2. PI3K/Akt/mTOR Metabolic Enzyme/Protease Autophagy Apoptosis NF-κB Immunology/Inflammation Membrane Transporter/Ion Channel Neuronal Signaling
  3. mTOR Akt FXR PI3K Autophagy Ferroptosis Apoptosis Reactive Oxygen Species (ROS) Calcium Channel
  4. Typhaneoside

Typhaneoside is an orally active activator of PI3K/Akt/mTOR and farnesoid X receptor. Typhaneoside promotes the activation of AMPK and Caspase-3, induces apoptosis, ferroptosis, autophagy, ROS accumulation, cell cycle arrest at the G2/M phase, and reduces cancer cell viability. Typhaneoside improves glucose and lipid metabolism, alleviates inflammatory responses, oxidative stress and hepatic lipid accumulation, and exerts hepatoprotective effects. Typhaneoside can be used in research related to heart failure after myocardial infarction, acute myeloid leukemia, non-alcoholic fatty liver disease and neurological disorders.

For research use only. We do not sell to patients.

Typhaneoside

Typhaneoside Chemical Structure

CAS No. : 104472-68-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Typhaneoside:

Typhaneoside Related Antibodies

Top Publications Citing Use of Products

View All mTOR Isoform Specific Products:

View All Isoforms
mTOR mTORC1 mTORC2

View All Akt Isoform Specific Products:

View All Isoforms
Akt Akt1 Akt2 Akt3

View All PI3K Isoform Specific Products:

View All Isoforms
PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

View All Calcium Channel Isoform Specific Products:

View All Isoforms
N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Typhaneoside is an orally active activator of PI3K/Akt/mTOR and farnesoid X receptor. Typhaneoside promotes the activation of AMPK and Caspase-3, induces apoptosis, ferroptosis, autophagy, ROS accumulation, cell cycle arrest at the G2/M phase, and reduces cancer cell viability. Typhaneoside improves glucose and lipid metabolism, alleviates inflammatory responses, oxidative stress and hepatic lipid accumulation, and exerts hepatoprotective effects. Typhaneoside can be used in research related to heart failure after myocardial infarction, acute myeloid leukemia, non-alcoholic fatty liver disease and neurological disorders[1][2][3][4].

Cellular Effect
Cell Line Type Value Description References
N9 IC50
> 100 μM
Compound: 10
Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
[PMID: 28073678]
Raji IC50
592 molar ratio
Compound: 12
Inhibition of TPA-induced EBV-early antigen activation in human Raji cells relative to TPA
Inhibition of TPA-induced EBV-early antigen activation in human Raji cells relative to TPA
[PMID: 17190444]
In Vitro

Typhaneoside (0-50 μM; 12-48 h) significantly reduces the viability of Kas-1, HL60 and NB4 acute myeloid leukemia cells in a time- and dose-dependent manner, while exerts no significant effect on K562 acute myeloid leukemia cells or normal 293T cells[2].
Typhaneoside (20-40 μM; 24 h) induces apoptosis in Kas-1, HL60 and NB4 acute myeloid leukemia cells in a dose-dependent manner, with the highest apoptosis rate induced by treatment at 40 μM[2].
Typhaneoside (20-40 μM; 24 h) dose-dependently reduces the mRNA expression levels of genes associated with mitochondrial dysfunction (NDUFS3, SDHB, UQCRFS1, TFAM, ClpP) in Kas-1, HL60 and NB4 acute myeloid leukemia cells[2].
The effects of typhaneoside (40 μM; 24 h) on inducing reactive oxygen species production and apoptosis in Kas-1, HL60 and NB4 acute myeloid leukemia cells are significantly reversed by pre-treatment with NAC or DFO[2].
Typhaneoside (40 μM; 24 h) upregulates autophagy-related proteins (ATG5, ATG7, Beclin 1, LC3) in Kas-1, HL60, and NB4 acute myeloid leukemia cells, and this effect is reversed by pre-treatment with 5 mM NAC for 2 h[2].
Typhaneoside (12.5-50 μM; 24 h) activates FXR-dependent BSEP promoter activity in HEK293T cells, with significant activation observed at the concentration of 50 μM after 24 h[3].
Typhaneoside (12.5-50 μM; 24 h) alleviates OAPA-induced lipid accumulation, oxidative stress, inflammatory response and glucose metabolism disorder in HepG2 cells by activating the AKT/GSK3β signaling pathway[3].
Typhaneoside (5-50 μM; 10 min) concentration-dependently inhibits 4-aminopyridine (HY-B0604)-induced glutamate release from rat cerebral cortical synaptosomes, with an IC50 of 20 μM[4].
Typhaneoside (20 μM; 10 min)-mediated inhibition of 4-aminopyridine-induced glutamate release from rat cerebral cortex synaptosomes targets Ca2+-dependent vesicular exocytosis, rather than reverse transport via Ca2+-independent glutamate transporters[4].
Typhaneoside (20 μM) inhibits KCl-induced glutamate release from rat cerebral cortex synaptosomes[4].
Typhaneoside (20 μM; 10 min)-mediated inhibition of 4-aminopyridine-induced glutamate release from rat cerebral cortex synaptosomes depends on N-type (Cav2.2) voltage-dependent Ca2+ channels, and does not involve intracellular Ca2+ release or mitochondrial Na+/Ca2+ exchange[4].
The inhibitory effect of Typhaneoside (20 μM; 10 min) on 4-aminopyridine-induced glutamate release from rat cerebral cortex synaptosomes depends on the MAPK/ERK signaling pathway, and does not involve PKA or PKC[4].
Typhaneoside (20 μM; 10 min) reduces the phosphorylation levels of 4-aminopyridine-induced ERK1/2 and its presynaptic target synapsin I in rat cerebral cortex synaptosomes, but exerts no such effect on JNK or p38[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Typhaneoside Related Antibodies

