Propofol (Synonyms: 2,6-Diisopropylphenol)

Name: Propofol
Brand: MedChemExpress
SKU: HY-B0649
Rating: 5.0 (22 reviews)

Cat. No.: HY-B0649 Purity: 99.82%: Data Sheet
COA

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Propofol potently and directly activates GABA_A receptor and inhibits glutamate receptor mediated excitatory synaptic transmission. Propofol has antinociceptive properties and is used for sedation and hypnotic.

For research use only. We do not sell to patients.

CAS No. : 2078-54-8

Size	Stock	Quantity
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid or liquid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 22 publication(s) in Google Scholar

Other Forms of Propofol:

22 Publications Citing Use of MCE Propofol

Apoptosis Analysis

IHC

: Propofol purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Dec 5;11(49):eadw7972. [Abstract]; Western blot analysis further showed that phosphorylation and protein expression of EphB1 were increased significantly under Propofol (120 mg/kg), Ketamine, or Isoflurane anesthesia.

: Propofol purchased from MedChemExpress. Usage Cited in: Inflammation. 2021 Aug;44(4):1288-1301. [Abstract]; The cell apoptosis of liver tissues of the I/R liver injury mice was detected using TUNEL staining. PRO propofol (40 mg/kg), I/R ischemia/reperfusion, AST aspartate aminotransferase, ALT alanine aminotransferase.

: Propofol purchased from MedChemExpress. Usage Cited in: Inflammation. 2021 Aug;44(4):1288-1301. [Abstract]; PRO (Propofol 40 mg/kg) downregulated the expressions of Bnip3, Cleaved Caspase-3, and Bax, but upregulated the expression of Bcl-2 in the liver tissues of I/R liver injury mice.

: Propofol purchased from MedChemExpress. Usage Cited in: Inflammation. 2021 Aug;44(4):1288-1301. [Abstract]; The expression of Bnip3 of H/R AML12 cells after PRO (Propofol, 6 μg/m.) treatment was determined by immunofluorescence.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

GABA_A^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A549	EC₅₀	9.55 mM Compound: 12; Diprivan(R)	Cytotoxicity against human A549 cells after 24 hrs by MTT assay Cytotoxicity against human A549 cells after 24 hrs by MTT assay	[PMID: 30318437]
GL261	IC₅₀	100 μM Compound: Propofol	Anti-proliferative activity against mouse GL261 cells assessed as cell viability incubated for 2 hrs by CCK8 assay Anti-proliferative activity against mouse GL261 cells assessed as cell viability incubated for 2 hrs by CCK8 assay	[PMID: 37861443]
L929	EC₅₀	4.73 mM Compound: 12; Diprivan(R)	Cytotoxicity against mouse L929 cells after 24 hrs by MTT assay Cytotoxicity against mouse L929 cells after 24 hrs by MTT assay	[PMID: 30318437]
Oocyte	EC₅₀	11.2 μM Compound: Propofol	Effective concentration against GABA-evoked chloride currents mediated by human Gamma-aminobutyric acid GABA-A receptor alpha2-beta2-gamma2L expressed in Xenopus oocytes Effective concentration against GABA-evoked chloride currents mediated by human Gamma-aminobutyric acid GABA-A receptor alpha2-beta2-gamma2L expressed in Xenopus oocytes	[PMID: 15801854]
Oocyte	EC₅₀	44 μM Compound: 1 (Propofol)	Direct activation of chloride current in Xenopus laevis oocytes expressing human alpha-1-beta-1-gamma-2 GABA-A receptor subunits Direct activation of chloride current in Xenopus laevis oocytes expressing human alpha-1-beta-1-gamma-2 GABA-A receptor subunits	[PMID: 9599235]
Oocyte	EC₅₀	5.2 μM Compound: propofol	Agonist activity at human alpha1beta2gamma2L GABAA receptor expressed in Xenopus oocytes assessed as potentiation of GABA-induced current response by two-electrode voltage clamp electrophysiology assay Agonist activity at human alpha1beta2gamma2L GABAA receptor expressed in Xenopus oocytes assessed as potentiation of GABA-induced current response by two-electrode voltage clamp electrophysiology assay	[PMID: 22029276]
Oocyte	EC₅₀	50 μM Compound: propofol	Agonist activity at human alpha1beta2gamma2L GABAA receptor expressed in Xenopus oocytes assessed as potentiation of channel current response by two-electrode voltage clamp electrophysiology assay Agonist activity at human alpha1beta2gamma2L GABAA receptor expressed in Xenopus oocytes assessed as potentiation of channel current response by two-electrode voltage clamp electrophysiology assay	[PMID: 22029276]
Oocyte	EC₅₀	8 μM Compound: 1 (Propofol)	Modulation of GABA-induced chloride currents in Xenopus laevis oocytes expressing human alpha-1-beta-1-gamma-2 GABA-A receptor subunits Modulation of GABA-induced chloride currents in Xenopus laevis oocytes expressing human alpha-1-beta-1-gamma-2 GABA-A receptor subunits	[PMID: 9599235]
Oocyte	IC₅₀	7.3 μM Compound: propofol	Antagonist activity at Torpedo californica nACh receptor expressed in Xenopus oocytes assessed as inhibition of acetylcholine-induced current response Antagonist activity at Torpedo californica nACh receptor expressed in Xenopus oocytes assessed as inhibition of acetylcholine-induced current response	[PMID: 22029276]
U-87MG ATCC	IC₅₀	> 100 μM Compound: Propofol	Anti-proliferative activity against human U-87 MG cells assessed as cell viability incubated for 2 hrs by CCK8 assay Anti-proliferative activity against human U-87 MG cells assessed as cell viability incubated for 2 hrs by CCK8 assay	[PMID: 37861443]

