2. Membrane Transporter/Ion Channel Immunology/Inflammation Anti-infection Apoptosis Stem Cell/Wnt
  3. Potassium Channel NOD-like Receptor (NLR) Bacterial Antibiotic Pyroptosis Wnt β-catenin
  4. Nigericin sodium salt

Nigericin Sodium Salt  (Synonyms: ニゲリシンナトリウム)

製品番号: HY-100381 純度: 99.60%
COA 取扱説明書 Technical Support

Nigericin sodium salt is an antibiotic derived from Streptomyces hygroscopicus that act as a K+/H+ ionophore, promoting K+/H+ exchange across mitochondrial membranes. Nigericin sodium salt is a NLRP3 activator. Nigericin sodium salt shows promising anti-cancer activities through decreasing intracellular pH (pHi), and inactivation of Wnt/β-catenin signals. Nigericin sodium salt induces pyroptosis through caspase 1/GSDMD in TNBC.

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CAS 番号 : 28643-80-3

容量 価格（税別） 在庫状況 数量
Solution
10 mM * 1 mL in Ethanol USD 148 在庫あり
Solid
5 mg $90 在庫あり
10 mg $150 在庫あり
25 mg $218 在庫あり
50 mg $290 在庫あり
100 mg $450 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 221 publication(s) in Google Scholar

Other Forms of Nigericin sodium salt:

Nigericin Sodium Salt 関連抗体

Top Publications Citing Use of Products

顧客検証

WB

    Nigericin sodium salt purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Dec 30;9(1):367.  [Abstract]

    Cellular BiP-IRF2BP2 interactions shown by Western blot analysis of co-immunoprecipitation in MG132-pretreated (5 μM, 2 h) peritoneal macrophages which were incubated with Nigericin (10 μM), Valinomycin (10 μM) and MG132 (5 μM) for 3 h in calibration buffer under different pH (7.2, 7.0, 6.8, 6.6).

    Nigericin sodium salt purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 May 28:131:155758.  [Abstract]

    Nigericin (10 μM; 30 min) inhibits caspase-1 cleavage and IL-1β secretion in BMDMs.

    Nigericin sodium salt purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Jul 12:177:117121.  [Abstract]

    Nigericin (10 μM; 24 h) significantly upregulated the protein expression of NLRP3, Caspase-1, ASC, IL-1β, and IL-18 in hypoxia-treated H9c2 cells, reversing the inhibitory effects of Celastrol.

    Nigericin sodium salt purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 May:113:154743.  [Abstract]

    Priming with LPS (1 μg/mL; 4 h) and activating with Nigericin (10 μM; 2 h) induces NLRP3 expression, caspase-1 cleavage, and GSDMD cleavage in BMDMs.

    Nigericin sodium salt purchased from MedChemExpress. Usage Cited in: Environ Toxicol. 2023 Jun;38(6):1405-1419.  [Abstract]

    Nigericin (1.38mM; 2 μg/2 μL; 10 min) signifcantly increases the expression of IL-1β, IL-18, and GSDMD N-terminal in rats

    Nigericin sodium salt purchased from MedChemExpress. Usage Cited in: Eur J Med Chem. 2023 Jan 5;245(Pt 2):114919.  [Abstract]

    Nigericin (10 μM; 0.5 h) combined with LPS (1 μg/mL; 4.5 h) markedly increases the expression of cleaved caspase-1 and IL-1β in the culture medium of THP-M cells.

    Nigericin sodium salt purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2020 Jul;24(14):8078-8090.  [Abstract]

    LPS + Nigericin is used to induce pyroptosis as a positive control group.

    NOD-like Receptor (NLR) アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    NLRP3 NLRP1 NOD1 NOD2

    Antibiotic アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

    Wnt アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Wnt1 Wnt3 Wnt5 Wnt7 Wnt11

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    • 純度とドキュメンテーション

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    製品説明

    Nigericin sodium salt is an antibiotic derived from Streptomyces hygroscopicus that act as a K+/H+ ionophore, promoting K+/H+ exchange across mitochondrial membranes. Nigericin sodium salt is a NLRP3 activator. Nigericin sodium salt shows promising anti-cancer activities through decreasing intracellular pH (pHi), and inactivation of Wnt/β-catenin signals. Nigericin sodium salt induces pyroptosis through caspase 1/GSDMD in TNBC[1][2][3][4][5][6][7].

