puromycin	Growth inhibition of doxorubicin resistant human HepG2 cells after 72 hrs by MTT assay Growth inhibition of doxorubicin resistant human HepG2 cells after 72 hrs by MTT assay	[PMID: 18313307]
KB-V1	IC₅₀	79.3 3 Compound: puromycin	Growth inhibition of human KB V1 cells after 72 hrs by MTT assay Growth inhibition of human KB V1 cells after 72 hrs by MTT assay	[PMID: 18313307]
HEK293	IC₅₀	0.694 3 Compound: Puromycin	Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye-based fluorescence assay Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye-based fluorescence assay	[PMID: 30865443]
HEK293	IC₅₀	0.81 3 Compound: Puromycin	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay	[PMID: 33105995]
MDA-MB-231	CC₅₀	0.48 3 Compound: Puromycin	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay	[PMID: 28523103]
HepG2	IC₅₀	0.23 3 Compound: puromycin	Growth inhibition of human HepG2 cells after 72 hrs by MTT assay Growth inhibition of human HepG2 cells after 72 hrs by MTT assay	[PMID: 18313307]
HEK293	IC₅₀	350.5 1 Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as growth inhibition after 72 hrs by alamar blue viability assay Cytotoxicity against HEK293 cells assessed as growth inhibition after 72 hrs by alamar blue viability assay	[PMID: 27060763]
HepG2	IC₅₀	2.1 × 10^-1 3 Compound: puromycin	Growth inhibition of human HepG2 cells after 72 hrs by SRB assay Growth inhibition of human HepG2 cells after 72 hrs by SRB assay	[PMID: 18512984]
MIA PaCa-2	CC₅₀	0.2 3 Compound: Puromycin	Cytotoxicity against human MIAPaCa2 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay Cytotoxicity against human MIAPaCa2 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay	[PMID: 28523103]
HEK293	IC₅₀	837 1 Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as reduction in cell viability measured after 72 hrs by resazurin dye based fluorescence assay Cytotoxicity against HEK293 cells assessed as reduction in cell viability measured after 72 hrs by resazurin dye based fluorescence assay	[PMID: 37610242]
HepG2	IC₅₀	364 3 Compound: puromycin	Resistant ratio, IC50 for multidrug resistant human HepG2 cells to IC50 for human HepG2 cells Resistant ratio, IC50 for multidrug resistant human HepG2 cells to IC50 for human HepG2 cells	[PMID: 18313307]
MOLT-4	EC₅₀	0.17 3 Compound: 1	Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay	[PMID: 28803047]
HL-60	EC₅₀	0.055 3 Compound: 1	Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay	[PMID: 28803047]
PC-3	IC₅₀	300 1 Compound: 5	Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs	[PMID: 30418763]
HL-60	EC₅₀	0.055 3 Compound: 1	Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay	[PMID: 28803047]
SU-DHL10	CC₅₀	0.11 3 Compound: Puromycin	Cytotoxicity against human SU-DHL10 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay Cytotoxicity against human SU-DHL10 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay	[PMID: 28523103]
HL-60	IC₅₀	3.73 3 Compound: Puro	Cytotoxicity against human HL-60 cells with low TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HL-60 cells with low TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 36855911]
HL-60	IC₅₀	3.73 3 Compound: Puro	Cytotoxicity against human HL-60 cells with low TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HL-60 cells with low TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 36855911]
Huh-7	CC₅₀	0.43 3 Compound: Puromycin	Cytotoxicity against human Huh-7 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay Cytotoxicity against human Huh-7 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 37596939]
Vero	CC₅₀	2.8 3 Compound: 1	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell proliferation after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell proliferation after 72 hrs by MTT assay	[PMID: 28803047]
HeLa	IC₅₀	0.9 3 Compound: Puro	Cytotoxicity against human HeLa cells with high TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HeLa cells with high TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 36855911]
