2. Cell Cycle/DNA Damage Anti-infection Autophagy Apoptosis
  3. Topoisomerase Bacterial Fungal Autophagy Antibiotic DNA/RNA Synthesis c-Myc
  4. Idarubicin hydrochloride

Idarubicin hydrochloride  (Synonyms: 4-Demethoxydaunorubicin hydrochloride)

Cat. No.: HY-17381 Pureté: 99.94%
COA
SDS
Instruction de manipulation Technical Support

Idarubicin hydrochloride is an anthracycline antileukemic agent. It inhibits the topoisomerase II interfering with the replication of DNA and RNA transcription. Idarubicin hydrochloride inhibits the growth of bacteria and yeasts.

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CAS No. : 57852-57-0

Size Prix Stock Quantité
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
1 mg En stock
5 mg En stock
10 mg En stock
25 mg En stock
50 mg En stock
100 mg En stock
200 mg   Obtenir un devis  
500 mg   Obtenir un devis  

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Based on 17 publication(s) in Google Scholar

Other Forms of Idarubicin hydrochloride:

Idarubicin hydrochloride Related Antibodies

Top Publications Citing Use of Products

    Idarubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2024 Aug;26(8):1346-1358.  [Abstract]

    Number of total BM cells and GFP+ AF9 cells after treatment with 5 mg/kg DOX or 1 mg/kg Idarubicin hydrochloride for 5 days (n = 3 per group).

    Idarubicin hydrochloride purchased from MedChemExpress. Usage Cited in: iScience. 2024 Jun 8;27(7):110221.

    THP-1 and MOLM-13 cells (control for knockdown or shRHOF) were treated with 1.2 μM Ara-C and 0.03 μg/mL Idarubicin hydrochloride for 48 h to measure apoptosis via flow cytometry (n = 3). Student's t tests were used for comparing two variables.

    Idarubicin hydrochloride purchased from MedChemExpress. Usage Cited in: iScience. 2024 Jun 8;27(7):110221.

    THP-1 and MOLM-13 cells (control for overexpression or RHOF) were treated with 1.2 μM Ara-C and 0.03 μg/mL Idarubicin hydrochloride for 48 h to measure apoptosis via flow cytometry (n = 3). Student's t tests were used for comparing two variables.

    Idarubicin hydrochloride purchased from MedChemExpress. Usage Cited in: J Virol. 2019 May 15;93(11):e02230-18.  [Abstract]

    Idarubicin hydrochloride reduced HBV cccDNA levels in a dose-dependent manner, but had no effect on core DNA and DP-rcDNA levels.

    Idarubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2018 Apr 20;46(7):3284-3297.  [Abstract]

    NB4 and NB4-LR2 cells were treated with ZYH005, Arsenic trioxide (ATO), 5-fluorouracil (5-Fu), Cisplatin (DDP), Doxorubicin (DOX) and Idarubicin hydrochloride (IDA) at 0.05 μM for 24 h, and then cell apoptosis was determined.

    Voir tous les produits spécifiques à Isoform Topoisomerase:

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    Description

    Idarubicin hydrochloride is an anthracycline antileukemic agent. It inhibits the topoisomerase II interfering with the replication of DNA and RNA transcription. Idarubicin hydrochloride inhibits the growth of bacteria and yeasts.

    IC50 & Target[1]

    Topoisomerase II

     

    In Vitro

    The IC50 of Idarubicin hydrochloride is 3.3±0.4 ng/mL on MCF-7 monolayers and 7.9±1.1 ng/mL in multicellular spheroids[1]. Idarubicin hydrochloride has shown a greater cytotoxicity than daunorubicin or doxorubicin in various in vitro systems. This has been attributed to a better ability of idarubicin to induce the formation of topoisomerase II -mediated DNA breaks[2]. Idarubicin hydrochloride is about 57.5-fold and 25-fold more active than doxorubicin and epirubicin, respectively[3]. Idarubicin hydrochloride produces a concentration-dependent reduction in cell growth, with an IC50 value of approximately 0.01 μM. Idarubicin hydrochloride produced a concentration-dependent inhibition of DNA synthesis[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Idarubicin hydrochloride Related Antibodies
    Masse moléculaire

    533.95

    Formule

    C26H28ClNO9
    CAS No.
    Appearance

    Solid

    Color

    Orange to red

    SMILES

    OC(C(C(C1=CC=CC=C21)=O)=C3C2=O)=C4[C@H](C[C@@](C(C)=O)(O)CC4=C3O)O[C@@](O[C@@H](C)[C@H]5O)([H])C[C@@H]5N.Cl
    Livraison

    Room temperature in continental US; may vary elsewhere.
    Stockage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)

    Solvant et solubilité
    In Vitro: 

    DMSO : 83.33 mg/mL (156.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.8728 mL 9.3642 mL 18.7283 mL
    5 mM 0.3746 mL 1.8728 mL 3.7457 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Calculateur de molarité

    • Calculateur de dilution

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (3.90 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (3.90 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Pureté et documentation

    Purity: 99.94%

    SDS (643 KB)

    COA (256 KB) HNMR (303 KB) LCMS (92 KB)

    Instruction de manipulation (2659 KB)
    Références
    Test cellulaire
    [1]

    Stock solutions of idarubicin hydrochloride is dissolved in distilled water (1 mg/mL). MCF-7 monolayer are exposed to idarubicin or its metabolite idarubicinol at 0.01, 0.1, 1, 10, 100, and 1000 ng/mL for 24 hours. Multicellular spheroids are exposed to the same range of idarubicin　and idarubicinol concentration as monolayers (0.01-1000 ng/mL) for 24 h and, in separate experiments, at the drug concentration of 100 ng/mL for 6, 12, 24 and 48 h. The inhibition of cell proliferation is determined by counting the viable cells with an hemocytometer. Results are expressed as percentage of cell survival vs. control cultures[1].

    MCE n'a pas confirmé de manière indépendante l'exactitude de ces méthodes. Ils sont pour référence seulement.
    Références

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.8728 mL 9.3642 mL 18.7283 mL 46.8209 mL
    5 mM 0.3746 mL 1.8728 mL 3.7457 mL 9.3642 mL
    10 mM 0.1873 mL 0.9364 mL 1.8728 mL 4.6821 mL
    15 mM 0.1249 mL 0.6243 mL 1.2486 mL 3.1214 mL
    20 mM 0.0936 mL 0.4682 mL 0.9364 mL 2.3410 mL
    25 mM 0.0749 mL 0.3746 mL 0.7491 mL 1.8728 mL
    30 mM 0.0624 mL 0.3121 mL 0.6243 mL 1.5607 mL
    40 mM 0.0468 mL 0.2341 mL 0.4682 mL 1.1705 mL
    50 mM 0.0375 mL 0.1873 mL 0.3746 mL 0.9364 mL
    60 mM 0.0312 mL 0.1561 mL 0.3121 mL 0.7803 mL
    80 mM 0.0234 mL 0.1171 mL 0.2341 mL 0.5853 mL
    100 mM 0.0187 mL 0.0936 mL 0.1873 mL 0.4682 mL
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    Idarubicin hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Idarubicin57852-57-04-DemethoxydaunorubicinTopoisomeraseBacterialFungalAutophagyAntibioticDNA/RNA Synthesisc-MycMycleukaemiasMCF-7breast tumorDNA synthesisInhibitorinhibitorinhibit

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