2. Anti-infection Cell Cycle/DNA Damage
  3. Bacterial Antibiotic Topoisomerase DNA/RNA Synthesis Orthopoxvirus
  4. Levofloxacin hydrate

Levofloxacin hydrate  (Synonyms: Levofloxacin hemihydrate)

Cat. No.: HY-B0330A Purity: 99.98%
COA
SDS
Handling Instructions Technical Support

Levofloxacin hydrate (Levofloxacin hemihydrate) is an orally active antibiotic and is active against both Gram-positive and Gram-negative bacteria. Levofloxacin hydrate inhibits the DNA gyrase and topoisomerase IV. Levofloxacin hydrate can be used for chronic periodontitis, airway inflammation and BK Viremia research. Levofloxacin hydrate shows anti-orthopoxvirus activity.

For research use only. We do not sell to patients.

CAS No. : 138199-71-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
100 mg In-stock
5 g In-stock
10 g   Get quote  
50 g   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 35 publication(s) in Google Scholar

Other Forms of Levofloxacin hydrate:

Levofloxacin hydrate Related Antibodies

Top Publications Citing Use of Products

35 Publications Citing Use of MCE Levofloxacin hydrate

Microbiological Assay
Cell Proliferation/Viability Assay

    Levofloxacin hydrate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 May 10;16(1):4366.  [Abstract]

    M. bovis BCG Russia WT in LFX (0-25 h) 1 mg/L.

    Levofloxacin hydrate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 May 10;16(1):4366.  [Abstract]

    M. bovisBCG Russia FQR in LFX (0-25 h) 1 mg/L.

    Levofloxacin hydrate purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Dec;15(12):6382-6398.

    Levofloxacin exhibited toxicity at 25 × Cmax, which resulted in a 65% reduction in organoid cell viability.

    Levofloxacin hydrate purchased from MedChemExpress. Usage Cited in: EBioMedicine. 2025 May 30:117:105776.  [Abstract]

    Killing curves of different Ms rpoB mutants after exposure to LFX (0-48 h) at 1 μg/mL (5 × MIC) in 7H9 medium.

    Levofloxacin hydrate purchased from MedChemExpress. Usage Cited in: Antibiotics (Basel). 2022 Feb 1;11(2):192.

    Determined minimum inhibitory concentrations (MICs) of Gepotidacin, Levofloxacin, Moxifloxacin, and Co-trimoxazole against S. maltophilia.

    View All Antibiotic Isoform Specific Products:

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    View All Topoisomerase Isoform Specific Products:

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    Topoisomerase Topo I Topo II DNA gyrase

    View All DNA/RNA Synthesis Isoform Specific Products:

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    RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Levofloxacin hydrate (Levofloxacin hemihydrate) is an orally active antibiotic and is active against both Gram-positive and Gram-negative bacteria. Levofloxacin hydrate inhibits the DNA gyrase and topoisomerase IV. Levofloxacin hydrate can be used for chronic periodontitis, airway inflammation and BK Viremia research. Levofloxacin hydrate shows anti-orthopoxvirus activity[1][2][3][4].

    IC50 & Target

    TOPO IV

     

    In Vitro

    Levofloxacin shows inhibition effects to M. tuberculosis susceptible strains OFLO, LVFX, and SPFX with MIC50 values of 1.0, 0.5 and 0.25 μg/mL, respectively[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Levofloxacin hydrate Related Antibodies
    In Vivo

    Levofloxacin (10.7 mg/kg; i.p., once daily for 10 days or 3 weeks) time-dependently induces toxic effects on liver and heart in albino mice[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Matured male Albino mice[4]
    Dosage: 10.7 mg/kg
    Administration: Intraperitoneal injection; 10.7 mg/kg, once daily for 10 days or 3 weeks
    Result: Induced severe congestion of blood vessels in the portal area, central veins with inflammatory cells infiltration, necrosis with pyknosis of cardiac muscle nuclei and apoptosis, degeneration and necrosis of hepatocytes.
    Molecular Weight

    370.38

    Formula

    C18H21FN3O4.5
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(C(C1=O)=CN2[C@@H](C)COC3=C(N4CCN(C)CC4)C(F)=CC1=C23)O.[0.5H2O]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (135.00 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 10 mg/mL (27.00 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6999 mL 13.4996 mL 26.9993 mL
    5 mM 0.5400 mL 2.6999 mL 5.3999 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 5 mg/mL (13.50 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 5 mg/mL (13.50 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.98%

    SDS (419 KB)

    COA (252 KB) HNMR (283 KB) LCMS (93 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.6999 mL 13.4996 mL 26.9993 mL 67.4982 mL
    5 mM 0.5400 mL 2.6999 mL 5.3999 mL 13.4996 mL
    10 mM 0.2700 mL 1.3500 mL 2.6999 mL 6.7498 mL
    15 mM 0.1800 mL 0.9000 mL 1.8000 mL 4.4999 mL
    20 mM 0.1350 mL 0.6750 mL 1.3500 mL 3.3749 mL
    25 mM 0.1080 mL 0.5400 mL 1.0800 mL 2.6999 mL
    DMSO 30 mM 0.0900 mL 0.4500 mL 0.9000 mL 2.2499 mL
    40 mM 0.0675 mL 0.3375 mL 0.6750 mL 1.6875 mL
    50 mM 0.0540 mL 0.2700 mL 0.5400 mL 1.3500 mL
    60 mM 0.0450 mL 0.2250 mL 0.4500 mL 1.1250 mL
    80 mM 0.0337 mL 0.1687 mL 0.3375 mL 0.8437 mL
    100 mM 0.0270 mL 0.1350 mL 0.2700 mL 0.6750 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Levofloxacin hydrate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Levofloxacin138199-71-0Levofloxacin hemihydrateBacterialAntibioticTopoisomeraseDNA/RNA SynthesisOrthopoxviruschronic periodontitisairway inflammationBK ViremiaDNA gyraseInhibitorinhibitorinhibit

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