ダウノルビシン塩酸塩 (Synonyms: Daunorubicin (hydrochloride); Rubidomycin (hydrochloride); Daunomycin (hydrochloride); RP 13057 (hydrochloride))

Name: Daunorubicin hydrochloride
Brand: MedChemExpress
SKU: HY-13062
Rating: 5.0 (37 reviews)

製品番号: HY-13062 純度: 99.67%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

Daunorubicin (Daunomycin) hydrochloride is a topoisomerase II inhibitor with potent anti-tumor activity. Daunorubicin hydrochloride inhibits DNA and RNA synthesis. Daunorubicin hydrochloride is a cytotoxin that inhibits cancer cell viability and induces apoptosis and necrosis. Daunorubicin hydrochloride is also an anthracycline antibiotic. Daunorubicin hydrochloride can be used in the research of infection and variety of cancers, including leukemia, non-Hodgkin lymphomas, Ewing's sarcoma, Wilms' tumor.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 23541-50-6

容量	価格（税別）	在庫状況	数量

>無料サンプル (0.1 - 0.2 mg)		今すぐ申し込む
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 51	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 51	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$41	在庫あり	Estimated Time of Arrival: December 31
10 mg	$65	在庫あり	Estimated Time of Arrival: December 31
25 mg	$105	在庫あり	Estimated Time of Arrival: December 31
50 mg	$150	在庫あり	Estimated Time of Arrival: December 31
100 mg	$250	在庫あり	Estimated Time of Arrival: December 31
200 mg	$380	在庫あり	Estimated Time of Arrival: December 31
500 mg	$594	在庫あり	Estimated Time of Arrival: December 31
1 g		お問い合わせ
5 g		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 37 publication(s) in Google Scholar

Other Forms of Daunorubicin hydrochloride:

Daunorubicin 在庫あり
Daunorubicin citrate お問い合わせ
Daunorubicin hydrochloride (Standard) お問い合わせ

顧客検証

Cell Proliferation/Viability Assay

Apoptosis Analysis

Cell Imaging/Staining

: Daunorubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Interdiscip Med. 2025 Sep 1;3(5):e70053.; Cell viability analysis of OCI-AML2 cells expressing the indicated sgRNAs following treatment with increasing concentrations of Daunorubicin hydrochloride (3 days), assessed using the CCK-8 assay.

: Daunorubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Interdiscip Med. 2025 Sep 1;3(5):e70053.; OCI-AML2 cells expressing indicated sgRNAs were treated with DMSO or Daunorubicin hydrochloride (10 nM), and viable cells were counted every 3–4 days.

: Daunorubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Jan;20(1):51-64. [Abstract]; Comparison of drug (Epirubicin hydrochloride, EPI; Daunorubicin hydrochloride, DNR; Vinorelbine ditartrate, VNR; Oxaliplatin, OXA; Vincristine, VCR; Artemisinin, ART; Colchicine, COL) cytotoxicity to TC1 cells at the indicated doses and time points measured with CCK-8 assays. R.U. (Relative unit) was calculated from the average O.D. values in each condition as the indicator of cell viability (n = 3).

: Daunorubicin hydrochloride purchased from MedChemExpress. Usage Cited in: J Transl Med. 2022 Jul 6;20(1):304. [Abstract]; IC₅₀ curves of Daunorubicin hydrochloride (1.25-1280 nM; 48 h) in U937 and Molm-13 cells transfected with lentivirus targeting SEMA4D or control. Transduced AML cells were treated with daunorubicin for 48 h and then measured by CCK-8 analysis.

: Daunorubicin hydrochloride purchased from MedChemExpress. Usage Cited in: J Transl Med. 2022 Jul 6;20(1):304. [Abstract]; Cell apoptosis rate of U937 and Molm-13 cells transfected with lentivirus targeting SEMA4D or control after Daunorubicin hydrochloride (10-80 nM) treatment was detected by flow cytometry using Annexin V-APC /PI staining.

: Daunorubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Pharmaceutics. 2021 May 5;13(5):661.; Primary AML cells were treated with Daunorubicin hydrochloride (DNR, 8-32 μM) and Erastin (5 μM) for 48 h, and cell viability was assessed using the CCK-8 assay.

: Daunorubicin hydrochloride purchased from MedChemExpress. Usage Cited in: Pharmaceutics. 2021 May 5;13(5):661.; HL-60 cells were pretreated with CCL20 for 30 min, followed by Daunorubicin hydrochloride (DNR, 1.6, 3.2 μM) treatment for 48 h, and lipid peroxidation level were measured.

Topoisomerase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Topoisomerase Topo I Topo II DNA gyrase

DNA/RNA Synthesis アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

ADC Payload アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Auristatin Calicheamicins Camptothecins Daunorubicins/Doxorubicins Duocarmycins Maytansinoids Pyrrolobenzodiazepines Traditional Cytotoxic Agents

Antibiotic アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

IC₅₀ & Target^[1]^[2]