In Vivo

Propofol (i.p.; 40 mg/kg) causes full recovery from sedation occurred 36.3 mins. There is residual antinociception when assessed by ECT but not when assessed by noxious heat^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

178.27

Formula

C₁₂H₁₈O

CAS No.

2078-54-8

Appearance

<18°C Solid,>18°C Liquid

Color

Light yellow to yellow

SMILES

OC1=C(C(C)C)C=CC=C1C(C)C

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (560.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 0.5 mg/mL (2.80 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.6095 mL	28.0473 mL	56.0947 mL
5 mM	1.1219 mL	5.6095 mL	11.2189 mL
10 mM	0.5609 mL	2.8047 mL	5.6095 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (14.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 1 mg/mL (5.61 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Protocol 2

Add each solvent one by one: Saline
Solubility: 2.1 mg/mL (11.78 mM); Clear solution; Need ultrasonic
Protocol 3

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 8.33 mg/mL (46.73 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.82%

Select Batch:

Data Sheet (289 KB) SDS (393 KB)

COA (246 KB) RP-HPLC (260 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. R Nadeson, et al. Antinociceptive Properties of Propofol: Involvement of Spinal Cord Gamma-Aminobutyric acid(A) Receptors. J Pharmacol Exp Ther . 1997 Sep;282(3):1181-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	5.6095 mL	28.0473 mL	56.0947 mL	140.2367 mL
DMSO	5 mM	1.1219 mL	5.6095 mL	11.2189 mL	28.0473 mL
	10 mM	0.5609 mL	2.8047 mL	5.6095 mL	14.0237 mL
	15 mM	0.3740 mL	1.8698 mL	3.7396 mL	9.3491 mL
	20 mM	0.2805 mL	1.4024 mL	2.8047 mL	7.0118 mL
	25 mM	0.2244 mL	1.1219 mL	2.2438 mL	5.6095 mL
	30 mM	0.1870 mL	0.9349 mL	1.8698 mL	4.6746 mL
	40 mM	0.1402 mL	0.7012 mL	1.4024 mL	3.5059 mL
	50 mM	0.1122 mL	0.5609 mL	1.1219 mL	2.8047 mL
	60 mM	0.0935 mL	0.4675 mL	0.9349 mL	2.3373 mL
	80 mM	0.0701 mL	0.3506 mL	0.7012 mL	1.7530 mL
	100 mM	0.0561 mL	0.2805 mL	0.5609 mL	1.4024 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Propofol Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Propofol (Synonyms: 2,6-Diisopropylphenol)

Customer Review

Other Forms of Propofol:

Propofol Related Antibodies

22 Publications Citing Use of MCE Propofol

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Propofol Related Classifications

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