1. Anti-infection Cell Cycle/DNA Damage Metabolic Enzyme/Protease Apoptosis
  2. Antibiotic DNA/RNA Synthesis HSP Apoptosis Bacterial Orthopoxvirus
  3. Novobiocin

Novobiocin  (Synonyms: Albamycin; Cathomycin)

Cat. No.: HY-B0425 Purity: 97.88%
Handling Instructions Technical Support

Novobiocin (Albamycin) is a potent and orally active antibiotic. Novobiocin also is a DNA gyrase inhibitor and a heat shock protein 90 (Hsp90) antagonist. Novobiocin has the potential for the research of highly beta-lactam-resistant pneumococcal infections. Novobiocin shows anti-orthopoxvirus activity.

For research use only. We do not sell to patients.

CAS No. : 303-81-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
In-stock
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10 mM * 1 mL in DMSO In-stock
Solid
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Customer Review

Based on 12 publication(s) in Google Scholar

Other Forms of Novobiocin:

Top Publications Citing Use of Products

    Novobiocin purchased from MedChemExpress. Usage Cited in: J Med Virol. 2025 Oct;97(10):e70655.  [Abstract]

    Novobiocin also reduced NP expression at 25 μM, with mean fluorescence intensities significantly lower than those of the untreated control.

    Novobiocin purchased from MedChemExpress. Usage Cited in: J Med Virol. 2025 Oct;97(10):e70655.  [Abstract]

    Cytoxicity and inhibition of Novobiocin, EC50 = 25.12 μM.

    Novobiocin purchased from MedChemExpress. Usage Cited in: Nat Methods. 2023 Sep;20(9):1388-1399.  [Abstract]

    Editing efficiencies of the VCAN target using Cas9D10A double nicking in H9 hESCs carrying the K3753R mutation and additional small molecules to inhibit the MMEJ repair proteins Polϴ, PARP or Ligase I/III: Novobiocin.

    Novobiocin purchased from MedChemExpress. Usage Cited in: Blood. 2018 Jul 19;132(3):307-320.  [Abstract]

    K562, KCL22 and HL60 are treated with the indicated (cytotoxic) concentration of Amidopyrine (AX), Novobiocin (NB) and AUY922 for 48h and later protein lysates are subjected to immunoblot analysis.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Novobiocin (Albamycin) is a potent and orally active antibiotic. Novobiocin also is a DNA gyrase inhibitor and a heat shock protein 90 (Hsp90) antagonist. Novobiocin has the potential for the research of highly beta-lactam-resistant pneumococcal infections. Novobiocin shows anti-orthopoxvirus activity[1][2][3][4][6].

    IC50 & Target[1]

    β-lactam

     

    HSP90

     

