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  3. Phorbol 12-myristate 13-acetate

Phorbol 12-myristate 13-acetate (Synonyms: PMA)

製品番号: HY-18739 純度: 99.66%
取扱説明書

Phorbol 12-myristate 13-acetate (PMA), a phorbol ester, is a dual SphK and protein kinase C (PKC) activator.

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Phorbol 12-myristate 13-acetate 構造式

Phorbol 12-myristate 13-acetate 構造式

CAS 番号 : 16561-29-8

容量 価格(税別) 在庫状況 数量
10 mM * 1  mL in DMSO USD 147 在庫あり
Estimated Time of Arrival: December 31
1 mg USD 60 在庫あり
Estimated Time of Arrival: December 31
5 mg USD 108 在庫あり
Estimated Time of Arrival: December 31
10 mg USD 156 在庫あり
Estimated Time of Arrival: December 31
25 mg USD 280 在庫あり
Estimated Time of Arrival: December 31
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100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

カスタマーレビュー

Based on 42 publication(s) in Google Scholar

Top Publications Citing Use of Products

顧客検証

    Phorbol 12-myristate 13-acetate purchased from MCE. Usage Cited in: Front Mol Neurosci. 2017 Aug 7;10:247.

    Bar graphs show levels of GABAAR-α2 mRNA and GABAAR-α2 protein in BLA of control mice treated with BLA-injection of H89 or GF109203X (GFX); in MPTP-mice treated with BLA-injection of PMA, or the co-administration of quinpirole and H89 (quin/+H89) or GF109203X (quin/+GFX).

    Phorbol 12-myristate 13-acetate purchased from MCE. Usage Cited in: Pharmacol Res. 2019 Apr;142:1-13.

    Total lysates from cells are analyzed for the expression of MMP-2 and MMP-9 by Western blot analysis in the treatment of different concentrations of PMA and PDD.

    Phorbol 12-myristate 13-acetate purchased from MCE. Usage Cited in: Pharmacol Res. 2019 Apr;142:1-13.

    MDA-MB-231 cells are pretreated with PPD for 24 h followed by exposure to 50 ng/mL of PMA for 30 min. The whole cell lysates are analyzed by Western blot for the activation of MAPK.
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    製品説明

    Phorbol 12-myristate 13-acetate (PMA), a phorbol ester, is a dual SphK and protein kinase C (PKC) activator[1].

    IC50 & Target

    PKC

    11.7 nM (EC50)

    体外実験

    In order to examine the role of PKC in p38MAPK phosphorylation, the cells are stimulated with the PKC activator, PMA (100 nM), which mimics the binding of DAG, the natural activator of PKC, to the C1 region of the PKCs. p38MAPK phosphorylation by PMA is observed in the two cell types similar to that observed by GnRH in αT3-1 cells, that is, a slow sustained activation (3.2-fold and 3.6-fold, respectively at 30 min). The paradoxical findings that PKCs activated by GnRH and PMA play a differential role in p38MAPK phosphorylation may be explained by differential localization of the PKCs. Basal, GnRH- and PMA- stimulation of p38MAPK phosphorylation in αT3-1 cells is mediated by Ca2+ influx via voltage-gated Ca2+ channels and Ca2+ mobilization, while in the differentiated LβT2 gonadotrope cells it is mediated only by Ca2+ mobilization[2].

    体内実験

    PMA is a PKC agonist, which reverses the damage induced by 5-hydroxydecanoic acid (5-HD). Thus, activation of the mitoKATP protected mitochondrial function in SOD and MDA via the PKC pathway[3].

    分子量

    616.83

    分子式

    C₃₆H₅₆O₈

    CAS 番号

    16561-29-8

    SMILES

    CCCCCCCCCCCCCC(O[[email protected]]([[email protected]]1C)[[email protected]]2(OC(C)=O)[[email protected]@]([[email protected]@](C=C(CO)C[[email protected]]34O)([H])[[email protected]@]1(O)[[email protected]]4([H])C=C(C)C3=O)([H])C2(C)C)=O

    輸送条件

    Room temperature in continental US; may vary elsewhere.

