2. Others Apoptosis Anti-infection
  3. Parasite Environmental Pollutants Bacterial Antibiotic Apoptosis
  4. Metronidazole

Metronidazole is an orally active nitroimidazole antibiotic. Metronidazole can cross blood brain barrier. Metronidazole can be used for the research of anaerobic infections.

For research use only. We do not sell to patients.

CAS No. : 443-48-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
5 g In-stock
10 g In-stock
25 g In-stock
50 g In-stock
100 g In-stock
> 100 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 65 publication(s) in Google Scholar

Other Forms of Metronidazole:

Metronidazole Related Antibodies

Top Publications Citing Use of Products

65 Publications Citing Use of MCE Metronidazole

WB
ELISA
In Vivo Imaging
PK/PD Analysis
Bio/Physico-chemical Assay
RT-PCR

    Metronidazole purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2025 Dec;17(1):2545434.  [Abstract]

    Western blot analysis of METTL3 and YTHDF2 protein expression from the CON and ABX (Ampicillin (1 g/L), Neomycin (1 g/L), Metronidazole (1 g/L), and vancomycin (0.5 g/L); drinking water from 6 to 12 weeks) mice (n = 3/group).

    Metronidazole purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2025 Dec;17(1):2545434.  [Abstract]

    RT-qPCR analysis of METTL3 and YTHDF2 protein expression from the CON and ABX (Ampicillin (1 g/L), Neomycin (1 g/L), Metronidazole (1 g/L), and vancomycin (0.5 g/L); drinking water from 6 to 12 weeks) mice (n = 3/group).

    Metronidazole purchased from MedChemExpress. Usage Cited in: J Exp Med. 2026 Mar 2;223(3):e20241287.  [Abstract]

    The number and frequency of MAIT cells in the ear pinnae and lungs of mice at 8 wk old following treatment with Metronidazole (30 mg/kg/day; oral gavage; from 2 to 4 weeks).

    Metronidazole purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2025 Nov 4;22(1):259.  [Abstract]

    Sufficiently enhanced susceptibility to JE by ampicillin (A) and/or Metronidazole (M) (1 g/L; drinking water; for 21 days) treatment in C57BL/6 mice.

    Metronidazole purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2023 Dec;15(2):2249143.  [Abstract]

    Targeted metabolomics analysis of gut microbial metabolites (AA, PA, and BA) in colon contents (feces) and serum from mice with or without ABX treatment (n = 6 per group). ABX: an antibiotic cocktail of 0.5 g/L Vancomycin, 1 g/L Metronidazole,1 g/L Neomycin and 1 g/L Ampicillin

    Metronidazole purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2023 Dec;15(2):2282790.  [Abstract]

    Depletion of CD8+ T cells in ABX-treated B16-F0 tumor-bearing mice dampened the antitumor effect of butyrate combined with anti-PD-1 (Figure 4b–e). Decreased serum IFN-γ and TNF-α production during the depletion of CD8+ T cells was also observed. ABX：an antibiotic cocktail of 0.5 g/L Vancomycin, 1 g/L Metronidazole, 1 g/L Neomycin and 1 g/L Ampicillin.

    View All Parasite Isoform Specific Products:

    View All Isoforms
    Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

    View All Antibiotic Isoform Specific Products:

    View All Isoforms
    Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Metronidazole is an orally active nitroimidazole antibiotic. Metronidazole can cross blood brain barrier. Metronidazole can be used for the research of anaerobic infections[1][2][3][4].

