Art. -Nr.: HY-B0330

Levofloxacin

Name: Levofloxacin
Brand: MedChemExpress
SKU: HY-B0330
Price: 66.00 USD
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41 Cited Publications

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Based on 41 publication(s) in Google Scholar

Levofloxacin is an orally active broad-spectrum antibacterial agent with antioxidant and antibacterial activities. Levofloxacin upregulates TC synthesis-related genes and transport-related genes, reduces plasma PCSK9, inhibits GABA_A receptors and mitochondrial complexes I/III, scavenges hydroxyl radicals and neutrophil-derived ROS, and inhibits NO metabolites and interferon-γ. Levofloxacin improves survival, alleviates lung injury, and reduces oxidative and inflammatory markers in influenza virus-infected mice. Levofloxacin inhibits lung cancer proliferation and xenograft tumor growth, and induces apoptosis and oxidative stress. Levofloxacin can be used in research on bacterial infection, influenza virus infection, atherosclerosis, and lung cancer.

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Reinheit : 99.92%
CAS. Nr.: 100986-85-4
Formel: C₁₈H₂₀FN₃O₄
Molecular Weight:361.37

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Levofloxacin Chemical Structure

CAS. Nr.: 100986-85-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (41)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle Topoisomerase Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
Vero	CC₅₀	>100 μg/mL Compound: LEVO	Cytotoxicity against african green monkey Vero cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 37731699]

In Vitro

Anti-cancerApoptosisBacterial InfectionInnate ImmunityLipid MetabolismMitochondrial FunctionMitochondrial Oxidative PhosphorylationOxidative Stress

Anti-cancer

Levofloxacin (50-200 mg/mL; 72 h) inhibits the proliferation of A549, H3255, NCL-H69, and H460 lung cancer cells^[4].

Anti-cancer

Apoptosis

Levofloxacin (50-200 mg/mL; 72 h) induces Annexin V-positive apoptosis in A549, H3255, NCL-H69, and H460 cells^[4].

Bacterial Infection

Levofloxacin shows MIC50 values of 1.0, 0.5, and 0.25 μg/mL against the susceptible Mycobacterium tuberculosis strains OFLO, LVFX, and SPFX, respectively^[4].

Innate Immunity

Lipid Metabolism

Levofloxacin (LEV) (0.05-0.2 mg/mL; 24 h) reduces ox-LDL-induced foam cell formation in Raw264.7 macrophages and decreases oleic acid-induced lipid deposition in HepG-2 cells^[2].

Mitochondrial Function

Mitochondrial Oxidative Phosphorylation

Levofloxacin (50-200 µg/mL; 24 h) reduces basal and maximal mitochondrial respiration and decreases ATP levels in A549 and H460 cells; it inhibits mitochondrial ETC complexes I and III, but not complexes II and IV; it increases intracellular ROS, mitochondrial superoxide, and extracellular hydrogen peroxide^[4].

Oxidative Stress

Levofloxacin (20-200 μM; 30 s) scavenges hydroxyl radicals generated by H₂O₂/UV in cell-free DMPO ESR experiments, and 200 μM is sufficient to inhibit the DMPO-OH signal^[1].

Oxidative Stress

Innate Immunity

Levofloxacin (50-1000 μM; 7 min) scavenges PMA-stimulated mouse neutrophil-derived ROS in the DMPO ESR assay, requiring 1000 μM to achieve a significant reduction^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Anti-cancerApoptosisOxidative StressLipid MetabolismViral InfectionCytokines & ChemokinesOthers

Anti-cancer

Apoptosis

Oxidative Stress

Levofloxacin (100 mg/kg; intraperitoneal injection) inhibits H460 lung cancer growth in SCID mice, reduces tumor volume and weight, and simultaneously decreases proliferation and increases apoptosis and oxidative stress markers^[4].