Huh-7	CC₅₀	6.63 3 Compound: GNF-Pf-2016	NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7) NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)	[PMID: 18579783]
J774.A1	IC₅₀	10 3 Compound: Puromycin	Cytotoxicity against mouse J774A1 cells by trypan blue exclusion assay Cytotoxicity against mouse J774A1 cells by trypan blue exclusion assay	[PMID: 22934636]
HepG2	CC₅₀	0.78 3 Compound: Puromycin	Cytotoxicity against human HepG2 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay Cytotoxicity against human HepG2 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 37596939]
K562	IC₅₀	26.83 3 Compound: puromycin	Growth inhibition of doxorubicin-resistant human K562 cells after 72 hrs by SRB assay Growth inhibition of doxorubicin-resistant human K562 cells after 72 hrs by SRB assay	[PMID: 18313307]
K562	IC₅₀	0.22 3 Compound: puromycin	Growth inhibition of human K562 cells after 72 hrs by SRB assay Growth inhibition of human K562 cells after 72 hrs by SRB assay	[PMID: 18313307]
HepG2	CC₅₀	0.83 3 Compound: Puromycin	Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 29469575]
HepG2	CC₅₀	398 1 Compound: Puromycin	Cytotoxicity against human HepG2 cells incubated for 72 hrs by Hoechst 33342 staining based assay Cytotoxicity against human HepG2 cells incubated for 72 hrs by Hoechst 33342 staining based assay	[PMID: 38262446]
K562	IC₅₀	122 3 Compound: puromycin	Resistant ratio, IC50 for multidrug resistant human K562 cells to IC50 for human K562 cells Resistant ratio, IC50 for multidrug resistant human K562 cells to IC50 for human K562 cells	[PMID: 18313307]
HepG2	IC₅₀	0.23 3 Compound: puromycin	Growth inhibition of human HepG2 cells after 72 hrs by MTT assay Growth inhibition of human HepG2 cells after 72 hrs by MTT assay	[PMID: 18313307]
KB 3-1	IC₅₀	0.21 3 Compound: puromycin	Growth inhibition of human KB3-1 cells after 72 hrs by MTT assay Growth inhibition of human KB3-1 cells after 72 hrs by MTT assay	[PMID: 18313307]
HepG2	IC₅₀	1.29 3 Compound: Puro	Cytotoxicity against human HepG2 cells with high TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HepG2 cells with high TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 36855911]
KB 3-1	IC₅₀	378 3 Compound: puromycin	Resistant ratio, IC50 for multidrug resistant human KB3-1 cells to IC50 for human KB3-1 cells Resistant ratio, IC50 for multidrug resistant human KB3-1 cells to IC50 for human KB3-1 cells	[PMID: 18313307]
HepG2	IC₅₀	2.1 x 10^-1 3 Compound: puromycin	Growth inhibition of human HepG2 cells after 72 hrs by SRB assay Growth inhibition of human HepG2 cells after 72 hrs by SRB assay	[PMID: 18512984]
KB-V1	IC₅₀	79.3 3 Compound: puromycin	Growth inhibition of human KB V1 cells after 72 hrs by MTT assay Growth inhibition of human KB V1 cells after 72 hrs by MTT assay	[PMID: 18313307]
MCF7	IC₅₀	1.06 3 Compound: Puro	Cytotoxicity against human MCF7 cells with low TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human MCF7 cells with low TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 36855911]
HepG2	IC₅₀	364 3 Compound: puromycin	Resistant ratio, IC50 for multidrug resistant human HepG2 cells to IC50 for human HepG2 cells Resistant ratio, IC50 for multidrug resistant human HepG2 cells to IC50 for human HepG2 cells	[PMID: 18313307]
HepG2	IC₅₀	83.93 3 Compound: puromycin	Growth inhibition of doxorubicin resistant human HepG2 cells after 72 hrs by MTT assay Growth inhibition of doxorubicin resistant human HepG2 cells after 72 hrs by MTT assay	[PMID: 18313307]
MDA-MB-231	CC₅₀	0.48 3 Compound: Puromycin	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay	[PMID: 28523103]
MIA PaCa-2	CC₅₀	0.2 3 Compound: Puromycin	Cytotoxicity against human MIAPaCa2 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay Cytotoxicity against human MIAPaCa2 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay	[PMID: 28523103]
Hepatocyte	CC₅₀	1.1 3 Compound: Puromycin	Cytotoxicity against human primary hepatocytes assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay Cytotoxicity against human primary hepatocytes assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 37596939]