    Cellular Effect
    Cell Line Type Value Description References
    A549 IC50
    223.78 6
    Compound: MTZ
    Cytotoxicity against human A549 cells after 24 hrs by MTT assay
    Cytotoxicity against human A549 cells after 24 hrs by MTT assay
    		[PMID: 30554970]
    A549 IC50
    223.78 6
    Compound: MTZ
    Cytotoxicity against human A549 cells after 24 hrs by MTT assay
    Cytotoxicity against human A549 cells after 24 hrs by MTT assay
    		[PMID: 30554970]
    A549 IC50
    > 100 3
    Compound: Mtz
    Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    		[PMID: 27598233]
    A549 IC50
    >100 3
    Compound: Mtz
    Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    		[PMID: 27598233]
    BEAS-2B IC50
    2.24 2
    Compound: MTZ
    Cytotoxicity against human BEAS-2B cells assessed as reduction in cell viability after 24 hrs by MTT assay
    Cytotoxicity against human BEAS-2B cells assessed as reduction in cell viability after 24 hrs by MTT assay
    		[PMID: 33231455]
    CHO IC50
    >400 3
    Compound: 18; MNZ
    Cytotoxicity against CHO cells assessed as reduction in cell viability after 48 hrs by MTT assay
    Cytotoxicity against CHO cells assessed as reduction in cell viability after 48 hrs by MTT assay
    		[PMID: 27597415]
    CHO IC50
    280 3
    Compound: 18; MNZ
    Cytotoxicity against CHO cells assessed as reduction in cell viability after 72 hrs by MTT assay
    Cytotoxicity against CHO cells assessed as reduction in cell viability after 72 hrs by MTT assay
    		[PMID: 27597415]
    CHO IC50
    177.9 3
    Compound: metronidazole
    Inhibition of Cav1.2 current measured using QPatch automatic path clamp system in CHO cells expressing Cav1.2, beta-2 and alpha-2/delta-1 subunits
    Inhibition of Cav1.2 current measured using QPatch automatic path clamp system in CHO cells expressing Cav1.2, beta-2 and alpha-2/delta-1 subunits
    		[PMID: 23812503]
    CHO-AA8 IC50
    23 2
    Compound: 5
    Concentration required to reduce AA8 cell numbers to 50% of controls in a growth inhibition microassay
    Concentration required to reduce AA8 cell numbers to 50% of controls in a growth inhibition microassay
    		[PMID: 8308864]
    BEAS-2B IC50
    2.24 2
    Compound: MTZ
    Cytotoxicity against human BEAS-2B cells assessed as reduction in cell viability after 24 hrs by MTT assay
    Cytotoxicity against human BEAS-2B cells assessed as reduction in cell viability after 24 hrs by MTT assay
    		[PMID: 33231455]
    CHO IC50
    177.9 3
    Compound: metronidazole
    Inhibition of Cav1.2 current measured using QPatch automatic path clamp system in CHO cells expressing Cav1.2, beta-2 and alpha-2/delta-1 subunits
    Inhibition of Cav1.2 current measured using QPatch automatic path clamp system in CHO cells expressing Cav1.2, beta-2 and alpha-2/delta-1 subunits
    		[PMID: 23812503]
    CHO-K1 IC50
    2.92 2
    Compound: MTZ
    Cytotoxicity against CHO-K1 cells assessed as reduction in cell viability after 24 hrs by MTT assay
    Cytotoxicity against CHO-K1 cells assessed as reduction in cell viability after 24 hrs by MTT assay
    		[PMID: 33231455]
    Epithelial cell IC50
    >100 3
    Compound: Metronidazole
    Cytotoxicity against human kidney epithelial cells after 44 hrs by MTT assay
    Cytotoxicity against human kidney epithelial cells after 44 hrs by MTT assay
    		[PMID: 18068273]
    CHO IC50
    280 3
    Compound: 18; MNZ
    Cytotoxicity against CHO cells assessed as reduction in cell viability after 72 hrs by MTT assay
    Cytotoxicity against CHO cells assessed as reduction in cell viability after 72 hrs by MTT assay
    		[PMID: 27597415]
    Epithelial cell IC50
    >100 3
    Compound: Metronidazole
    Cytotoxicity against human kidney epithelial cells after 44 hrs by MTT assay
    Cytotoxicity against human kidney epithelial cells after 44 hrs by MTT assay
    		[PMID: 18378360]
    CHO IC50
    > 400 3
    Compound: 18; MNZ
    Cytotoxicity against CHO cells assessed as reduction in cell viability after 48 hrs by MTT assay
    Cytotoxicity against CHO cells assessed as reduction in cell viability after 48 hrs by MTT assay
    		[PMID: 27597415]
    Epithelial cell IC50
    >100 3
    Compound: metronidazole
    Cytotoxicity against human kidney epithelial cells after 44 hrs by MTT assay
    Cytotoxicity against human kidney epithelial cells after 44 hrs by MTT assay
    		[PMID: 18187238]
    H9c2 IC50
    >200 3
    Compound: MNZ
    Cytotoxicity against rat H9c2 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against rat H9c2 cells assessed as cell viability after 48 hrs by MTT assay