Lipid Metabolism

Levofloxacin reduces aortic lipid deposition and plasma lipids in high-fat diet ApoE^-/- mice and upregulates hepatic lipoprotein receptors and cholesterol transport genes, with minimal effect on DGAT1/MTTP-related TG synthesis and VLDL secretion^[2].

Viral Infection

Oxidative Stress

Cytokines & Chemokines

Levofloxacin (25-100 mg/kg; intraperitoneal injection; once daily; days 2-6) improves the survival rate of PR8 influenza-infected mice, alleviates lung histological injury, oxidative/nitrative stress, and NOx/IFN-γ levels in BALF, without reducing pulmonary viral titers^[1].

Others

Levofloxacin (10-40 mg/kg; intraperitoneal injection; single administration) produces anxiety-like effects in the rat elevated plus maze test; shortens sedative-hypnotic-induced sleep duration without affecting sleep latency; reduces acetic acid-induced writhing responses and prolongs hot plate latency^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Chemical Information

CAS. Nr. 100986-85-4
Appearance Solid
Molecular Weight 361.37
Formel C₁₈H₂₀FN₃O₄
Color Off-white to light yellow
SMILES

O=C(C(C1=O)=CN2[C@@H](C)COC3=C(N4CCN(C)CC4)C(F)=CC1=C23)O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (41)

All Microbiological AssayCell Proliferation/Viability Assay

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Journal Impact Factor

Signal Transduct Target Ther

Selective depletion of tumor-associated SAMHD1 enhances chemotherapeutic efficacy and antitumor immune responses. [Abstract]

2025 Dec 15;10(1):406. PMID: 41392286
Nat Commun

Phenotypic drug susceptibility testing for Mycobacterium tuberculosis variant bovis BCG in 12 hours. [Abstract]

2025 May 10;16(1):4366. PMID: 40348759

Levofloxacin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 May 10;16(1):4366. [Abstract]

M. bovis BCG Russia WT in LFX (0-25 h) 1 mg/L.

Levofloxacin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 May 10;16(1):4366. [Abstract]

M. bovisBCG Russia FQ^R in LFX (0-25 h) 1 mg/L.
Nat Commun

Antimicrobial resistance and population genomics of multidrug-resistant Escherichia coli in pig farms in mainland China. [Abstract]

2022 Mar 2;13(1):1116. PMID: 35236849
Acta Pharm Sin B

Generation and applications of an expandable and mature hiPSC-derived liver organoid. [Abstract]

2025 Dec;15(12):6382-6398. PMID: 41477334

Levofloxacin purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Dec;15(12):6382-6398. [Abstract]

Levofloxacin exhibited toxicity at 25 × C_max, which resulted in a 65% reduction in organoid cell viability.
Sci Adv

Quantitative prediction of siRNA complexation by ionizable drugs enables their codelivery in nanoparticles. [Abstract]

2026 Jun 12;12(24):eaed2731. PMID: 42284421
Chem Eng J

2026 Feb 12.
J Exp Med

Antibiotic use in early life impairs MAIT cell-mediated immunity in adulthood. [Abstract]

2026 Mar 2;223(3):e20241287. PMID: 41400657
EBioMedicine

Diverse impacts of different rpoB mutations on the anti-tuberculosis efficacy of capreomycin. [Abstract]

2025 Jul:117:105776. PMID: 40449326

Levofloxacin purchased from MedChemExpress. Usage Cited in: EBioMedicine. 2025 Jul:117:105776. [Abstract]

Killing curves of different Ms rpoB mutants after exposure to LFX (0-48 h) at 1 μg/mL (5 × MIC) in 7H9 medium.
Food Res Int

Prevalence, plasmid transmission, and chromosomal integration of blaCTX-M genes in Salmonella isolated from retail chicken and pork meats in China. [Abstract]

2026 Aug 31:238:119421. PMID: 42215089
Clin Chem

Discovering Cross-Reactivity in Urine Drug Screening Immunoassays through Large-Scale Analysis of Electronic Health Records. [Abstract]