    Cellular Effect
    Cell Line Type Value Description References
    HCC1937 IC50
    > 80 μM
    Compound: Novobiocin
    Antiproliferative activity against BRCA1-deficient human HCC1937 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay
    Antiproliferative activity against BRCA1-deficient human HCC1937 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay
    [PMID: 38375763]
    HCT-116 IC50
    > 80 μM
    Compound: Novobiocin
    Antiproliferative activity against BRCA2-deficient human HCT-116 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay
    Antiproliferative activity against BRCA2-deficient human HCT-116 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay
    [PMID: 38375763]
    HEK-293T IC50
    > 100 μM
    Compound: NB
    Cytotoxicity against HEK293T cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Cytotoxicity against HEK293T cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    [PMID: 38107168]
    HepG2 IC50
    > 32 μg/mL
    Compound: Novobiocin 1
    Cytotoxicity against human HepG2 cells after 24 hrs by CellTiter-Glo assay
    Cytotoxicity against human HepG2 cells after 24 hrs by CellTiter-Glo assay
    [PMID: 30122228]
    Huh-7 CC50
    1103.18 μg/mL
    Compound: 33
    Cytotoxicity against human HuH7 cells by MTT assay
    Cytotoxicity against human HuH7 cells by MTT assay
    [PMID: 31549836]
    LNCaP IC50
    420 μM
    Compound: NB
    Antiproliferative activity against human LNCAP cells
    Antiproliferative activity against human LNCAP cells
    [PMID: 21129982]
    MCF-10A IC50
    > 80 μM
    Compound: Novobiocin
    Antiproliferative activity against human MCF-10A cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay
    Antiproliferative activity against human MCF-10A cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay
    [PMID: 38375763]
    MCF7 GI50
    260 μM
    Compound: NVB
    Growth inhibition of ER-positive human MCF7 cells after 72 hrs by MTT assay
    Growth inhibition of ER-positive human MCF7 cells after 72 hrs by MTT assay
    [PMID: 24992077]
    MCF7 IC50
    256 μM
    Compound: 31
    Antiproliferative activity against human MCF7 cells
    Antiproliferative activity against human MCF7 cells
    [PMID: 31663736]
    MCF7 IC50
    260 μM
    Compound: NVB
    Antiproliferative activity against human MCF7 cells assessed as inhibition of cell proliferation
    Antiproliferative activity against human MCF7 cells assessed as inhibition of cell proliferation
    [PMID: 24992077]
    MCF7 IC50
    260 μM
    Compound: NVB, novobiocin
    Growth inhibition of MCF7 cells after 72 hrs by MTT assay
    Growth inhibition of MCF7 cells after 72 hrs by MTT assay
    [PMID: 17979263]
    MCF7 IC50
    260 μM
    Compound: Novobiocin
    Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth
    Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth
    [PMID: 34952432]
    MCF7 IC50
    293.3 μM
    Compound: Novobiocin
    Antiproliferative activity against human MCF7 cells measured after 48 hrs by MTT assay
    Antiproliferative activity against human MCF7 cells measured after 48 hrs by MTT assay
    [PMID: 27153346]
    MCF7 IC50
    357 μM
    Compound: 1a, NB
    Cytotoxicity against ER-positive human MCF7 cells expressing low level of HER2 after 3 days by MTT assay
    Cytotoxicity against ER-positive human MCF7 cells expressing low level of HER2 after 3 days by MTT assay
    [PMID: 23859777]
    MCF7 IC50
    481.3 μM
    Compound: novobiocin
    Antiproliferative activity against human MCF7 cells expressing ER
    Antiproliferative activity against human MCF7 cells expressing ER
    [PMID: 20570149]
    MCF7 IC50
    85.1 μM
    Compound: Novobiocin
    Inhibition of BCRP expressed in MCF7 MX cells by Hoechst 33342 staining
    Inhibition of BCRP expressed in MCF7 MX cells by Hoechst 33342 staining
    [PMID: 19932960]
    MDA-MB-436 IC50
    24.3 μM
    Compound: Novobiocin
    Antiproliferative activity against BRCA1-deficient human MDA-MB-436 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay
    Antiproliferative activity against BRCA1-deficient human MDA-MB-436 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay
    [PMID: 38375763]
    MDA-MB-468 IC50
    338.4 μM
    Compound: Novobiocin
    Antiproliferative activity against human MDA-MB-468 cells measured after 48 hrs by MTT assay
    Antiproliferative activity against human MDA-MB-468 cells measured after 48 hrs by MTT assay
    [PMID: 27153346]
    MDCK IC50
    104 μM
    Compound: Novobiocin
    Inhibition of BCRP expressed in MDCK cells by pheophorbide A assay
    Inhibition of BCRP expressed in MDCK cells by pheophorbide A assay
    [PMID: 19932960]
    SH-SY5Y EC50
    48.39 nM
    Compound: novobiocin
    Neuroprotection against beta-amyloid peptide 1-42-induced toxicity in human SH-SY5Y cells assessed as lactate dehydrogenase release
    Neuroprotection against beta-amyloid peptide 1-42-induced toxicity in human SH-SY5Y cells assessed as lactate dehydrogenase release
    [PMID: 19138859]
    SK-BR-3 EC50
    700 μM
    Compound: 1
    Antiproliferative activity against human SKBR3 cells
    Antiproliferative activity against human SKBR3 cells
    [PMID: 29720349]
    SK-BR-3 IC50
    350 μM
    Compound: 1a, NB
    Cytotoxicity against ER-negative human SKBR3 cells expressing high level of HER2 after 3 days by MTT assay
    Cytotoxicity against ER-negative human SKBR3 cells expressing high level of HER2 after 3 days by MTT assay
