Cell Culture

MCE PolyFast Transfection Reagent consists of cationic polymers and can introduce nucleic acids (DNA or RNA) into eukaryotic cells efficiently, including some hard-to-transfect cells.

MCE PEI Transfection Reagent is designed based on 25 kDa PEI. It has high-efficiency, low-toxicity, strong-stability, and is suitable for many cell types, such as HEK-293、HEK-293T、CHO-K1、COS-1、COS-7、NIH/3T3、Sf9、HepG2 and HeLa et, even some hard-to-transfect cells. It can also be applied to large-scale recombinant protein expression and virus production. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE Lentivirus Transfection Reagent is a new type of transfection reagent based on cationic polymer, which is suitable for lentivirus packaging and transfection. It significantly improves the efficiency of packaging and transfection, resulting in the production of more recombinant lentivirus.

MCE Protein Transfection Reagent is a cationic lipid mixture for complexation with proteins, peptides, antibodies and other biologically active molecules to allow their direct intracellular delivery.

MCE siRNA/miRNA Transfection Reagent is a novel cationic polymer transfection reagent for siRNA and miRNA transfection. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE OptiLNP RNA Transfection Reagent is a ready-to-use transfection reagent based on LNP technology. It is designed for the efficient transfection of various RNA types into common cells.

MCE OptiLNP RNA Transfection Reagent (Stem Cells) is a ready-to-use transfection reagent based on LNP technology. It is designed for the efficient transfection of various RNA types into stem cells.

MCE OptiLNP RNA Transfection Reagent (Primary Immune Cells) is a ready-to-use transfection reagent based on LNP technology. It is designed for the efficient transfection of various RNA types into primary immune cells.

MCE OptiLNP RNA Transfection Reagent (Immune Cells) is a ready-to-use transfection reagent based on LNP technology. It is designed for the efficient transfection of various RNA types into immune cells.

MCE In vivo OptiLNP RNA Transfection Reagent (Systemic Effect) is a ready-to-use transfection reagent based on LNP technology. It is designed for the efficient transfection of mRNA and short-chain RNA in experimental animals. The RNA functions systemically.

MCE In vivo OptiLNP RNA Transfection Reagent (Local Effect) is a ready-to-use transfection reagent based on LNP technology. It is designed for the efficient transfection of mRNA and short-chain RNA in experimental animals. The RNA functions locally.

MCE In vivo OptiLNP RNA Transfection Reagent (Liver-targeted) is a ready-to-use transfection reagent based on LNP technology. It is designed for the efficient transfection of mRNA and short-chain RNA via intravenous injection in experimental animals.

MCE In vivo OptiLNP RNA Transfection Reagent (Lung-targeted) is a ready-to-use transfection reagent based on LNP technology. It is designed for the efficient transfection of mRNA and short-chain RNA via intravenous injection in experimental animals.

MCE OptiLNP Short-Chain RNA Transfection Reagent is a ready-to-use transfection reagent based on LNP technology. It is designed for the efficient transfection of short-chain RNA into common cells.

MCE OptiLNP Gene Editing Kit is a ready-to-use transfection reagent based on LNP technology. It is designed for the efficient co-transfection of Cas9 mRNA and sgRNA during gene editing in common cells (adherent or suspension cells).

MCE OptiLNP Gene Editing Kit (Immune Cells) is a ready-to-use transfection reagent based on LNP technology. It is designed for the efficient co-transfection of Cas9 mRNA and sgRNA during gene editing inimmune cells.

MCE HP PolyFast Transfection Reagent is a novel cationic polymer transfection reagent for DNA and RNA transfection.

MCE BM-Cyclin mainly contains Tiamulin fumarate and Minocycline hydrochloride, can effectively inhibit and remove mycoplasma contamination widespread in cell cultures. It is also effective against some common bacteria.

MCE Penicillin-Streptomycin-Neomycin (PSN) (100×), Sterile is used to prevent bacterial contamination of cell culture due to their effective combined action against gram-positive and gram-negative bacteria, which contains 5,000 units/mL of penicillin, 5 mg/mL of streptomycin, and 10 mg/mL of Neomycin.