2019 Dec;65(12):1522-1531. PMID: 31578215
Emerg Microbes Infect

AMXT-1501 targets membrane phospholipids against Gram-positive and -negative multidrug-resistant bacteria. [Abstract]

2024 Dec;13(1):2321981. PMID: 38422452
Virulence

Antibacterial efficacy and mechanism of the novel antimicrobial peptide lachnospirin-1 against Acinetobacter baumannii. [Abstract]

2026 Dec 31;17(1):2646808. PMID: 41838520
Commun Biol

The acetyltransferase CysE modulates virulence and drug resistance of Mycobacterium tuberculosis by interfering with oxidative stress responses. [Abstract]

2025 Oct 6;8(1):1425. PMID: 41053365
Results Chem

2024 Oct.
Cell Rep Methods

RECOVER identifies synergistic drug combinations in vitro through sequential model optimization. [Abstract]

2023 Oct 23;3(10):100599. PMID: 37797618
Antibiotics (Basel)

Antibacterial Activity of the Novel Drug Gepotidacin against Stenotrophomonas maltophilia-An In Vitro and In Vivo Study. [Abstract]

2022 Feb 1;11(2):192. PMID: 35203795

Levofloxacin purchased from MedChemExpress. Usage Cited in: Antibiotics (Basel). 2022 Feb 1;11(2):192. [Abstract]

Determined minimum inhibitory concentrations (MICs) of Gepotidacin, Levofloxacin, Moxifloxacin, and Co-trimoxazole against S. maltophilia.
Int J Antimicrob Agents

Elucidating adaptive compensatory tigecycline resistance mechanisms of RamA, RarA and SoxS in Klebsiella pneumoniae. [Abstract]

2025 Oct;66(4):107551. PMID: 40499596
PLoS Pathog

Anti-orthopoxvirus drugs inhibit lumpy skin disease virus replication by targeting viral DNA polymerase. [Abstract]

2026 Jan 26;22(1):e1013903. PMID: 41587213
Toxics

The Acute Toxicity and Cardiotoxic Effects of Levofloxacin on Zebrafish (Danio rerio). [Abstract]

2025 Feb 5;13(2):122. PMID: 39997937
iScience

2026 Aug 28;29(9):117291. PMID: 42701369
ACS Infect Dis

Lusutrombopag as a Repurposing Drug in Combination with Aminoglycosides against Vancomycin-Resistant Enterococcus. [Abstract]

2024 Apr 12;10(4):1327-1338. PMID: 38567846
Antimicrob Agents Chemother

Dihydrotanshinone I Is Effective against Drug-Resistant Helicobacter pylori In Vitro and In Vivo. [Abstract]

2021 Feb 17;65(3):e01921-20. PMID: 33318002
Microbiol Spectr

Montelukast treats Streptococcus pneumoniae-induced sepsis via antibacterial and anti-inflammatory activities. [Abstract]

2025 Oct 15:e0122125. PMID: 41090944
Microbiol Spectr

Antimicrobial resistance of rapidly growing mycobacteria isolated from companion animals in Taiwan. [Abstract]

2025 Jul;13(7):e0307424. PMID: 40387379
AMB Express

Repurposing pinaverium bromide against Staphylococcus and its biofilms with new mechanisms. [Abstract]

2024 Dec 24;14(1):141. PMID: 39718732
J Med Virol

Drug Repurposing: In Vitro Evaluation of Simeprevir as a Novel Antiviral Drug Against Severe Fever With Thrombocytopenia Syndrome Virus. [Abstract]

2025 Oct;97(10):e70655. PMID: 41117261
Water

2025 Jun 6.
Infect Drug Resist

2026 May 8;19.
Eur J Clin Microbiol Infect Dis

Evaluation of EUCAST rapid antimicrobial susceptibility testing (RAST) for carbapenemase-producing Klebsiella pneumoniae with focus on KPC variants. [Abstract]