Topoisomerase II

Daunorubicins/Doxorubicins

Cellular Effect

Cell Line	Type	Value	Description	References
CAPAN-1	IC₅₀	3.1 3 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against Capen-1 cell line (pancreatic carcinoma) after incubation for 72 hour Cytotoxicity of compound was tested against Capen-1 cell line (pancreatic carcinoma) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
A204	IC₅₀	3 3 Compound: Daunomycin	Antiproliferative activity of compound was tested against rhabdomyosarcoma (A-204) human tumor cells Antiproliferative activity of compound was tested against rhabdomyosarcoma (A-204) human tumor cells	10.1016/0960-894X(95)00523-3
A204	IC₅₀	3 3 Compound: Daunomycin	Antiproliferative activity of compound was tested against rhabdomyosarcoma (A-204) human tumor cells Antiproliferative activity of compound was tested against rhabdomyosarcoma (A-204) human tumor cells	10.1016/0960-894X(95)00523-3
CAPAN-1	IC₅₀	3.9 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against Capan-1 cell line (pancreatic carcinoma) after incubation for 24 hour Cytotoxicity of compound was tested against Capan-1 cell line (pancreatic carcinoma) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
A498	IC₅₀	3 3 Compound: Daunomycin	Antiproliferative activity of compound was tested against renal cancer (A-498) human tumor cells Antiproliferative activity of compound was tested against renal cancer (A-498) human tumor cells	10.1016/0960-894X(95)00523-3
A498	IC₅₀	3 3 Compound: Daunomycin	Antiproliferative activity of compound was tested against renal cancer (A-498) human tumor cells Antiproliferative activity of compound was tested against renal cancer (A-498) human tumor cells	10.1016/0960-894X(95)00523-3
CHO	IC₅₀	2.94 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against CHO cell line (Chinese hamster ovary) after incubation for 72 hour Cytotoxicity of compound was tested against CHO cell line (Chinese hamster ovary) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
CAPAN-1	IC₅₀	3.9 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against Capan-1 cell line (pancreatic carcinoma) after incubation for 24 hour Cytotoxicity of compound was tested against Capan-1 cell line (pancreatic carcinoma) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
CHO	IC₅₀	2.94 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against CHO cell line (Chinese hamster ovary) after incubation for 72 hour Cytotoxicity of compound was tested against CHO cell line (Chinese hamster ovary) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
CAPAN-1	IC₅₀	3.1 x 10^-6 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against Capen-1 cell line (pancreatic carcinoma) after incubation for 72 hour Cytotoxicity of compound was tested against Capen-1 cell line (pancreatic carcinoma) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
CHO	IC₅₀	3.9 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against CHO cell line (Chinese hamster ovary) after incubation for 24 hour Cytotoxicity of compound was tested against CHO cell line (Chinese hamster ovary) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
P388	IC₅₀	3 1 Compound: Daunorubicin HCl	Growth Inhibition of P388/0 cell lines. Growth Inhibition of P388/0 cell lines.	[PMID: 10669575]
CHO	IC₅₀	2.94 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against CHO cell line (Chinese hamster ovary) after incubation for 72 hour Cytotoxicity of compound was tested against CHO cell line (Chinese hamster ovary) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
HCT-116	IC₅₀	0.28 6 Compound: Daunorubicin HCl	Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay	[PMID: 12141872]
EVSA-T	IC₅₀	2 3 Compound: Daunomycin	Antiproliferative activity of compound was tested against mammary carcinoma (EVSAT) human tumor cells Antiproliferative activity of compound was tested against mammary carcinoma (EVSAT) human tumor cells	10.1016/0960-894X(95)00523-3
HCT-116	IC₅₀	0.28 6 Compound: Daunorubicin HCl	Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay	[PMID: 12141872]
HeLa	IC₅₀	0.32 3 Compound: Daunorubicin	Cytotoxicity against human HeLa cells after 48 hrs by MTT assay Cytotoxicity against human HeLa cells after 48 hrs by MTT assay	[PMID: 17190447]
CHO	IC₅₀	3.9 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against CHO cell line (Chinese hamster ovary) after incubation for 24 hour Cytotoxicity of compound was tested against CHO cell line (Chinese hamster ovary) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
HeLa	IC₅₀	0.32 3 Compound: Daunorubicin	Cytotoxicity against human HeLa cells after 48 hrs by MTT assay Cytotoxicity against human HeLa cells after 48 hrs by MTT assay	[PMID: 17190447]
HL-60	IC₅₀	< 9.8 x 10^-8 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against HL-60 cell line (promyelocytic leukemia) after incubation for 24 hour Cytotoxicity of compound was tested against HL-60 cell line (promyelocytic leukemia) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
HL-60	IC₅₀	9.8 x 10^-8 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against HL-60 cell line (promyelocytic leukemia) after incubation for 72 hour Cytotoxicity of compound was tested against HL-60 cell line (promyelocytic leukemia) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
HRT-18 cell line	IC₅₀	0.11 6 Compound: Daunorubicin HCl	Cytotoxicity against human HRT18 cells after 48 hrs by MTT assay Cytotoxicity against human HRT18 cells after 48 hrs by MTT assay	[PMID: 12141872]
EVSA-T	IC₅₀	2 3 Compound: Daunomycin	Antiproliferative activity of compound was tested against mammary carcinoma (EVSAT) human tumor cells Antiproliferative activity of compound was tested against mammary carcinoma (EVSAT) human tumor cells	10.1016/0960-894X(95)00523-3
HMEC	IC₅₀	< 9.8 x 10^-8 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against HMEC cell line (human mamm epithelial cells) after incubation for 72 hour Cytotoxicity of compound was tested against HMEC cell line (human mamm epithelial cells) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
HT-29	IC₅₀	0.072 6 Compound: Daunorubicin HCl	Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay	[PMID: 12141872]
HCT-116	IC₅₀	0.28 6 Compound: Daunorubicin HCl	Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay	[PMID: 12141872]
