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  4. Folic acid

Folic acid  (Synonyms: 葉酸; Vitamin B9; Vitamin M)

製品番号: HY-16637 純度: 99.02%
COA 取扱説明書 Technical Support

Folic acid (Vitamin B9) is a orally active essential nutrient from the B complex group of vitamins. Folic acid shows antidepressant-like effect. Folic acid sodium reduces the risk of neonatal neural tube defects. Folic acid can be used to the research of megaloblastic and macrocytic anemias due to folic deficiency.

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CAS 番号 : 59-30-3

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 27 在庫あり
Solution
10 mM * 1 mL in DMSO USD 27 在庫あり
Solid
100 mg $25 在庫あり
500 mg $40 在庫あり
1 g $48 在庫あり
5 g $72 在庫あり
10 g $88 在庫あり
25 g $120 在庫あり
50 g $144 在庫あり
100 g $175 在庫あり
> 100 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 25 publication(s) in Google Scholar

Other Forms of Folic acid:

Folic acid 関連抗体

Top Publications Citing Use of Products

    Folic acid purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Dec 8:S2090-1232(25)00996-8.  [Abstract]

    Epithelial junctional proteins were upregulated by Folic acid (30 mg/kg) treated.

    Folic acid purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Dec 8:S2090-1232(25)00996-8.  [Abstract]

    Decreased expression of mesenchymal markers, MMPs, and EMT regulators after Folic acid (30 mg/kg) administration.

    Folic acid purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Dec 8:S2090-1232(25)00996-8.  [Abstract]

    Folic acid (30 mg/kg) restored epithelial architecture, as evidenced by the increase in crypt integrity, maintenance of goblet cells.

    Folic acid purchased from MedChemExpress. Usage Cited in: JCI Insight. 2022 Apr 8;7(7):e152330.  [Abstract]

    mRNA levels of TGF-β1, α-SMA, and fibronectin in the kidney of CTL, Folic acid (FAN) (250 mg/kg, IP, 7 days), and FAN+GsMTx4 mice.

    Folic acid purchased from MedChemExpress. Usage Cited in: JCI Insight. 2022 Apr 8;7(7):e152330.  [Abstract]

    Photomicrographs of Masson’s staining in the kidney of CTL, Folic acid (FAN) (250 mg/kg, IP, 7 days), and FAN+GsMTx4 mice.

    DNA/RNA Synthesis アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Folic acid (Vitamin B9) is a orally active essential nutrient from the B complex group of vitamins. Folic acid shows antidepressant-like effect. Folic acid sodium reduces the risk of neonatal neural tube defects. Folic acid can be used to the research of megaloblastic and macrocytic anemias due to folic deficiency[1][2][3][4].

    IC50 & Target

    Human Endogenous Metabolite

     

    Microbial Metabolite

     

    Cellular Effect
    Cell Line Type Value Description References
    K562 IC50
    290 μM
    Compound: folate
    Ability to inhibit thymidylate synthase derived from human leukemia K562 cells
    Ability to inhibit thymidylate synthase derived from human leukemia K562 cells
    		[PMID: 3470522]
    KB IC50
    0.5 μM
    Compound: folic acid
    Photocytotoxicity against human KB cells overexpressing folate receptor assessed as cell viability incubated for 24 hrs followed by halogen light illumination measured after 20 mins by MTT assay
    Photocytotoxicity against human KB cells overexpressing folate receptor assessed as cell viability incubated for 24 hrs followed by halogen light illumination measured after 20 mins by MTT assay
    		[PMID: 24088087]
    L1210 IC50
    >0.001 M
    Compound: table II
    Inhibitory activity against thymidylate synthase in the intact L1210 cells
    Inhibitory activity against thymidylate synthase in the intact L1210 cells
    		[PMID: 3100797]
    L1210 IC50
    >10 mM
    Compound: Folic acid
    Inhibitory activity against growth of L1210 leukemia cells in culture
    Inhibitory activity against growth of L1210 leukemia cells in culture
    		[PMID: 1560436]
    L1210 IC50
    0.00018 M
    Compound: table II
    Inhibitory activity against the thymidylate synthase in the Permeabilized L1210 cells
    Inhibitory activity against the thymidylate synthase in the Permeabilized L1210 cells
    		[PMID: 3100797]
    L1210 IC50
    0.18 mM
    Compound: table II
    Inhibitory activity against the thymidylate synthase in the Permeabilized L1210 cells
    Inhibitory activity against the thymidylate synthase in the Permeabilized L1210 cells
    		[PMID: 3100797]
    MOLT-4 IC50
    >100 μM
    Compound: Folic acid
    Compound was evaluated for inhibition of MTX influx into MOLT-4 cells
    Compound was evaluated for inhibition of MTX influx into MOLT-4 cells
    		[PMID: 1895294]
    体外実験

    Folic acid plays a critical role in the prevention of chromosome breakage and hypomethylation of DNA[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Folic acid 関連抗体
    体内実験

    Note:
    Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

    Folic acid can be used to induce acute kidney injury models. In rats, oral administration results in an AUC of 1.4 μg h/mL, with an oral bioavailability of 35%[5].