    IC50 & Target

    NLRP3

     

    体外実験

    Nigericin (0-4 μg/mL; 24 h) sodium salt induces pyroptosis through caspase 1/GSDMD pathway in TNBC cells[6].
    Nigericin (1 μg/mL, 8 × MIC; 2 μg/mL, 16 × MIC; 196 h) sodium salt is a bactericidal antibiotic against MDR gram-positive bacteria[4].
    Nigericin (0-0.25 μg/mL; 24 h) sodium salt inactivates Wnt/β-catenin pathway in H460 cells as an anti-cancer effect[7].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Nigericin Sodium Salt 関連抗体

    Cell Viability Assay[4]

    Cell Line: S. aureus, Staphylococcus epidermidis, Enterococcus faecalis, E. faecium, S. pneumoniae) , and Streptococcus agalactiae
    Concentration: 0 μg/mL, 0.05 μg/mL, 0.125 μg/mL, 0.25 μg/mL
    Incubation Time: 24 h
    Result: Exhibited potent activity against these clinical MDR strains, with MIC values ranging from 0.004-0.25 mg/mL.

    Western Blot Analysis[6]

    Cell Line: MDA-MB-231, and 4T1 cells
    Concentration: 0 μg/mL, 2 μg/mL, 4 μg/mL
    Incubation Time: 24 h
    Result: Showed increasing in the proteins level of caspase 1 and N-GSDMD.
    体内実験

    Nigericin (1 mg/kg; i.p.; every 12 h for 3 D) sodium salt reduces the infection of S. aureus USA300 in mice[4].
    Nigericin (0.025 mg/kg; s.c.; every two days in 4 weeks) sodium salt plus anti-PD-1 shows synergistic anti-cancer effect[6].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Mice Infected with S. aureus USA300[4]
    Dosage: 1 mg/kg
    Administration: Intraperitoneal injection (i.p.)
    Result: Showed reduction in the bacterial burden to 1,000-10,000-fold in the major organs.
    Animal Model: BALB/c Mice injected orthotopically with 4T1 cells[6]
    Dosage: 0.025 mg/kg
    Administration: Subcutaneous injection (s.c.)
    Result: Showed combination with anti-PD-1 antibody almost completely suppressed tumor growth.
    分子量

    746.94

    分子式

    C40H67NaO11
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@H]1[C@](O[C@@H]2C[C@](CC[C@@H]3C)([H])O[C@@]3([H])[C@@H](C)C(O[Na])=O)([C@@H]([C@H](OC)C2)C)O[C@](C)([C@@]4([H])O[C@@](C)([C@@]5([H])O[C@@]([C@]([C@@H](C[C@@H]6C)C)([H])O[C@]6(O)CO)([H])C[C@H]5C)CC4)C1
    Structure Classification
    Initial Source

    Streptomyces hygroscopicus
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    Ethanol : 25 mg/mL (33.47 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.3388 mL 6.6940 mL 13.3880 mL
    5 mM 0.2678 mL 1.3388 mL 2.6776 mL
    10 mM 0.1339 mL 0.6694 mL 1.3388 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

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    体積 （終了）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (3.35 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% EtOH    90% (20% SBE-β-CD in Saline)

      Solubility: 2.5 mg/mL (3.35 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    純度とドキュメンテーション

    純度: 99.60%

    COA (251 KB) HNMR (326 KB) RP-HPLC (212 KB) LCMS (92 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol 1 mM 1.3388 mL 6.6940 mL 13.3880 mL 33.4699 mL
    5 mM 0.2678 mL 1.3388 mL 2.6776 mL 6.6940 mL
    10 mM 0.1339 mL 0.6694 mL 1.3388 mL 3.3470 mL
    15 mM 0.0893 mL 0.4463 mL 0.8925 mL 2.2313 mL
    20 mM 0.0669 mL 0.3347 mL 0.6694 mL 1.6735 mL
    25 mM 0.0536 mL 0.2678 mL 0.5355 mL 1.3388 mL
    30 mM 0.0446 mL 0.2231 mL 0.4463 mL 1.1157 mL
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    Nigericin sodium salt Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Nigericin28643-80-3Potassium ChannelNOD-like Receptor (NLR)BacterialAntibioticPyroptosisWntβ-cateninKcsABeta cateninInhibitorinhibitorinhibit

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    製品番号:
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