HL-60	IC₅₀	< 9.8 x 10^-8 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against HL-60 cell line (promyelocytic leukemia) after incubation for 24 hour Cytotoxicity of compound was tested against HL-60 cell line (promyelocytic leukemia) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
HMEC	IC₅₀	3.9 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against HMEC cell line (human mamm epithelial cells) after incubation for 24 hour Cytotoxicity of compound was tested against HMEC cell line (human mamm epithelial cells) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
HT-29	IC₅₀	3.57 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against HT-29 cell line (colon carcinoma) after incubation for 72 hour Cytotoxicity of compound was tested against HT-29 cell line (colon carcinoma) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
HRT-18 cell line	IC₅₀	0.11 6 Compound: Daunorubicin HCl	Cytotoxicity against human HRT18 cells after 48 hrs by MTT assay Cytotoxicity against human HRT18 cells after 48 hrs by MTT assay	[PMID: 12141872]
IGR-37 cell line	IC₅₀	11 3 Compound: Daunomycin	Antiproliferative activity of compound was tested against melanoma (IgR-37) human tumor cells Antiproliferative activity of compound was tested against melanoma (IgR-37) human tumor cells	10.1016/0960-894X(95)00523-3
HL-60	IC₅₀	9.8 x 10^-8 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against HL-60 cell line (promyelocytic leukemia) after incubation for 72 hour Cytotoxicity of compound was tested against HL-60 cell line (promyelocytic leukemia) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
HT-29	IC₅₀	0.072 6 Compound: Daunorubicin HCl	Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay	[PMID: 12141872]
IGROV-1	IC₅₀	18 3 Compound: Daunomycin	Antiproliferative activity of compound was tested against ovarian carcinoma (IGROV) human tumor cells Antiproliferative activity of compound was tested against ovarian carcinoma (IGROV) human tumor cells	10.1016/0960-894X(95)00523-3
HMEC	IC₅₀	< 9.8 x 10^-8 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against HMEC cell line (human mamm epithelial cells) after incubation for 72 hour Cytotoxicity of compound was tested against HMEC cell line (human mamm epithelial cells) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
HT-29	IC₅₀	3.57 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against HT-29 cell line (colon carcinoma) after incubation for 72 hour Cytotoxicity of compound was tested against HT-29 cell line (colon carcinoma) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
K562	IC₅₀	>5 3 Compound: DNR	Cytotoxicity against doxorubicin-resistant K562 cell line by MTS assay Cytotoxicity against doxorubicin-resistant K562 cell line by MTS assay	[PMID: 16509594]
HMEC	IC₅₀	3.9 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against HMEC cell line (human mamm epithelial cells) after incubation for 24 hour Cytotoxicity of compound was tested against HMEC cell line (human mamm epithelial cells) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
HT-29	IC₅₀	7.8 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against HT-29 cell line (colon carcinoma) after incubation for 24 hour Cytotoxicity of compound was tested against HT-29 cell line (colon carcinoma) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
K562	IC₅₀	0.015 3 Compound: DNR	Cytotoxicity against drug-sensitive K562 cell line by MTS assay Cytotoxicity against drug-sensitive K562 cell line by MTS assay	[PMID: 16509594]
HT-29	IC₅₀	0.072 6 Compound: Daunorubicin HCl	Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay	[PMID: 12141872]
K562	IC₅₀	> 5 3 Compound: DNR	Cytotoxicity against doxorubicin-resistant K562 cell line by MTS assay Cytotoxicity against doxorubicin-resistant K562 cell line by MTS assay	[PMID: 16509594]
K562	IC₅₀	156.6 3 Compound: DNR hydrochloride	Cytotoxicity against K562 leukemia cell line done for 72 hr at 37 degree C with compound in MTS assay Cytotoxicity against K562 leukemia cell line done for 72 hr at 37 degree C with compound in MTS assay	[PMID: 16078845]
HT-29	IC₅₀	3.57 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against HT-29 cell line (colon carcinoma) after incubation for 72 hour Cytotoxicity of compound was tested against HT-29 cell line (colon carcinoma) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
K562	IC₅₀	0.015 3 Compound: DNR	Cytotoxicity against drug-sensitive K562 cell line by MTS assay Cytotoxicity against drug-sensitive K562 cell line by MTS assay	[PMID: 16509594]
HT-29	IC₅₀	7.8 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against HT-29 cell line (colon carcinoma) after incubation for 24 hour Cytotoxicity of compound was tested against HT-29 cell line (colon carcinoma) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
L1210	ED₅₀	1 3 Compound: 2	Dose required to inhibit the DNA synthesis in leukemia L1210 cells Dose required to inhibit the DNA synthesis in leukemia L1210 cells	[PMID: 7252976]
K562	IC₅₀	156.6 3 Compound: DNR hydrochloride	Cytotoxicity against K562 leukemia cell line done for 72 hr at 37 degree C with compound in MTS assay Cytotoxicity against K562 leukemia cell line done for 72 hr at 37 degree C with compound in MTS assay	[PMID: 16078845]
L1210	ED₅₀	0.3 3 Compound: 2	Dose required to inhibit the RNA synthesis in leukemia L1210 cells Dose required to inhibit the RNA synthesis in leukemia L1210 cells	[PMID: 7252976]
HeLa	IC₅₀	0.32 3 Compound: Daunorubicin	Cytotoxicity against human HeLa cells after 48 hrs by MTT assay Cytotoxicity against human HeLa cells after 48 hrs by MTT assay	[PMID: 17190447]
LNCaP	IC₅₀	0.011 6 Compound: Daunorubicin HCl	Cytotoxicity against human LNCAP cells expressing androgen receptor after 48 hrs by MTT assay Cytotoxicity against human LNCAP cells expressing androgen receptor after 48 hrs by MTT assay	[PMID: 12141872]
L1210	ED₅₀	0.66 3 Compound: 1 (Daunorubicin hydrochloride)	Inhibition of [3H]thymidine incorporation into proliferating L1210 cells. Inhibition of [3H]thymidine incorporation into proliferating L1210 cells.	[PMID: 6716401]
MCF7	IC₅₀	0.12 6 Compound: Daunorubicin HCl	Cytotoxicity against human MCF7 cells expressing estrogen receptor after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells expressing estrogen receptor after 48 hrs by MTT assay	[PMID: 12141872]
