2. Anti-infection Apoptosis
  3. Antibiotic Bacterial Parasite Apoptosis
  4. Metronidazole hydrochloride

Metronidazole hydrochloride  (Synonyms: SC 326421)

Cat. No.: HY-B0318A Purity: 98.44%
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Metronidazole hydrochloride (SC 326421) is an orally active nitroimidazole antibiotic, can be used to research anaerobic infections. Metronidazole hydrochloride can cross blood brain barrier and results inflammation and skeletal muscle contraction under long-term application.

For research use only. We do not sell to patients.

CAS No. : 69198-10-3

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Oil + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Customer Review

Based on 65 publication(s) in Google Scholar

Other Forms of Metronidazole hydrochloride:

Metronidazole hydrochloride Related Antibodies

Top Publications Citing Use of Products

65 Publications Citing Use of MCE Metronidazole hydrochloride

WB
ELISA
In Vivo Imaging
PK/PD Analysis
Bio/Physico-chemical Assay
RT-PCR

    Metronidazole hydrochloride purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2025 Dec;17(1):2545434.  [Abstract]

    Western blot analysis of METTL3 and YTHDF2 protein expression from the CON and ABX (Ampicillin (1 g/L), Neomycin (1 g/L), Metronidazole (1 g/L), and vancomycin (0.5 g/L); drinking water from 6 to 12 weeks) mice (n = 3/group).

    Metronidazole hydrochloride purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2025 Dec;17(1):2545434.  [Abstract]

    RT-qPCR analysis of METTL3 and YTHDF2 protein expression from the CON and ABX (Ampicillin (1 g/L), Neomycin (1 g/L), Metronidazole (1 g/L), and vancomycin (0.5 g/L); drinking water from 6 to 12 weeks) mice (n = 3/group).

    Metronidazole hydrochloride purchased from MedChemExpress. Usage Cited in: J Exp Med. 2026 Mar 2;223(3):e20241287.  [Abstract]

    The number and frequency of MAIT cells in the ear pinnae and lungs of mice at 8 wk old following treatment with Metronidazole (30 mg/kg/day; oral gavage; from 2 to 4 weeks).

    Metronidazole hydrochloride purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2025 Nov 4;22(1):259.  [Abstract]

    Sufficiently enhanced susceptibility to JE by ampicillin (A) and/or Metronidazole (M) (1 g/L; drinking water; for 21 days) treatment in C57BL/6 mice.

    Metronidazole hydrochloride purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2023 Dec;15(2):2249143.  [Abstract]

    Targeted metabolomics analysis of gut microbial metabolites (AA, PA, and BA) in colon contents (feces) and serum from mice with or without ABX treatment (n = 6 per group). ABX: an antibiotic cocktail of 0.5 g/L Vancomycin, 1 g/L Metronidazole,1 g/L Neomycin and 1 g/L Ampicillin

    Metronidazole hydrochloride purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2023 Dec;15(2):2282790.  [Abstract]

    Depletion of CD8+ T cells in ABX-treated B16-F0 tumor-bearing mice dampened the antitumor effect of butyrate combined with anti-PD-1 (Figure 4b–e). Decreased serum IFN-γ and TNF-α production during the depletion of CD8+ T cells was also observed. ABX：an antibiotic cocktail of 0.5 g/L Vancomycin, 1 g/L Metronidazole, 1 g/L Neomycin and 1 g/L Ampicillin.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Metronidazole hydrochloride (SC 326421) is an orally active nitroimidazole antibiotic, can be used to research anaerobic infections. Metronidazole hydrochloride can cross blood brain barrier and results inflammation and skeletal muscle contraction under long-term application[1][2][3][4].