    		[PMID: 21715066]
    H9c2 IC50
    >116 3
    Compound: Metronidazole
    Toxicity in rat H9c2 cells after 48 hrs by MTT assay
    Toxicity in rat H9c2 cells after 48 hrs by MTT assay
    		[PMID: 19589625]
    HEK293 CC50
    >100 3
    Compound: 1
    Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 24 hrs by resazurin assay
    Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 24 hrs by resazurin assay
    		[PMID: 27236016]
    HEK293 IC50
    >100 3
    Compound: Metronidazole
    Cytotoxicity against HEK cells after 44 hrs by MTT assay
    Cytotoxicity against HEK cells after 44 hrs by MTT assay
    		[PMID: 18384916]
    CHO-AA8 IC50
    23 2
    Compound: 5
    Concentration required to reduce AA8 cell numbers to 50% of controls in a growth inhibition microassay
    Concentration required to reduce AA8 cell numbers to 50% of controls in a growth inhibition microassay
    		[PMID: 8308864]
    HeLa EC50
    >1000 6
    Compound: Metronidazole
    Cytotoxicity against human HeLa cells by MTT assay
    Cytotoxicity against human HeLa cells by MTT assay
    		[PMID: 22846917]
    HeLa IC50
    >1000 6
    Compound: MTZ
    Cytotoxicity against human HeLa cells
    Cytotoxicity against human HeLa cells
    		[PMID: 24900434]
    CHO-K1 IC50
    2.92 2
    Compound: MTZ
    Cytotoxicity against CHO-K1 cells assessed as reduction in cell viability after 24 hrs by MTT assay
    Cytotoxicity against CHO-K1 cells assessed as reduction in cell viability after 24 hrs by MTT assay
    		[PMID: 33231455]
    HeLa IC50
    >1000 6
    Compound: MTZ
    Cytotoxicity against human HeLa cells after 24 hrs by MTT assay
    Cytotoxicity against human HeLa cells after 24 hrs by MTT assay
    		[PMID: 24727243]
    HeLa IC50
    >5 2
    Compound: MTZ
    Cytotoxicity against human HeLa cells after 24 hrs by MTT assay
    Cytotoxicity against human HeLa cells after 24 hrs by MTT assay
    		[PMID: 27643640]
    Epithelial cell IC50
    > 100 3
    Compound: Metronidazole
    Cytotoxicity against human kidney epithelial cells after 44 hrs by MTT assay
    Cytotoxicity against human kidney epithelial cells after 44 hrs by MTT assay
    		[PMID: 18068273]
    HeLa IC50
    >5 2
    Compound: MTZ
    Cytotoxicity against human HeLa cells after 24 hrs by MTT assay
    Cytotoxicity against human HeLa cells after 24 hrs by MTT assay
    		[PMID: 29216562]
    Epithelial cell IC50
    > 100 3
    Compound: Metronidazole
    Cytotoxicity against human kidney epithelial cells after 44 hrs by MTT assay
    Cytotoxicity against human kidney epithelial cells after 44 hrs by MTT assay
    		[PMID: 18378360]
    Epithelial cell IC50
    > 100 3
    Compound: metronidazole
    Cytotoxicity against human kidney epithelial cells after 44 hrs by MTT assay
    Cytotoxicity against human kidney epithelial cells after 44 hrs by MTT assay
    		[PMID: 18187238]
    HepG2 CC50
    >100 3
    Compound: 1
    Cytotoxicity against human HepG2 cells assessed as cell growth inhibition after 24 hrs by resazurin assay
    Cytotoxicity against human HepG2 cells assessed as cell growth inhibition after 24 hrs by resazurin assay
    		[PMID: 27236016]
    HepG2 IC50
    >100 3
    Compound: MNZ
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    		[PMID: 22236470]
    HepG2 IC50
    >100 3
    Compound: MNZ
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    		[PMID: 23644202]
    HepG2 IC50
    >100 3
    Compound: MNZ, Metronidazole
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    		[PMID: 22658085]
    H9c2 IC50
    > 116 3
    Compound: Metronidazole
    Toxicity in rat H9c2 cells after 48 hrs by MTT assay
    Toxicity in rat H9c2 cells after 48 hrs by MTT assay
    		[PMID: 19589625]
    H9c2 IC50
    > 200 3
    Compound: MNZ
    Cytotoxicity against rat H9c2 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against rat H9c2 cells assessed as cell viability after 48 hrs by MTT assay
    		[PMID: 21715066]
    HepG2 IC50
    >100 3
    Compound: MNZ, Metronidazole
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    		[PMID: 22840694]
    HT-29 IC50
    278.08 6
    Compound: MTZ
    Cytotoxicity against human HT-29 cells after 24 hrs by MTT assay
    Cytotoxicity against human HT-29 cells after 24 hrs by MTT assay
    		[PMID: 30554970]
    HEK293 CC50
    > 100 3
    Compound: 1
    Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 24 hrs by resazurin assay
    Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 24 hrs by resazurin assay
    		[PMID: 27236016]
    KB IC50
    >100 3
    Compound: MNZ, metronidazole