MCE Penicillin-Streptomycin (100×), Sterile is used to prevent bacterial contamination of cell culture due to their effective combined action against gram-positive and gram-negative bacteria, which contains 10,000 units/mL of penicillin and 10 mg/mL of streptomycin.

MCE Gentamicin-Amphotericin B (500×), sterile is used to prevent contamination of cell culture due to their effective combined action against gram-positive bacteria, gram-negative bacteria, fungi and yeasts, which contains 5 mg/mL of gentamicin, and 125 μg/mL of amphotericin.

MCE Penicillin-Streptomycin-Glutamine (100×), sterile is used to prevent bacterial contamination of cell culture due to their effective combined action against gram-positive and gram-negative bacteria, which contains 10,000 units/mL of penicillin, 10 mg/mL of streptomycin, and 29.2 mg/mL of Glutamine.

Polymixin B is a mixture of B1 and B2 polypeptides obtained from different strains of Bacillus polymyxa, with antibacterial activity against gram-negative bacteria. It can bind lipopolysaccharide (LPS) of the outer membrane of gram-negative bacteria and disrupts the cytoplasmic membrane by inducing large pores to allow nucleotide leakage in bacterial walls. This disrupts the permeability of the cytoplasmic membrane.

MCE Gentamicin, Sterile (10 mg/mL) is a filtered and sterilized antibiotic solution that can be used directly in cell culture. Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria, mainly for gram-negative and gram-positive bacteria.

MCE Hygromycin B, Sterile (50 mg/mL) is a filtered and sterilized antibiotic solution that can be used directly in cell culture.Hygromycin B is an aminoglycosidic antibiotic purified from Streptomyces hygroscopicus. It acts by binding to the 70S subunit of the bacterial ribosome and inhibiting protein synthesis, leading to the death of bacteria, fungi and mammalian cells.

MCE Amphotericin B, Sterile (250 μg/mL) is a filtered and sterilized antibiotic solution that can be used directly in cell culture. Amphotericin B is a polyene antifungal antibiotic produced by Streptomyces nodosus. It can bind with sterols to form transmembrane channels, resulting in the leakage of intracellular substances, thus inhibiting fungal and yeast contamination.

MCE Blasticidin S, Sterile (10 mg/mL) is a filtered and sterilized antibiotic solution that can be used directly in cell culture. Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation.

Kanamycin sulfate is an aminoglycoside antibiotic purified from Streptomyces kanamyceticus. It acts by binding to the 30S subunit of the bacterial ribosome and inhibiting protein synthesis in susceptible bacteria.

MCE G418 Selective Antibiotic, Sterile (50 mg/mL) is an aminoglycoside antibiotic produced by Micromonospora rhodorangea. It acts by binding to the 80S subunit of the bacterial ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells.

Puromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It inhibits protein synthesis by disrupting peptide transfer on ribosomes, causing premature chain termination during translation. It can kill most gram-positive bacteria and various animal or insect cells.

MCE Antibiotic-Antifungal (100 ×), Sterile contains 10 KU/mL of penicillin, 10 mg/mL of streptomycin, and 25 μg/mL of Amphotericin B. The antibiotics penicillin and streptomycin prevent bacterial contamination, and Amphotericin B prevents fungal contamination.

MCE Serum/Protein-Free Cell Freezing Medium is a complete ready-to-use cryopreservation medium. It is suitable for the cryopreservation of conventional mammalian cells and serum-free cultured cells.

MCE CEPT Cocktail is composed of CET small molecules (Chroman 1, Emricasan and Trans-ISRIB) and Polyamine Solution, and can effectively enhance cell survival and promote clonal growth of genetically stable hPSCs.

