2. Others NF-κB Apoptosis Anti-infection
  3. Parasite NF-κB Environmental Pollutants Bcl-2 Family Caspase Apoptosis
  4. Azadirachtin

アザジラクチン  (Synonyms: Azadirachtin)

製品番号: HY-126741 純度: 99.06%
COA 取扱説明書 Technical Support

Azadirachtin is an oral active triterpenoid compound with anticancer, antimalarial, anti-inflammatory, and insecticidal activities. Azadirachtin induces cell apoptosis through the mitochondrial pathway (by inhibiting Bcl-2/Bax ratio or activating Apaf-1 and caspase-3) or through death receptors (by inhibiting TNFR activation). Additionally, Azadirachtin exerts its anti-inflammatory effects by inhibiting NF-кB signaling pathway activation, and it exhibits insecticidal activity by inducing apoptosis in insect cells.

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Azadirachtin

アザジラクチン 構造式

CAS 番号 : 11141-17-6

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 436 在庫あり
Solution
10 mM * 1 mL in DMSO USD 436 在庫あり
Solid
1 mg $72 在庫あり
5 mg $275 在庫あり
10 mg $420 在庫あり
50 mg   お問い合わせ  
100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 2 publication(s) in Google Scholar

Other Forms of Azadirachtin:

アザジラクチン 関連抗体

Top Publications Citing Use of Products

Parasite アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

NF-κB アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

Bcl-2 Family アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3 Bad

Caspase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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製品説明

Azadirachtin is an oral active triterpenoid compound with anticancer, antimalarial, anti-inflammatory, and insecticidal activities. Azadirachtin induces cell apoptosis through the mitochondrial pathway (by inhibiting Bcl-2/Bax ratio or activating Apaf-1 and caspase-3) or through death receptors (by inhibiting TNFR activation). Additionally, Azadirachtin exerts its anti-inflammatory effects by inhibiting NF-кB signaling pathway activation, and it exhibits insecticidal activity by inducing apoptosis in insect cells[1][2][3][4][5][6].

IC50 & Target

Caspase 3

 

体外実験

Azadirachtin (3-24 μM, 6 h) exerts its anti-inflammatory effects in Retinoic acid (HY-14649)-induced A549 (lung carcinoma) cells by inhibiting the NF-κB signaling pathway[3].
Azadirachtin (135 μM, 24 h) induces cell cycle arrest and mitochondria-mediated apoptosis in human cervical cancer (HeLa) cells[5].
Azadirachtin (50 μg/mL, 22-24 h) inhibits the early sporogonic development of Plasmodium berghei and blocks its transmission effect[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

アザジラクチン 関連抗体

Western Blot Analysis[3]

Cell Line: Retinoic acid (HY-14649) -induced human lung carcinoma (A549) cells
Concentration: 0, 3, 6, 12, 24 μM
Incubation Time: 6 h
Result: Inhibited Retinoic acid (HY-14649)-induced NF-κB-dependent gene (ICAM1 (intercellular adhesion molecule 1) and cox2) expression
体内実験

Azadirachtin (10 μg/kg, i.g., once daily, 3 days a week for 14 weeks) carcinogenesis in the DMBA (HY-W011845)-induced hamster buccal pouch (HBP) carcinoma model by modulating xenobiotic metabolism enzymes, DNA damage, antioxidants, invasion, and angiogenesis.[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: DMBA (HY-W011845)-induced hamster buccal pouch (HBP) carcinoma model[4]
Dosage: 10 μg/kg
Administration: Intragastric administration (i.g.), once daily, 3 days a week for 14 weeks
Result: Significantly decreased the expression of CYP1A1, CYP1B1 and 8-OHdG and up-regulated RECK expression.
Significantly enhanced GSH and the activities of all the antioxidant enzymes.
Decreased phase I enzyme activities and elevated phase II enzyme activities in the pouch.
臨床実験
分子量

720.71

分子式

C35H44O16
CAS 番号
Appearance

Solid

Color

White to light yellow

SMILES

C[C@@]1([C@@]([C@@]2(O)C(OC)=O)([H])[C@]([C@H](C[C@H]3OC(C)=O)OC(/C(C)=C/C)=O)(CO2)[C@@]4([H])[C@](OC[C@@]43C(OC)=O)([H])[C@H]1O)[C@]([C@@H](O[C@]5([H])OC=C6)C[C@H]7[C@]56O)(O8)[C@]78C
Structure Classification
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
体外: 

DMSO : ≥ 100 mg/mL (138.75 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3875 mL 6.9376 mL 13.8752 mL
5 mM 0.2775 mL 1.3875 mL 2.7750 mL
10 mM 0.1388 mL 0.6938 mL 1.3875 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

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体積 （開始）

V1

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体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (3.47 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (3.47 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.06%

COA (263 KB) LCMS (86 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3875 mL 6.9376 mL 13.8752 mL 34.6880 mL
5 mM 0.2775 mL 1.3875 mL 2.7750 mL 6.9376 mL
10 mM 0.1388 mL 0.6938 mL 1.3875 mL 3.4688 mL
15 mM 0.0925 mL 0.4625 mL 0.9250 mL 2.3125 mL
20 mM 0.0694 mL 0.3469 mL 0.6938 mL 1.7344 mL
25 mM 0.0555 mL 0.2775 mL 0.5550 mL 1.3875 mL
30 mM 0.0463 mL 0.2313 mL 0.4625 mL 1.1563 mL
40 mM 0.0347 mL 0.1734 mL 0.3469 mL 0.8672 mL
50 mM 0.0278 mL 0.1388 mL 0.2775 mL 0.6938 mL
60 mM 0.0231 mL 0.1156 mL 0.2313 mL 0.5781 mL
80 mM 0.0173 mL 0.0867 mL 0.1734 mL 0.4336 mL
100 mM 0.0139 mL 0.0694 mL 0.1388 mL 0.3469 mL
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Azadirachtin Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Azadirachtin11141-17-6ParasiteNF-κBEnvironmental PollutantsBcl-2 FamilyCaspaseApoptosisNuclear factor-κBNuclear factor-kappaBEnvironmental ContaminantsinsecticidespestcytoskeletonsignaltransductioncellularmetabolismsInhibitorinhibitorinhibit

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