2. Metabolic Enzyme/Protease Cell Cycle/DNA Damage Apoptosis
  3. Endogenous Metabolite Nucleoside Antimetabolite/Analog Caspase Apoptosis
  4. 2'-Deoxyadenosine

2'-デオキシアデノシン  (Synonyms: 2'-Deoxyadenosine)

製品番号: HY-W040329 純度: 99.97%
COA 取扱説明書 Technical Support

2′-Deoxyadenosine is an adenine nucleoside that inhibits glucose-stimulated insulin release. 2′-Deoxyadenosine inhibits glucose-stimulated increases seen in islet cyclic AMP (cAMP) accumulation. 2'-Deoxyadenosine activates caspase-3 and promotes apoptosis. 2'-Deoxyadenosine inhibits the activity of S-adenosyl-L-homocysteine hydrolase (SAHH). 2'-Deoxyadenosine inhibits the growth of various cells. 2'-Deoxyadenosine has an anticancer effect on colon cancer.

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2'-Deoxyadenosine

2'-デオキシアデノシン 構造式

CAS 番号 : 958-09-8

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10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 在庫あり
Solution
10 mM * 1 mL in DMSO USD 55 在庫あり
Solid
100 mg $50 在庫あり
500 mg $70 在庫あり
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Other Forms of 2'-Deoxyadenosine:

2'-デオキシアデノシン 関連抗体

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Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
Human Endogenous Metabolite Microbial Metabolite

Caspase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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製品説明

2′-Deoxyadenosine is an adenine nucleoside that inhibits glucose-stimulated insulin release. 2′-Deoxyadenosine inhibits glucose-stimulated increases seen in islet cyclic AMP (cAMP) accumulation. 2'-Deoxyadenosine activates caspase-3 and promotes apoptosis. 2'-Deoxyadenosine inhibits the activity of S-adenosyl-L-homocysteine hydrolase (SAHH). 2'-Deoxyadenosine inhibits the growth of various cells. 2'-Deoxyadenosine has an anticancer effect on colon cancer[1][2][3].

IC50 & Target

Human Endogenous Metabolite

 

Cellular Effect
Cell Line Type Value Description References
ATH-8 cell line ED50
>100 μM
Compound: 4c
Protection of ATH8 cells against the cytopathic effect of HIV.
Protection of ATH8 cells against the cytopathic effect of HIV.
[PMID: 3497272]
C6 IC50
>250 μM
Compound: 2'-Deoxyadenosine
Cytotoxicity against rat C6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against rat C6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
DU-145 IC50
>250 μM
Compound: 2'-Deoxyadenosine
Cytotoxicity against human DU145 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against human DU145 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
HaCaT IC50
>250 μM
Compound: 2'-Deoxyadenosine
Cytotoxicity against human HaCaT cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against human HaCaT cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
L6 IC50
>250 μM
Compound: 2'-Deoxyadenosine
Cytotoxicity against rat L6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against rat L6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
MCF7 IC50
>250 μM
Compound: 2'-Deoxyadenosine
Cytotoxicity against human MCF7 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
10.1039/C3MD00159H
MT4 ED50
>100 μM
Compound: 11a
Dose required to inhibit cytopathic effect of human immunodeficiency virus replication in MT-4 cells.
Dose required to inhibit cytopathic effect of human immunodeficiency virus replication in MT-4 cells.
[PMID: 3172142]
S49 IC50
180 μM
Compound: dAdo
Compound evaluated for the inhibitory concentration of S49 Mouse Lymphoma T-cells
Compound evaluated for the inhibitory concentration of S49 Mouse Lymphoma T-cells
[PMID: 3874961]
体外実験