Cell Viability Assay[2]

Cell Line: Kas-1, HL60, NB4, K562, 293T
Concentration: 0, 10, 20, 30, 40 and 50 μM
Incubation Time: 12 h, 24 h, 48 h
Result: Reduced cell viability of Kas-1, HL60, and NB4 cells in a time- and dose-dependent manner.
Showed no significant effect on cell viability of K562 or 293T cells.

Cell Cycle Analysis[2]

Cell Line: Kas-1, HL60, NB4
Concentration: 20, 30 and 40 μM
Incubation Time: 24 h
Result: Dose-dependently arrested Kas-1, HL60, and NB4 cells at the G2/M phase.
Decreased G1/G0 phase cell percentage in a dose-dependent manner for all three cell lines.
Decreased S phase cell percentage in a dose-dependent manner for all three cell lines.

Western Blot Analysis[2]

Cell Line: Kas-1, HL60, NB4
Concentration: 20, 30 and 40 μM
Incubation Time: 24 h
Result: Dose-dependently reduced protein expression of Cyclin B1 and p-Cdc2 in Kas-1, HL60, and NB4 cells.
Dose-dependently enhanced protein expression of p53 and p27 in Kas-1, HL60, and NB4 cells.\nDose-dependently reduced protein expression of anti-apoptotic Bcl-2 in Kas-1, HL60, and NB4 cells.
Dose-dependently increased protein expression of pro-apoptotic Bax and cleaved Caspase-3 in Kas-1, HL60, and NB4 cells.\nDose-dependently increased protein expression of p-AMPK in Kas-1, HL60, and NB4 cells.
Dose-dependently decreased protein expression of p-mTOR in Kas-1, HL60, and NB4 cells.\nDose-dependently increased protein expression of ATG5, ATG7, Beclin 1, and LC3 in Kas-1, HL60, and NB4 cells.

Western Blot Analysis[2]

Cell Line: Kas-1, HL60, NB4
Concentration: 40 μM
Incubation Time: 24 h (with 2 h pre-treatment of 5 mM NAC)
Result: Induced upregulation of ATG5, ATG7, Beclin 1, and LC3 protein expression that was reversed by pretreatment with 5 mM NAC in Kas-1, HL60, and NB4 cells.
In Vivo