MOLT-4	EC₅₀	0.17 3 Compound: 1	Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay	[PMID: 28803047]
MRC5	CC₅₀	0.32 3 Compound: Puromycin	Cytotoxicity against human MRC5 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay Cytotoxicity against human MRC5 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 37596939]
Hepatocyte	CC₅₀	1740 1 Compound: Puromycin	Cytotoxicity against human primary hepatocytes Cytotoxicity against human primary hepatocytes	[PMID: 38262446]
Huh-7	CC₅₀	0.43 3 Compound: Puromycin	Cytotoxicity against human Huh-7 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay Cytotoxicity against human Huh-7 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 37596939]
MT4	CC₅₀	0.12 3 Compound: Puromycin	Cytotoxicity against human MT4 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay Cytotoxicity against human MT4 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 37596939]
A549	IC₅₀	300 1 Compound: 5	Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs	[PMID: 30418763]
Huh-7	CC₅₀	6.63 3 Compound: GNF-Pf-2016	NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7) NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)	[PMID: 18579783]
J774.A1	IC₅₀	10 3 Compound: Puromycin	Cytotoxicity against mouse J774A1 cells by trypan blue exclusion assay Cytotoxicity against mouse J774A1 cells by trypan blue exclusion assay	[PMID: 22934636]
PC-3	IC₅₀	300 1 Compound: 5	Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs	[PMID: 30418763]
K562	IC₅₀	0.22 3 Compound: puromycin	Growth inhibition of human K562 cells after 72 hrs by SRB assay Growth inhibition of human K562 cells after 72 hrs by SRB assay	[PMID: 18313307]
HEK293	IC₅₀	350.5 1 Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as growth inhibition after 72 hrs by alamar blue viability assay Cytotoxicity against HEK293 cells assessed as growth inhibition after 72 hrs by alamar blue viability assay	[PMID: 27060763]
K562	IC₅₀	122 3 Compound: puromycin	Resistant ratio, IC50 for multidrug resistant human K562 cells to IC50 for human K562 cells Resistant ratio, IC50 for multidrug resistant human K562 cells to IC50 for human K562 cells	[PMID: 18313307]
P388	IC₅₀	0.5 6 Compound: Puromycin	Cytotoxicity against P388 mouse leukemia cells Cytotoxicity against P388 mouse leukemia cells	[PMID: 1433208]
K562	IC₅₀	26.83 3 Compound: puromycin	Growth inhibition of doxorubicin-resistant human K562 cells after 72 hrs by SRB assay Growth inhibition of doxorubicin-resistant human K562 cells after 72 hrs by SRB assay	[PMID: 18313307]
HepG2	CC₅₀	398 1 Compound: Puromycin	Cytotoxicity against human HepG2 cells incubated for 72 hrs by Hoechst 33342 staining based assay Cytotoxicity against human HepG2 cells incubated for 72 hrs by Hoechst 33342 staining based assay	[PMID: 38262446]
KB 3-1	IC₅₀	0.21 3 Compound: puromycin	Growth inhibition of human KB3-1 cells after 72 hrs by MTT assay Growth inhibition of human KB3-1 cells after 72 hrs by MTT assay	[PMID: 18313307]
PBMC	CC₅₀	5.05 3 Compound: Puromycin	Cytotoxicity against quiescent human PBMC cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay Cytotoxicity against quiescent human PBMC cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 37596939]
KB 3-1	IC₅₀	378 3 Compound: puromycin	Resistant ratio, IC50 for multidrug resistant human KB3-1 cells to IC50 for human KB3-1 cells Resistant ratio, IC50 for multidrug resistant human KB3-1 cells to IC50 for human KB3-1 cells	[PMID: 18313307]
PBMC	CC₅₀	1.1 3 Compound: Puromycin	Cytotoxicity against stimulated human PBMC cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay Cytotoxicity against stimulated human PBMC cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 37596939]
PC-3	CC₅₀	0.47 3 Compound: Puromycin	Cytotoxicity against human PC-3 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay Cytotoxicity against human PC-3 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 37596939]
KB-V1	IC₅₀	79.3 3 Compound: puromycin	Growth inhibition of human KB V1 cells after 72 hrs by MTT assay Growth inhibition of human KB V1 cells after 72 hrs by MTT assay	[PMID: 18313307]