L1210	ED₅₀	0.33 3 Compound: 1 (Daunorubicin hydrochloride)	Inhibition of [3H]uridine incorporation into proliferating L1210 cells. Inhibition of [3H]uridine incorporation into proliferating L1210 cells.	[PMID: 6716401]
IGROV-1	IC₅₀	18 3 Compound: Daunomycin	Antiproliferative activity of compound was tested against ovarian carcinoma (IGROV) human tumor cells Antiproliferative activity of compound was tested against ovarian carcinoma (IGROV) human tumor cells	10.1016/0960-894X(95)00523-3
L1210	ED₅₀	1 3 Compound: 2	Inhibition of DNA synthesis in mouse L1210 cells Inhibition of DNA synthesis in mouse L1210 cells	[PMID: 423181]
MCF7	IC₅₀	0.22 3 Compound: Daunorubicin	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 17190447]
K562	IC₅₀	0.015 3 Compound: DNR	Cytotoxicity against drug-sensitive K562 cell line by MTS assay Cytotoxicity against drug-sensitive K562 cell line by MTS assay	[PMID: 16509594]
MCF7	IC₅₀	1.35 x 10^-5 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against MCF-7 cell line (breast carcinoma) after incubation for 72 hour Cytotoxicity of compound was tested against MCF-7 cell line (breast carcinoma) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
L1210	ED₅₀	0.4 3 Compound: 1, daunorubicin	Inhibition of DNA synthesis in mouse L1210 cells assessed as inhibition of [3H]thymidine incorporation Inhibition of DNA synthesis in mouse L1210 cells assessed as inhibition of [3H]thymidine incorporation	[PMID: 490537]
K562	IC₅₀	156.6 3 Compound: DNR hydrochloride	Cytotoxicity against K562 leukemia cell line done for 72 hr at 37 degree C with compound in MTS assay Cytotoxicity against K562 leukemia cell line done for 72 hr at 37 degree C with compound in MTS assay	[PMID: 16078845]
MCF7	IC₅₀	7.8 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against MCF-7 cell line (breast carcinoma) after incubation for 24 hour Cytotoxicity of compound was tested against MCF-7 cell line (breast carcinoma) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
L1210	ED₅₀	0.3 3 Compound: 2	Inhibition of RNA synthesis in mouse L1210 cells Inhibition of RNA synthesis in mouse L1210 cells	[PMID: 423181]
K562	IC₅₀	> 5 3 Compound: DNR	Cytotoxicity against doxorubicin-resistant K562 cell line by MTS assay Cytotoxicity against doxorubicin-resistant K562 cell line by MTS assay	[PMID: 16509594]
L1210	ED₅₀	0.3 3 Compound: 1, daunorubicin	Inhibition of RNA synthesis in mouse L1210 cells assessed as inhibition of [3H]uridine incorporation Inhibition of RNA synthesis in mouse L1210 cells assessed as inhibition of [3H]uridine incorporation	[PMID: 490537]
L1210	ED₅₀	0.3 3 Compound: 1, daunorubicin	Inhibition of RNA synthesis in mouse L1210 cells assessed as inhibition of [3H]uridine incorporation Inhibition of RNA synthesis in mouse L1210 cells assessed as inhibition of [3H]uridine incorporation	[PMID: 490537]
MDA-MB-231	IC₅₀	0.25 3 Compound: Daunorubicin	Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay	[PMID: 17190447]
MOLT-4	IC₅₀	1 1 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against Molt-4 cell line (T-cell leukemia) after incubation for 24 hour Cytotoxicity of compound was tested against Molt-4 cell line (T-cell leukemia) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
L1210 (1565)	IC₅₀	0.147 77 Compound: Daunomycin	Cytotoxic activity against adriamycin (ADR) resistant L-1210 cell line Cytotoxic activity against adriamycin (ADR) resistant L-1210 cell line	10.1016/0960-894X(95)00067-4
L1210	ED₅₀	0.3 3 Compound: 2	Inhibition of RNA synthesis in mouse L1210 cells Inhibition of RNA synthesis in mouse L1210 cells	[PMID: 423181]
MOLT-4	IC₅₀	8.55 x 10^-13 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against Molt-4 cell line (T-cell leukemia) after incubation for 72 hour Cytotoxicity of compound was tested against Molt-4 cell line (T-cell leukemia) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
L1210 (1565)	IC₅₀	0.016 77 Compound: Daunomycin	Cytotoxic activity against L-1210 parental cell line Cytotoxic activity against L-1210 parental cell line	10.1016/0960-894X(95)00067-4
L1210	ED₅₀	0.3 3 Compound: 2	Dose required to inhibit the RNA synthesis in leukemia L1210 cells Dose required to inhibit the RNA synthesis in leukemia L1210 cells	[PMID: 7252976]
NHDF	IC₅₀	1.9 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against NHDF cell line (norm human dermal fibroblast) after incubation for 24 hour Cytotoxicity of compound was tested against NHDF cell line (norm human dermal fibroblast) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
L1210	ED₅₀	0.33 3 Compound: 1 (Daunorubicin hydrochloride)	Inhibition of [3H]uridine incorporation into proliferating L1210 cells. Inhibition of [3H]uridine incorporation into proliferating L1210 cells.	[PMID: 6716401]
LNCaP	IC₅₀	0.011 6 Compound: Daunorubicin HCl	Cytotoxicity against human LNCAP cells expressing androgen receptor after 48 hrs by MTT assay Cytotoxicity against human LNCAP cells expressing androgen receptor after 48 hrs by MTT assay	[PMID: 12141872]
NHDF	IC₅₀	4.04 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against NHDF cell line (norm human dermal fibroblast) after incubation for 72 hour Cytotoxicity of compound was tested against NHDF cell line (norm human dermal fibroblast) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
L1210	ED₅₀	0.4 3 Compound: 1, daunorubicin	Inhibition of DNA synthesis in mouse L1210 cells assessed as inhibition of [3H]thymidine incorporation Inhibition of DNA synthesis in mouse L1210 cells assessed as inhibition of [3H]thymidine incorporation	[PMID: 490537]
M19-MEL	IC₅₀	2 3 Compound: Daunomycin	Antiproliferative activity of compound was tested against melanoma (M-19) human tumor cells Antiproliferative activity of compound was tested against melanoma (M-19) human tumor cells	10.1016/0960-894X(95)00523-3
OVCAR-3	IC₅₀	2.87 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against Ovcar-3 cell line (ovarian carcinoma) after incubation for 72 hour Cytotoxicity of compound was tested against Ovcar-3 cell line (ovarian carcinoma) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
MCF7	IC₅₀	2 3 Compound: Daunomycin	Antiproliferative activity of compound was tested against mammary carcinoma (MCF-7) human tumor cells Antiproliferative activity of compound was tested against mammary carcinoma (MCF-7) human tumor cells	10.1016/0960-894X(95)00523-3
