Epirubicin hydrochloride (Synonyms: 4'-Epidoxorubicin hydrochloride)

Name: Epirubicin hydrochloride
Brand: MedChemExpress
SKU: HY-13624A
Rating: 5.0 (36 reviews)

Cat. No.: HY-13624A Purity: 99.62%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Epirubicin hydrochloride (4'-Epidoxorubicin hydrochloride), a semisynthetic L-arabino derivative of doxorubicin, has an antineoplastic agent by inhibiting Topoisomerase. Epirubicin hydrochloride inhibits DNA and RNA synthesis. Epirubicin hydrochloride is a Forkhead box protein p3 (Foxp3) inhibitor and inhibits regulatory T cell activity.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

CAS No. : 56390-09-1

사이즈	재고	수량

무료 샘플 (0.1 - 0.2 mg)	지금 신청하기
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 36 publication(s) in Google Scholar

Other Forms of Epirubicin hydrochloride:

Epirubicin In-stock
Epirubicin hydrochloride (Standard) In-stock

36 Publications Citing Use of MCE Epirubicin hydrochloride

Cell Proliferation/Viability Assay

In Vivo Efficacy Study

: Epirubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Jan;20(1):51-64. [Abstract]; Comparison of drug (Epirubicin hydrochloride, EPI; Daunorubicin hydrochloride, DNR; Vinorelbine ditartrate, VNR; Oxaliplatin, OXA; Vincristine, VCR; Artemisinin, ART; Colchicine, COL) cytotoxicity to TC1 cells at the indicated doses and time points measured with CCK-8 assays. R.U. (Relative unit) was calculated from the average O.D. values in each condition as the indicator of cell viability (n = 3).

: Epirubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2022 Apr 7;82(7):1297-1312.e8. [Abstract]; CCK8 assay to detect the survival of MCF7 cells transfected with indicated siRNAs and treated with 1 μM EPI (Epirubicin hydrochloride; for 24 h).

: Epirubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2022 Apr 7;82(7):1297-1312.e8. [Abstract]; CCK8 assay to detect the survival of MCF7 cells transfected with indicated siRNAs and treated with 1 μM EPI (Epirubicin hydrochloride; left) for 24 h or 0.5 μM EPI for 0-60 h (right).

: Epirubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2022 Apr 7;82(7):1297-1312.e8. [Abstract]; Indicated MCF7 cells were treated with 1 μM EPI (Epirubicin hydrochloride) for 24 h. The levels of γH2AX were analyzed by immunoblotting (IB). Numbers represent its relative intensities measured by ImageJ.

: Epirubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2022 Apr 7;82(7):1297-1312.e8. [Abstract]; Xenograft assay of lnc15.2-depleted and FL/FL∗-supplemented MDA-MB-231 cells (n = 5 per group). Tumor growth are shown. The arrow indicates each EPI (Epirubicin hydrochloride; 5 mg/Kg; i.p. on days 0, 4, 8 or 9) treatment.

: Epirubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Asia Pac J Clin Oncol. 2023 Jun;19(3):355-364. [Abstract]; The Epirubicin (EPI) + iodine-125 (¹²⁵I) group is significantly decreases the proliferation ability of HepG2 and SMMC7721 cells (cells are cultured for 2-3 weeks).

: Epirubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Asia Pac J Clin Oncol. 2023 Jun;19(3):355-364. [Abstract]; Epirubicin (EPI) promotes iodine-125 (¹²⁵I)-induced downregulation of the WNT pathway and enhances the radiosensitivity of HepG2 and SMMC7721 cells.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All DNA/RNA Synthesis Isoform Specific Products:

View All Isoforms

RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

View All Topoisomerase Isoform Specific Products:

View All Isoforms

Topoisomerase Topo I Topo II DNA gyrase

View All Antibiotic Isoform Specific Products:

View All Isoforms

Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

Biological Activity
Protocol
순도&문서
References
고객리뷰

제품 설명

Epirubicin hydrochloride (4'-Epidoxorubicin hydrochloride), a semisynthetic L-arabino derivative of doxorubicin, has an antineoplastic agent by inhibiting Topoisomerase^[1]. Epirubicin hydrochloride inhibits DNA and RNA synthesis. Epirubicin hydrochloride is a Forkhead box protein p3 (Foxp3) inhibitor and inhibits regulatory T cell activity^[2].