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cell lines successfully cultured in DMEM include Hela, 293, Cos-7, and PC-12, as well as primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. The 500 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cell successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, etc. The 500 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cell lines successfully cultured in DMEM include Hela, 293, Cos-7, and PC-12, as well as primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, etc. The 500 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

RPMI 1640 contains glutathione and high concentration of vitamins, also contains biotin, vitamin B12, 4-Aminobenzoic acid (PABA) not found in MEM and DMEM, as well as high concentration of inositol and choline chloride. The 500 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Minimum Essential Medium (MEM), also known as Minimal Essential Medium, was developed by Harry Eagle based on Eagle’s Basal Medium (BME). It is a basic cell culture medium with a simple formulation and broad applicability, and is one of the most commonly used media in animal cell culture. MEM contains only 12 essential amino acids, L-glutamine, and 8 vitamins. It is primarily used for the culture of adherent cells. With appropriate formulation modifications, it can also be used for other types of cell culture.

MCE Advanced DMEM/F-12 is an optimized mammalian cell culture medium designed to reduce the requirement for fetal bovine serum (FBS). Compared with classical DMEM/F-12 medium, it can reduce serum usage by 50–90% while maintaining comparable cell growth rates and cellular morphology, thereby effectively lowering serum consumption and reducing overall cell culture costs.

Ham’s F-12K Medium was originally designed for the culture of primary human hepatocytes and differentiated rat and chicken cells. It is particularly suitable for single-cell cloning of Chinese hamster ovary (CHO) cells under serum-free conditions. When supplemented with serum, it can also be used for the culture of various other mammalian cell types, including chondrocytes and rat prostate epithelial cells.

Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells, diluting cells and preparing reagents. The 500 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells, diluting cells and preparing reagents. The 500 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells, diluting cells and preparing reagents. The 500 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Hanks’ Balanced Salt Solution (HBSS) is widely used in various cell culture applications, including washing cells prior to dissociation, transporting cells or tissue samples, diluting cells for cell counting, and preparing a variety of reagent solutions. Formulations without calcium and magnesium are suitable for washing chelating agents prior to cell dissociation procedures. The 500 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Hanks’ Balanced Salt Solution (HBSS) is widely used in various cell culture applications, including washing cells prior to dissociation, transporting cells or tissue samples, diluting cells for cell counting, and preparing a variety of reagent solutions. Formulations without calcium and magnesium are suitable for washing chelating agents prior to cell dissociation procedures. The 500 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Hanks’ Balanced Salt Solution (HBSS) is widely used in various cell culture applications, including washing cells prior to dissociation, transporting cells or tissue samples, diluting cells for cell counting, and preparing a variety of reagent solutions. Formulations without calcium and magnesium are suitable for washing chelating agents prior to cell dissociation procedures. The 500 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Hanks’ Balanced Salt Solution (HBSS) is widely used in various cell culture applications, including washing cells prior to dissociation, transporting cells or tissue samples, diluting cells for cell counting, and preparing a variety of reagent solutions. Formulations without calcium and magnesium are suitable for washing chelating agents prior to cell dissociation procedures. The 500 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

L-glutamine is an important amino acid supplement commonly added to mammalian cell culture media. L-glutamine serves as an auxiliary energy source, especially when cells are rapidly dividing. L-glutamine is also important in the production of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, other amino acids, and plays a role in protein synthesis and glucose production.

MCE L-Alanyl-L-Glutamine (100×) contains 200 mM L-alanyl-L-glutamine and is formulated in 0.85% NaCl. It serves as a stable dipeptide substitute for L-glutamine. Compared with free L-glutamine, it exhibits superior stability and significantly reduces the accumulation of toxic ammonia during culture, thereby improving cell viability and growth performance.

MEM Non-essential Amino Acid Solution (100×) has been filtered and can be used directly. It adds nutrients and reduces the biosynthetic burden on cell in vitro. The 100 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

This product is serum-free and supports cell growth and survival. It has been associated with the growth and expression of neuroblastoma, and with post-mitotic neuronal survival and expression in primary cultures of both the peripheral nervous system (PNS) and central nervous system (CNS). This product is formulated with water-for-injection. The 5 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE N-2 Serum-free Supplement (100×) can be used in conjunction with growth factors such as bFGF and EGF in serum-free neuronal cell culture mediums, DMEM and the DMEM/F12. This product is formulated with basal culture medium. The 5 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