L1210	ED₅₀	0.66 3 Compound: 1 (Daunorubicin hydrochloride)	Inhibition of [3H]thymidine incorporation into proliferating L1210 cells. Inhibition of [3H]thymidine incorporation into proliferating L1210 cells.	[PMID: 6716401]
OVCAR-3	IC₅₀	3.9 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against Ovcar-3 cell line (ovarian carcinoma) after incubation for 24 hour Cytotoxicity of compound was tested against Ovcar-3 cell line (ovarian carcinoma) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
MCF7	IC₅₀	0.22 3 Compound: Daunorubicin	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 17190447]
L1210	ED₅₀	1 3 Compound: 2	Inhibition of DNA synthesis in mouse L1210 cells Inhibition of DNA synthesis in mouse L1210 cells	[PMID: 423181]
P388	IC₅₀	< 9.8 x 10^-8 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against P-388 cell line (mouse leukemia) after incubation for 72 hour Cytotoxicity of compound was tested against P-388 cell line (mouse leukemia) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
MCF7	IC₅₀	0.12 6 Compound: Daunorubicin HCl	Cytotoxicity against human MCF7 cells expressing estrogen receptor after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells expressing estrogen receptor after 48 hrs by MTT assay	[PMID: 12141872]
L1210	ED₅₀	1 3 Compound: 2	Dose required to inhibit the DNA synthesis in leukemia L1210 cells Dose required to inhibit the DNA synthesis in leukemia L1210 cells	[PMID: 7252976]
P388	IC₅₀	3.9 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against P-388 cell line (mouse leukemia) after incubation for 24 hour Cytotoxicity of compound was tested against P-388 cell line (mouse leukemia) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
MCF7	IC₅₀	1.35 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against MCF-7 cell line (breast carcinoma) after incubation for 72 hour Cytotoxicity of compound was tested against MCF-7 cell line (breast carcinoma) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
L1210 (1565)	IC₅₀	0.016 3 Compound: Daunomycin	Cytotoxic activity against L-1210 parental cell line Cytotoxic activity against L-1210 parental cell line	10.1016/0960-894X(95)00067-4
PC-3	IC₅₀	0.23 6 Compound: Daunorubicin HCl	Cytotoxicity against human PC3 cells after 48 hrs by MTT assay Cytotoxicity against human PC3 cells after 48 hrs by MTT assay	[PMID: 12141872]
MDA-MB-231	IC₅₀	0.25 3 Compound: Daunorubicin	Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay	[PMID: 17190447]
L1210 (1565)	IC₅₀	0.147 3 Compound: Daunomycin	Cytotoxic activity against adriamycin (ADR) resistant L-1210 cell line Cytotoxic activity against adriamycin (ADR) resistant L-1210 cell line	10.1016/0960-894X(95)00067-4
MOLT-4	IC₅₀	8.55 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against Molt-4 cell line (T-cell leukemia) after incubation for 72 hour Cytotoxicity of compound was tested against Molt-4 cell line (T-cell leukemia) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
PC-3	IC₅₀	0.44 3 Compound: Daunorubicin	Cytotoxicity against human PC-3 cells after 48 hrs by MTT assay Cytotoxicity against human PC-3 cells after 48 hrs by MTT assay	[PMID: 17190447]
SK-MEL-28	IC₅₀	1.41 3 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against SK-Mel-28 cell line (melanoma) after incubation for 72 hour Cytotoxicity of compound was tested against SK-Mel-28 cell line (melanoma) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
NHDF	IC₅₀	4.04 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against NHDF cell line (norm human dermal fibroblast) after incubation for 72 hour Cytotoxicity of compound was tested against NHDF cell line (norm human dermal fibroblast) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
LNCaP	IC₅₀	0.011 6 Compound: Daunorubicin HCl	Cytotoxicity against human LNCAP cells expressing androgen receptor after 48 hrs by MTT assay Cytotoxicity against human LNCAP cells expressing androgen receptor after 48 hrs by MTT assay	[PMID: 12141872]
SK-MEL-28	IC₅₀	1.6 3 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against SK-Mel-28 cell line (melanoma) after incubation for 24 hour Cytotoxicity of compound was tested against SK-Mel-28 cell line (melanoma) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
OVCAR-3	IC₅₀	2.87 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against Ovcar-3 cell line (ovarian carcinoma) after incubation for 72 hour Cytotoxicity of compound was tested against Ovcar-3 cell line (ovarian carcinoma) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
SW-620	IC₅₀	8.6 3 Compound: DNR hydrochloride	Cytotoxicity against SW620 colon cancer cell line done for 72 h at 37 degree C with compound in MTS assay Cytotoxicity against SW620 colon cancer cell line done for 72 h at 37 degree C with compound in MTS assay	[PMID: 16078845]
PC-3	IC₅₀	0.23 6 Compound: Daunorubicin HCl	Cytotoxicity against human PC3 cells after 48 hrs by MTT assay Cytotoxicity against human PC3 cells after 48 hrs by MTT assay	[PMID: 12141872]
M19-MEL	IC₅₀	2 3 Compound: Daunomycin	Antiproliferative activity of compound was tested against melanoma (M-19) human tumor cells Antiproliferative activity of compound was tested against melanoma (M-19) human tumor cells	10.1016/0960-894X(95)00523-3
UCLA P-3	IC₅₀	7.3 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against UCLA P-3 cell line (lung carcinoma) after incubation for 24 hour Cytotoxicity of compound was tested against UCLA P-3 cell line (lung carcinoma) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
PC-3	IC₅₀	0.44 3 Compound: Daunorubicin	Cytotoxicity against human PC-3 cells after 48 hrs by MTT assay Cytotoxicity against human PC-3 cells after 48 hrs by MTT assay	[PMID: 17190447]
SK-MEL-28	IC₅₀	1.41 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against SK-Mel-28 cell line (melanoma) after incubation for 72 hour Cytotoxicity of compound was tested against SK-Mel-28 cell line (melanoma) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
MCF7	IC₅₀	0.12 6 Compound: Daunorubicin HCl	Cytotoxicity against human MCF7 cells expressing estrogen receptor after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells expressing estrogen receptor after 48 hrs by MTT assay	[PMID: 12141872]