IC₅₀ & Target^[1]

Topoisomerase

In Vitro

Epirubicin hydrochloride (4'-Epidoxorubicin hydrochloride), like doxorubicin, exerts its antitumor effects by complex with DNA, resulting in damage to DNA and interference with the synthesis of DNA, RNA, and proteins. Epirubicin hydrochloride may also affect the integrity and activity of cellular membranes. Maximal cell kill caused by Epirubicin hydrochloride occurs during the S phase of the cell cycle. With higher concentrations effects are also seen in early G2 as well as G1 and M phases^[1].
Epirubicin hydrochloride display antineoplastic activity against most cancer cells. Epirubicin hydrochloride is cytotoxic to Hepatoma G2 cells with IC₅₀ of 1.6 μg/mL at 24 hr. 1.6 μg/mL Epirubicin hydrochloride induces apoptosis of Hep G2 cells, and higher activity of catalase by 50%, Se-dependent glutathione peroxidase by 110%, Cu, Zn-superoxide dismutase by 172% and Mn-superoxide dismutase by 135%. Epirubicin hydrochloride increases the cellular expression of NADPH-CYP 450 reductase, and reduces GST-π expression^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Epirubicin hydrochloride (4'-Epidoxorubicin hydrochloride) are clinically active against a broad range of tumor types, including breast cancer, malignant lymphomas, soft tissue sarcomas, lung cancer, pleural mesothelioma, gastrointestinal cancer, head and neck cancer, ovarian cancer, prostatic carcinoma, transitional bladder carcinoma and so on^[4].
Epirubicin hydrochloride at a dose of 3.5 mg/kg suppresses tumor mass of human breast tumor xenograft R-27 by 74.4 %^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

579.98

화학식

C₂₇H₃₀ClNO₁₁

CAS No.

56390-09-1

Appearance

Solid

Color

Orange to red

SMILES

O=C(C1=C2C(O)=C3[C@@H](O[C@@]4([H])C[C@H](N)[C@@H](O)[C@H](C)O4)C[C@@](C(CO)=O)(O)CC3=C1O)C5=CC=CC(OC)=C5C2=O.Cl

선적

Room temperature in continental US; may vary elsewhere.

보관

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)

용액&용해도

In Vitro:

H₂O : 50 mg/mL (86.21 mM; Need ultrasonic)

DMSO : 25 mg/mL (43.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7242 mL	8.6210 mL	17.2420 mL
5 mM	0.3448 mL	1.7242 mL	3.4484 mL
10 mM	0.1724 mL	0.8621 mL	1.7242 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 5% DMSO 40% PEG300 5% Tween-80 50% Saline
Solubility: ≥ 2.5 mg/mL (4.31 mM); Clear solution
Protocol 2

Add each solvent one by one: 5% DMSO 95% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.31 mM); Clear solution
Protocol 3

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 4

Add each solvent one by one: 1% DMSO 99% Saline
Solubility: ≥ 0.5 mg/mL (0.86 mM); Clear solution

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 1.1 mg/mL (1.90 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

순도&문서

Purity: 99.62%

Select Batch:

Data Sheet (285 KB) SDS (557 KB)

COA (261 KB) HNMR (305 KB) RP-HPLC (246 KB) MS (71 KB)

Handling Instructions (2659 KB)

References

[1]. Cersosimo RJ, et al. Epirubicin: a review of the pharmacology, clinical activity, and adverse effects of an adriamycin analogue. J Clin Oncol. 1986 Mar;4(3):425-39. [Content Brief]

[2]. Kashima H, et al. Epirubicin, Identified Using a Novel Luciferase Reporter Assay for Foxp3 Inhibitors, Inhibits Regulatory T Cell Activity. PLoS One. 2016 Jun 10;11(6):e0156643. [Content Brief]

[3]. Ozkan, A., et al. Epirubicin HCl toxicity in human-liver derived hepatoma G2 cells. Pol J Pharmacol, 2004. 56(4): p. 435-44. [Content Brief]