Typhaneoside (10-40 mg/kg; once daily; 4 weeks) improves cardiac function, normalizes hemodynamic parameters, reduces pro-inflammatory and cardiac remodeling biomarkers, and inhibits excessive autophagy via activation of the PI3K/Akt/mTOR pathway in a dose-dependent manner in rats with heart failure post-myocardial infarction[1].
Typhaneoside (10-30 mg/kg; i.p.; daily; 30 days) dose-dependently suppresses AML tumor growth in BALB/c nude mice, with the 30 mg/kg dose achieving the greatest tumor volume reduction and the highest 30-day survival rate of ~50%, while showing no overt tissue toxicity[2].
Typhaneoside (30 mg/kg; i.p.; daily; 30 days) causes no overt tissue toxicity in healthy BALB/c nude mice[2].
Typhaneoside (15-60 mg/kg/day; p.o.; daily; 8 weeks) dose-dependently alleviates HFD-induced NAFLD in male C57BL/6 mice by activating FXR signaling, reducing body and adipose tissue weight, improving lipid and glucose homeostasis, mitigating liver injury, oxidative stress and inflammation, and enhancing BAT thermogenesis and energy expenditure[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c nude (4-week-old male; AML model via subcutaneous injection of 1×107 HL60 cells, tumor grown to 50 mm3 before treatment)[2]
Dosage: 10 mg/kg; 20 mg/kg; 30 mg/kg
Administration: i.p.; daily; 30 days
Result: Significantly reduced mean tumor volume to ~1000 mm3 (10 mg/kg), ~800 mm3 (20 mg/kg), and ~500 mm3 (30 mg/kg) at day 30 compared to controls.
Improved 30-day survival rate to ~20% (10 mg/kg), ~30% (20 mg/kg), and ~50% (30 mg/kg) in a dose-dependent manner.
Significantly reduced white blood cell counts in a dose-dependent manner compared to controls.
Animal Model: C57BL/6 (male, 8-week-old, 20-22 g, SPF, HFD-induced NAFLD)[3]
Dosage: 15 mg/kg/day; 30 mg/kg/day; 60 mg/kg/day
Administration: p.o.; daily; 8 weeks
Result: Reduced HFD-induced body weight gain in a dose-dependent manner, with a significant decrease in area under the curve for body weight across all doses.
Dose-dependently reduced liver weight,
Dose-dependently reversed HFD-induced serum and hepatic lipid abnormalities: decreased serum triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and free fatty acid (FFA) levels; increased serum high-density lipoprotein cholesterol (HDL-C) levels; decreased hepatic TG levels.
Dose-dependently reversed HFD-induced liver injury, oxidative stress, and inflammation: decreased serum and hepatic AST, ALT, and MDA levels; increased serum and hepatic SOD levels; suppressed HFD-induced upregulation of proinflammatory mRNA (IL-6, IL-1β, NF-κB, TNF-α) in liver and WAT.
Dose-dependently upregulated mRNA expression of FXR, SHP, BSEP, and TGR5 in liver, and increased hepatic FXR protein expression detected by immunofluorescence.
Molecular Weight

770.69

Formula

C34H42O20
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C(O[C@H](O[C@H](CO[C@H](O[C@@H](C)[C@H](O)[C@H]2O)[C@@H]2O)[C@@H](O)[C@@H]3O)[C@@H]3O[C@@](O[C@@H](C)[C@H](O)[C@H]4O)([H])[C@@H]4O)=C(C5=CC(OC)=C(O)C=C5)OC6=CC(O)=CC(O)=C16
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (324.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2975 mL 6.4877 mL 12.9754 mL
5 mM 0.2595 mL 1.2975 mL 2.5951 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (2.70 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (2.70 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.74%

SDS (393 KB)

COA (262 KB) HNMR (171 KB) LCMS (84 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2975 mL 6.4877 mL 12.9754 mL 32.4385 mL
5 mM 0.2595 mL 1.2975 mL 2.5951 mL 6.4877 mL
10 mM 0.1298 mL 0.6488 mL 1.2975 mL 3.2438 mL
15 mM 0.0865 mL 0.4325 mL 0.8650 mL 2.1626 mL
20 mM 0.0649 mL 0.3244 mL 0.6488 mL 1.6219 mL
25 mM 0.0519 mL 0.2595 mL 0.5190 mL 1.2975 mL
30 mM 0.0433 mL 0.2163 mL 0.4325 mL 1.0813 mL
40 mM 0.0324 mL 0.1622 mL 0.3244 mL 0.8110 mL
50 mM 0.0260 mL 0.1298 mL 0.2595 mL 0.6488 mL
60 mM 0.0216 mL 0.1081 mL 0.2163 mL 0.5406 mL
80 mM 0.0162 mL 0.0811 mL 0.1622 mL 0.4055 mL
100 mM 0.0130 mL 0.0649 mL 0.1298 mL 0.3244 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Typhaneoside Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Typhaneoside104472-68-6mTORAktFXRPI3KAutophagyFerroptosisApoptosisReactive Oxygen Species (ROS)Calcium ChannelMammalian target of RapamycinPKBProtein kinase BNR1H4Phosphoinositide 3-kinaseCa2+ channelsCa channelsPI3K/Akt/mTOR autophagy transduction pathwayacute myeloid leukemiafarnesoid X receptortumor necrosis factor alphamatrix metalloproteinase 2interleukin-6matrix metalloproteinase 9cardiomyocyteInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Typhaneoside
Cat. No.:
HY-N0712
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB) Italian - IT (393 KB) Korean - KR (393 KB) Portuguese - PT (393 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.