    保管条件

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    溶剤 & 溶解度
    体外: 

    Ethanol : 100 mg/mL (162.12 mM; Need ultrasonic)

    DMSO : ≥ 50 mg/mL (81.06 mM)

    H2O : < 0.1 mg/mL (insoluble)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6212 mL 8.1060 mL 16.2119 mL
    5 mM 0.3242 mL 1.6212 mL 3.2424 mL
    10 mM 0.1621 mL 0.8106 mL 1.6212 mL
    *Please refer to the solubility information to select the appropriate solvent.
    体内:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (4.05 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 2.5 mg/mL (4.05 mM); Suspended solution; Need ultrasonic

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (4.05 mM); Clear solution

    • 4.

      Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (4.05 mM); Clear solution

    • 5.

      Add each solvent one by one:  10% EtOH    90% (20% SBE-β-CD in saline)

      Solubility: 2.5 mg/mL (4.05 mM); Suspended solution; Need ultrasonic

    • 6.

      Add each solvent one by one:  10% EtOH    90% corn oil

      Solubility: ≥ 2.5 mg/mL (4.05 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    参考文献
    細胞実験
    [2]

    αT3-1 and LβT-2 cells are grown in monolayer cultured in DMEM in humidified incubator 5% CO2 at 37°C. Serum starvation is with 0.1% FCS in the same medium for 16 h. GnRH and PMA are then added for the length of time as indicated. In general, αT3-1 cells are transiently transfected by ExGen 500 or by jetPRIME, while LβT2 cells only by jetPRIME transfection reagent. For experiments with dominant-negative (DN) PKCs, αT3-1 cells (in 6 cm plates) are transfected with 1.5 μg of p38α-GFP with 3 μg of control vector, pCDNA3, or with 3 μg of the DN-PKCs constructs. For LβT2 cells, transfections are performed (in 10 cm plates) with 4 μg of p38α-GFP along with 9 μg of control vector, pCDNA3, or with 9 μg of the DN-PKCs constructs. Approximately 30 h after transfection, the cells are serum starved (0.1% FCS) for 16 h and later stimulated with GnRH or PMA, washed twice with ice-cold PBS, treated with the lysis buffer, followed by one freeze-thaw cycle. Cells are harvested; following centrifugation (15,000×g, 15 min, 4°C) supernatants are taken for immunoprecipitation experiments[2].

    MCE はこれらの方法の精度を確認していません。 こちらは参照専用です。

    動物実験
    [3]

    Rats[3]
    All experiments qre performed with male Wistar rats (weighing 250-280 g). One hundred and thirty-five Wistar rats are randomly divided into seven groups. (1) Rats in the sham group (n=21) are given a lateral cerebral ventricle injection of 0.9% normal saline; (2) Rats in the IR group (n=21) are given a lateral cerebral ventricle injection of 0.9% normal saline 30 min before middle cerebral artery occlusion (MCAO); (3) Rats in the Carbenoxolone (CBX) group (n=21) are given a lateral cerebral ventricle injection of CBX (5 μg/mL×10 μL) 30 min before MCAO; (4) Rats in the Sch-6783 group (n=21) are given a lateral cerebral ventricle injection of DZX (2 mM×30 μL) 30 min prior to MCAO; (5) Rats in the 5-HD group (n=21) are given a lateral cerebral ventricle injection of 5-HD (100 mM×10 μL), and after 10 min, DZX is injected 15 min prior to MCAO; (6) The rats in the DZX + Ro group (n=15) are given a lateral cerebral ventricle injection of DZX, and after 10 min, Ro-31-8425 (400 μg/kg) is injected 15 min prior to MCAO; (7) The rats in the 5-HD+PMA group (n=15) are given an intraperitoneal injection of PMA (200 μg/kg) after the injection of 5-HD and DZX.

    MCE はこれらの方法の精度を確認していません。 こちらは参照専用です。

    参考文献

    純度: 99.66%

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    Keywords:

    Phorbol 12-myristate 13-acetatePMAPKCSPHKProtein kinase CSphingosine kinaseInhibitorinhibitorinhibit

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    製品名:
    Phorbol 12-myristate 13-acetate
    製品番号:
    HY-18739
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