Naphthazarin (Synonyms: DHNQ; 5,8-Dihydroxy-1,4-naphthoquinone)

Name: Naphthazarin
Brand: MedChemExpress
SKU: HY-N7526
Rating: 4.5 (1 reviews)

Cat. No.: HY-N7526 Purity: 99.59%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Naphthazarin (DHNQ) is a microtubule depolymerizing agent. Naphthazarin can improve motor function and reduce neuroinflammation in mouse models of Parkinson's disease. Naphthazarin can induce tumor cell apoptosis, autophagy, and cell cycle arrest. Naphthazarin can also induce erythrocyte apoptosis. Naphthazarin can be used in the research of tumors and neurodegenerative diseases.

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Naphthazarin 화학구조

CAS No. : 475-38-7

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
100 mg	해외재고보유	Estimated Time of Arrival: December 31
250 mg	해외재고보유	Estimated Time of Arrival: December 31
500 mg	견적 받기
1 g	견적 받기

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This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 publication(s) in Google Scholar

Other Forms of Naphthazarin:

Naphthazarin (Standard) In-stock

1 Publications Citing Use of MCE Naphthazarin

•Int J Biol Macromol. 2021 Jul 31:183:182-192.

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View All Isoforms

PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

View All Akt Isoform Specific Products:

View All Isoforms

Akt Akt1 Akt2 Akt3

Biological Activity
순도&문서
References
고객리뷰

제품 설명

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	8.85 μM Compound: SK-6	Antiproliferative activity against human A-375 cells assessed as inhibition of cell proliferation measured after 3 days by MTT assay Antiproliferative activity against human A-375 cells assessed as inhibition of cell proliferation measured after 3 days by MTT assay	[PMID: 39129245]
A2780	IC₅₀	0.08 μM Compound: 15	Antiproliferative activity against human A2780 cells after 2 days by alamar-blue assay Antiproliferative activity against human A2780 cells after 2 days by alamar-blue assay	[PMID: 19028102]
BT-549	GI₅₀	0.43 μM Compound: 1	Antiproliferative activity against human BT-549 cells measured after 72 hrs by SRB assay Antiproliferative activity against human BT-549 cells measured after 72 hrs by SRB assay	[PMID: 35687347]
CAL-51	GI₅₀	0.56 μM Compound: 1	Antiproliferative activity against human CAL-51 cells measured after 72 hrs by SRB assay Antiproliferative activity against human CAL-51 cells measured after 72 hrs by SRB assay	[PMID: 35687347]
HCC1954	GI₅₀	0.49 μM Compound: 1	Antiproliferative activity against human HCC1954 cells measured after 72 hrs by SRB assay Antiproliferative activity against human HCC1954 cells measured after 72 hrs by SRB assay	[PMID: 35687347]
HCT-116	GI₅₀	0.16 μM Compound: 1	Antiproliferative activity against human HCT-116 cells measured after 72 hrs by SRB assay Antiproliferative activity against human HCT-116 cells measured after 72 hrs by SRB assay	[PMID: 35687347]
HCT-116	IC₅₀	1.04 μM Compound: SK-6	Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell proliferation measured after 3 days by MTT assay Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell proliferation measured after 3 days by MTT assay	[PMID: 39129245]
HL-60	IC₅₀	0.8 μM Compound: 2	Cytotoxicity in human HL60 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity in human HL60 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 28987605]
HL-60	IC₅₀	0.93 μM Compound: 2	Cytotoxicity in human HL60 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity in human HL60 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 28987605]
HaCaT	IC₅₀	0.7 μM Compound: 7e ; Naphthazarin	Antiproliferative (inhibition of cell growth) activity against HaCaT cells (human keratinocyte line) Antiproliferative (inhibition of cell growth) activity against HaCaT cells (human keratinocyte line)	[PMID: 9371243]
HeLa	IC₅₀	9.37 μM Compound: 15	Inhibition of histidine-tagged mouse MKP1 catalytic domain expressed in human Hela cells Inhibition of histidine-tagged mouse MKP1 catalytic domain expressed in human Hela cells	[PMID: 19028102]
HepG2	IC₅₀	4.97 μM Compound: SK-6	Antiproliferative activity against human HepG2 cells assessed as inhibition of cell proliferation measured after 3 days by MTT assay Antiproliferative activity against human HepG2 cells assessed as inhibition of cell proliferation measured after 3 days by MTT assay	[PMID: 39129245]
K562	GI₅₀	1.7 μM Compound: 1	Antiproliferative activity against human K562 cells measured after 72 hrs by SRB assay Antiproliferative activity against human K562 cells measured after 72 hrs by SRB assay	[PMID: 35687347]
MCF7	GI₅₀	0.43 μM Compound: 1	Antiproliferative activity against human MCF7 cells measured after 72 hrs by SRB assay Antiproliferative activity against human MCF7 cells measured after 72 hrs by SRB assay	[PMID: 35687347]
MOLM-13	GI₅₀	0.28 μM Compound: 1	Antiproliferative activity against human MOLM-13 cells measured after 72 hrs by SRB assay Antiproliferative activity against human MOLM-13 cells measured after 72 hrs by SRB assay	[PMID: 35687347]
MV4-11	GI₅₀	0.19 μM Compound: 1	Antiproliferative activity against human MV4-11 cells measured after 72 hrs by SRB assay Antiproliferative activity against human MV4-11 cells measured after 72 hrs by SRB assay	[PMID: 35687347]

