Anandamide (Synonyms: AEA; Arachidonoyl ethanolamide)

Name: Anandamide
Brand: MedChemExpress
SKU: HY-10863
Rating: 5.0 (2 reviews)

Cat. No.: HY-10863 Pureza: 98.43%: Ficha de datos
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Anandamide is an endocannabinoid. Anandamide modulates both neuronal and immune functions through two protein-coupled cannabinoid receptors (CB1 and CB2). Anandamide can activate numerous other receptors like PPARS, TRPV1, and GPR18/GPR55. Anandamide also has potential anti-fungal and anti-inflammatory activities. Anandamide can be used for the research of Alzheimer’s disease (AD) and ulcerative colitis.

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No. CAS : 94421-68-8

Tamaño	Stock	Cantidad
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Liquid
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

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Revisión del cliente

Based on 2 publication(s) in Google Scholar

Other Forms of Anandamide:

Anandamide-d₈ In-stock
Anandamide-d₄ In-stock
Anandamide-d₁₁ Obtener un presupuesto

2 Publications Citing Use of MCE Anandamide

Cell Proliferation/Viability Assay

Bio/Physico-chemical Assay

: Anandamide purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jun 13;23(1):280. [Abstract]; Huh7 cells were treated with oxaliplatin (2 µM), oxaliplatin combined with Anandamide (Arachidonic acid; AA) (10 µM) for the indicated days. Anandamide (Arachidonic acid; AA) promoted the anti-tumor effect of oxaliplatin in a time-dependent manner (0–4 day).

: Anandamide purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jun 13;23(1):280. [Abstract]; Huh7 cells were pretreated with Anandamide (Arachidonic acid; AA) (10 µM; 2 h) and then exposed to Yoda1 (10 µM; 30 min). Intracellular calcium influx was observed using a Fluo-4 Calcium Assay Kit.

: Anandamide purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jun 13;23(1):280. [Abstract]; Huh7 cells were treated with oxaliplatin (2 µM) or oxaliplatin combined with Anandamide (Arachidonic acid; AA) (10 µM) for 3 days. Cell viability was detected by a CCK-8 assay.

: Anandamide purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2021 Sep;297(3):101022. [Abstract]; Representative whole-cell patch-clamp recording showed that Anandamide (AEA) (0.01–100 μM) activate the TRPV1 in a concentration-dependent manner.

: Anandamide purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2021 Sep;297(3):101022. [Abstract]; Anandamide (AEA) (0.01–100 μM) activated the TRPV1 channel in a concentration-dependent manner in HEK293 cells.

Recomendaciones destacadas

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas Cannabinoid Receptor:

Ver todas las isoformas

CB1 CB2

Ver todos los productos específicos de isoformas PPAR:

Ver todas las isoformas

PPARα PPARβ/δ PPARγ PPAR PPARδ

Ver todos los productos específicos de isoformas TRP Channel:

Ver todas las isoformas

TRPA1 TRPC3 TRPC4 TRPC5 TRPC6 TRPC7 TRPM8 TRPM2 TRPM3 TRPM4 TRPM7 TRPML1 TRPML2 TRPML3 TRPV1 TRPV2 TRPV3 TRPV4 TRPV6

Ver todos los productos específicos de isoformas Endogenous Metabolite:

Ver todas las isoformas

Human Endogenous Metabolite Microbial Metabolite

Actividad biológica
Pureza y Documentación
Referencias
Revisión del cliente

Descripciòn

IC₅₀ & Target^[1]

