(-)-Menthol

Name: (-)-Menthol
Brand: MedChemExpress
SKU: HY-75161
Rating: 5.0 (4 reviews)

Cat. No.: HY-75161 Pureté: 98.0%: Fiche technique
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(-)-Menthol is a key component of peppermint oil that binds and activates transient receptor potential melastatin 8 (TRPM8), a Ca²⁺-permeable nonselective cation channel, to increase [Ca²⁺]_i. Antitumor activity.

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CAS No. : 2216-51-5

Size	Stock	Quantité
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid or liquid
500 mg	En stock	Estimated Time of Arrival: December 31
1 g	En stock	Estimated Time of Arrival: December 31
5 g	En stock	Estimated Time of Arrival: December 31
10 g	En stock	Estimated Time of Arrival: December 31
25 g	En stock	Estimated Time of Arrival: December 31
50 g	En stock	Estimated Time of Arrival: December 31
100 g	En stock	Estimated Time of Arrival: December 31
> 100 g	Obtenir un devis

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Based on 4 publication(s) in Google Scholar

Other Forms of (-)-Menthol:

(-)-Menthol (Standard) In-stock
(-)-Menthol-d₄ Obtenir un devis

4 Publications Citing Use of MCE (-)-Menthol

Histological Imaging/Staining

: (-)-Menthol purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2023 Jan 3;64(1):19. [Abstract]; Topical administration of the TRPM8 agonist (-)-menthol (200 µM; four times per day for 2 d) to eyes of C57BL/6J mice after debridement delayed corneal epithelial healing.

Recommandations en vedette

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Voir tous les produits spécifiques à Isoform TRP Channel:

Voir toutes les isoformes

TRPA1 TRPC3 TRPC4 TRPC5 TRPC6 TRPC7 TRPM8 TRPM2 TRPM3 TRPM4 TRPM7 TRPML1 TRPML2 TRPML3 TRPV1 TRPV2 TRPV3 TRPV4 TRPV6

Voir tous les produits spécifiques à Isoform Endogenous Metabolite:

Voir toutes les isoformes

Human Endogenous Metabolite Microbial Metabolite

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Description

IC₅₀ & Target

TRPM8^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	10.1 3 Compound: L-Menthol	Agonist activity at full length recombinant human TRPM8 expressed in HEK293 cells assessed as increase in intracellular Ca2+ level pre-incubated for 5 mins by Fluo4-AM dye-based fluorescence assay Agonist activity at full length recombinant human TRPM8 expressed in HEK293 cells assessed as increase in intracellular Ca2+ level pre-incubated for 5 mins by Fluo4-AM dye-based fluorescence assay	[PMID: 28740604]
CHO	EC₅₀	95 3 Compound: Menthol	Agonist activity at mouse TRPA1 expressed in CHO cells assessed as increase in calcium influx by patch clamp electrophysiology method Agonist activity at mouse TRPA1 expressed in CHO cells assessed as increase in calcium influx by patch clamp electrophysiology method	[PMID: 30878828]
HEK293	EC₅₀	96 3 Compound: L-Menthol	Agonist activity at recombinant human TRPA1 expressed in HEK293 cells assessed as increase in intracellular Ca2+ level pre-incubated for 5 mins by Fluo4-AM dye-based fluorescence assay Agonist activity at recombinant human TRPA1 expressed in HEK293 cells assessed as increase in intracellular Ca2+ level pre-incubated for 5 mins by Fluo4-AM dye-based fluorescence assay	[PMID: 28740604]
CHO	EC₅₀	56 3 Compound: 27	Antagonist activity at mouse TRPA1 channel expressed in CHO cells assessed as blockade of agonist-induced whole cell conductance by electrophysiology Antagonist activity at mouse TRPA1 channel expressed in CHO cells assessed as blockade of agonist-induced whole cell conductance by electrophysiology	[PMID: 20356305]
HEK293	EC₅₀	10.1 3 Compound: L-Menthol	Agonist activity at full length recombinant human TRPM8 expressed in HEK293 cells assessed as increase in intracellular Ca2+ level pre-incubated for 5 mins by Fluo4-AM dye-based fluorescence assay Agonist activity at full length recombinant human TRPM8 expressed in HEK293 cells assessed as increase in intracellular Ca2+ level pre-incubated for 5 mins by Fluo4-AM dye-based fluorescence assay	[PMID: 28740604]
HEK293	EC₅₀	96 3 Compound: L-Menthol	Agonist activity at recombinant human TRPA1 expressed in HEK293 cells assessed as increase in intracellular Ca2+ level pre-incubated for 5 mins by Fluo4-AM dye-based fluorescence assay Agonist activity at recombinant human TRPA1 expressed in HEK293 cells assessed as increase in intracellular Ca2+ level pre-incubated for 5 mins by Fluo4-AM dye-based fluorescence assay	[PMID: 28740604]
HEK293	EC₅₀	29 3 Compound: Menthol	Activity at human TRPM8 expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation Activity at human TRPM8 expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation	[PMID: 17293875]
HEK293	EC₅₀	34.1 3 Compound: Menthol	Activity at human TRPM8 H845A mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation Activity at human TRPM8 H845A mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation	[PMID: 17293875]
HEK293	EC₅₀	11.7 3 Compound: Menthol	Activity at human TRPM8 K856A mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation Activity at human TRPM8 K856A mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation	[PMID: 17293875]
HEK293	EC₅₀	35.3 3 Compound: Menthol	Activity at human TRPM8 K856R mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation Activity at human TRPM8 K856R mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation	[PMID: 17293875]
HEK293	EC₅₀	818 3 Compound: Menthol	Activity at human TRPM8 R842A mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation Activity at human TRPM8 R842A mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation	[PMID: 17293875]
HEK293	EC₅₀	93 3 Compound: Menthol	Activity at human TRPM8 R842K mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation Activity at human TRPM8 R842K mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation	[PMID: 17293875]
HEK293	EC₅₀	38.4 3 Compound: Menthol	Activity at human TRPM8 R851Q mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation Activity at human TRPM8 R851Q mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation	[PMID: 17293875]
HEK293	EC₅₀	38.8 3 Compound: Menthol	Activity at human TRPM8 R862A mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation Activity at human TRPM8 R862A mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation	[PMID: 17293875]
HEK293	EC₅₀	477 3 Compound: Menthol	Activity at human TRPM8 S4b_M mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation Activity at human TRPM8 S4b_M mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation	[PMID: 17293875]
HEK293	EC₅₀	30 3 Compound: Menthol	Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by Fluo-4-AM dye based fluorescence assay Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by Fluo-4-AM dye based fluorescence assay	[PMID: 30878828]
HEK293	EC₅₀	75 3 Compound: Menthol	Agonist activity at rat TRPM8 expressed in HEK293 cells assessed as increase in current amplitude at +80 mV holding potential by whole cell patch-clamp assay Agonist activity at rat TRPM8 expressed in HEK293 cells assessed as increase in current amplitude at +80 mV holding potential by whole cell patch-clamp assay	[PMID: 26847872]
HEK293	EC₅₀	3 3 Compound: Menthol	Inhibition of human TRPM8 (unknown origin) expressed in HEK293 cells assessed as inhibition of WS12-induced Ca2+ elevation by FLIPR assay Inhibition of human TRPM8 (unknown origin) expressed in HEK293 cells assessed as inhibition of WS12-induced Ca2+ elevation by FLIPR assay	[PMID: 24080460]
HEK293	IC₅₀	92 3 Compound: Menthol	Displacement of [3H]menthol from human TRPM8 expressed in HEK293 cell membranes by scintillation counting Displacement of [3H]menthol from human TRPM8 expressed in HEK293 cell membranes by scintillation counting	[PMID: 17293875]
HEK293	IC₅₀	19 3 Compound: Menthol	Displacement of [3H]menthol from human TRPM8 K856A mutant expressed in HEK293 cell membranes by scintillation counting Displacement of [3H]menthol from human TRPM8 K856A mutant expressed in HEK293 cell membranes by scintillation counting	[PMID: 17293875]
HEK293	IC₅₀	520 3 Compound: Menthol	Displacement of [3H]menthol from human TRPM8 R842A mutant expressed in HEK293 cell membranes by scintillation counting Displacement of [3H]menthol from human TRPM8 R842A mutant expressed in HEK293 cell membranes by scintillation counting	[PMID: 17293875]

