2. Cell Cycle/DNA Damage Apoptosis Anti-infection
  3. DNA/RNA Synthesis Topoisomerase Apoptosis Antibiotic
  4. Epirubicin hydrochloride

エピルビシン 塩酸塩  (Synonyms: Epirubicin (hydrochloride),4'-Epidoxorubicin (hydrochloride))

製品番号: HY-13624A 純度: 99.62%
COA 取扱説明書 Technical Support

Epirubicin hydrochloride (4'-Epidoxorubicin hydrochloride), a semisynthetic L-arabino derivative of doxorubicin, has an antineoplastic agent by inhibiting Topoisomerase. Epirubicin hydrochloride inhibits DNA and RNA synthesis. Epirubicin hydrochloride is a Forkhead box protein p3 (Foxp3) inhibitor and inhibits regulatory T cell activity.

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CAS 番号 : 56390-09-1

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 84 在庫あり
Solution
10 mM * 1 mL in DMSO USD 84 在庫あり
Solid
5 mg $66 在庫あり
10 mg $106 在庫あり
25 mg $212 在庫あり
50 mg $317 在庫あり
100 mg $422 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 36 publication(s) in Google Scholar

Other Forms of Epirubicin hydrochloride:

エピルビシン 塩酸塩 関連抗体

Top Publications Citing Use of Products

顧客検証

Cell Proliferation/Viability Assay
In Vivo Efficacy Study
WB

    Epirubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Jan;20(1):51-64.  [Abstract]

    Comparison of drug (Epirubicin hydrochloride, EPI; Daunorubicin hydrochloride, DNR; Vinorelbine ditartrate, VNR; Oxaliplatin, OXA; Vincristine, VCR; Artemisinin, ART; Colchicine, COL) cytotoxicity to TC1 cells at the indicated doses and time points measured with CCK-8 assays. R.U. (Relative unit) was calculated from the average O.D. values in each condition as the indicator of cell viability (n = 3).

    Epirubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2022 Apr 7;82(7):1297-1312.e8.  [Abstract]

    CCK8 assay to detect the survival of MCF7 cells transfected with indicated siRNAs and treated with 1 μM EPI (Epirubicin hydrochloride; for 24 h).

    Epirubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2022 Apr 7;82(7):1297-1312.e8.  [Abstract]

    CCK8 assay to detect the survival of MCF7 cells transfected with indicated siRNAs and treated with 1 μM EPI (Epirubicin hydrochloride; left) for 24 h or 0.5 μM EPI for 0-60 h (right).

    Epirubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2022 Apr 7;82(7):1297-1312.e8.  [Abstract]

    Indicated MCF7 cells were treated with 1 μM EPI (Epirubicin hydrochloride) for 24 h. The levels of γH2AX were analyzed by immunoblotting (IB). Numbers represent its relative intensities measured by ImageJ.

    Epirubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2022 Apr 7;82(7):1297-1312.e8.  [Abstract]

    Xenograft assay of lnc15.2-depleted and FL/FL∗-supplemented MDA-MB-231 cells (n = 5 per group). Tumor growth are shown. The arrow indicates each EPI (Epirubicin hydrochloride; 5 mg/Kg; i.p. on days 0, 4, 8 or 9) treatment.

    Epirubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Asia Pac J Clin Oncol. 2023 Jun;19(3):355-364.  [Abstract]

    The Epirubicin (EPI) + iodine-125 (125I) group is significantly decreases the proliferation ability of HepG2 and SMMC7721 cells (cells are cultured for 2-3 weeks).

    Epirubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Asia Pac J Clin Oncol. 2023 Jun;19(3):355-364.  [Abstract]

    Epirubicin (EPI) promotes iodine-125 (125I)-induced downregulation of the WNT pathway and enhances the radiosensitivity of HepG2 and SMMC7721 cells.

    DNA/RNA Synthesis アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

    Topoisomerase アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Topoisomerase Topo I Topo II DNA gyrase

    Antibiotic アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

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    製品説明

    Epirubicin hydrochloride (4'-Epidoxorubicin hydrochloride), a semisynthetic L-arabino derivative of doxorubicin, has an antineoplastic agent by inhibiting Topoisomerase[1]. Epirubicin hydrochloride inhibits DNA and RNA synthesis. Epirubicin hydrochloride is a Forkhead box protein p3 (Foxp3) inhibitor and inhibits regulatory T cell activity[2].