CB1

CB2

PPAR

TRPV1

Microbial Metabolite

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
RBL-2H3	IC₅₀	> 50 3 Compound: 14	Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity preincubated for 30 mins followed by DNP-HSA stimulation and measured after 30 mins by 4-nitrophenyl 2 Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity preincubated for 30 mins followed by DNP-HSA stimulation and measured after 30 mins by 4-nitrophenyl 2	[PMID: 31618024]
HEK293	EC₅₀	0.2 3 Compound: AEA	Agonist activity at human recombinant TRPV1 expressed in HEK293 cells assessed as increase in intracellular calcium level Agonist activity at human recombinant TRPV1 expressed in HEK293 cells assessed as increase in intracellular calcium level	[PMID: 19361197]
HEK293	EC₅₀	0.2 3 Compound: AEA	Agonist activity at human recombinant TRPV1 expressed in HEK293 cells assessed as increase in intracellular calcium level Agonist activity at human recombinant TRPV1 expressed in HEK293 cells assessed as increase in intracellular calcium level	[PMID: 19361197]
HEK293	EC₅₀	0.49 3 Compound: 1a, AEA	Agonist activity on human recombinant TRPV1-mediated enhancement of intracellular calcium concentration in HEK293 cells Agonist activity on human recombinant TRPV1-mediated enhancement of intracellular calcium concentration in HEK293 cells	[PMID: 16570929]
HEK293	EC₅₀	0.49 3 Compound: 1a, AEA	Agonist activity on human recombinant TRPV1-mediated enhancement of intracellular calcium concentration in HEK293 cells Agonist activity on human recombinant TRPV1-mediated enhancement of intracellular calcium concentration in HEK293 cells	[PMID: 16570929]
HEK293	EC₅₀	630 1 Compound: 21, AEA	Activation of human VR1 expressed in HEK293 cells assessed as stimulation of Ca2+ influx Activation of human VR1 expressed in HEK293 cells assessed as stimulation of Ca2+ influx	[PMID: 23865723]
HEK293	EC₅₀	630 1 Compound: 21, AEA	Activation of human VR1 expressed in HEK293 cells assessed as stimulation of Ca2+ influx Activation of human VR1 expressed in HEK293 cells assessed as stimulation of Ca2+ influx	[PMID: 23865723]
RBL-2H3	IC₅₀	>50 3 Compound: 14	Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity preincubated for 30 mins followed by DNP-HSA stimulation and measured after 30 mins by 4-nitrophenyl 2 Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity preincubated for 30 mins followed by DNP-HSA stimulation and measured after 30 mins by 4-nitrophenyl 2	[PMID: 31618024]
RBL-2H3	IC₅₀	14.5 3 Compound: 1a, AEA	Inhibition of AEA uptake in RBL2H3 cells Inhibition of AEA uptake in RBL2H3 cells	[PMID: 16570929]
RBL-2H3	IC₅₀	14.5 3 Compound: 1a, AEA	Inhibition of AEA uptake in RBL2H3 cells Inhibition of AEA uptake in RBL2H3 cells	[PMID: 16570929]
RBL-2H3	IC₅₀	> 50 3 Compound: 14	Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity preincubated for 30 mins followed by DNP-HSA stimulation and measured after 30 mins by 4-nitrophenyl 2 Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity preincubated for 30 mins followed by DNP-HSA stimulation and measured after 30 mins by 4-nitrophenyl 2	[PMID: 31618024]

In Vitro

Anandamide (0-250 μg/ml, 1 h) inhibits C. albicans hyphal growth and prevents hyphal adherence to epithelial cells^[4].
Anandamide (0-250 μg/ml, 2 h) alters the expression of genes involved in adhesion and hyphal morphogenesis^[4].
Anandamide reduces tau phosphorylation through the inhibition of the activity of protein kinases^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[4]

Cell Line:	HeLa cervical epithelial cells
Concentration:	0, 10, 50, 125, 250 μg/ml
Incubation Time:	2 h
Result:	Repressed the expression of the HWP1 and ALS3 adhesins involved in Candida adhesion to epithelial cells and the HGC1, RAS1, EFG1 and ZAP1 regulators of hyphal morphogenesis and cell adherence. Increased the expression of NRG1, a transcriptional repressor of filamentous growth.