    In Vitro

    Metronidazole hydrochloride acts on anaerobic microorganism following: passage through the cell membrane; (b) reductive action; (c) interaction with intracellular targets; (d) release of inactive end products, exerts function with toxic nitro and nitroso derivatives[1].
    Metronidazole hydrochloride displays inhibitory activity towards anaerobic protozoa Trichomonas vaginalis, Entamoeba histolytica, Giardia lamblia, and Balantidium coli[1].
    Metronidazole hydrochloride (4-8 μg/mL) inhibits anaerobic bacteria and shows good bactericidal activity[1].
    Metronidazole hydrochloride (0.1 μg/mL-0.01 mg/mL; 12-96 h) induces granular formation and triggers apoptosis in Blastocystis sp[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Metronidazole hydrochloride Related Antibodies

    Apoptosis Analysis[2]

    Cell Line: Blastocystis sp. Cells
    Concentration: 0.1 μg/mL-0.01 mg/mL
    Incubation Time: 12, 24, 48, 60, 72, 84, 96 hours
    Result: Decreased cell diameter, as a hallmark of an apoptotic cell, and resulted cell shrinkage.
    In Vivo

    Metronidazole hydrochloride (135 mg/kg/d; p.o.; 28 d) can cross the blood brain barrier, and exhibits neurotoxicity under long term administration in rats[3].
    Metronidazole hydrochloride (1 g/L; p.o.; 4 weeks) results skeletal muscle atrophy and changes the expression of genes involved in the muscle peripheral circadian rhythm machinery and metabolic regulation[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Sprague-Dawley (SD) rats (200-220 g)[3]
    Dosage: 135 mg/kg
    Administration: Oral gavage; once daily; 28 days
    Result: Caused inflammatory markers increasing, including iNOS, eNOS, Bax and caspase 3 protein expressions increasing and caused oxidative stress damage in brain tissue, with MDA content rising.
    Animal Model: SPF C57Bl/6J mice (6-7 months old)[4]
    Dosage: 1 g/L (full dose)
    Administration: Oral gavage; provided with drinking water for 4 weeks, changed twice weekly
    Result: Resulted the muscle core clock and effector genes Cry2, Ror-β, E4BP4, PP ARγ and adiponectin expression increasing.
    Decreased hind limb muscle weight and resulted in smaller fibers in the tibialis anterior muscle.
    Clinical Trial
    Molecular Weight

    207.61

    Formula

    C6H10ClN3O3
    CAS No.
    Appearance

    Oil

    SMILES

    OCCN1C([N+]([O-])=O)=CN=C1C.[H]Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Pure form -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (481.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.8167 mL 24.0836 mL 48.1672 mL
    5 mM 0.9633 mL 4.8167 mL 9.6334 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (12.04 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (12.04 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.8167 mL 24.0836 mL 48.1672 mL 120.4181 mL
    5 mM 0.9633 mL 4.8167 mL 9.6334 mL 24.0836 mL
    10 mM 0.4817 mL 2.4084 mL 4.8167 mL 12.0418 mL
    15 mM 0.3211 mL 1.6056 mL 3.2111 mL 8.0279 mL
    20 mM 0.2408 mL 1.2042 mL 2.4084 mL 6.0209 mL
    25 mM 0.1927 mL 0.9633 mL 1.9267 mL 4.8167 mL
    30 mM 0.1606 mL 0.8028 mL 1.6056 mL 4.0139 mL
    40 mM 0.1204 mL 0.6021 mL 1.2042 mL 3.0105 mL
    50 mM 0.0963 mL 0.4817 mL 0.9633 mL 2.4084 mL
    60 mM 0.0803 mL 0.4014 mL 0.8028 mL 2.0070 mL
    80 mM 0.0602 mL 0.3010 mL 0.6021 mL 1.5052 mL
    100 mM 0.0482 mL 0.2408 mL 0.4817 mL 1.2042 mL
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    Metronidazole hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Metronidazole69198-10-3SC 326421SC326421SC-326421AntibioticBacterialParasiteApoptosisanaerobic infectionanaerobic protozoaTrichomonas vaginalisEntamoeba histolyticaGiardia lambliaBalantidium coli.BaxBcl2iNOSeNOScaspase-3Cry2Ror-βE4BP4. PP ARγadiponectinInhibitorinhibitorinhibit

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