(20R)-Ginsenoside Rh1
Based on 1 Customer Validation
(20R)-Ginsenoside Rh1 is the R-isomer of Ginsenoside Rh1 (HY-N0604), and it is found in red ginseng, ginseng, and other sources. (20R)-Ginsenoside Rh1 inhibits thrombin-induced conversion of fibrinogen to fibrin, exerting anticoagulant and antithrombotic effects.
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- Pureté : 99.94%
- CAS No.: 80952-71-2
- Formule: C36H62O9
- Masse moléculaire:638.87
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Stockage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
>10 μM
Compound: 21
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Cytotoxicity against human A549 cells
Cytotoxicity against human A549 cells
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[PMID: 26200131] |
| HepG2 | IC50 |
>200 μM
Compound: 12
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Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
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[PMID: 26420067] |
| HL-60 | IC50 |
>10 μM
Compound: 21
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Cytotoxicity against human HL60 cells
Cytotoxicity against human HL60 cells
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[PMID: 26200131] |
| HL-60 | IC50 |
>200 μM
Compound: 12
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Cytotoxicity against human HL60 cells after 48 hrs by MTT assay
Cytotoxicity against human HL60 cells after 48 hrs by MTT assay
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[PMID: 26420067] |
| MCF7 | IC50 |
>10 μM
Compound: 21
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Cytotoxicity against human MCF7 cells
Cytotoxicity against human MCF7 cells
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[PMID: 26200131] |
| SW480 | IC50 |
>10 μM
Compound: 21
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Cytotoxicity against human SW480 cells
Cytotoxicity against human SW480 cells
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[PMID: 26200131] |
In Vitro
(20R)-Ginsenoside Rh1 exhibits greater inhibitory activity on human blood platelet aggregation induced by collagen or ADP compared to 20(S)-Ginsenoside Rh1[2].
(20R)-Ginsenoside Rh1 (10 µM) significantly inhibits gap junction intercellular communication via activation of both tyrosine kinase and protein kinase C[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 80952-71-2
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Appearance Solid
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Masse moléculaire 638.87
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Formule C36H62O9
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Color White to off-white
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SMILES
O[C@H]([C@H]([C@@H]([C@@H](CO)O1)O)O)[C@@H]1O[C@@H]2[C@@]3([H])C(C)(C)[C@@H](O)CC[C@]3(C)[C@@]4([H])C[C@@H](O)[C@@H]5[C@@H]([C@](C)(O)CC/C=C(C)\C)CC[C@](C)5[C@@](C)4C2
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (156.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 10 mg/mL (15.65 mM); Clear solution
This protocol yields a clear solution of ≥ 10 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 10 mg/mL (15.65 mM); Clear solution
This protocol yields a clear solution of ≥ 10 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (277 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Fan X, et al. Pharmacokinetic Comparison of 20(R)- and 20(S)-Ginsenoside Rh1 and 20(R)- and 20(S)-Ginsenoside Rg3 in Rat Plasma following Oral Administration of Radix Ginseng Rubra and Sheng-Mai-San Extracts. Evid Based Complement Alternat Med. 2017;2017:6451963. [Content Brief]
[2]. Tam DNH, et al. Ginsenoside Rh1: A Systematic Review of Its Pharmacological Properties. Planta Med. 2018;84(3):139-152. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5653 mL | 7.8263 mL | 15.6526 mL | 39.1316 mL |
| 5 mM | 0.3131 mL | 1.5653 mL | 3.1305 mL | 7.8263 mL | |
| 10 mM | 0.1565 mL | 0.7826 mL | 1.5653 mL | 3.9132 mL | |
| 15 mM | 0.1044 mL | 0.5218 mL | 1.0435 mL | 2.6088 mL | |
| 20 mM | 0.0783 mL | 0.3913 mL | 0.7826 mL | 1.9566 mL | |
| 25 mM | 0.0626 mL | 0.3131 mL | 0.6261 mL | 1.5653 mL | |
| 30 mM | 0.0522 mL | 0.2609 mL | 0.5218 mL | 1.3044 mL | |
| 40 mM | 0.0391 mL | 0.1957 mL | 0.3913 mL | 0.9783 mL | |
| 50 mM | 0.0313 mL | 0.1565 mL | 0.3131 mL | 0.7826 mL | |
| 60 mM | 0.0261 mL | 0.1304 mL | 0.2609 mL | 0.6522 mL | |
| 80 mM | 0.0196 mL | 0.0978 mL | 0.1957 mL | 0.4891 mL | |
| 100 mM | 0.0157 mL | 0.0783 mL | 0.1565 mL | 0.3913 mL |
(20R)-Ginsenoside Rh1 Related Classifications
HY-N1400 Related Classifications
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