    [PMID: 23859777]
    SK-BR-3 IC50
    474.7 μM
    Compound: novobiocin
    Antiproliferative activity against human ER deficient SKBR3 cells over expressing HER2
    Antiproliferative activity against human ER deficient SKBR3 cells over expressing HER2
    [PMID: 20570149]
    SK-BR-3 IC50
    700 mM
    Compound: 1
    Antiproliferative activity against human SKBR3 cells
    Antiproliferative activity against human SKBR3 cells
    [PMID: 24953820]
    SK-BR-3 IC50
    700 μM
    Compound: 1
    Antiproliferative activity against human SKBR3 cells after 72 hrs by MTS/PMS assay
    Antiproliferative activity against human SKBR3 cells after 72 hrs by MTS/PMS assay
    [PMID: 19932969]
    SK-BR-3 IC50
    700 μM
    Compound: 1
    Antiproliferative activity against human SKBR3 cells after 72 hrs by MTS assay
    Antiproliferative activity against human SKBR3 cells after 72 hrs by MTS assay
    [PMID: 24900777]
    SK-BR-3 IC50
    700 μM
    Compound: 1
    Antiproliferative activity against human SKBR3 cells
    Antiproliferative activity against human SKBR3 cells
    [PMID: 25699150]
    SK-BR-3 IC50
    700 μM
    Compound: 5
    Antiproliferative activity against human SKBR3 cells after 72 hrs by MTS/PMS assay
    Antiproliferative activity against human SKBR3 cells after 72 hrs by MTS/PMS assay
    [PMID: 25462258]
    SK-BR-3 IC50
    700 μM
    Compound: Novobiocin
    Inhibition of human SKBR3 cell proliferation
    Inhibition of human SKBR3 cell proliferation
    10.1039/C0MD00063A
    SK-BR-3 IC50
    700 μM
    Compound: Novobiocin
    Antiproliferative activity against human SKBR3 cells expressing HER2 after 72 hrs by MTS/PMS assay
    Antiproliferative activity against human SKBR3 cells expressing HER2 after 72 hrs by MTS/PMS assay
    [PMID: 21553822]
    SK-BR-3 IC50
    700 μM
    Compound: Novobiocin
    Antiproliferative activity against human SKBR3 cells
    Antiproliferative activity against human SKBR3 cells
    [PMID: 23234644]
    SK-BR-3 IC50
    700 μM
    Compound: Novobiocin
    Antiproliferative activity against human SKBR3 cells
    Antiproliferative activity against human SKBR3 cells
    [PMID: 23606927]
    SK-BR-3 IC50
    700 μM
    Compound: Novobiocin
    Cytotoxicity against human SKBR3 cells by MTS assay
    Cytotoxicity against human SKBR3 cells by MTS assay
    [PMID: 26745854]
    SK-BR-3 IC50
    700 μM
    Compound: Novobiocin
    Antiproliferative activity against human SKBR3 cells after 72 hrs by MTS assay
    Antiproliferative activity against human SKBR3 cells after 72 hrs by MTS assay
    [PMID: 30048133]
    SK-BR-3 IC50
    ~ 700 μM
    Compound: 1
    Antiproliferative activity against human SK-BR-3 cells
    Antiproliferative activity against human SK-BR-3 cells
    [PMID: 38516588]
    SK-OV-3 IC50
    > 80 μM
    Compound: Novobiocin
    Antiproliferative activity against BRCA-proficient human SK-OV-3 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay
    Antiproliferative activity against BRCA-proficient human SK-OV-3 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay
    [PMID: 38375763]
    SW48 IC50
    > 80 μM
    Compound: Novobiocin
    Antiproliferative activity against BRCA2-deficient human SW48 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay
    Antiproliferative activity against BRCA2-deficient human SW48 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay
    [PMID: 38375763]
    Sf9 IC50
    210 nM
    Compound: 1
    Inhibition of Escherichia coli His6-tagged ParC/ParE expressed in baculovirus-infected Sf9 cells by gel electrophoresis
    Inhibition of Escherichia coli His6-tagged ParC/ParE expressed in baculovirus-infected Sf9 cells by gel electrophoresis
    [PMID: 21235241]
    TERT-RPE1 IC50
    358.4 μM
    Compound: 24; NVB
    Antiproliferative activity against P53 knockout human RPE-1 cells assessed as inhibition of cell growth by CellTiter-Glo assay
    Antiproliferative activity against P53 knockout human RPE-1 cells assessed as inhibition of cell growth by CellTiter-Glo assay
    [PMID: 37134182]
    TERT-RPE1 IC50
    86 μM
    Compound: 24; NVB
    Antiproliferative activity against BRCA1 knockout human RPE-1 cells assessed as inhibition of cell growth by CellTiter-Glo assay
    Antiproliferative activity against BRCA1 knockout human RPE-1 cells assessed as inhibition of cell growth by CellTiter-Glo assay
    [PMID: 37134182]
    TERT-RPE1 IC50
    97.24 μM
    Compound: 24; NVB
    Antiproliferative activity against P53 and BRCA1 knockout human RPE-1 cells assessed as inhibition of cell growth by CellTiter-Glo assay
    Antiproliferative activity against P53 and BRCA1 knockout human RPE-1 cells assessed as inhibition of cell growth by CellTiter-Glo assay
    [PMID: 37134182]
    Vero CC50
    850.5 μg/mL
    Compound: 33
    Cytotoxicity against African green monkey Vero cells by MTT assay
    Cytotoxicity against African green monkey Vero cells by MTT assay
    [PMID: 31549836]
    Vero EC50
    15.21 μg/mL
    Compound: 33
    Inhibition of NS2B-NS3 protease in Zika virus Puerto Rico/PRVABC5 infected in African green monkey Vero cells assessed as antiviral activity measured 48 hrs post infection by RT-PCR method
    Inhibition of NS2B-NS3 protease in Zika virus Puerto Rico/PRVABC5 infected in African green monkey Vero cells assessed as antiviral activity measured 48 hrs post infection by RT-PCR method
    [PMID: 31549836]
    In Vitro

