2. Apoptosis Stem Cell/Wnt MAPK/ERK Pathway NF-κB Metabolic Enzyme/Protease
  3. Caspase ERK Keap1-Nrf2 Heme Oxygenase (HO) Apoptosis
  4. Senkyunolide I

Senkyunolide I is an orally active, blood-brain barrier-permeable metabolite of Z-ligustilide (HY-N0401A). Senkyunolide I is isolated from Ligusticum chuanxiong. Senkyunolide I upregulates p-Erk1/2 and Nrf2/HO-1, and inhibits Caspase 3. Senkyunolide I alleviates Apoptosis. Senkyunolide I increases the pain threshold in mice and reduces acetic acid-induced writhing responses in mice. Senkyunolide I improves neurological deficits, reduces infarct volume and alleviates cerebral edema in rats with focal cerebral ischemia-reperfusion injury. Senkyunolide I protects renal function and structural integrity in a mouse model of renal ischemia-reperfusion injury. Senkyunolide I is applicable to research related to focal cerebral ischemia-reperfusion injury, migraine, and renal ischemia-reperfusion injury.

For research use only. We do not sell to patients.

Senkyunolide I

Senkyunolide I Chemical Structure

CAS No. : 94596-28-8

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Senkyunolide I Related Antibodies

Top Publications Citing Use of Products

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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ERK ERK1 ERK2 ERK5 ERK8

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HO-1 HO-2

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Senkyunolide I is an orally active, blood-brain barrier-permeable metabolite of Z-ligustilide (HY-N0401A). Senkyunolide I is isolated from Ligusticum chuanxiong. Senkyunolide I upregulates p-Erk1/2 and Nrf2/HO-1, and inhibits Caspase 3. Senkyunolide I alleviates Apoptosis. Senkyunolide I increases the pain threshold in mice and reduces acetic acid-induced writhing responses in mice. Senkyunolide I improves neurological deficits, reduces infarct volume and alleviates cerebral edema in rats with focal cerebral ischemia-reperfusion injury. Senkyunolide I protects renal function and structural integrity in a mouse model of renal ischemia-reperfusion injury. Senkyunolide I is applicable to research related to focal cerebral ischemia-reperfusion injury, migraine, and renal ischemia-reperfusion injury[1][2][3].

IC50 & Target[1]

ERK1

 

ERK2

 

Caspase 3

 

HO-1

 

In Vitro

Senkyunolide I (25-100 μM) activates the Nrf2/ARE pathway in Hek293T/pGF1-ARE cells[1].
Senkyunolide I (100-300 μg/mL; prior to H2O2 treatment) protects the renal tubular epithelial cell line HK2 against H2O2-induced loss of viability[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Senkyunolide I Related Antibodies
In Vivo