    Cytotoxicity against human KB cells after 44 hrs by MTT assay
    Cytotoxicity against human KB cells after 44 hrs by MTT assay
    		[PMID: 18222017]
    HEK293 IC50
    > 100 3
    Compound: Metronidazole
    Cytotoxicity against HEK cells after 44 hrs by MTT assay
    Cytotoxicity against HEK cells after 44 hrs by MTT assay
    		[PMID: 18384916]
    KB IC50
    >100 3
    Compound: metronidazole (MNZ)
    Cytotoxicity against human KB cells after 44 hrs by MTT assay
    Cytotoxicity against human KB cells after 44 hrs by MTT assay
    		[PMID: 17513021]
    MCF7 IC50
    >250 3
    Compound: Metronidazole
    Cytotoxicity against Homo sapiens (human) MCF7 cells after 48 hr by MTT assay
    Cytotoxicity against Homo sapiens (human) MCF7 cells after 48 hr by MTT assay
    		10.1007/s00044-012-0107-x
    HT-29 IC50
    278.08 6
    Compound: MTZ
    Cytotoxicity against human HT-29 cells after 24 hrs by MTT assay
    Cytotoxicity against human HT-29 cells after 24 hrs by MTT assay
    		[PMID: 30554970]
    MCF7 IC50
    >250 3
    Compound: MNZ
    Cytotoxicity against Homo sapiens (human) MCF-7 cells assessed as decrease in number of viable cells after 48 hr by MTT assay
    Cytotoxicity against Homo sapiens (human) MCF-7 cells assessed as decrease in number of viable cells after 48 hr by MTT assay
    		10.1007/s00044-012-0070-6
    HeLa CC50
    285.7 3
    Compound: MTZ
    Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    		[PMID: 38461555]
    MCF7 IC50
    347.96 6
    Compound: MTZ
    Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay
    Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay
    		[PMID: 30554970]
    HeLa CC50
    666.2 3
    Compound: MTZ
    Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
    Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
    		[PMID: 38461555]
    MCF7 IC50
    >100 3
    Compound: Metronidazole
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    		[PMID: 20696501]
    HeLa EC50
    > 1000 6
    Compound: Metronidazole
    Cytotoxicity against human HeLa cells by MTT assay
    Cytotoxicity against human HeLa cells by MTT assay
    		[PMID: 22846917]
    HeLa IC50
    > 1000 6
    Compound: MTZ
    Cytotoxicity against human HeLa cells after 24 hrs by MTT assay
    Cytotoxicity against human HeLa cells after 24 hrs by MTT assay
    		[PMID: 24727243]
    MCF7 IC50
    >100 3
    Compound: Metronidazole
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    		[PMID: 20961670]
    MCF7 IC50
    >100 3
    Compound: metronidazole
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    		[PMID: 21377771]
    HeLa IC50
    > 1000 6
    Compound: MTZ
    Cytotoxicity against human HeLa cells
    Cytotoxicity against human HeLa cells
    		[PMID: 24900434]
    HeLa IC50
    > 5000 3
    Compound: MTZ
    Cytotoxicity against human HeLa cells after 24 hrs by MTT assay
    Cytotoxicity against human HeLa cells after 24 hrs by MTT assay
    		[PMID: 27643640]
    MCF7 IC50
    >250 3
    Compound: 1, MNZ
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    		[PMID: 22832309]
    HeLa IC50
    > 5000 3
    Compound: MTZ
    Cytotoxicity against human HeLa cells after 24 hrs by MTT assay
    Cytotoxicity against human HeLa cells after 24 hrs by MTT assay
    		[PMID: 29216562]
    MCF7 IC50
    >250 3
    Compound: MNZ
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    		[PMID: 22444681]
    MCF7 IC50
    >250 3
    Compound: Metronidazole
    Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by MTT assay
    		[PMID: 22309914]
    MDCK CC50
    68 3
    Compound: Met
    Cytotoxicity against MDCK cells after 72 hrs by MTT assay
    Cytotoxicity against MDCK cells after 72 hrs by MTT assay
    		[PMID: 19208443]
    Splenocyte IC50
    5.4 3
    Compound: Mtz
    Cytotoxicity against BALB/c mouse splenocytes assessed as [3H]-thymidine incorporation after 24 hrs by beta-radiation counting analysis relative to control
    Cytotoxicity against BALB/c mouse splenocytes assessed as [3H]-thymidine incorporation after 24 hrs by beta-radiation counting analysis relative to control
    		[PMID: 24530492]
    THP-1 IC50
    >500 3
    Compound: Metronidazole
    Cytotoxicity against human THP1 cells after 72 hrs by propidium iodide staining-based flow cytometry
    Cytotoxicity against human THP1 cells after 72 hrs by propidium iodide staining-based flow cytometry
    		[PMID: 19748781]
    THP-1 IC50
    >1460 3
    Compound: 5
    Cytotoxicity against human THP1 cells after 72 hrs by propidium iodide-based flow cytometry
    Cytotoxicity against human THP1 cells after 72 hrs by propidium iodide-based flow cytometry
