Ginsenoside Rh1 (Synonyms: Prosapogenin A2; Sanchinoside B2; Sanchinoside Rh1)

Name: Ginsenoside Rh1
Brand: MedChemExpress
SKU: HY-N0604
Rating: 5.0 (5 reviews)

Cat. No.: HY-N0604 Purity: 98.72%: Data Sheet
COA

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Ginsenoside Rh1 (Prosapogenin A2) inhibits the expression of PPAR-γ, TNF-α, IL-6, and IL-1β.

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CAS No. : 63223-86-9

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

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고객리뷰

Based on 5 publication(s) in Google Scholar

Other Forms of Ginsenoside Rh1:

(20R)-Ginsenoside Rh1 In-stock
Ginsenoside Rh1 (Standard) 견적 받기

5 Publications Citing Use of MCE Ginsenoside Rh1

Histological Imaging/Staining

Cell Proliferation/Viability Assay

RT-PCR

: Ginsenoside Rh1 purchased from MedChemExpress. Usage Cited in: Food Sci Biotechnol. 2023 Aug 18;33(5):1233-1243.; The cell viability of C2C12 myotubes was assessed in the presence of Ginsenoside Rh1 (0.5, 1, 2 μM).

: Ginsenoside Rh1 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2021 Feb;37(1):65-84. [Abstract]; H&E staining of liver tissues showed there was a significant reduction of hepatic lipid droplets and lipid accumulation was improved in Ginsenoside Rh1 (20 mg/kg, p.o.)-treated mice.

: Ginsenoside Rh1 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2021 Feb;37(1):65-84. [Abstract]; mRNA expression levels of PPARγ and HNF1b in Ginsenoside Rh1 (20 mg/kg, p.o.)-treated mice was determined in the liver tissues of male and female mice using qRT-PCR

: Ginsenoside Rh1 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2021 Feb;37(1):65-84. [Abstract]; Protein expression levels of PPARγ and HNF1b in Ginsenoside Rh1 (20 mg/kg, p.o.)-treated mice was determined in the liver tissues of male and female mice using immunoblotting.

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Biological Activity
Protocol
순도&문서
References
고객리뷰

제품 설명

Ginsenoside Rh1 (Prosapogenin A2) inhibits the expression of PPAR-γ, TNF-α, IL-6, and IL-1β.

IC₅₀ & Target^[1]

PPAR-γ

IL-6

IL-1β

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	> 200 3 Compound: 11	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 26420067]
Hepatocyte	IC₅₀	105 3 Compound: 9	Hepatoprotective activity in mouse hepatocytes assessed inhibition of D-galactosamine/TNFalpha-induced cell death dosed administered before 30 mins of TNFalpha challenge measured after 18 hrs Hepatoprotective activity in mouse hepatocytes assessed inhibition of D-galactosamine/TNFalpha-induced cell death dosed administered before 30 mins of TNFalpha challenge measured after 18 hrs	[PMID: 11325227]
Hepatocyte	IC₅₀	105 3 Compound: 9	Hepatoprotective activity in mouse hepatocytes assessed inhibition of D-galactosamine/TNFalpha-induced cell death dosed administered before 30 mins of TNFalpha challenge measured after 18 hrs Hepatoprotective activity in mouse hepatocytes assessed inhibition of D-galactosamine/TNFalpha-induced cell death dosed administered before 30 mins of TNFalpha challenge measured after 18 hrs	[PMID: 11325227]
HepG2	IC₅₀	> 200 3 Compound: 11	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 26420067]
HepG2	IC₅₀	>200 3 Compound: 11	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 26420067]
HepG2	IC₅₀	> 200 3 Compound: 11	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 26420067]
HL-60	IC₅₀	> 200 3 Compound: 11	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 26420067]
HL-60	IC₅₀	>200 3 Compound: 11	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 26420067]
Hepatocyte	IC₅₀	105 3 Compound: 9	Hepatoprotective activity in mouse hepatocytes assessed inhibition of D-galactosamine/TNFalpha-induced cell death dosed administered before 30 mins of TNFalpha challenge measured after 18 hrs Hepatoprotective activity in mouse hepatocytes assessed inhibition of D-galactosamine/TNFalpha-induced cell death dosed administered before 30 mins of TNFalpha challenge measured after 18 hrs	[PMID: 11325227]

In Vitro

The effect of Ginsenoside Rh1 is examined on adipogenesis in 3T3-L1 cells. Ginsenoside Rh1 potently inhibits the adipogenesis, as assessed by Oil-red O staining and lipid contents in 3T3-L1 adipocytes. Ginsenoside Rh1, at concentrations of 50 μM and 100 μM, inhibit the adipogenesis by 50% and 63%, respectively.The expression levels of adipocytespecific genes such as PPAR-γ, C/EBP-α, FAS, aFABP and some genes are examined during early phase of differentiation such as Pref-1, C/EBP-δ and Glucocorticoid receptor (GR). After the treatment with Ginsenoside Rh1 in 3T3-L1 cells, mRNA is extracted on 18 h and 24 h for Pref-1, C/EBP-δ and GR and day 8 for PPAR-γ, C/EBP-α, FAS, aFABP. Then, the expression profiles of adipocyte-specific genes are investigated by RT-PCR. PPAR-γ, C/EBP-α, FAS, and aFABP expressions are significantly increased in DMI-stimulated differentiated adipocyte compared to those of non-stimulated adipocyte cells. However, treatment with DMI in the presence of Ginsenoside Rh1 significantly suppresses the expression levels of PPAR-γ, C/EBP-α, FAS, and aFABP in a dose- dependent manner, whereas the expression levels of Pref-1, C/EBP-δ and GR are not affected^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

When high-fat diet (HFD) fed mice for 8 weeks, body and epididymal fat weight gains are significantly increased compared to those of low-fat diet (LFD)-fed mice. However, when Ginsenoside Rh1 is treated in HFD-fed mice, body and epididymal fat weight gains are significantly decrease compared with those of the HFD-fed mice. TG, glucose, insulin, total cholesterol, and HDL levels in the blood are significantly increased in HFD-fed mice group compared to LFD-fed mice group. Treatment with Ginsenoside Rh1 in HFD-fed mice significantly lowers TG level alone^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

638.87

화학식

C₃₆H₆₂O₉

CAS No.