In Vitro

(-)-Menthol (Menthol ) per se does not exhibit antiproliferative activity, but it is able to enhance 1α,25(OH)₂D₃-mediated growth inhibition in LNCaP cells. At high (-)-Menthol concentrations above 1.6 mM, the cells begin to detach from the culture dish^[1].
(-)-Menthol (0.8 mM) can increase [Ca²⁺]i via transmembrane influx or store release pathways. Peak increase in [Ca²⁺]_i was 102.3±39 nM (n=3) in (-)-Menthol alone and 124.5±51 nM (n=3) in combination of 1α,25(OH)₂D₃ with (-)-Menthol, respectively^[1].
Combination of 1α,25(OH)₂D₃ with (-)-Menthol (0.8 mM) cooperatively modulates bcl-2 and p21 expression^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Masse moléculaire

156.27

Formule

C₁₀H₂₀O

CAS No.

2216-51-5

Appearance

<41°C Solid,>44°C Liquid

Color

Colorless to off-white

SMILES

O[C@H]1[C@H](C(C)C)CC[C@@H](C)C1

Structure Classification

Initial Source

Endogenous metabolite

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (639.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1.89 mg/mL (12.09 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.3992 mL	31.9959 mL	63.9918 mL
5 mM	1.2798 mL	6.3992 mL	12.7984 mL
10 mM	0.6399 mL	3.1996 mL	6.3992 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculateur de molarité
Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (16.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (16.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (16.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureté et documentation

Purity: 99.79%

Select Batch:

Fiche technique (274 KB) SDS (393 KB)

COA (248 KB) HNMR (116 KB) GC (232 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Park EJ, et al. Menthol Enhances an Antiproliferative Activity of 1alpha,25-Dihydroxyvitamin D(3) in LNCaP Cells. J Clin Biochem Nutr. 2009 Mar;44(2):125-30. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	6.3992 mL	31.9959 mL	63.9918 mL	159.9795 mL
	5 mM	1.2798 mL	6.3992 mL	12.7984 mL	31.9959 mL
	10 mM	0.6399 mL	3.1996 mL	6.3992 mL	15.9980 mL
DMSO	15 mM	0.4266 mL	2.1331 mL	4.2661 mL	10.6653 mL
	20 mM	0.3200 mL	1.5998 mL	3.1996 mL	7.9990 mL
	25 mM	0.2560 mL	1.2798 mL	2.5597 mL	6.3992 mL
	30 mM	0.2133 mL	1.0665 mL	2.1331 mL	5.3327 mL
	40 mM	0.1600 mL	0.7999 mL	1.5998 mL	3.9995 mL
	50 mM	0.1280 mL	0.6399 mL	1.2798 mL	3.1996 mL
	60 mM	0.1067 mL	0.5333 mL	1.0665 mL	2.6663 mL
	80 mM	0.0800 mL	0.3999 mL	0.7999 mL	1.9997 mL
	100 mM	0.0640 mL	0.3200 mL	0.6399 mL	1.5998 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

(-)-Menthol Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(-)-Menthol2216-51-5TRP ChannelEnvironmental PollutantsEndogenous MetaboliteTransient receptor potential channelsEnvironmental ContaminantsInhibitorinhibitorinhibit

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(-)-Menthol

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Other Forms of (-)-Menthol:

(-)-Menthol Related Antibodies

4 Publications Citing Use of MCE (-)-Menthol

Recommandations en vedette

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Voir tous les produits spécifiques à Isoform TRP Channel:

Voir tous les produits spécifiques à Isoform Endogenous Metabolite:

Activité biologique

Pureté et documentation

Références

Avis client

(-)-Menthol Related Antibodies

Calculateur de molarité

Calculateur de dilution

Complete Stock Solution Preparation Table

(-)-Menthol Related Classifications

Keywords:

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