ベンゾブロマロン (Synonyms: Benzbromarone)

Name: Benzbromarone
Brand: MedChemExpress
SKU: HY-B1135
Rating: 5.0 (9 reviews)

製品番号: HY-B1135 純度: 99.91%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

Benzbromarone is an orally active anti-gout agent. Benzbromarone has anti-infammatory, anti-oxidative stress and nephroprotective effects. Benzbromarone can be used for the research of hyperuricemia and gout.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 3562-84-3

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 66	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 66	在庫あり	Estimated Time of Arrival: December 31
Solid
100 mg	$60	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 9 publication(s) in Google Scholar

Other Forms of Benzbromarone:

Benzbromarone-d₅ 在庫あり
Benzbromarone (Standard) 在庫あり

顧客検証

Cell Proliferation/Viability Assay

Bio/Physico-chemical Assay

In Vivo Efficacy Study

: Benzbromarone purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Dec;15(12):6382-6398.; Inhibition of ¹⁴C-urate transport by Benzbromarone in URAT1^EM and its mutants.

: Benzbromarone purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Aug;10(24):e2300881. [Abstract]; Benzbromarone (BBR) (1-100 μM; 72 h) inhibited in vitro proliferation of multiple human GC and CRC cell lines.

: Benzbromarone purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Aug;10(24):e2300881. [Abstract]; Benzbromarone (BBR) (50 mg/kg; p.o.; once every other day for 3 weeks) showed anti-tumor effectiveness in ANO1-positive GC PDX mice.

: Benzbromarone purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Aug;10(24):e2300881. [Abstract]; Benzbromarone (BBR) (50 mg/kg; p.o.; once every other day for 3 weeks) evidently augmented the anti-tumor effect of anti-PD-1 antibody against MC38/CT26 xenograft mice.

: Benzbromarone purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Aug;10(24):e2300881. [Abstract]; Benzbromarone (BBR) (1-100 μM) dose-dependently inhibited ANO1's protein expression in multiple GI cancer cells.