    		[PMID: 18590939]
    Vero CC50
    >300 3
    Compound: MTZ
    Cytotoxicity against African green monkey Vero cells
    Cytotoxicity against African green monkey Vero cells
    		[PMID: 33556576]
    Vero CC50
    367 3
    Compound: Metronidazole
    Cytotoxicity against African green monkey Vero cells after 48 hrs by MTT assay
    Cytotoxicity against African green monkey Vero cells after 48 hrs by MTT assay
    		[PMID: 25801157]
    HepG2 CC50
    > 100 3
    Compound: 1
    Cytotoxicity against human HepG2 cells assessed as cell growth inhibition after 24 hrs by resazurin assay
    Cytotoxicity against human HepG2 cells assessed as cell growth inhibition after 24 hrs by resazurin assay
    		[PMID: 27236016]
    Vero CC50
    >16 6
    Compound: metronidazole
    Cytotoxicity against african green monkey Vero cells after 72 hrs by CellTiterGlo assay
    Cytotoxicity against african green monkey Vero cells after 72 hrs by CellTiterGlo assay
    		[PMID: 22691154]
    HepG2 IC50
    > 100 3
    Compound: MNZ
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    		[PMID: 22236470]
    Vero CC50
    387 3
    Compound: Metronidazole
    Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay
    Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay
    		[PMID: 21397502]
    Vero CC50
    387 3
    Compound: MNZ
    Cytotoxicity against African green monkey Vero cells assessed as cell viability after 48 hrs by SRB assay
    Cytotoxicity against African green monkey Vero cells assessed as cell viability after 48 hrs by SRB assay
    		[PMID: 24529307]
    HepG2 IC50
    > 100 3
    Compound: MNZ
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    		[PMID: 23644202]
    Vero CC50
    >600 3
    Compound: Metronidazole
    Cytotoxicity against african green monkey Vero cells assessed as inhibition of cell growth after 24 hrs by resazurin dye based fluorescence assay
    Cytotoxicity against african green monkey Vero cells assessed as inhibition of cell growth after 24 hrs by resazurin dye based fluorescence assay
    		[PMID: 27017556]
    HepG2 IC50
    > 100 3
    Compound: MNZ, Metronidazole
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    		[PMID: 22658085]
    HepG2 IC50
    > 100 3
    Compound: MNZ, Metronidazole
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    		[PMID: 22840694]
    Vero CC50
    >600 3
    Compound: MTZ
    Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell growth after 24 hrs by resazurin redox dye based fluorimetric microtiter method
    Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell growth after 24 hrs by resazurin redox dye based fluorimetric microtiter method
    		[PMID: 25771033]
    Vero CC50
    >300 3
    Compound: Metro
    Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 24 hrs by resazurin dye based fluorometric analysis
    Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 24 hrs by resazurin dye based fluorometric analysis
    		[PMID: 37729094]
    KB IC50
    > 100 3
    Compound: MNZ, metronidazole
    Cytotoxicity against human KB cells after 44 hrs by MTT assay
    Cytotoxicity against human KB cells after 44 hrs by MTT assay
    		[PMID: 18222017]
    Vero CC50
    387 3
    Compound: Metronidazole
    Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay
    Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay
    		[PMID: 28645659]
    KB IC50
    > 100 3
    Compound: metronidazole (MNZ)
    Cytotoxicity against human KB cells after 44 hrs by MTT assay
    Cytotoxicity against human KB cells after 44 hrs by MTT assay
    		[PMID: 17513021]
    Vero IC50
    >100 3
    Compound: MTZ
    Cytotoxicity against african green monkey Vero cells assessed as cell viability after 48 hrs by WST-1 assay
    Cytotoxicity against african green monkey Vero cells assessed as cell viability after 48 hrs by WST-1 assay
    		[PMID: 23787289]
    MCF7 IC50
    347.96 6
    Compound: MTZ
    Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay
    Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay
    		[PMID: 30554970]
    MCF7 IC50
    > 100 3
    Compound: Metronidazole
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    		[PMID: 20696501]
    MCF7 IC50
    > 100 3
    Compound: Metronidazole
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    		[PMID: 20961670]
    MCF7 IC50
    > 100 3
    Compound: metronidazole
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    		[PMID: 21377771]
    MCF7 IC50
    > 250 3
    Compound: 1, MNZ
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    		[PMID: 22832309]
    MCF7 IC50