In Vitro

Naphthazarin (0-10 μM; 24 h) can stimulate apoptosis in human red blood cells by increasing oxidative stress and surface ceramide abundance^[1].
Naphthazarin (0-80 μM; 24 h) can inhibit the cell viability, induce cell apoptosis, autophagy and cell cycle arrest, and suppress the PI3K/Akt pathway in A549 cells^[2].
Naphthazarin (10-1000 nM; 30 h) can alleviate MPP⁺-induced activation in primary cultured rat astrocytes^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:	Primary cultured astrocyte treated MPP⁺
Concentration:	10 nM, 100 nM and 1 μM
Incubation Time:	Pretreated with 6 h, then co-incubation for 24 h
Result:	Inhibited the levels of GFAP.

In Vivo

Naphthazarin (0.1-1 mg/kg; intraperitoneal injection; 5 days) exerts a protective effect in a mouse model of Parkinson's disease^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/C mice aged 7-8 weeks old weighing approximately 20-23 g) treated MPTP (HY-W114750) to induce Parkinson's disease model^[3]
Dosage:	0.1 mg/kg and 1 mg/kg
Administration:	Intraperitoneal injection; 5 days
Result:	Improved the motor function of the mice at 1 mg/kg Protected dopaminergic neurons and inhibited neuroinflammation in the mice at 0.1 mg/kg and 1 mg/kg. Suppressed MPTP-induced glial activation at 0.1 mg/kg and 1 mg/kg.

분자량

190.15

화학식

C₁₀H₆O₄

CAS No.

475-38-7

Appearance

Solid

Color

Brown to black

SMILES

O=C1C=CC(C2=C1C(O)=CC=C2O)=O

Structure Classification

Initial Source

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : 10 mg/mL (52.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.2590 mL	26.2950 mL	52.5901 mL
5 mM	1.0518 mL	5.2590 mL	10.5180 mL
10 mM	0.5259 mL	2.6295 mL	5.2590 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 0.71 mg/mL (3.73 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 0.71 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (7.1 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 99.59%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (255 KB)

Handling Instructions (2659 KB)

References

[1]. Omar Aljanadi, et al. Stimulation of Suicidal Erythrocyte Death by Naphthazarin. Basic Clin Pharmacol Toxicol. 2015 Dec;117(6):369-74. [Content Brief]

[2]. Acharya BR, et al. The microtubule depolymerizing agent naphthazarin induces both apoptosis and autophagy in A549 lung cancer cells. Apoptosis. 2011 Sep;16(9):924-39. doi: 10.1007/s10495-011-0613-1 [Content Brief]

[3]. Seon Young Choi, et al. Naphthazarin has a protective effect on the 1-methyl-4-phenyl-1,2,3,4-tetrahydropyridine-induced Parkinson's disease model. J Neurosci Res. 2012 Sep;90(9):1842-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.2590 mL	26.2950 mL	52.5901 mL	131.4752 mL
	5 mM	1.0518 mL	5.2590 mL	10.5180 mL	26.2950 mL
	10 mM	0.5259 mL	2.6295 mL	5.2590 mL	13.1475 mL
	15 mM	0.3506 mL	1.7530 mL	3.5060 mL	8.7650 mL
	20 mM	0.2630 mL	1.3148 mL	2.6295 mL	6.5738 mL
	25 mM	0.2104 mL	1.0518 mL	2.1036 mL	5.2590 mL
	30 mM	0.1753 mL	0.8765 mL	1.7530 mL	4.3825 mL
	40 mM	0.1315 mL	0.6574 mL	1.3148 mL	3.2869 mL
	50 mM	0.1052 mL	0.5259 mL	1.0518 mL	2.6295 mL

Naphthazarin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Naphthazarin475-38-7DHNQ 5,8-Dihydroxy-1,4-naphthoquinoneApoptosisAutophagyPI3KAktPhosphoinositide 3-kinasePKBProtein kinase Bhuman red blood cellsA549 cellsastrocytesPD modelInhibitorinhibitorinhibit

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Naphthazarin (Synonyms: DHNQ; 5,8-Dihydroxy-1,4-naphthoquinone)

고객리뷰

Other Forms of Naphthazarin:

Naphthazarin Related Antibodies

1 Publications Citing Use of MCE Naphthazarin

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All PI3K Isoform Specific Products:

View All Akt Isoform Specific Products:

Biological Activity

순도&문서

References

고객리뷰

Naphthazarin Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

Naphthazarin Related Classifications

Keywords:

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고객리뷰

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SAFETY DATA SHEET (SDS)

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