63223-86-9

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]([C@@]12C)(C[C@H](O[C@]([C@@H]([C@@H](O)[C@@H]3O)O)([H])O[C@@H]3CO)[C@@]4([H])C5(C)C)[C@@](C[C@@H](O)[C@]1([H])[C@]([C@@](C)(O)CC/C=C(C)/C)([H])CC2)([H])[C@]4(CC[C@@H]5O)C

Structure Classification

Initial Source

Endogenous metabolite

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

용액&용해도

In Vitro:

DMSO : 100 mg/mL (156.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5653 mL	7.8263 mL	15.6526 mL
5 mM	0.3131 mL	1.5653 mL	3.1305 mL
10 mM	0.1565 mL	0.7826 mL	1.5653 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 98.72%

Select Batch:

Data Sheet (284 KB) SDS (393 KB)

COA (259 KB) HNMR (322 KB) RP-HPLC (79 KB) MS (70 KB)

Handling Instructions (2659 KB)

References

[1]. Gu W, et al. Ginsenoside Rh1 ameliorates high fat diet-induced obesity in mice by inhibiting adipocyte differentiation. Biol Pharm Bull. 2013;36(1):102-7. [Content Brief]

Cell Assay ^[1]	Mouse embryo fibroblasts 3T3-L1 cells are incubated in DMEM, containing 10% FBS and 1% AB, at 37°C and 5.6% CO2 atmosphere. To induce differentiation, two days after confluence, preadipocytes (designated day 0) are cultured in the differentiation medium (DM), which is consisted of DMEM, 10% FBS, 1% AB, and DMI (0.28 unit/mL insulin, 0.5 mM Isobutylmethylxanthine and 1 μM Dexamethasone) for 2 d in the presence or absence of 50 μM and 100 μM of Ginsenoside Rh1, and switched to DM containing 10% FBS and 10 μg/mL insulin and then changed to DMEM medium with 10% FBS for every 2 d^[1]. MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.
Animal Administration ^[1]	Mice^[1] Male C57BL/6J mice are separated into 3 groups, LFD, HFD and HFDRh1. Each group is consisted of ten mice. LFD group fed LFD for 8 weeks. HFD group fed HFD for 8 weeks. HFDRh1 group fed HFD diet for 4 weeks and then simultaneously treated with HFD and 20 mg/kg/d Ginsenoside Rh1, which is orally administrated. Weight and food intake of mice are measured daily. After finishing treatment for 4 weeks, blood and epididymal fats are collected for further analysis. MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.
References	[1]. Gu W, et al. Ginsenoside Rh1 ameliorates high fat diet-induced obesity in mice by inhibiting adipocyte differentiation. Biol Pharm Bull. 2013;36(1):102-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.5653 mL	7.8263 mL	15.6526 mL	39.1316 mL
	5 mM	0.3131 mL	1.5653 mL	3.1305 mL	7.8263 mL
	10 mM	0.1565 mL	0.7826 mL	1.5653 mL	3.9132 mL
	15 mM	0.1044 mL	0.5218 mL	1.0435 mL	2.6088 mL
	20 mM	0.0783 mL	0.3913 mL	0.7826 mL	1.9566 mL
	25 mM	0.0626 mL	0.3131 mL	0.6261 mL	1.5653 mL
	30 mM	0.0522 mL	0.2609 mL	0.5218 mL	1.3044 mL
	40 mM	0.0391 mL	0.1957 mL	0.3913 mL	0.9783 mL
	50 mM	0.0313 mL	0.1565 mL	0.3131 mL	0.7826 mL
	60 mM	0.0261 mL	0.1304 mL	0.2609 mL	0.6522 mL
	80 mM	0.0196 mL	0.0978 mL	0.1957 mL	0.4891 mL
	100 mM	0.0157 mL	0.0783 mL	0.1565 mL	0.3913 mL

Ginsenoside Rh1 Related Classifications

Inflammation/Immunology

Help & FAQs

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비고

Ginsenoside Rh1 (Synonyms: Prosapogenin A2; Sanchinoside B2; Sanchinoside Rh1)

고객리뷰

Other Forms of Ginsenoside Rh1:

5 Publications Citing Use of MCE Ginsenoside Rh1

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Biological Activity

Protocol

순도&문서

References

고객리뷰

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

Ginsenoside Rh1 Related Classifications

Keywords:

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Ginsenoside Rh1 (Synonyms: Prosapogenin A2; Sanchinoside B2; Sanchinoside Rh1)

고객리뷰

Other Forms of Ginsenoside Rh1:

Ginsenoside Rh1 Related Antibodies

5 Publications Citing Use of MCE Ginsenoside Rh1

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All PPAR Isoform Specific Products:

View All TNF Receptor Isoform Specific Products:

View All Interleukin Related Isoform Specific Products:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Protocol

순도&문서

References

고객리뷰

Ginsenoside Rh1 Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

Ginsenoside Rh1 Related Classifications

Keywords:

최근 본 상품:

고객리뷰

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SAFETY DATA SHEET (SDS)

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