    > 250 3
    Compound: MNZ
    Cytotoxicity against Homo sapiens (human) MCF-7 cells assessed as decrease in number of viable cells after 48 hr by MTT assay
    Cytotoxicity against Homo sapiens (human) MCF-7 cells assessed as decrease in number of viable cells after 48 hr by MTT assay
    		10.1007/s00044-012-0070-6
    MCF7 IC50
    > 250 3
    Compound: MNZ
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
    		[PMID: 22444681]
    MCF7 IC50
    > 250 3
    Compound: Metronidazole
    Cytotoxicity against Homo sapiens (human) MCF7 cells after 48 hr by MTT assay
    Cytotoxicity against Homo sapiens (human) MCF7 cells after 48 hr by MTT assay
    		10.1007/s00044-012-0107-x
    MCF7 IC50
    > 250 3
    Compound: Metronidazole
    Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by MTT assay
    		[PMID: 22309914]
    MDCK CC50
    68 3
    Compound: Met
    Cytotoxicity against MDCK cells after 72 hrs by MTT assay
    Cytotoxicity against MDCK cells after 72 hrs by MTT assay
    		[PMID: 19208443]
    Splenocyte IC50
    5.4 3
    Compound: Mtz
    Cytotoxicity against BALB/c mouse splenocytes assessed as [3H]-thymidine incorporation after 24 hrs by beta-radiation counting analysis relative to control
    Cytotoxicity against BALB/c mouse splenocytes assessed as [3H]-thymidine incorporation after 24 hrs by beta-radiation counting analysis relative to control
    		[PMID: 24530492]
    THP-1 IC50
    > 1460 3
    Compound: 5
    Cytotoxicity against human THP1 cells after 72 hrs by propidium iodide-based flow cytometry
    Cytotoxicity against human THP1 cells after 72 hrs by propidium iodide-based flow cytometry
    		[PMID: 18590939]
    THP-1 IC50
    > 500 3
    Compound: Metronidazole
    Cytotoxicity against human THP1 cells after 72 hrs by propidium iodide staining-based flow cytometry
    Cytotoxicity against human THP1 cells after 72 hrs by propidium iodide staining-based flow cytometry
    		[PMID: 19748781]
    Vero CC50
    367 3
    Compound: Metronidazole
    Cytotoxicity against African green monkey Vero cells after 48 hrs by MTT assay
    Cytotoxicity against African green monkey Vero cells after 48 hrs by MTT assay
    		[PMID: 25801157]
    Vero CC50
    387 3
    Compound: MNZ
    Cytotoxicity against African green monkey Vero cells assessed as cell viability after 48 hrs by SRB assay
    Cytotoxicity against African green monkey Vero cells assessed as cell viability after 48 hrs by SRB assay
    		[PMID: 24529307]
    Vero CC50
    387 3
    Compound: Metronidazole
    Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay
    Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay
    		[PMID: 21397502]
    Vero CC50
    387 3
    Compound: Metronidazole
    Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay
    Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay
    		[PMID: 28645659]
    Vero CC50
    > 16 6
    Compound: metronidazole
    Cytotoxicity against african green monkey Vero cells after 72 hrs by CellTiterGlo assay
    Cytotoxicity against african green monkey Vero cells after 72 hrs by CellTiterGlo assay
    		[PMID: 22691154]
    Vero CC50
    > 300 3
    Compound: MTZ
    Cytotoxicity against African green monkey Vero cells
    Cytotoxicity against African green monkey Vero cells
    		[PMID: 33556576]
    Vero CC50
    > 300 3
    Compound: Metro
    Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 24 hrs by resazurin dye based fluorometric analysis
    Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 24 hrs by resazurin dye based fluorometric analysis
    		[PMID: 37729094]
    Vero CC50
    > 600 3
    Compound: MTZ
    Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell growth after 24 hrs by resazurin redox dye based fluorimetric microtiter method
    Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell growth after 24 hrs by resazurin redox dye based fluorimetric microtiter method
    		[PMID: 25771033]
    Vero CC50
    > 600 3
    Compound: Metronidazole
    Cytotoxicity against african green monkey Vero cells assessed as inhibition of cell growth after 24 hrs by resazurin dye based fluorescence assay
    Cytotoxicity against african green monkey Vero cells assessed as inhibition of cell growth after 24 hrs by resazurin dye based fluorescence assay
    		[PMID: 27017556]
    Vero IC50
    > 100 3
    Compound: MTZ
    Cytotoxicity against african green monkey Vero cells assessed as cell viability after 48 hrs by WST-1 assay
    Cytotoxicity against african green monkey Vero cells assessed as cell viability after 48 hrs by WST-1 assay
    		[PMID: 23787289]
    In Vitro