[4]. Bonadonna, G., et al. Drugs ten years later: epirubicin. Ann Oncol, 1993. 4(5): p. 359-69. [Content Brief]

[5]. Asanuma, F., et al. Antitumor activity of paclitaxel and epirubicin in human breast carcinoma, R-27. Folia Microbiol (Praha), 1998. 43(5): p. 473-4. [Content Brief]

Cell Assay
^[3]

Hep G2 cells (500 cells/well, monolayer) are plated in a 96-well plate. The next day the cells are treated with Epirubicin in the medium. At the end of the incubation periods, 15% volume of MTT dye solution is added. After 1 hr of incubation at 37°C, an equal volume of solubilization/stop solution (dimethylsul-foxide) is added to each well for an additional 1 hr incubation. The absorbance of the reaction solution at 570 nm is recorded.

MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.

References

[1]. Cersosimo RJ, et al. Epirubicin: a review of the pharmacology, clinical activity, and adverse effects of an adriamycin analogue. J Clin Oncol. 1986 Mar;4(3):425-39. [Content Brief]

[2]. Kashima H, et al. Epirubicin, Identified Using a Novel Luciferase Reporter Assay for Foxp3 Inhibitors, Inhibits Regulatory T Cell Activity. PLoS One. 2016 Jun 10;11(6):e0156643. [Content Brief]

[3]. Ozkan, A., et al. Epirubicin HCl toxicity in human-liver derived hepatoma G2 cells. Pol J Pharmacol, 2004. 56(4): p. 435-44. [Content Brief]

[4]. Bonadonna, G., et al. Drugs ten years later: epirubicin. Ann Oncol, 1993. 4(5): p. 359-69. [Content Brief]

[5]. Asanuma, F., et al. Antitumor activity of paclitaxel and epirubicin in human breast carcinoma, R-27. Folia Microbiol (Praha), 1998. 43(5): p. 473-4. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	1.7242 mL	8.6210 mL	17.2420 mL	43.1049 mL
	5 mM	0.3448 mL	1.7242 mL	3.4484 mL	8.6210 mL
	10 mM	0.1724 mL	0.8621 mL	1.7242 mL	4.3105 mL
	15 mM	0.1149 mL	0.5747 mL	1.1495 mL	2.8737 mL
	20 mM	0.0862 mL	0.4310 mL	0.8621 mL	2.1552 mL
	25 mM	0.0690 mL	0.3448 mL	0.6897 mL	1.7242 mL
	30 mM	0.0575 mL	0.2874 mL	0.5747 mL	1.4368 mL
	40 mM	0.0431 mL	0.2155 mL	0.4310 mL	1.0776 mL
H₂O	50 mM	0.0345 mL	0.1724 mL	0.3448 mL	0.8621 mL
	60 mM	0.0287 mL	0.1437 mL	0.2874 mL	0.7184 mL
	80 mM	0.0216 mL	0.1078 mL	0.2155 mL	0.5388 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Epirubicin hydrochloride Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Epirubicin56390-09-14'-EpidoxorubicinDNA/RNA SynthesisTopoisomeraseApoptosisAntibioticL-arabinoderivativeFoxp3TcellInhibitorinhibitorinhibit

최근 본 상품:

상품명

Requested Quantity *

고객명 *

호칭

메일주소 *

전화번호 *

Department

회사명 *

City

도

Country or Region *

비고

상품명

Lot #

고객명 *

메일주소 *

전화번호

Department *

회사명 *

Country or Region *

비고

Name
이메일 *

	Sorry, but the email address you supplied was invalid.

Epirubicin hydrochloride (Synonyms: 4'-Epidoxorubicin hydrochloride)

고객리뷰

Other Forms of Epirubicin hydrochloride:

Epirubicin hydrochloride Related Antibodies

36 Publications Citing Use of MCE Epirubicin hydrochloride

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All DNA/RNA Synthesis Isoform Specific Products:

View All Topoisomerase Isoform Specific Products:

View All Antibiotic Isoform Specific Products:

Biological Activity

Protocol

순도&문서

References

고객리뷰

Epirubicin hydrochloride Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

Epirubicin hydrochloride Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report