Novobiocin sodium (Synonyms: Albamycin sodium; Cathomycin sodium)

Name: Novobiocin sodium
Brand: MedChemExpress
SKU: HY-B0425A
Rating: 5.0 (12 reviews)

Cat. No.: HY-B0425A Purity: 99.07%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Novobiocin (Albamycin) sodium is a potent and orally active antibiotic. Novobiocin sodium also is a DNA gyrase inhibitor and a heat shock protein 90 (Hsp90) antagonist. Novobiocin sodium has the potential for the research of highly beta-lactam-resistant pneumococcal infections. Novobiocin sodium shows anti-orthopoxvirus activity.

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CAS No. : 1476-53-5

사이즈	재고	수량

무료 샘플 (0.1 - 0.2 mg)	지금 신청하기
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
100 mg	해외재고보유	Estimated Time of Arrival: December 31
500 mg	해외재고보유	Estimated Time of Arrival: December 31
1 g	해외재고보유	Estimated Time of Arrival: December 31
5 g	견적 받기
10 g	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 12 publication(s) in Google Scholar

Other Forms of Novobiocin sodium:

Novobiocin In-stock

12 Publications Citing Use of MCE Novobiocin sodium

Bio/Physico-chemical Assay

Cell Imaging/Staining

Cell Proliferation/Viability Assay

: Novobiocin sodium purchased from MedChemExpress. Usage Cited in: J Med Virol. 2025 Oct;97(10):e70655. [Abstract]; Novobiocin also reduced NP expression at 25 μM, with mean fluorescence intensities significantly lower than those of the untreated control.

: Novobiocin sodium purchased from MedChemExpress. Usage Cited in: J Med Virol. 2025 Oct;97(10):e70655. [Abstract]; Cytoxicity and inhibition of Novobiocin, EC50 = 25.12 μM.

: Novobiocin sodium purchased from MedChemExpress. Usage Cited in: Nat Methods. 2023 Sep;20(9):1388-1399. [Abstract]; Editing efficiencies of the VCAN target using Cas9D10A double nicking in H9 hESCs carrying the K3753R mutation and additional small molecules to inhibit the MMEJ repair proteins Polϴ, PARP or Ligase I/III: Novobiocin.

: Novobiocin sodium purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2019 Mar 5;20(5). pii: E1125. [Abstract]; Cell viability assay to analyze the impact of Probenecid and Novobiocin treatments on resistance.

: Novobiocin sodium purchased from MedChemExpress. Usage Cited in: Blood. 2018 Jul 19;132(3):307-320. [Abstract]; K562, KCL22 and HL60 are treated with the indicated (cytotoxic) concentration of Amidopyrine (AX), Novobiocin (NB) and AUY922 for 48h and later protein lysates are subjected to immunoblot analysis.

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Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

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RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

View All HSP Isoform Specific Products:

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HSP40 HSP70 HSP90 HSP105 HSF1 HSP HSPA5

Biological Activity
순도&문서
References
고객리뷰

제품 설명

IC₅₀ & Target^[1]

β-lactam

HSP90

In Vitro

Novobiocin sodium (1 mM) competitively inhibits ATP binding to gyrase B to interfere with nucleotide binding and interferes with the association of the co-chaperones Hsc70 and p23 with Hsp90^[1].
Novobiocin sodium (200 μM; 24 h) inhibits the rate of repair of both cis-DDP and BCNU induced DNA interstrand cross-links and with a corresponding decrease in the clonogenic survival of the human glioblastoma multiforme cells^[2].
Novobiocin sodium (0.3 mM; 48 hours) induces a caspase-3/7 enzyme–dependent apoptosis assays with an induction of approximately three- to fivefold of apoptotic cells in K562, HL60, Mutz-2^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	K562, HL60, Mutz-2 cells
Concentration:	0.3 mM
Incubation Time:	48 hours
Result:	Decreased caspase-3/7 activity in K562, HL60, Mutz-2 cells.

In Vivo

Novobiocin sodium (25, 50, 100, 200 mg/kg; s.c.; 4 times at 1, 5, 24 and 48 h after infection) shows anti-infection activity in mice infected with amoxicillin-resistant Streptococcus pneumoniae^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	30 g adult female Swiss mice (sepsis induced by the penicillin-susceptible strain (AR33118))^[3]
Dosage:	25, 50, 100, 200 mg/kg
Administration:	S.c.; given at 1, 5, 24 and 48 h after infection
Result:	Showed anti-infection activity in mice infected with amoxicillin-resistant S. pneumoniae.