    Metronidazole displays inhibitory activity towards anaerobic protozoa Trichomonas vaginalis, Entamoeba histolytica, Giardia lamblia, and Balantidium coli[1].
    Metronidazole (4-8 μg/mL) inhibits anaerobic bacteria and shows good bactericidal activity[1].
    Metronidazole (0.1 μg/mL-0.01 mg/mL; 12-96 h) induces granular formation and triggers apoptosis in Blastocystis sp[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Metronidazole Related Antibodies

    Apoptosis Analysis[2]

    Cell Line: Blastocystis sp. Cells
    Concentration: 0.1 μg/mL-0.01 mg/mL
    Incubation Time: 12, 24, 48, 60, 72, 84, 96 hours
    Result: Decreased cell diameter, as a hallmark of an apoptotic cell, and resulted cell shrinkage.
    In Vivo

    Metronidazole (135 mg/kg/d; p.o.; 28 d) can cross the blood brain barrier, and exhibits neurotoxicity under long term administration in rats[3].
    Metronidazole (1 g/L; p.o.; 4 weeks) results skeletal muscle atrophy and changes the expression of genes involved in the muscle peripheral circadian rhythm machinery and metabolic regulation[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Sprague-Dawley (SD) rats (200-220 g)[3]
    Dosage: 135 mg/kg
    Administration: Oral gavage; once daily; 28 days
    Result: Caused inflammatory markers increasing, including iNOS, eNOS, Bax and caspase 3 protein expressions increasing and caused oxidative stress damage in brain tissue, with MDA content rising.
    Animal Model: SPF C57Bl/6J mice (6-7 months old)[4]
    Dosage: 1 g/L
    Administration: Oral gavage; provided with drinking water for 4 weeks, changed twice weekly
    Result: Resulted the muscle core clock and effector genes Cry2, Ror-β, E4BP4, PP ARγ and adiponectin expression increasing.
    Decreased hind limb muscle weight and resulted in smaller fibers in the tibialis anterior muscle.
    Molecular Weight