In Vivo

Anandamide (10 mg/kg, IP, once) considerably lowers the increase of glycemia in response to glucose ingestion compared with control, and this is associated with an improvement of glucose tolerance^[2].
Anandamide (100 ng, ICV bilateral injection, single) partially prevents streptozotocin (STZ)-induced cognitive impairments, changes in synaptic markers and ventricle enlargement^[3].
Anandamide exerts anti-inflammatory activities, attenuating the development of inflammation in a mouse model of ulcerative colitis^[4].
Anandamide alleviates lipopolysaccharide (LPS)-induced neuroinflammation in rat primary microglial cultures^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Wistar rats (3-4 months, 350-400 g, STZ-induced AD-like sporadic dementia model)^[3]
Dosage:	100 ng
Administration:	ICV bilateral injection, single
Result:	Prevented recognition and non-associative emotional memory impairments induced by STZ, but did not influence tone fear conditioning. Decreased BAG2 (Bcl2-associated athanogene) levels, and prevented STZ-induced ventricle enlargement.

Peso molecular

347.53

Fòrmula

C₂₂H₃₇NO₂

No. CAS

94421-68-8

Appearance

Liquid (Density: 0.940±0.06 g/cm³)

Color

Yellow to brown

SMILES

CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(NCCO)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (287.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8774 mL	14.3872 mL	28.7745 mL
5 mM	0.5755 mL	2.8774 mL	5.7549 mL
10 mM	0.2877 mL	1.4387 mL	2.8774 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: ≥99.0%

Select Batch:

Ficha de datos (280 KB) SDS (477 KB)

COA (249 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Malek N, et al. Anandamide, Acting via CB2 Receptors, Alleviates LPS-Induced Neuroinflammation in Rat Primary Microglial Cultures. Neural Plast. 2015;2015:130639. [Content Brief]

[2]. Troy-Fioramonti S, et al. Acute activation of cannabinoid receptors by Anandamide reduces gastrointestinal motility and improves postprandial glycemia in mice. Diabetes. 2015 Mar;64(3):808-18. [Content Brief]

[3]. Moreira-Silva D, et al. Anandamide Effects in a Streptozotocin-Induced Alzheimer's Disease-Like Sporadic Dementia in Rats. Front Neurosci. 2018 Sep 21;12:653. [Content Brief]

[4]. Sionov RV, et al. Anandamide prevents the adhesion of filamentous Candida albicans to cervical epithelial cells. Sci Rep. 2020 Aug 13;10(1):13728. [Content Brief]

[5]. Pflüger-Müller B, et al. The endocannabinoid anandamide has an anti-inflammatory effect on CCL2 expression in vascular smooth muscle cells. Basic Res Cardiol. 2020 Apr 22;115(3):34. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8774 mL	14.3872 mL	28.7745 mL	71.9362 mL
	5 mM	0.5755 mL	2.8774 mL	5.7549 mL	14.3872 mL
	10 mM	0.2877 mL	1.4387 mL	2.8774 mL	7.1936 mL
	15 mM	0.1918 mL	0.9591 mL	1.9183 mL	4.7957 mL
	20 mM	0.1439 mL	0.7194 mL	1.4387 mL	3.5968 mL
	25 mM	0.1151 mL	0.5755 mL	1.1510 mL	2.8774 mL
	30 mM	0.0959 mL	0.4796 mL	0.9591 mL	2.3979 mL
	40 mM	0.0719 mL	0.3597 mL	0.7194 mL	1.7984 mL
	50 mM	0.0575 mL	0.2877 mL	0.5755 mL	1.4387 mL
	60 mM	0.0480 mL	0.2398 mL	0.4796 mL	1.1989 mL
	80 mM	0.0360 mL	0.1798 mL	0.3597 mL	0.8992 mL
	100 mM	0.0288 mL	0.1439 mL	0.2877 mL	0.7194 mL

Anandamide Related Classifications

Neurological Disease Metabolic Disease Inflammation/Immunology Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Anandamide (Synonyms: AEA; Arachidonoyl ethanolamide)

Revisión del cliente

Other Forms of Anandamide:

Anandamide Related Antibodies

2 Publications Citing Use of MCE Anandamide

Recomendaciones destacadas

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas Cannabinoid Receptor:

Ver todos los productos específicos de isoformas PPAR:

Ver todos los productos específicos de isoformas TRP Channel:

Ver todos los productos específicos de isoformas Endogenous Metabolite:

Actividad biológica

Pureza y Documentación

Referencias

Revisión del cliente

Anandamide Related Antibodies

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

Anandamide Related Classifications

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Productos vistos recientemente:

Revisión del cliente

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