2'-Deoxyadenosine (10 mM) does not inhibit insulin biosynthesis or [U-14C]glucose oxidation at concentrations of the nucleosides that gave maximal inhibition of insulin release. 2'-Deoxyadenosine monohydrate produces marked inhibition of the glucose-stimulated increases seen in islet cyclic AMP accumulation[1].
2'-Deoxyadenosine (0.01-0.5 mM; 24-72 h) inhibits the growth of LoVo human colon carcinoma cells in a concentration- and time-dependent manner and promotes apoptosis, as indicated by characteristic apoptotic morphology[2].
2'-Deoxyadenosine inhibits S-adenosyl-L-homocysteine hydrolase activity in crude extracts of L1210 leukemia cells in a time-dependent manner when assays are conducted in the presence of Deoxycoformycin (HY-A0006)[3].
2'-Deoxyadenosine (0.1-2 mM; up to 3 h) inhibits pig keratinocyte outgrowth, [3H] thymidine uptake, and mitosis[4].
2'-Deoxyadenosine (0.1 mM; 4 days) in combination with 1 μM dCF inhibits the growth of LoVo cells in a cell-density-dependent manner[5].
2'-Deoxyadenosine (100 μM; 3 days) in the presence of 3 μM Deoxycoformycin (HY-A0006) is toxic to rat chromaffin cells, causing apoptosis-like cell death, as shown by morphological changes, DNA fragmentation, and increased dATP levels[6].
2'-Deoxyadenosine (0.1 mM) in the presence of 0.01 mM dCF in vitro leads to a significant increase in dATP levels in mouse embryos[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2'-デオキシアデノシン 関連抗体

Apoptosis Analysis[2]

Cell Line: LoVo human colon carcinoma cell line
Concentration: 0.01 mM, 0.02 mM, 0.05 mM, 0.1 mM, 0.2 mM, 0.5 mM
Incubation Time: 24 h, 48 h, 72 h
Result: Increased the percentage of apoptotic cells, reaching a maximum of approximately 25% after 72 h (0.5 mM).
Processed procaspase-3 and released cytochrome c.
分子量

251.25

分子式

C10H13N5O3
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

NC1=NC=NC2=C1N=CN2[C@H]3C[C@H](O)[C@@H](CO)O3
Structure Classification
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (398.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 16.67 mg/mL (66.35 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9801 mL 19.9005 mL 39.8010 mL
5 mM 0.7960 mL 3.9801 mL 7.9602 mL
10 mM 0.3980 mL 1.9900 mL 3.9801 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (9.95 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (9.95 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
純度とドキュメンテーション

純度: 99.97%

COA (254 KB) HNMR (245 KB) RP-HPLC (245 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.9801 mL 19.9005 mL 39.8010 mL 99.5025 mL
5 mM 0.7960 mL 3.9801 mL 7.9602 mL 19.9005 mL
10 mM 0.3980 mL 1.9900 mL 3.9801 mL 9.9502 mL
15 mM 0.2653 mL 1.3267 mL 2.6534 mL 6.6335 mL
20 mM 0.1990 mL 0.9950 mL 1.9900 mL 4.9751 mL
25 mM 0.1592 mL 0.7960 mL 1.5920 mL 3.9801 mL
30 mM 0.1327 mL 0.6633 mL 1.3267 mL 3.3167 mL
40 mM 0.0995 mL 0.4975 mL 0.9950 mL 2.4876 mL
50 mM 0.0796 mL 0.3980 mL 0.7960 mL 1.9900 mL
60 mM 0.0663 mL 0.3317 mL 0.6633 mL 1.6584 mL
DMSO 80 mM 0.0498 mL 0.2488 mL 0.4975 mL 1.2438 mL
100 mM 0.0398 mL 0.1990 mL 0.3980 mL 0.9950 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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2'-Deoxyadenosine Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2'-Deoxyadenosine958-09-8Endogenous MetaboliteNucleoside Antimetabolite/AnalogCaspaseApoptosisLoVoL1210keratinocytecolon carcinomaanticancerInhibitorinhibitorinhibit

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2'-Deoxyadenosine
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