    171.16

    Formula

    C6H9N3O3
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OCCN1C([N+]([O-])=O)=CN=C1C
    Structure Classification
    Initial Source

    Gram-negative and Gram-positive anaerobic
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 35 mg/mL (204.49 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 5 mg/mL (29.21 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.8425 mL 29.2124 mL 58.4249 mL
    5 mM 1.1685 mL 5.8425 mL 11.6850 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (12.15 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (12.15 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 12.5 mg/mL (73.03 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.96%

    SDS (419 KB)

    COA (253 KB) HNMR (239 KB) RP-HPLC (260 KB) MS (201 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 5.8425 mL 29.2124 mL 58.4249 mL 146.0622 mL
    5 mM 1.1685 mL 5.8425 mL 11.6850 mL 29.2124 mL
    10 mM 0.5842 mL 2.9212 mL 5.8425 mL 14.6062 mL
    15 mM 0.3895 mL 1.9475 mL 3.8950 mL 9.7375 mL
    20 mM 0.2921 mL 1.4606 mL 2.9212 mL 7.3031 mL
    25 mM 0.2337 mL 1.1685 mL 2.3370 mL 5.8425 mL
    DMSO 30 mM 0.1947 mL 0.9737 mL 1.9475 mL 4.8687 mL
    40 mM 0.1461 mL 0.7303 mL 1.4606 mL 3.6516 mL
    50 mM 0.1168 mL 0.5842 mL 1.1685 mL 2.9212 mL
    60 mM 0.0974 mL 0.4869 mL 0.9737 mL 2.4344 mL
    80 mM 0.0730 mL 0.3652 mL 0.7303 mL 1.8258 mL
    100 mM 0.0584 mL 0.2921 mL 0.5842 mL 1.4606 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Metronidazole Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Metronidazole443-48-1ParasiteEnvironmental PollutantsBacterialAntibioticApoptosisEnvironmental Contaminantsanaerobic infectionanaerobic protozoaTrichomonas vaginalisEntamoeba histolyticaGiardia lambliaBalantidium coli.BaxBcl2iNOSeNOScaspase-3Cry2Ror-βE4BP4. PP ARγadiponectinInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Metronidazole
    Cat. No.:
    HY-B0318
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (419 KB) Français - FR (419 KB) Deutsch - DE (419 KB) Norwegian - NO (419 KB) Español - ES (419 KB) Swedish - SV (419 KB) Italian - IT (419 KB) Korean - KR (419 KB) Portuguese - PT (419 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.