분자량

634.61

화학식

C₃₁H₃₅N₂NaO₁₁

CAS No.

1476-53-5

Appearance

Solid

Color

White to off-white

SMILES

CC1=C(OC2=O)C(C(O)=C2NC(C3=CC(C/C=C(C)/C)=C(O[Na])C=C3)=O)=CC=C1O[C@H](OC(C)(C)[C@H](OC)[C@H]4OC(N)=O)[C@@H]4O

Structure Classification

Initial Source

Microorganisms

Streptomyces niveus

선적

Room temperature in continental US; may vary elsewhere.

보관

4°C, sealed storage, away from moisture

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

용액&용해도

In Vitro:

DMSO : ≥ 100 mg/mL (157.58 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (78.79 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5758 mL	7.8789 mL	15.7577 mL
5 mM	0.3152 mL	1.5758 mL	3.1515 mL
10 mM	0.1576 mL	0.7879 mL	1.5758 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (157.58 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

순도&문서

Purity: 99.12%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (249 KB) HNMR (176 KB) LCMS (105 KB)

Handling Instructions (2659 KB)

References

[1]. Marcu MG, et al. The heat shock protein 90 antagonist novobiocin interacts with a previously unrecognized ATP-binding domain in the carboxyl terminus of the chaperone. J Biol Chem. 2000 Nov 24;275(47):37181-6. [Content Brief]

[2]. Ali-Osman F, et al. Topoisomerase II inhibition and altered kinetics of formation and repair of nitrosourea and cisplatin-induced DNA interstrand cross-links and cytotoxicity in human glioblastoma cells. Cancer Res. 1993 Dec 1;53(23):5663-8. [Content Brief]

[3]. Rodríguez-Cerrato V, et al. Comparative efficacy of novobiocin and amoxicillin in experimental sepsis caused by beta-lactam-susceptible and highly resistant pneumococci. Int J Antimicrob Agents. 2010 Jun;35(6):544-9. [Content Brief]

[4]. Eder JP, et al. A phase I clinical trial of novobiocin, a modulator of alkylating agent cytotoxicity. Cancer Res. 1991 Jan 15;51(2):510-3. [Content Brief]

[5]. Bhatia S, et al. Targeting HSP90 dimerization via the C terminus is effective in imatinib-resistant CML and lacks the heat shock response.Blood. 2018 Jul 19;132(3):307-320. [Content Brief]

[6]. Smee DF. Progress in the discovery of compounds inhibiting orthopoxviruses in animal models. Antivir Chem Chemother. 2008;19(3):115-24. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.5758 mL	7.8789 mL	15.7577 mL	39.3943 mL
	5 mM	0.3152 mL	1.5758 mL	3.1515 mL	7.8789 mL
	10 mM	0.1576 mL	0.7879 mL	1.5758 mL	3.9394 mL
	15 mM	0.1051 mL	0.5253 mL	1.0505 mL	2.6263 mL
	20 mM	0.0788 mL	0.3939 mL	0.7879 mL	1.9697 mL
	25 mM	0.0630 mL	0.3152 mL	0.6303 mL	1.5758 mL
	30 mM	0.0525 mL	0.2626 mL	0.5253 mL	1.3131 mL
	40 mM	0.0394 mL	0.1970 mL	0.3939 mL	0.9849 mL
	50 mM	0.0315 mL	0.1576 mL	0.3152 mL	0.7879 mL
	60 mM	0.0263 mL	0.1313 mL	0.2626 mL	0.6566 mL
DMSO	80 mM	0.0197 mL	0.0985 mL	0.1970 mL	0.4924 mL
DMSO	100 mM	0.0158 mL	0.0788 mL	0.1576 mL	0.3939 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Novobiocin sodium Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Novobiocin sodium (Synonyms: Albamycin sodium; Cathomycin sodium)

고객리뷰

Other Forms of Novobiocin sodium:

Novobiocin sodium Related Antibodies

12 Publications Citing Use of MCE Novobiocin sodium

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Antibiotic Isoform Specific Products:

View All DNA/RNA Synthesis Isoform Specific Products:

View All HSP Isoform Specific Products:

Biological Activity

순도&문서

References

고객리뷰

Novobiocin sodium Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

Novobiocin sodium Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report