SW-620	IC₅₀	8.6 3 Compound: DNR hydrochloride	Cytotoxicity against SW620 colon cancer cell line done for 72 h at 37 degree C with compound in MTS assay Cytotoxicity against SW620 colon cancer cell line done for 72 h at 37 degree C with compound in MTS assay	[PMID: 16078845]
MCF7	IC₅₀	0.22 3 Compound: Daunorubicin	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 17190447]
T-24	IC₅₀	2 3 Compound: Daunomycin	Antiproliferative activity of compound was tested against bladder carcinoma (T-24) human tumor cells Antiproliferative activity of compound was tested against bladder carcinoma (T-24) human tumor cells	10.1016/0960-894X(95)00523-3
MCF7	IC₅₀	1.35 x 10^-5 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against MCF-7 cell line (breast carcinoma) after incubation for 72 hour Cytotoxicity of compound was tested against MCF-7 cell line (breast carcinoma) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
WiDr	IC₅₀	7 3 Compound: Daunomycin	Antiproliferative activity of compound was tested against colon carcinoma (WiDr) human tumor cells Antiproliferative activity of compound was tested against colon carcinoma (WiDr) human tumor cells	10.1016/0960-894X(95)00523-3
MCF7	IC₅₀	2 3 Compound: Daunomycin	Antiproliferative activity of compound was tested against mammary carcinoma (MCF-7) human tumor cells Antiproliferative activity of compound was tested against mammary carcinoma (MCF-7) human tumor cells	10.1016/0960-894X(95)00523-3
MCF7	IC₅₀	7.8 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against MCF-7 cell line (breast carcinoma) after incubation for 24 hour Cytotoxicity of compound was tested against MCF-7 cell line (breast carcinoma) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
MDA-MB-231	IC₅₀	0.25 3 Compound: Daunorubicin	Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay	[PMID: 17190447]
MOLT-4	IC₅₀	8.55 x 10^-13 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against Molt-4 cell line (T-cell leukemia) after incubation for 72 hour Cytotoxicity of compound was tested against Molt-4 cell line (T-cell leukemia) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
MOLT-4	IC₅₀	1.0 x 10^-9 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against Molt-4 cell line (T-cell leukemia) after incubation for 24 hour Cytotoxicity of compound was tested against Molt-4 cell line (T-cell leukemia) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
NHDF	IC₅₀	4.04 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against NHDF cell line (norm human dermal fibroblast) after incubation for 72 hour Cytotoxicity of compound was tested against NHDF cell line (norm human dermal fibroblast) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
NHDF	IC₅₀	3.9 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against NHDF cell line (norm human dermal fibroblast) after incubation for 24 hour Cytotoxicity of compound was tested against NHDF cell line (norm human dermal fibroblast) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
OVCAR-3	IC₅₀	2.87 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against Ovcar-3 cell line (ovarian carcinoma) after incubation for 72 hour Cytotoxicity of compound was tested against Ovcar-3 cell line (ovarian carcinoma) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
OVCAR-3	IC₅₀	3.9 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against Ovcar-3 cell line (ovarian carcinoma) after incubation for 24 hour Cytotoxicity of compound was tested against Ovcar-3 cell line (ovarian carcinoma) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
P388	IC₅₀	3 1 Compound: Daunorubicin HCl	Growth Inhibition of P388/0 cell lines. Growth Inhibition of P388/0 cell lines.	[PMID: 10669575]
P388	IC₅₀	< 9.8 x 10^-8 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against P-388 cell line (mouse leukemia) after incubation for 72 hour Cytotoxicity of compound was tested against P-388 cell line (mouse leukemia) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
P388	IC₅₀	3.9 x 10^-7 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against P-388 cell line (mouse leukemia) after incubation for 24 hour Cytotoxicity of compound was tested against P-388 cell line (mouse leukemia) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
PC-3	IC₅₀	0.23 6 Compound: Daunorubicin HCl	Cytotoxicity against human PC3 cells after 48 hrs by MTT assay Cytotoxicity against human PC3 cells after 48 hrs by MTT assay	[PMID: 12141872]
PC-3	IC₅₀	0.44 3 Compound: Daunorubicin	Cytotoxicity against human PC-3 cells after 48 hrs by MTT assay Cytotoxicity against human PC-3 cells after 48 hrs by MTT assay	[PMID: 17190447]
SK-MEL-28	IC₅₀	1.41 x 10^-6 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against SK-Mel-28 cell line (melanoma) after incubation for 72 hour Cytotoxicity of compound was tested against SK-Mel-28 cell line (melanoma) after incubation for 72 hour	10.1016/S0960-894X(00)80285-1
SK-MEL-28	IC₅₀	1.6 x 10^-6 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against SK-Mel-28 cell line (melanoma) after incubation for 24 hour Cytotoxicity of compound was tested against SK-Mel-28 cell line (melanoma) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
SW-620	IC₅₀	8.6 3 Compound: DNR hydrochloride	Cytotoxicity against SW620 colon cancer cell line done for 72 h at 37 degree C with compound in MTS assay Cytotoxicity against SW620 colon cancer cell line done for 72 h at 37 degree C with compound in MTS assay	[PMID: 16078845]
T-24	IC₅₀	2 3 Compound: Daunomycin	Antiproliferative activity of compound was tested against bladder carcinoma (T-24) human tumor cells Antiproliferative activity of compound was tested against bladder carcinoma (T-24) human tumor cells	10.1016/0960-894X(95)00523-3
UCLA P-3	IC₅₀	7.3 x 10^-3 41 Compound: 1 (Daunomycin hydrochloride)	Cytotoxicity of compound was tested against UCLA P-3 cell line (lung carcinoma) after incubation for 24 hour Cytotoxicity of compound was tested against UCLA P-3 cell line (lung carcinoma) after incubation for 24 hour	10.1016/S0960-894X(00)80285-1
WiDr	IC₅₀	7 3 Compound: Daunomycin	Antiproliferative activity of compound was tested against colon carcinoma (WiDr) human tumor cells Antiproliferative activity of compound was tested against colon carcinoma (WiDr) human tumor cells	10.1016/0960-894X(95)00523-3

