Withangulatin A 

Withangulatin A is the inhibitor for COX-2. Withangulatin A inhibits MAPK, NF-κB, Akt/mTOR/p70S6K pathway, exhibits antitumor, anti-inflammatory and trypanocidal activities^[1][2][3].

Withangulatin A (1.4-5.6 µM, 48 h) inhibits the proliferation of mouse T lymphocyte with an EC₅₀ of 2.89 µM^[1].
Withangulatin A (1.4-5.6 µM, 48 h) reduces LPS (HY-D1056)-induced secretion of IL-2, IFN-γ, and IL-6, suppresses the expression of COX-2 and PGE2 in mouse T lymphocyte^[1].
Withangulatin A (0-20 µM, 24 h) arrests the cell cycle at S phase, induces apoptosis inLX-2 cell^[2].
Withangulatin A (0-20 µM, 24 h) inhibits TGF-β-stimulated collagen I gene expression, inhibits the phosphorylation and nuclear translocation of Smad2/3 in rat primary hepatic stellate cells and LX-2 cell^[2].
Withangulatin A (0-10 µM, 12 h) binds to peroxiredoxin 6 (PRDX6), inhibits the activities of glutathione peroxidase (GPx) and phospholipase A2 (iPLA2), promotes the generation of ROS^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Withangulatin A (5-20 mg/kg, ip, once daily for 5 days) exhibits anti-inflammatory activity in mouse xylene-induced ear swelling models^[1].
Withangulatin A (5-10 mg/kg, ip, 3 times a week for 4 weeks) exhibits antitumor effect in mouse H1975 xenograft models^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

526.62

C₃₀H₃₈O₈

120824-03-5

Solid

White to off-white

C[C@]12[C@@]3([C@H](C=CC2=O)O)[C@](C[C@]4([H])[C@]1([H])CC[C@]5([C@]4([C@H](C=C5[C@@H]([C@@]6([H])CC(C)=C(C(O6)=O)C)C)OC(C)=O)O)C)([H])O3

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Lijuan Sun, et al. Anti-inflammatory function of Withangulatin A by targeted inhibiting COX-2 expression via MAPK and NF-kappaB pathways. J Cell Biochem. 2010 Feb 15;109(3):532-41.  [Content Brief]

  [2]. Liu Q, et al., Withagulatin A inhibits hepatic stellate cell viability and procollagen I production through Akt and Smad signaling pathways. Acta Pharmacol Sin. 2010 Aug;31(8):944-52.  [Content Brief]

  [3]. Chen C, et al., Identification of peroxiredoxin 6 as a direct target of withangulatin A by quantitative chemical proteomics in non-small cell lung cancer. Redox Biol. 2021 Oct;46:102130.  [Content Brief]