This product is the serum-free B-27 Supplement, the most widely cited neuronal culture supplement. It is an optimized formulation designed to support both low- and high-density growth, as well as short- and long-term viability of embryonic neurons. This product is formulated with water-for-injection. The 10 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE Bi-27 Serum-free Supplement (50×) can be used to support the low- or high-density growth and short- or long-term viability of embryonic. This product is formulated with basal culture medium. The 10 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

This product is serum-free and vitamin A-free.It is a customized variant of the standard Bi-27 formulation with vitamin A removed as a supplement for neuronal cell culture to support the low- or high-density growth and short- or long-term viability of embryonic. This product is formulated with water-for-injection. The 10 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE Bi-27 Serum-free Supplement (50×), Vitamin A-Free can be used to support the low- or high-density growth and short- or long-term viability of embryonic. This product is formulated with basal culture medium. The 10 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE Bi-27 Serum-free Supplement (50×), Insulin-Free is a customized formulation developed by removing insulin from the standard Bi-27 formulation. As a neuronal cell culture supplement, it is specifically designed to support the growth and maintenance of hippocampal neurons and other central nervous system (CNS) neurons in applications where the presence of insulin may interfere with experimental outcomes.

MCE Bi-27 Serum-free Supplement (50×), Antioxidant-Free is a customized neuronal culture supplement developed by removing antioxidants components (such as vitamins, superoxide dismutase, catalase, etc.) from the standard Bi-27 formulation. It is specifically designed for investigating the roles of reactive oxygen species (ROS) in aging, cytotoxicity, apoptosis, and chronic neurological diseases. This formulation provides a stable culture environment for mechanistic studies without the interference of exogenous antioxidants.

MCE HAT Supplement (50×) can be added to cell culture medium as a selection agent for post-fusion selection of HGPRT-myeloma hybridoma cells used for antibody production. The 10 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE HT Supplement (50×) is a liquid mixture of hypoxanthine (5 mM) and thymidine (0.8 mM), and can be added to cell culture medium as an additive for the DNA salvage synthesis pathway. The 10 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE ITS Supplement (100×) can be used as a cell culture supplement and added to the medium for serum-free or low-serum cell culture systems.

MCE Lipid Concentrate (Chemically Defined) is a concentrated lipid solution with a clearly defined chemical composition, which can be added to cell culture media as an additive for serum-free or low-serum cell culture systems.

MCE Recombinant Human Serum Albumin Solution (rHSA, 20%) can be widely used in various cell culture systems and is particularly suitable for maintaining the undifferentiated growth and expansion of human embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC) under both feeder-dependent and feeder-free conditions.

MCE Sodium Pyruvate Solution (100 mM) contains 100 mM (11 g/L) sodium pyruvate and is ready for direct use in cell culture applications. The recommended working concentration is 1 mM, corresponding to a 1:100 dilution prior to use.

MCE D-Glucose Solution (200 g/L) can be used as a serum-free supplement for cell culture media and is suitable for a variety of culture systems, including but not limited to DMEM, RPMI 1640, DMEM/F-12, IMDM, and MEM.

MCE MEM Vitamin Solution (100×) contains eight vitamins, including choline chloride, D-calcium pantothenate, folic acid, nicotinamide, pyridoxal hydrochloride, riboflavin, thiamine hydrochloride, and inositol. It can be used as a serum-free supplement for cell culture media to enhance cell viability and promote cell growth.

MCE Exosome-Specific 293 Cell Medium Supplement is designed as a feed supplement for use with the Exosome-Specific 293 Cell Medium (Serum-Free) (MCE Cat. No.: HY-K3113).

MCE 0.25% Trypsin-EDTA (1x), phenol red consists of dried trypsin powder dissolved in EDTA. MCE Trypsin hydrolyzes intercellular proteins and disperses primary tissues or adherent cells into individual cells, and can be widely used for cell dissociation, cell culture passaging and primary tissue dissociation. The 100 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE 0.25% Trypsin-EDTA (1×), Phenol Red-Free, is prepared by dissolving trypsin powder (a mixture of proteases from porcine pancreas, irradiated and sterilized) and EDTA in a calcium- and magnesium-free balanced salt solution. It is filter-sterilized and can be directly used for the dissociation of cultured cells and tissues. The 100 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE Red Blood Cell Lysis Buffer (10×) primarily contains ammonium chloride and is a ready-to-use solution designed for rapid and effective lysis and removal of anucleated red blood cells from human or mouse blood and tissue samples without affecting white blood cells, normal tissues, or tumor cells.