HEK293	CC₅₀	500.1 1 Compound: Puromycin	Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay	[PMID: 30537832]
MCF7	IC₅₀	1.06 3 Compound: Puro	Cytotoxicity against human MCF7 cells with low TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human MCF7 cells with low TrxR expression assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 36855911]
PC-3	IC₅₀	0.7 3 Compound: Puromycin	Cytotoxicity against human PC3 cells Cytotoxicity against human PC3 cells	[PMID: 27089214]
HEK293	IC₅₀	837 1 Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as reduction in cell viability measured after 72 hrs by resazurin dye based fluorescence assay Cytotoxicity against HEK293 cells assessed as reduction in cell viability measured after 72 hrs by resazurin dye based fluorescence assay	[PMID: 37610242]
MDA-MB-231	CC₅₀	0.48 3 Compound: Puromycin	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay	[PMID: 28523103]
MIA PaCa-2	CC₅₀	0.2 3 Compound: Puromycin	Cytotoxicity against human MIAPaCa2 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay Cytotoxicity against human MIAPaCa2 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay	[PMID: 28523103]
Hepatocyte	CC₅₀	1740 1 Compound: Puromycin	Cytotoxicity against human primary hepatocytes Cytotoxicity against human primary hepatocytes	[PMID: 38262446]
MOLT-4	EC₅₀	0.17 3 Compound: 1	Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay	[PMID: 28803047]
SU-DHL10	CC₅₀	0.11 3 Compound: Puromycin	Cytotoxicity against human SU-DHL10 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay Cytotoxicity against human SU-DHL10 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay	[PMID: 28523103]
MRC5	CC₅₀	0.32 3 Compound: Puromycin	Cytotoxicity against human MRC5 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay Cytotoxicity against human MRC5 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 37596939]
Vero	CC₅₀	2.8 3 Compound: 1	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell proliferation after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell proliferation after 72 hrs by MTT assay	[PMID: 28803047]
MT4	CC₅₀	0.12 3 Compound: Puromycin	Cytotoxicity against human MT4 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay Cytotoxicity against human MT4 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 37596939]
P388	IC₅₀	0.5 6 Compound: Puromycin	Cytotoxicity against P388 mouse leukemia cells Cytotoxicity against P388 mouse leukemia cells	[PMID: 1433208]
PBMC	CC₅₀	1.1 3 Compound: Puromycin	Cytotoxicity against stimulated human PBMC cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay Cytotoxicity against stimulated human PBMC cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 37596939]
PBMC	CC₅₀	5.05 3 Compound: Puromycin	Cytotoxicity against quiescent human PBMC cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay Cytotoxicity against quiescent human PBMC cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 37596939]
PC-3	CC₅₀	0.47 3 Compound: Puromycin	Cytotoxicity against human PC-3 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay Cytotoxicity against human PC-3 cells assessed as reduction in cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 37596939]
PC-3	IC₅₀	0.7 3 Compound: Puromycin	Cytotoxicity against human PC3 cells Cytotoxicity against human PC3 cells	[PMID: 27089214]
PC-3	IC₅₀	300 1 Compound: 5	Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs	[PMID: 30418763]
SU-DHL10	CC₅₀	0.11 3 Compound: Puromycin	Cytotoxicity against human SU-DHL10 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay Cytotoxicity against human SU-DHL10 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay	[PMID: 28523103]
Vero	CC₅₀	2.76 3 Compound: 3	Cytotoxicity against African green monkey Vero cells by MTT assay Cytotoxicity against African green monkey Vero cells by MTT assay	[PMID: 38917665]
Vero	CC₅₀	2.8 3 Compound: 1	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell proliferation after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell proliferation after 72 hrs by MTT assay	[PMID: 28803047]