2025 Sep 29. PMID: 41023358
PLoS One

2026 Jul 8;21(7):e0352997. PMID: 42418411
Infect Genet Evol

Polymyxin B combined with amikacin delays the resistance of Klebsiella pneumoniae to polymyxin B by modulating the expression of NlpE. [Abstract]

2025 Sep:133:105780. PMID: 40480594
PLoS One

In vitro activity of ceftazidime/avibactam, cefiderocol, meropenem/vaborbactam and imipenem/relebactam against clinical strains of the Stenotrophomonas maltophilia complex. [Abstract]

2024 Apr 18;19(4):e0298577. PMID: 38635685
Future Microbiol

Synergistic drug strategy against mastitis-associated MRSA strains. [Abstract]

2025 Oct 13:1-10. PMID: 41082411
Chin J Traumatol

The cytochrome P4501A1 (CYP1A1) inhibitor bergamottin enhances host tolerance to multidrug-resistant Vibrio vulnificus infection. [Abstract]

2024 Sep;27(5):295-304. PMID: 38981821
Diagn Microbiol Infect Dis

2026 Nov;116(3):117584. PMID: 42531941
Diagn Microbiol Infect Dis

A rapid bacterial labeling method for phenotypic antimicrobial susceptibility testing. [Abstract]

2026 Feb;114(2):117181. PMID: 41205476
Res Sq

2026 Mar 9.
SSRN

2025 Oct 14.
Res Sq

2025 Aug 05.
bioRxiv

2025 January 19.
bioRxiv

2024 May 10.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 10 mg/mL (27.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 5 mg/mL (13.84 mM)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.7672 mL

13.8362 mL

27.6725 mL

5 mM

0.5534 mL

2.7672 mL

5.5345 mL

10 mM

0.2767 mL

1.3836 mL

2.7672 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1 mg/mL (2.77 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 1 mg/mL (2.77 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1 mg/mL (2.77 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 10 mg/mL (27.67 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (291 KB)
SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Enoki Y, et al. Pleiotropic Effects of Levofloxacin, Fluoroquinolone Antibiotics, against Influenza Virus-Induced Lung Injury. PloS one. 2015;10(6):e0130248.

[2]. Sheng Q H, et al. Levofloxacin Ameliorates Atherosclerosis By Regulating Lipid Metabolism. 2025.

[3]. Erden BF, et al. Antidepressant, anxiogenic, and antinociceptive properties of levofloxacin in rats and mice. Pharmacology, biochemistry, and behavior. 2001 Mar;68(3):435-41. [Content Brief]

[4]. Song M, et al. Antibiotic drug levofloxacin inhibits proliferation and induces apoptosis of lung cancer cells through inducing mitochondrial dysfunction and oxidative damage. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 2016 Dec;84:1137-1143. [Content Brief]

[5]. JI B, et al. In vitro and in vivo activities of levofloxacin against Mycobacterium tuberculosis. Antimicrob Agents Chemother. 1995 Jun;39(6):1341-4. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	2.7672 mL	13.8362 mL	27.6725 mL	69.1812 mL
	5 mM	0.5534 mL	2.7672 mL	5.5345 mL	13.8362 mL
	10 mM	0.2767 mL	1.3836 mL	2.7672 mL	6.9181 mL
DMSO	15 mM	0.1845 mL	0.9224 mL	1.8448 mL	4.6121 mL
	20 mM	0.1384 mL	0.6918 mL	1.3836 mL	3.4591 mL
	25 mM	0.1107 mL	0.5534 mL	1.1069 mL	2.7672 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Bioz

Levofloxacin

41 Cited Publications

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Levofloxacin

Publications Citing Use of MedChemExpress (MCE) Levofloxacin

Alle Topoisomerase Isoform-spezifische Produkte anzeigen