    Induction of Acute Kidney Injury (AKI)[6]
    Background
    Folic acid metabolism requires higher levels of NADPH to reduce folate to THF decreasing the antioxidant defense. The redox imbalance generated by folic acid metabolism is one of the main mechanisms involved in renal damage.
    Specific Modeling Methods
    Rat: Wistar • male
    Administration: 300mg/ml • i.p. • single dose
    Note
    (1) Intraperitoneal injection 300 mg/kg folic acid (dissolved in 300 mM NaHCO ) in male Wistar rats with an initial body weight between 230 to 250 g.
    (2) Plasma was collected and analyzed at days 2, 4, 7, 14 and 28 after folic acid administration.
    Modeling Indicators
    Metabolic changes : Assessment of renal injury by blood urea nitrogen (BUN) and plasma creatinine.
    Individual phenotypic change: The ratio of kidney weight to total rat weight was detected.
    Correlated Product(s): Acetylcysteine (HY-B0215)

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 30-40 g swiss mice[2]
    Dosage: 10, 50, 100 mg/kg
    Administration: Oral administration
    Result: Decreased the immobility time in the forced swimming test (FST) (F324=11.21) and produced a significant effect in the immobility time in the tail suspension test (TST) (F3,20=5.71).
    Animal Model: 30-40 g swiss mice[2]
    Dosage: 1-10 nmol/site
    Administration: Intracerebroventrical injection
    Result: Decreased the immobility time of mice in the FST (F3,22=12.31) and TST (F3,22=5.50).
    Animal Model: Virgin female Wistar rats[3]
    Dosage: 1, 5 mg/kg (180 g/kg protein with 1 mg/kg folic acid or 90 g/kg casein with 1, 5 mg/kg folic acid)
    Administration: Oral administration
    Result: Prevented epigenetic modification of hepatic gene expression in the offspring.
    臨床実験
    分子量

    441.40

    分子式

    C19H19N7O6
    CAS 番号
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(O)CC[C@@H](C(O)=O)NC(C1=CC=C(NCC2=CN=C3N=C(N)NC(C3=N2)=O)C=C1)=O
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    RT, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (RT, protect from light)
    溶剤 & 溶解度
    体外: 

    1M NaOH : 100 mg/mL (226.55 mM; Need ultrasonic)

    DMSO : 33.33 mg/mL (75.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2655 mL 11.3276 mL 22.6552 mL
    5 mM 0.4531 mL 2.2655 mL 4.5310 mL
    10 mM 0.2266 mL 1.1328 mL 2.2655 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  300 mM Sodium bicarbonate in Water

      Solubility: 50 mg/mL (113.28 mM); Clear solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (4.71 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.40%

    COA (245 KB) HNMR (132 KB) RP-HPLC (180 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / 1M NaOH 1 mM 2.2655 mL 11.3276 mL 22.6552 mL 56.6380 mL
    5 mM 0.4531 mL 2.2655 mL 4.5310 mL 11.3276 mL
    10 mM 0.2266 mL 1.1328 mL 2.2655 mL 5.6638 mL
    15 mM 0.1510 mL 0.7552 mL 1.5103 mL 3.7759 mL
    20 mM 0.1133 mL 0.5664 mL 1.1328 mL 2.8319 mL
    25 mM 0.0906 mL 0.4531 mL 0.9062 mL 2.2655 mL
    30 mM 0.0755 mL 0.3776 mL 0.7552 mL 1.8879 mL
    40 mM 0.0566 mL 0.2832 mL 0.5664 mL 1.4159 mL
    50 mM 0.0453 mL 0.2266 mL 0.4531 mL 1.1328 mL
    60 mM 0.0378 mL 0.1888 mL 0.3776 mL 0.9440 mL
    1M NaOH 80 mM 0.0283 mL 0.1416 mL 0.2832 mL 0.7080 mL
    100 mM 0.0227 mL 0.1133 mL 0.2266 mL 0.5664 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Folic acid Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Folic acid59-30-3Vitamin B9 Vitamin MVitamin B 9Vitamin B-9DNA/RNA SynthesisEnvironmental PollutantsEndogenous MetaboliteEnvironmental Contaminantsnutrientntidepressant-likeneural tube defectsfolic deficiencyInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
    HY-16637
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