Lacto-N-fucopentaose I
Based on 1 Customer Validation
Lacto-N-fucopentaose I (LNFPI) is a human milk oligosaccharide (HMO), possessing antiviral and antibacterial activity. Lacto-N-fucopentaose I can reduce capsid protein VP1 to block virus adsorption, promote CDK2 and reduce cyclin E to recover cell cycle S phase block. Lacto-N-fucopentaose I inhibits ROS production and apoptosis in virus-infected cells. Lacto-N-fucopentaose I can also regulate intestinal microbiota to affect immune system development.
For research use only. We do not sell to patients.
- Purity : 98.0%
- CAS No.: 7578-25-8
- Formula: C32H55NO25
- Molecular Weight:853.77
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Endogenous Metabolite Isoforms
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Biological Activity
Description
IC50 & Target
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Human Endogenous Metabolite |
CDK2/cyclinE |
In Vitro
Lacto-N-fucopentaose I (25-3200 μg/mL;48 h) exhibits certain cytotoxicity at 3200 μg/mL but no toxic reaction below 1600 μg/mL[1].
Lacto-N-fucopentaose I (25-1600 μg/mL; 14-18 h) can protect EV71-infected RD cells from death[1].
Lacto-N-fucopentaose I (100-400 μg/mL; 16 h) decreases markedly mRNA levels of VP1 and ROS production in EV71-infected RD cells at 400 μg/mL; leads to the recovery of EV71-induced S phase arrest in RD cells[1].
Lacto-N-fucopentaose I (100 and 200 μg/mL; 3 days) inhibits cell apoptosis in Caenorhabditis elegans; significantly decreases the levels of Egl-1, Ced-3 and Ced-4[1].
Lacto-N-fucopentaose I can reduce the abundance of Sphingobacterium, Stenotrophomonas and Achromobacter; can increase the abundance of Micromonospora, Vibrio, Acidibacter, Gaiella, Devosia, Steroidobacter, Variibacter, Dactylosporangium, RB41, Pir4_lineage, Pirellula, Haliangium, Roseiflexus, Pedomicrobium, and Bradyrhizobium.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RD cells
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Concentration:25, 50, 100, 200, 400, 800, 1600 and 3200 μg/mL
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Incubation Time:48 h
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Result:Exhibited certain cytotoxicity at 3200 μg/mL but no toxic reaction below 1600 μg/mL.
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Cell Line:RD cells (infected with EV71)
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Concentration:100, 200 and 400 μg/mL
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Incubation Time:16 h
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Result:Decreased markedly mRNA levels of VP1 only at 400 μg/mL.
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Cell Line:RD cells (infected with EV71)
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Concentration:100, 200 and 400 μg/mL
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Incubation Time:16 h
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Result:Decreased the rate of cells in early apoptosis to 10.9% ± 1.26% at 400 μg/mL, while the untreated EV71 group was 27.7% ± 2.13%.
Significantly inhibited the activity of caspase-3, caspase-8 and caspase-9.
Recovered the decreased mRNA expression of PAPR, NF-κB and Bcl-2 and properly regulated Bad and Fas into their normal levels.
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Cell Line:RD cells (infected with EV71)
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Concentration:100, 200 and 400 μg/mL
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Incubation Time:12 h
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Result:Rescued EV71-induced S phase arrest, which promoted the transition of the G1 phase to the S phase.
Chemical Information
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CAS No. 7578-25-8
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Appearance Solid
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Molecular Weight 853.77
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Formula C32H55NO25
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Color White to off-white
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SMILES
CC(N[C@@H]1[C@H]([C@@H]([C@@H](CO)O[C@H]1O[C@]2([H])[C@H]([C@@H](O[C@H](CO)[C@@H]2O)O[C@@]([C@H](O)CO)([H])[C@H](O)[C@@H](O)C=O)O)O)O[C@@]3([H])[C@@H]([C@H]([C@@H](O)[C@@H](CO)O3)O)O[C@@]4([H])[C@H]([C@@H]([C@H](O)[C@H](C)O4)O)O)=O
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Synonyms
LNFP I
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Structure Classification
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Initial Source
human milk
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
H2O : 125 mg/mL (146.41 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (285 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.1713 mL | 5.8564 mL | 11.7128 mL | 29.2819 mL |
| 5 mM | 0.2343 mL | 1.1713 mL | 2.3426 mL | 5.8564 mL | |
| 10 mM | 0.1171 mL | 0.5856 mL | 1.1713 mL | 2.9282 mL | |
| 15 mM | 0.0781 mL | 0.3904 mL | 0.7809 mL | 1.9521 mL | |
| 20 mM | 0.0586 mL | 0.2928 mL | 0.5856 mL | 1.4641 mL | |
| 25 mM | 0.0469 mL | 0.2343 mL | 0.4685 mL | 1.1713 mL | |
| 30 mM | 0.0390 mL | 0.1952 mL | 0.3904 mL | 0.9761 mL | |
| 40 mM | 0.0293 mL | 0.1464 mL | 0.2928 mL | 0.7320 mL | |
| 50 mM | 0.0234 mL | 0.1171 mL | 0.2343 mL | 0.5856 mL | |
| 60 mM | 0.0195 mL | 0.0976 mL | 0.1952 mL | 0.4880 mL | |
| 80 mM | 0.0146 mL | 0.0732 mL | 0.1464 mL | 0.3660 mL | |
| 100 mM | 0.0117 mL | 0.0586 mL | 0.1171 mL | 0.2928 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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