Interleukin Related アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

Caspase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

Bcl-2 Family アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3 Bad

NF-κB アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

JNK アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

JNK1 JNK2 JNK3 JNK

HSP アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

HSP40 HSP70 HSP90 HSP105 HSF1 HSP HSPA5

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

IC₅₀ & Target

IL-1β

IL-8

Caspase-8

Caspase 9

Caspase 3

Bcl-2

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	0.3 μM Compound: benzbromarone	Inhibition of human URAT1-mediated urate uptake in HEK293 cells Inhibition of human URAT1-mediated urate uptake in HEK293 cells	[PMID: 17325024]
HEK-293T	IC₅₀	0.12 μM Compound: 2	Inhibition of human URAT1 expressed in HEK293T cells assessed as reduction in [14C]uric acid uptake measured after 5 mins by liquid scintillation counting method Inhibition of human URAT1 expressed in HEK293T cells assessed as reduction in [14C]uric acid uptake measured after 5 mins by liquid scintillation counting method	[PMID: 28351592]
MDCK	EC₅₀	39 μM Compound: 21	Antiviral activity against Influenza A virus A/PR/8/34 (H1N1) infected in MDCK cells assessed as reduction in plaque formation after 2 days by plaque reduction assay Antiviral activity against Influenza A virus A/PR/8/34 (H1N1) infected in MDCK cells assessed as reduction in plaque formation after 2 days by plaque reduction assay	[PMID: 27046062]
MDCK	EC₅₀	39 μM Compound: 3	Antiviral activity against Influenza A virus (A/PR/8/34) (H1N1) infected in MDCK cells assessed as reduction in plaque formation after 48 hrs by plaque reduction assay Antiviral activity against Influenza A virus (A/PR/8/34) (H1N1) infected in MDCK cells assessed as reduction in plaque formation after 48 hrs by plaque reduction assay	[PMID: 25856229]
MDCK	IC₅₀	35 nM Compound: 7, benzbromarone	Inhibition of human URAT1 expressed in human MDCK cells Inhibition of human URAT1 expressed in human MDCK cells	[PMID: 21449597]
Oocyte	IC₅₀	22 μM Compound: Benzobromaron	TP_TRANSPORTER: inhibition of lactate uptake in Xenopus laevis oocytes TP_TRANSPORTER: inhibition of lactate uptake in Xenopus laevis oocytes	[PMID: 10417314]
Oocyte	IC₅₀	9 μM Compound: Benzobromaron	TP_TRANSPORTER: inhibition of lactate uptake in Xenopus laevis oocytes TP_TRANSPORTER: inhibition of lactate uptake in Xenopus laevis oocytes	[PMID: 10417314]
RPTEC	IC₅₀	6.8 μM Compound: BBR	Inhibition of uric acid uptake in human RPTEC Inhibition of uric acid uptake in human RPTEC	[PMID: 33684442]
S2	IC₅₀	4.6 μM Compound: Benzbromarone	TP_TRANSPORTER: inhibition of Urate uptake (Urate: 300 uM) in OAT1-expressing S2 cells TP_TRANSPORTER: inhibition of Urate uptake (Urate: 300 uM) in OAT1-expressing S2 cells	[PMID: 12472777]
Sf9	IC₅₀	4 μM Compound: Benzbromarone	Inhibition of human MRP1 expressed in Spodoptera frugiperda Sf9 cells assessed as inhibition of NEM-GS-induced vanadate-sensitive ATPase activity measured by generation of inorganic phosphate by spectrophotometry in presence of glutathione Inhibition of human MRP1 expressed in Spodoptera frugiperda Sf9 cells assessed as inhibition of NEM-GS-induced vanadate-sensitive ATPase activity measured by generation of inorganic phosphate by spectrophotometry in presence of glutathione	[PMID: 21530277]
Sf9	IC₅₀	4 μM Compound: Benzbromarone	Inhibition of Homo sapiens (human) MRP1 expressed in Sf9 cell membranes assessed as decrease in N-ethyl-maleimide-glutathione-induced inorganic phosphate production by spectrophotometric analysis in presence of glutathione Inhibition of Homo sapiens (human) MRP1 expressed in Sf9 cell membranes assessed as decrease in N-ethyl-maleimide-glutathione-induced inorganic phosphate production by spectrophotometric analysis in presence of glutathione	10.1007/s00044-012-9994-0
Vero	IC₅₀	31.83 μg/mL Compound: Benzbromarone	Cytotoxicity against african green monkey Vero cells measured after 48 hrs by MTT assay Cytotoxicity against african green monkey Vero cells measured after 48 hrs by MTT assay	[PMID: 38547733]

体外実験

Benzbromarone (5-20 μM, 24 h) protects against propofol (HY-B0649)‑induced cytotoxicity in Human brain microvascular endothelial cells (HBMVECs)^[1].
Benzbromarone (5-20 μM, 24 h) mitigates propofol (HY-B0649)‑induced oxidative stress and inhibits expression of pro‑inflammatory cytokines and Chemokines in HBMVECs^[1].
Benzbromarone (1-100 μM, 2-24 h) activats the NRF2 signaling pathway in HepG2 cells^[2].
Benzbromarone (1-30 μM, 24 h) promotes degradation of HSP47 to ameliorate collagen overproduction in human keloid fibroblasts^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:	HBMVECs
Concentration:	5 μM, 10 μM, 20 μM
Incubation Time:	24 h
Result:	Improved the viability reduced by propofol.

Western Blot Analysis^[2]

Cell Line:	HepG2 cells
Concentration:	1 μM, 2 μM, 5 μM, 10 μM, 20 μM, 50 μM, 100 μM
Incubation Time:	2 h, 6 h, 24 h
Result:	Increased NRF2 protein expression in HepG2 cells exposed for 2 h, 6 h and 24 h at any concentration. Significantly accumulated the protein of NRF2 in the nuclear fraction after exposure to 100 μM at any time point. Caused an increase in the protein expression of TRX1 and TRX2. Significantly increased the ratio of oxidized TRX2 to reduced TRX2 at a concentration of 100 μM.

体内実験

Benzbromarone (25-50 mg/kg, Intragastric, once a day for four weeks) aggravates hepatic steatosis in high fat diet (HFD)-induced obese (DIO) mice^[3].
Benzbromarone (10 mg/kg, Oral gavage, once a day for 14 consecutive days) attenuates the nephrotoxicity caused by cisplatin(HY-17394) in cisplatin treated rats^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	High fat diet (HFD)-induced obese (DIO) mice^[3]
Dosage:	25 mg/kg, 50 mg/kg
Administration:	Intragastric (i.g.)
Result:	Aggravated lipid accumulation in the liver of DIO mice. Significantly increased triglyceride accumulation and AST, ALT levels. Regulated multiple lipid metabolism genes and the expression of protein markers associated with apoptosis, endoplasmic reticulum stress, and inflammation in the liver of DIO mice.

