2. Metabolic Enzyme/Protease MAPK/ERK Pathway PI3K/Akt/mTOR NF-κB
  3. Endogenous Metabolite p38 MAPK Akt Keap1-Nrf2 NF-κB
  4. Ergothioneine

エルゴチオネイン  (Synonyms: Ergothioneine; L-(+)-Ergothioneine)

製品番号: HY-N1914 純度: 99.98%
COA 取扱説明書 Technical Support

Ergothioneine is an imidazole-2-thione derivative with orally active histidine betaine. Ergothioneine is a specific inhibitor of p38-MAPK and Akt, which plays a protective role in cell apoptosis induced by stress. Ergothioneine has antioxidant activity.

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CAS 番号 : 497-30-3

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 67 在庫あり
Solution
10 mM * 1 mL in Water USD 67 在庫あり
Solid
5 mg $61 在庫あり
10 mg $95 在庫あり
25 mg $190 在庫あり
50 mg $365 在庫あり
100 mg $511 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 13 publication(s) in Google Scholar

Other Forms of Ergothioneine:

エルゴチオネイン 関連抗体

Top Publications Citing Use of Products

    Ergothioneine purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2024 Jun 12;16(23):29917-29929.  [Abstract]

    Ergothioneine (EGT; 40 μg/mL; 48h). Fluorescence images of live/dead staining.

    Ergothioneine purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2024 Jun 12;16(23):29917-29929.  [Abstract]

    Ergothioneine (EGT; 40 μg/mL; 0-6 days). Survival fraction at different radiation dosages by colony formation assay.

    Ergothioneine purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2024 Jun 12;16(23):29917-29929.  [Abstract]

    Ergothioneine (EGT; 40 μg/mL; 24 h). Analysis of DCF fluorescence by flow cytometry.

    Ergothioneine purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2024 Jun 12;16(23):29917-29929.  [Abstract]

    Ergothioneine (EGT; 40 μg/mL; 24 h). Statistics of apoptosis rate.

    Ergothioneine purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2024 Jun 12;16(23):29917-29929.  [Abstract]

    Ergothioneine (EGT; 40 μg/mL; 24 h). Expression of Bcl-2, Bax, c-Caspase-3, and c-PARP following varying treatments.

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

    p38 MAPK アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

    Akt アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Akt Akt1 Akt2 Akt3

    NF-κB アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Ergothioneine is an imidazole-2-thione derivative with orally active histidine betaine. Ergothioneine is a specific inhibitor of p38-MAPK and Akt, which plays a protective role in cell apoptosis induced by stress. Ergothioneine has antioxidant activity[1][2].

    IC50 & Target

    Microbial Metabolite

     

    Human Endogenous Metabolite

     

    Cellular Effect
    Cell Line Type Value Description References
    HEK293 EC50
    40 μM
    Compound: 5351619
    Substrate uptake by the Organic Cation/Carnitine Transporter 1 (OCTN1, SLC22A4) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 cells stably transfected with SLC22A4 (PubChem AID: 1794816)
    Substrate uptake by the Organic Cation/Carnitine Transporter 1 (OCTN1, SLC22A4) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 cells stably transfected with SLC22A4 (PubChem AID: 1794816)
    		10.5281/zenodo.7360363
    体外実験

    Ergothioneine (0.25, 1 mM, 23 h) can regulate PC12 DNA damage, MAPKs activation and cell death induced by hydrogen peroxide[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    エルゴチオネイン 関連抗体

    Cell Viability Assay [1]

    Cell Line: PC12
    Concentration: 0.25, 1 mM
    Incubation Time: 23 h
    Result: Increased cell viability by approximately 13% and 34%, respectively.

    Western Blot Analysis [1]

    Cell Line: PC12
    Concentration: 0.25, 1 mM
    Incubation Time: 23 h
    Result: Counteracted the p38 phosphorylation induced by 1 h incubation with H2O2.
    体内実験

    Ergothioneine (70 mg/kg orally, For 14 consecutive days) mediates the improvement of cisplatin-induced nephrotoxicity in rats by regulating Nrf2, p53 and NF-κB signaling and inhibiting γ-glutamyltranspeptidase[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Cisplatin-treated rats[1]
    Dosage: 70 mg/kg
    Administration: p.o. For 14 consecutive days
    Result: Decreased serum creatinine and BUN.
    Increased GFR at two-fold.
    Suppressed cisplatin-induced oxidative stress and GCT.
    Up-regulated the levels of Nrf2, NQO1, and HO-1.
    Suppresses NF-κB signaling and the pro-inflammatory cytokines.
    臨床実験
    分子量

    229.30

    分子式

    C9H15N3O2S
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    S=C1NC(C[C@@H](C([O-])=O)[N+](C)(C)C)=CN1
    Structure Classification
    Initial Source

    many bacteria and most fungi
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    H2O : 125 mg/mL (545.14 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.3611 mL 21.8055 mL 43.6110 mL
    5 mM 0.8722 mL 4.3611 mL 8.7222 mL
    10 mM 0.4361 mL 2.1805 mL 4.3611 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

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    体積 （終了）

    V2

    体内:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (436.11 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.98%

    COA (249 KB) HNMR (248 KB) RP-HPLC (255 KB) MS (205 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 4.3611 mL 21.8055 mL 43.6110 mL 109.0275 mL
    5 mM 0.8722 mL 4.3611 mL 8.7222 mL 21.8055 mL
    10 mM 0.4361 mL 2.1805 mL 4.3611 mL 10.9027 mL
    15 mM 0.2907 mL 1.4537 mL 2.9074 mL 7.2685 mL
    20 mM 0.2181 mL 1.0903 mL 2.1805 mL 5.4514 mL
    25 mM 0.1744 mL 0.8722 mL 1.7444 mL 4.3611 mL
    30 mM 0.1454 mL 0.7268 mL 1.4537 mL 3.6342 mL
    40 mM 0.1090 mL 0.5451 mL 1.0903 mL 2.7257 mL
    50 mM 0.0872 mL 0.4361 mL 0.8722 mL 2.1805 mL
    60 mM 0.0727 mL 0.3634 mL 0.7268 mL 1.8171 mL
    80 mM 0.0545 mL 0.2726 mL 0.5451 mL 1.3628 mL
    100 mM 0.0436 mL 0.2181 mL 0.4361 mL 1.0903 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Ergothioneine Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Ergothioneine497-30-3L-(+)-ErgothioneineEndogenous Metabolitep38 MAPKAktKeap1-Nrf2NF-κBPKBProtein kinase BNuclear factor-κBNuclear factor-kappaBMAPKAntioxidantPC12CisplatinDNA fragmentationInhibitorinhibitorinhibit

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