MCE iPSC/ESC Dissociation Solution (Enzyme-Free) is a chelation-based, enzyme-free cell dissociation reagent specifically designed for the culture and manipulation of induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs). This optimized solution enables gentle and efficient dissociation of iPSCs/ESCs within 5-8 min, making it suitable for routine passaging, dissociation, and cell-cluster handling. It provides superior stability and better preservation of cell state compared with traditional methods.

MCE ECM Gentle Dissociation Solution is a gentle ECM-degrading enzyme mixture derived from marine bacteria and Bacillus species, specifically formulated for efficient and low-damage digestion of in-vitro cell systems. It selectively degrades extracellular matrix components while minimizing disruption to the cell membrane and intercellular junctions, thereby significantly reducing mechanical stress during dissociation. This product is compatible with a wide range of cell types, including stem cell colonies, primary cells, neural cells, and organoids, and is particularly well suited for gentle yet effective dissociation of brain organoids and other complex 3D structures.

MCE ECM Gentle Dissociation Solution is a gentle ECM-degrading enzyme mixture derived from marine bacteria and Bacillus species, specifically formulated for efficient and low-damage digestion of in-vitro cell systems. It selectively degrades extracellular matrix components while minimizing disruption to the cell membrane and intercellular junctions, thereby significantly reducing mechanical stress during dissociation.

Density Gradient Medium, also known as Iodixanol, is a low osmolality, non-ionic, iodinated density gradient medium.

MCE Cell Separation Medium (Density Gradient) is a low-density gradient medium characterized by its broad applicability, operational simplicity, and gentle separation performance.It is primarily composed of silica particles (15-30 nm in diameter) coated with a monolayer of polyvinylpyrrolidone (PVP).

MCE Human Neuroblastoma Cell Culture Medium is specially designed to promote the in vitro growth of primary human neuroblastoma cells.

MCE Human Acute Myeloid Leukemia Cell Culture Medium is specially designed to promote the in vitro growth of primary human acute myeloid leukemia cells.

MCE T-Cell Expansion Medium (Serum-Free) is a specialized culture medium formulated for the in vitro cultivation and robust expansion of human T lymphocytes without the need for serum.

MCE Exosome-Specific Culture Medium (Serum-Free, Phenol Red-Free) is specifically formulated for optimized exosome collection.

MCE Exosome-Specific Mesenchymal Stem Cell Medium (Serum-Free, Phenol Red-Free) is specifically optimized for the production and preparation of exosomes from mesenchymal stem cells (MSCs).

MCE Exosome-Specific 293 Cell Medium (Serum-Free) is specifically optimized for exosome production from HEK293 cells.

MCE Human PSC Maintenance Medium is a ready-to-use, chemically defined formulation designed to support the robust growth and self-renewal of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) under feeder-free culture conditions.

MCE Lentivirus Solution Buffer can be uesd for resuspending lentiviral particles after ultracentrifugation, PEG precipitation, or ultrafiltration concentration.

MCE Lentivirus Transfection Reagent is a new type of transfection reagent based on cationic polymer, which is suitable for lentivirus packaging and transfection. It significantly improves the efficiency of packaging and transfection, resulting in the production of more recombinant lentivirus.

MCE Lentivirus Packaging Kit is based on the second-generation lentiviral packaging system and is also compatible with both second- and third-generation packaging plasmids. Lentiviruses produced using this kit exhibit high infection efficiency and a broad host range, enabling stable and sustained expression of exogenous genes in host cells.

MCE Lentivirus Concentration Solution (5×) is a high-efficiency polymer solution optimized for lentiviral particle enrichment, providing a simple, rapid, and effective method for lentivirus concentration.