In Vitro

Puromycin blocks protein synthesis after aminoacyl-sRNA formation, and at the same time it leads to the accumulation of small peptides. Both of these effects appear to be due to the splitting of ribosome-bound peptidyl-sRNA,4 which results in release of incomplete peptide chains.^[1]. Puromycin, an analog of the 3' end of aminoacyl-tRNA, causes premature termination of translation by being linked non-specifically to growing polypeptide chains. Puromycin has two modes of inhibitory action. The first is by acting as an acceptor substrate which attacks peptidyl-tRNA in the P site to form a nascent peptide. The second is by competing with aminoacyl-tRNA for binding to the A' site^[2]. When used in minimal amounts, puromycin incorporation in neosynthesized proteins reflects directly the rate of mRNA translation in vitro. Puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling. It allows the direct evaluation of translation activity in single cells by immunofluorescence microscopy and in heterogenous populations of cells by fluorescenceactivated cell sorting^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

471.51

Fòrmula

C₂₂H₂₉N₇O₅

No. CAS

53-79-2

Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H]1[C@@H](NC([C@@H](N)CC2=CC=C(OC)C=C2)=O)[C@H]([C@H](N3C=NC4=C3N=CN=C4N(C)C)O1)O

Structure Classification

Initial Source

Microorganisms

Streptomyces alboniger

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (212.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1 mg/mL (2.12 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1208 mL	10.6042 mL	21.2085 mL
5 mM	0.4242 mL	2.1208 mL	4.2417 mL
10 mM	0.2121 mL	1.0604 mL	2.1208 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (10.60 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (10.60 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (10.60 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 99.55%

Select Batch:

Ficha de datos (277 KB) SDS (419 KB)

COA (253 KB) HNMR (501 KB) LCMS (97 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Nathans D, et al. Puromycin inhibition of protein synthesis: incorporation of puromycin intopeptide chains. Proc Natl Acad Sci U S A. 1964 Apr;51:585-92. [Content Brief]

[2]. Miyamoto-Sato E, et al. Specific bonding of puromycin to full-length protein at the C-terminus. Nucleic Acids Res. 2000 Mar 1;28(5):1176-82. [Content Brief]

[3]. Schmidt EK, et al. SUnSET, a nonradioactive method to monitor protein synthesis. Nat Methods. 2009 Apr;6(4):275-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.1208 mL	10.6042 mL	21.2085 mL	53.0211 mL
DMSO	5 mM	0.4242 mL	2.1208 mL	4.2417 mL	10.6042 mL
	10 mM	0.2121 mL	1.0604 mL	2.1208 mL	5.3021 mL
	15 mM	0.1414 mL	0.7069 mL	1.4139 mL	3.5347 mL
	20 mM	0.1060 mL	0.5302 mL	1.0604 mL	2.6511 mL
	25 mM	0.0848 mL	0.4242 mL	0.8483 mL	2.1208 mL
	30 mM	0.0707 mL	0.3535 mL	0.7069 mL	1.7674 mL
	40 mM	0.0530 mL	0.2651 mL	0.5302 mL	1.3255 mL
	50 mM	0.0424 mL	0.2121 mL	0.4242 mL	1.0604 mL
	60 mM	0.0353 mL	0.1767 mL	0.3535 mL	0.8837 mL
	80 mM	0.0265 mL	0.1326 mL	0.2651 mL	0.6628 mL
	100 mM	0.0212 mL	0.1060 mL	0.2121 mL	0.5302 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Puromycin Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Puromycin53-79-2CL13900CL 13900CL-13900BacterialAntibioticParasiteInhibitorinhibitorinhibit

Please select a local distributor ナミキ商事株式会社コスモ・バイオ株式会社重松貿易株式会社
Nombre del producto			Requested Quantity *
Nombre del solicitante *			Saludo
Direcciòn del E-mail *			Número de teléfono *
Department			Nombre de la Organizaciòn *
City			Provincia
Country or Region *
Observaciones

Nombre del producto			Lot #
Nombre del solicitante *			Direcciòn del E-mail *
Número de teléfono			Department *
Nombre de la Organizaciòn *			Country or Region *
Observaciones

Name
E-mail *

	Sorry, but the email address you supplied was invalid.

Puromycin (Synonyms: CL13900)

Revisión del cliente

Other Forms of Puromycin:

Puromycin Related Antibodies

387 Publications Citing Use of MCE Puromycin

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas Antibiotic:

Ver todos los productos específicos de isoformas Parasite:

Actividad biológica

Pureza y Documentación

Referencias

Revisión del cliente

Puromycin Related Antibodies

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

Puromycin Related Classifications

Keywords:

Productos vistos recientemente:

Revisión del cliente

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report