体外実験

Daunorubicin hydrochloride (0-256 μg/mL, 30 min) inhibits DNA and RNA synthesis in sensitive and resistant Ehrlich ascites tumor cells^[2].
Daunorubicin hydrochloride (7 nM-1.9 μM, 72 h) shows chemosensitivity in Molt-4 cells and L3.6 cells^[3]^[4].
Daunorubicin hydrochloride (0.4 μM, 48 h) induces apoptotic and necrosis in L3.6 cells^[4].
Daunorubicin hydrochloride (0.4 μM, 120 min) induces ROS generation in L3.6 cells^[4].
Daunorubicin hydrochloride (2 μM, 24 h) induces autophagy in K562 cells (myeloid cell line)^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]^[4]

Cell Line:	Molt-4 cells (a human T-lymphoblastic leukemia cell line), L3.6 cells (metastatic human pancreatic cell line)
Concentration:	7 nM-1.9 μM
Incubation Time:	72 h
Result:	Inhibited cell viability with IC₅₀ values of 40 nM (Molt-4) and 400 nM (L3.6).

Apoptosis Analysis^[4]

Cell Line:	L3.6 cells
Concentration:	0.4 μM
Incubation Time:	72 h
Result:	Induced necrosis without apoptosis at 24 h, induced both an apoptotic and extensive necrotic response at 48 h.

Western Blot Analysis^[6]:

Cell Line:	K562 cells
Concentration:	2 μM
Incubation Time:	24 h
Result:	Enabled the switch of LC3-I into LC3-II, accompanied with a significant increased expression level of LC3.

体内実験

Daunorubicin hydrochloride (intravenous injection, 3 mg/kg, three times at 48 h intervals.) produces cardiotoxicity and nephrotoxicity in rats^[5].
Daunorubicin hydrochloride (intraperitoneal injection, 10 mg/kg) induces sister chromatid exchanges in mice^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague-Dawley rats^[5]
Dosage:	3 mg/kg
Administration:	Intravenous injection, three times at 48 h intervals.
Result:	Caused a significant increase in MDA (malondialdehyde) level in renal tissue, accompanied by a significant reduction in total GPx activity. Increased urinary protein excretion, serum creatinine, and BUN level.