    Novobiocin (1 mM) competitively inhibits ATP binding to gyrase B to interfere with nucleotide binding and interferes with the association of the co-chaperones Hsc70 and p23 with Hsp90[1].
    Novobiocin (200 µM; 24 h) inhibits the rate of repair of both cis-DDP and BCNU induced DNA interstrand cross-links and with a corresponding decrease in the clonogenic survival of the human glioblastoma multiforme cells[2].
    Novobiocin (0.3 mM; 48 hours) induces a caspase-3/7 enzyme–dependent apoptosis assays with an induction of approximately three- to fivefold of apoptotic cells in K562, HL60, Mutz-2[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Novobiocin (25, 50, 100, 200 mg/kg; s.c.; 4 times at 1, 5, 24 and 48 h after infection) shows anti-infection activity in mice infected with amoxicillin-resistant Streptococcus pneumoniae[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 30 g adult female Swiss mice (sepsis induced by the penicillin-susceptible strain (AR33118))[3]
    Dosage: 25, 50, 100, 200 mg/kg
    Administration: S.c.; given at 1, 5, 24 and 48 h after infection
    Result: Showed anti-infection activity in mice infected with amoxicillin-resistant S. pneumoniae.
    Clinical Trial
    Molecular Weight

    612.62

    Formula

    C31H36N2O11

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CC1=C(O2)C(C(O)=C(NC(C3=CC(C/C=C(C)/C)=C(O)C=C3)=O)C2=O)=CC=C1O[C@H]4[C@@H]([C@@H]([C@@H](OC)C(C)(C)O4)OC(N)=O)O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (163.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6323 mL 8.1617 mL 16.3233 mL
    5 mM 0.3265 mL 1.6323 mL 3.2647 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 5 mg/mL (8.16 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 5 mg/mL (8.16 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 97.88%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.6323 mL 8.1617 mL 16.3233 mL 40.8083 mL
    5 mM 0.3265 mL 1.6323 mL 3.2647 mL 8.1617 mL
    10 mM 0.1632 mL 0.8162 mL 1.6323 mL 4.0808 mL
    15 mM 0.1088 mL 0.5441 mL 1.0882 mL 2.7206 mL
    20 mM 0.0816 mL 0.4081 mL 0.8162 mL 2.0404 mL
    25 mM 0.0653 mL 0.3265 mL 0.6529 mL 1.6323 mL
    30 mM 0.0544 mL 0.2721 mL 0.5441 mL 1.3603 mL
    40 mM 0.0408 mL 0.2040 mL 0.4081 mL 1.0202 mL
    50 mM 0.0326 mL 0.1632 mL 0.3265 mL 0.8162 mL
    60 mM 0.0272 mL 0.1360 mL 0.2721 mL 0.6801 mL
    80 mM 0.0204 mL 0.1020 mL 0.2040 mL 0.5101 mL
    100 mM 0.0163 mL 0.0816 mL 0.1632 mL 0.4081 mL
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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Novobiocin
    Cat. No.:
    HY-B0425
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