Animal Model:	Cisplatin treated rats^[2]
Dosage:	10 mg/kg
Administration:	Oral gavage (p.o.)
Result:	Ameliorated the elevation in serum creatinine and blood urea nitrogen (BUN) levels induced by cisplatin. Counteracted oxidative stress induced by cisplatin and enhances anti-oxidant defenses in kidney. Alleviated the inflammatory events of nephrotoxicity induced by cisplatin. Attenuated cisplatin-induced apoptosis.

臨床実験

分子量

424.08

分子式

C₁₇H₁₂Br₂O₃

CAS 番号

3562-84-3

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC(Br)=C(O)C(Br)=C1)C2=C(CC)OC3=CC=CC=C23

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : ≥ 100 mg/mL (235.80 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3580 mL	11.7902 mL	23.5805 mL
5 mM	0.4716 mL	2.3580 mL	4.7161 mL
10 mM	0.2358 mL	1.1790 mL	2.3580 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (4.90 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 15% Cremophor EL 85% Saline
Solubility: 2.5 mg/mL (5.90 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.91%

Select Batch:

データシート (288 KB) SDS (479 KB)

COA (254 KB) HNMR (260 KB) RP-HPLC (250 KB) LCMS (98 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Huang Z, et al. The protective effects of benzbromarone against propofol-induced inflammation and injury in human brain microvascular endothelial cells (HBMVECs) [J]. Neurotoxicity Research, 2021, 39(5): 1449-1458. [Content Brief]

[2]. Roos N J, et al. The uricosuric benzbromarone disturbs the mitochondrial redox homeostasis and activates the NRF2 signaling pathway in HepG2 cells [J]. Free Radical Biology and Medicine, 2020, 152: 216-226. [Content Brief]

[3]. Park J G, et al. Benzbromarone Induces Targeted Degradation of HSP47 Protein and Improves Hypertrophic Scar Formation [J]. Journal of Investigative Dermatology, 2023. [Content Brief]

[4]. Sun P, et al. Benzbromarone aggravates hepatic steatosis in obese individuals [J]. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 2018, 1864(6): 2067-2077. [Content Brief]

[5]. Abdel-Razek E A N, et al. Benzbromarone mitigates cisplatin nephrotoxicity involving enhanced peroxisome proliferator-activated receptor-alpha (PPAR-α) expression [J]. Life sciences, 2020, 243: 117272. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3580 mL	11.7902 mL	23.5805 mL	58.9511 mL
	5 mM	0.4716 mL	2.3580 mL	4.7161 mL	11.7902 mL
	10 mM	0.2358 mL	1.1790 mL	2.3580 mL	5.8951 mL
	15 mM	0.1572 mL	0.7860 mL	1.5720 mL	3.9301 mL
	20 mM	0.1179 mL	0.5895 mL	1.1790 mL	2.9476 mL
	25 mM	0.0943 mL	0.4716 mL	0.9432 mL	2.3580 mL
	30 mM	0.0786 mL	0.3930 mL	0.7860 mL	1.9650 mL
	40 mM	0.0590 mL	0.2948 mL	0.5895 mL	1.4738 mL
	50 mM	0.0472 mL	0.2358 mL	0.4716 mL	1.1790 mL
	60 mM	0.0393 mL	0.1965 mL	0.3930 mL	0.9825 mL
	80 mM	0.0295 mL	0.1474 mL	0.2948 mL	0.7369 mL
	100 mM	0.0236 mL	0.1179 mL	0.2358 mL	0.5895 mL

Benzbromarone Related Classifications

Metabolic Disease

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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備考

ベンゾブロマロン (Synonyms: Benzbromarone)

カスタマーレビュー

Other Forms of Benzbromarone:

顧客検証

おすすめ

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•関連タンパク質:

Interleukin Related アイソフォーム固有の製品をすべて表示:

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JNK アイソフォーム固有の製品をすべて表示:

HSP アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Benzbromarone Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

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ベンゾブロマロン (Synonyms: Benzbromarone)

カスタマーレビュー

Other Forms of Benzbromarone:

ベンゾブロマロン 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Interleukin Related アイソフォーム固有の製品をすべて表示:

Caspase アイソフォーム固有の製品をすべて表示:

Bcl-2 Family アイソフォーム固有の製品をすべて表示:

NF-κB アイソフォーム固有の製品をすべて表示:

JNK アイソフォーム固有の製品をすべて表示:

HSP アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

ベンゾブロマロン 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Benzbromarone Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

カスタマーレビュー

Request for HNMR Report

Request for HNMR Report

ベンゾブロマロン関連抗体

ベンゾブロマロン関連抗体