分子量

563.98

分子式

C₂₇H₃₀ClNO₁₀

CAS 番号

23541-50-6

Appearance

Solid

Color

Pink to red

SMILES

O=C(C(C(OC)=CC=C1)=C1C2=O)C3=C2C(O)=C(C[C@@](O)(C(C)=O)C[C@@H]4O[C@@]5([H])C[C@H](N)[C@H](O)[C@H](C)O5)C4=C3O.[H]Cl

Structure Classification

Initial Source

Microorganisms

Streptomyces coeruleorubidus

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶剤 & 溶解度

体外:

DMSO : 50 mg/mL (88.66 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 34 mg/mL (60.29 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7731 mL	8.8656 mL	17.7311 mL
5 mM	0.3546 mL	1.7731 mL	3.5462 mL
10 mM	0.1773 mL	0.8866 mL	1.7731 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.69 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.69 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 33.33 mg/mL (59.10 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

純度とドキュメンテーション

純度: 99.67%

Select Batch:

データシート (287 KB) SDS (644 KB)

COA (258 KB) HNMR (135 KB) LCMS (93 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Lehmann M, et al. Activity of topoisomerase inhibitors daunorubicin, idarubicin, and aclarubicin in the Drosophila Somatic Mutation and Recombination Test. Environ Mol Mutagen. 2004;43(4):250-7. [Content Brief]

[2]. Dano K, et al. Inhibition of DNA and RNA synthesis by daunorubicin in sensitive and resistant Ehrlich ascites tumor cells in vitro. Cancer Res. 1972 Jun;32(6):1307-14. [Content Brief]

[3]. Svensson SP, et al. Melanin inhibits cytotoxic effects of Doxorubicin and Daunorubicin in MOLT 4 cells. Pigment Cell Res. 2003 Aug;16(4):351-4 [Content Brief]

[4]. Gervasoni JE Jr, et al. An effective in vitro antitumor response against human pancreatic carcinoma with paclitaxel and Daunorubicin by induction of both necrosis and apoptosis. Anticancer Res. 2004 Sep-Oct;24(5A):2617-26 [Content Brief]

[5]. Arozal W, et al. Telmisartan prevents the progression of renal injury in daunorubicin rats with the alteration of angiotensin II and endothelin-1 receptor expression associated with its PPAR-γ agonist actions. Toxicology. 2011 Jan 11;279(1-3):91-9. [Content Brief]

[6]. Emeline Bollaert, et al. MiR-15a-5p Confers Chemoresistance in Acute Myeloid Leukemia by Inhibiting Autophagy Induced by Daunorubicin. Int J Mol Sci. 2021 May 13;22(10):5153. [Content Brief]

[7]. Cheng Wu, et al. Doxorubicin suppresses chondrocyte differentiation by stimulating ROS production. Eur J Pharm Sci. 2021 Dec 1;167:106013. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.7731 mL	8.8656 mL	17.7311 mL	44.3278 mL
	5 mM	0.3546 mL	1.7731 mL	3.5462 mL	8.8656 mL
	10 mM	0.1773 mL	0.8866 mL	1.7731 mL	4.4328 mL
	15 mM	0.1182 mL	0.5910 mL	1.1821 mL	2.9552 mL
	20 mM	0.0887 mL	0.4433 mL	0.8866 mL	2.2164 mL
	25 mM	0.0709 mL	0.3546 mL	0.7092 mL	1.7731 mL
	30 mM	0.0591 mL	0.2955 mL	0.5910 mL	1.4776 mL
	40 mM	0.0443 mL	0.2216 mL	0.4433 mL	1.1082 mL
	50 mM	0.0355 mL	0.1773 mL	0.3546 mL	0.8866 mL
	60 mM	0.0296 mL	0.1478 mL	0.2955 mL	0.7388 mL
DMSO	80 mM	0.0222 mL	0.1108 mL	0.2216 mL	0.5541 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Daunorubicin hydrochloride Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

日本の代理先をお選びくださいナミキ商事株式会社コスモ・バイオ株式会社重松貿易株式会社
製品名			カスタマ需要量 *
お名前 *			タイトル
メールアドレス *			電話番号 *
デパートメント			組纖名 *
市区町村			都道府県
国或いは地域 *
必ず会社名を記載ください。個人への返信は行いません。
備考

製品名			Lot #
お名前 *			メールアドレス *
電話番号 *			部門 *
組纖名 *			国或いは地域 *
備考

ダウノルビシン塩酸塩 (Synonyms: Daunorubicin (hydrochloride); Rubidomycin (hydrochloride); Daunomycin (hydrochloride); RP 13057 (hydrochloride))

カスタマーレビュー

Other Forms of Daunorubicin hydrochloride:

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Topoisomerase アイソフォーム固有の製品をすべて表示:

DNA/RNA Synthesis アイソフォーム固有の製品をすべて表示:

ADC Payload アイソフォーム固有の製品をすべて表示:

Antibiotic アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Daunorubicin hydrochloride Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

お名前
メール *

	Sorry, but the email address you supplied was invalid.

ダウノルビシン塩酸塩 (Synonyms: Daunorubicin (hydrochloride); Rubidomycin (hydrochloride); Daunomycin (hydrochloride); RP 13057 (hydrochloride))

カスタマーレビュー

Other Forms of Daunorubicin hydrochloride:

ダウノルビシン塩酸塩 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Topoisomerase アイソフォーム固有の製品をすべて表示:

DNA/RNA Synthesis アイソフォーム固有の製品をすべて表示:

ADC Payload アイソフォーム固有の製品をすべて表示:

Antibiotic アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

ダウノルビシン塩酸塩 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Daunorubicin hydrochloride Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

カスタマーレビュー

Request for HNMR Report

Request for HNMR Report

ダウノルビシン塩酸塩関連抗体

ダウノルビシン塩酸塩関連抗体