Senkyunolide I (36-72 mg/kg; i.v.; single dose at 15 minutes post-occlusion) exerts dose-dependent neuroprotective effects against focal cerebral ischemia-reperfusion injury in rats, with the 72 mg/kg dose significantly reducing neurological deficit scores to below vehicle levels, lowering infarct volume to 27.31% ± 2.40%, and enhancing anti-oxidant and anti-apoptotic pathways[1].
Senkyunolide I (8-32 mg/kg; p.o.; single dose) dose-dependently increases thermal pain thresholds in mice, with the 32 mg/kg dose producing significant elevations as early as 20 min post-administration and the 16 mg/kg dose showing significant effects at 360 min post-administration[2].
Senkyunolide I (32 mg/kg; p.o.; single dose) significantly reduces acetic acid-induced visceral pain in mice, while lower doses show no significant effect[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Sprague-Dawley (male, 250-300 g, transient middle cerebral artery occlusion for 2 hours followed by 24 hours of reperfusion)[1]
Dosage: 36 mg/kg; 72 mg/kg
Administration: i.v.; single dose at 15 minutes post-occlusion
Result: Significantly reduced neurological deficit scores at 72 mg/kg (P < 0.05 vs.
vehicle); showed non-significant alleviation of motor function at 36 mg/kg.
Significantly reduced ipsilateral brain water content to 82.30% ± 0.91% at 72 mg/kg (P < 0.05 vs.
vehicle); resulted in non-significant reduction to 85.35% ± 1.29% at 36 mg/kg.
Reduced infarct volume to 35.70% ± 5.35% at 36 mg/kg (P < 0.05 vs.
vehicle); reduced infarct volume to 27.31% ± 2.40% at 72 mg/kg (P < 0.05 vs.
vehicle).
Reversed cerebral morphologic damage in cortices and hippocampus at both 36 mg/kg and 72 mg/kg, with more dramatic reduction of histopathological alterations at 72 mg/kg dose.
Significantly decreased cortical MDA levels (P < 0.05 vs.
vehicle) and significantly increased cortical SOD activities (P < 0.05 vs.
vehicle) at both 36 mg/kg and 72 mg/kg, with no significant differences between the two doses.
Significantly increased the p-Erk1/2/t-Erk1/2 ratio, and the protein levels of c-Nrf2, n-Nrf2, HO-1, and NQO1 (P < 0.05 vs.
vehicle) at both 36 mg/kg and 72 mg/kg; resulted in higher levels of these proteins at 72 mg/kg compared to 36 mg/kg (P < 0.05).
Significantly reversed the down-regulation of Bcl-2 and up-regulation of Bax induced by I/R injury (P < 0.05 vs.
vehicle), elevated the Bcl-2/Bax ratio (P < 0.05 vs.
vehicle), and significantly down-regulated cleaved caspase 3 and caspase 9 expression (P < 0.05 vs.
vehicle) at both 36 mg/kg and 72 mg/kg; more effectively reduced Bax protein levels at 72 mg/kg compared to 36 mg/kg (P < 0.05), with no significant differences between doses for caspase inhibition.
Animal Model: Kunming (female, 18-22 g)[2]
Dosage: 8 mg/kg; 16 mg/kg; 32 mg/kg
Administration: p.o.; single dose
Result: Elevated pain thresholds to 28.0 ± 7.0 s, 32.0 ± 7.3 s, and 35.5 ± 7.3 s at 20, 180, and 240 min post-administration for 32 mg/kg dose (P < 0.05 vs control).
Elevated pain threshold to 28.5 ± 6.9 s at 360 min post-administration for 16 mg/kg dose (P < 0.05 vs control).
Did not produce significant pain threshold elevations at any time point for 8 mg/kg dose.\nReduced the number of abdominal writhing responses to 18.8 ± 4.4 for 32 mg/kg dose (P < 0.05 vs control).
Did not produce significant reductions in writhing responses for 8 mg/kg and 16 mg/kg doses.
Molecular Weight

224.26

Formula

C12H16O4
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C1O/C(C2=C1[C@H](O)[C@@H](O)CC2)=C\CCC
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (445.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4591 mL 22.2956 mL 44.5911 mL
5 mM 0.8918 mL 4.4591 mL 8.9182 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (11.15 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (11.15 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
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%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.37%

SDS (394 KB)

COA (251 KB) HNMR (245 KB) LCMS (89 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.4591 mL 22.2956 mL 44.5911 mL 111.4778 mL
5 mM 0.8918 mL 4.4591 mL 8.9182 mL 22.2956 mL
10 mM 0.4459 mL 2.2296 mL 4.4591 mL 11.1478 mL
15 mM 0.2973 mL 1.4864 mL 2.9727 mL 7.4319 mL
20 mM 0.2230 mL 1.1148 mL 2.2296 mL 5.5739 mL
25 mM 0.1784 mL 0.8918 mL 1.7836 mL 4.4591 mL
30 mM 0.1486 mL 0.7432 mL 1.4864 mL 3.7159 mL
40 mM 0.1115 mL 0.5574 mL 1.1148 mL 2.7869 mL
50 mM 0.0892 mL 0.4459 mL 0.8918 mL 2.2296 mL
60 mM 0.0743 mL 0.3716 mL 0.7432 mL 1.8580 mL
80 mM 0.0557 mL 0.2787 mL 0.5574 mL 1.3935 mL
100 mM 0.0446 mL 0.2230 mL 0.4459 mL 1.1148 mL
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Senkyunolide I Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Senkyunolide I94596-28-8CaspaseERKKeap1-Nrf2Heme Oxygenase (HO)ApoptosisExtracellular signal regulated kinasesHeme Oxygenasecleaved caspase 3Nrf2/HO-1 pathwayBaxSprague-Dawley ratsnitroglycerin-induced migraine model ratscleaved caspase 9Erk1/2Bcl-2HK2 human renal tubular epithelial cellsC57BL/6J miceInhibitorinhibitorinhibit

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