    IC50 & Target[1]

    Topoisomerase

     

    体外実験

    Epirubicin hydrochloride (4'-Epidoxorubicin hydrochloride), like doxorubicin, exerts its antitumor effects by complex with DNA, resulting in damage to DNA and interference with the synthesis of DNA, RNA, and proteins. Epirubicin hydrochloride may also affect the integrity and activity of cellular membranes. Maximal cell kill caused by Epirubicin hydrochloride occurs during the S phase of the cell cycle. With higher concentrations effects are also seen in early G2 as well as G1 and M phases[1].
    Epirubicin hydrochloride display antineoplastic activity against most cancer cells. Epirubicin hydrochloride is cytotoxic to Hepatoma G2 cells with IC50 of 1.6 μg/mL at 24 hr. 1.6 μg/mL Epirubicin hydrochloride induces apoptosis of Hep G2 cells, and higher activity of catalase by 50%, Se-dependent glutathione peroxidase by 110%, Cu, Zn-superoxide dismutase by 172% and Mn-superoxide dismutase by 135%. Epirubicin hydrochloride increases the cellular expression of NADPH-CYP 450 reductase, and reduces GST-π expression[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    エピルビシン 塩酸塩 関連抗体
    体内実験

    Epirubicin hydrochloride (4'-Epidoxorubicin hydrochloride) are clinically active against a broad range of tumor types, including breast cancer, malignant lymphomas, soft tissue sarcomas, lung cancer, pleural mesothelioma, gastrointestinal cancer, head and neck cancer, ovarian cancer, prostatic carcinoma, transitional bladder carcinoma and so on[4].
    Epirubicin hydrochloride at a dose of 3.5 mg/kg suppresses tumor mass of human breast tumor xenograft R-27 by 74.4 %[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    579.98

    分子式

    C27H30ClNO11
    CAS 番号
    Appearance

    Solid

    Color

    Orange to red

    SMILES

    O=C(C1=C2C(O)=C3[C@@H](O[C@@]4([H])C[C@H](N)[C@@H](O)[C@H](C)O4)C[C@@](C(CO)=O)(O)CC3=C1O)C5=CC=CC(OC)=C5C2=O.Cl
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
    溶剤 & 溶解度
    体外: 

    H2O : 50 mg/mL (86.21 mM; Need ultrasonic)

    DMSO : 25 mg/mL (43.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7242 mL 8.6210 mL 17.2420 mL
    5 mM 0.3448 mL 1.7242 mL 3.4484 mL
    10 mM 0.1724 mL 0.8621 mL 1.7242 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

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    体積 （開始）

    V1

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    濃度 （終了）

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    体積 （終了）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  5% DMSO    40% PEG300    5% Tween-80    50% Saline

      Solubility: ≥ 2.5 mg/mL (4.31 mM); Clear solution

    • Protocol 2

      Add each solvent one by one:  5% DMSO    95% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (4.31 mM); Clear solution

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 1.1 mg/mL (1.90 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.62%

    COA (261 KB) HNMR (305 KB) RP-HPLC (246 KB) MS (71 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [3]

    Hep G2 cells (500 cells/well, monolayer) are plated in a 96-well plate. The next day the cells are treated with Epirubicin in the medium. At the end of the incubation periods, 15% volume of MTT dye solution is added. After 1 hr of incubation at 37°C, an equal volume of solubilization/stop solution (dimethylsul-foxide) is added to each well for an additional 1 hr incubation. The absorbance of the reaction solution at 570 nm is recorded.

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 1.7242 mL 8.6210 mL 17.2420 mL 43.1049 mL
    5 mM 0.3448 mL 1.7242 mL 3.4484 mL 8.6210 mL
    10 mM 0.1724 mL 0.8621 mL 1.7242 mL 4.3105 mL
    15 mM 0.1149 mL 0.5747 mL 1.1495 mL 2.8737 mL
    20 mM 0.0862 mL 0.4310 mL 0.8621 mL 2.1552 mL
    25 mM 0.0690 mL 0.3448 mL 0.6897 mL 1.7242 mL
    30 mM 0.0575 mL 0.2874 mL 0.5747 mL 1.4368 mL
    40 mM 0.0431 mL 0.2155 mL 0.4310 mL 1.0776 mL
    H2O 50 mM 0.0345 mL 0.1724 mL 0.3448 mL 0.8621 mL
    60 mM 0.0287 mL 0.1437 mL 0.2874 mL 0.7184 mL
    80 mM 0.0216 mL 0.1078 mL 0.2155 mL 0.5388 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Epirubicin hydrochloride Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Epirubicin56390-09-14'-EpidoxorubicinDNA/RNA SynthesisTopoisomeraseApoptosisAntibioticL-arabinoderivativeFoxp3TcellInhibitorinhibitorinhibit

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