2. GPCR/G Protein
    Neuronal Signaling
    Autophagy
    Immunology/Inflammation
    MAPK/ERK Pathway
    Apoptosis
  3. Dopamine Receptor
    Autophagy
    Mitophagy
    COX
    PGE synthase
    Interleukin Related
    p38 MAPK
    Apoptosis
    Caspase
  4. Oxidopamine hydrobromide

Oxidopamine hydrobromide  (Synonyms: 6-Hydroxydopamine hydrobromide; 6-OHDA hydrobromide)

Cat. No.: HY-B1081A Purity: 99.95%
COA Handling Instructions

Oxidopamine (6-OHDA) hydrobromide is an antagonist of the neurotransmitter dopamine. Oxidopamine hydrobromide is a widely used neurotoxin and selectively destroys dopaminergic neurons. Oxidopamine hydrobromide promotes COX-2 activation, leading to PGE2 synthesis and pro-inflammatory cytokine IL-1β secretion. Oxidopamine hydrobromide can be used for the research of Parkinson’s disease (PD), attention-deficit hyperactivity disorder (ADHD), and Lesch-Nyhan syndrome.

For research use only. We do not sell to patients.

Oxidopamine hydrobromide Chemical Structure

Oxidopamine hydrobromide Chemical Structure

CAS No. : 636-00-0

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of Oxidopamine hydrobromide:

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COX COX-1 COX-2 COX-3

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

  • Biological Activity

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Description

Oxidopamine (6-OHDA) hydrobromide is an antagonist of the neurotransmitter dopamine. Oxidopamine hydrobromide is a widely used neurotoxin and selectively destroys dopaminergic neurons. Oxidopamine hydrobromide promotes COX-2 activation, leading to PGE2 synthesis and pro-inflammatory cytokine IL-1β secretion. Oxidopamine hydrobromide can be used for the research of Parkinson’s disease (PD), attention-deficit hyperactivity disorder (ADHD), and Lesch-Nyhan syndrome[1][2][3][4].

IC50 & Target

COX-2

 

IL-1β

 

Caspase-3

 

Caspase-8

 

Caspase-9

 

In Vitro

Oxidopamine hydrobromide (0-500 µM, 24 h) decreases the viability of both Neuro-2a cells and SH-SY5Y cells in a concentration-dependent manner[1].
Oxidopamine hydrobromide (75-150 µM, 0-24 h) induces COX-2 expression and nuclear translocation[1].
Oxidopamine hydrobromide (75-150 µM, 0-24 h) causes PGE2 biosynthesis and pro-inflammatory cytokine IL-1β production[1].
Oxidopamine hydrobromide (0-150 μM, 12 h) induces apoptosis and mitochondrial membrane depolarization of pheochromocytoma PC12 cells[3].
Oxidopamine hydrobromide (75 μM, 0-12 h) induces p38 phosphorylation[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Neuro-2a cells and SH-SY5Y cells
Concentration: 0-500 µM
Incubation Time: 24 or 48 h
Result: Induced neurotoxicity, caused cytotoxicity in both Neuro-2a cells and SH-SY5Y cells in a concentration dependent manner. EC50=111 µM for 24 h incubation and 109 µM for 48 h incubation in the Neuro-2a cells; EC50=118 µM for 24 h incubation and 107 µM for 48 h incubation in the SH-SY5Y cells.

RT-PCR[1]

Cell Line: Neuro-2a cells and SH-SY5Y cells
Concentration: 75 or 150 µM
Incubation Time: 0, 6 or 24 h
Result: Quickly and robustly induced COX-2 in a time-dependent manner. Induced COX-2 activation characterized by expression induction and nuclear translocation. Substantially increased PGE2 in the culture medium by nearly 5-fold in Neuro-2a cells (at 75 µM) and 3-fold in SH-SY5Y cells (at 150 µM). Significantly upregulated the pro-inflammatory cytokine interleukin-1β (IL-1β) within Neuro-2a cells and SH-SY5Y cells.

Apoptosis Analysis[3]

Cell Line: PC12 cells
Concentration: 0, 25, 50, 75, and 150 μM
Incubation Time: 0, 2, 4, 6, 12, and 20 h
Result: Induced apoptosis of PC12 cells. Increased the activities of caspase-3, -8 and -9 in PC12 cells in a time- and concentration-dependent manner. Increased these caspase activities at 2-4 h and reached a maximum at 12 h. Decreased cells with high mitochondrial membrane potential (JC-1 aggregate) in a time- and concentration-dependent manner.

Western Blot Analysis[3]

Cell Line: PC12 cells
Concentration: 75 μM
Incubation Time: 0, 3, 5, 6, 8, 10, and 12 h
Result: Increased the level of p-p38 in a time-dependent manner.
In Vivo

Oxidopamine hydrobromide (5 µg/2 µL, unilaterally injected into the right striatum) induces degeneration of dopaminergic neurons in substantia nigra of rats[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

250.09

Appearance

Solid

Formula

C8H12BrNO3
CAS No.
SMILES

OC1=CC(CCN)=C(O)C=C1O.[H]Br
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (199.93 mM; ultrasonic and warming and heat to 60°C)

H2O : 20 mg/mL (79.97 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9986 mL 19.9928 mL 39.9856 mL
5 mM 0.7997 mL 3.9986 mL 7.9971 mL
10 mM 0.3999 mL 1.9993 mL 3.9986 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (199.93 mM); Clear solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (10.00 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (8.32 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.95%

COA (247 KB) HNMR (256 KB) LCMS (261 KB)

Handling Instructions (2659 KB)
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

Oxidopamine636-00-06-Hydroxydopamine6-OHDADopamine ReceptorAutophagyMitophagyCOXPGE synthaseInterleukin Relatedp38 MAPKApoptosisCaspaseMitochondrial AutophagyCyclooxygenaseProstaglandin E synthaseILneurotoxicityParkinson’s diseaseNeuro-2a cellsSH-SY5Y cellsattention-deficit hyperactivity disorderLesch-Nyhan syndromePC12 cellsp-p38PGE2Inhibitorinhibitorinhibit

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