Rapamycin (Synonyms: Sirolimus; AY-22989; NSC 226080)

Name: Rapamycin
Brand: MedChemExpress
SKU: HY-10219
Rating: 5.0 (1338 reviews)

Cat. No.: HY-10219 Pureza: 99.94%: Ficha de datos
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Rapamycin (Sirolimus; AY 22989) is a potent and specific blood-brain barrier-transmissible mTOR inhibitor with an IC₅₀ of 0.1 nM in HEK293 cells. Rapamycin binds to FKBP12 and specifically acts as an allosteric inhibitor of mTORC1. Rapamycin is an autophagy activator, an immunosuppressant.

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No. CAS : 53123-88-9

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10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
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5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
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Revisión del cliente

Based on 1338 publication(s) in Google Scholar

Other Forms of Rapamycin:

Rapamycin (GMP Like) In-stock
Rapamycin-d₃ In-stock
Rapamycin-¹³C,d₃ In-stock
RapaLink-1 In-stock
Rapamycin (GMP) In-stock
Rapamycin (Standard) In-stock
Rapamycin-¹³C,d₃-1 Obtener un presupuesto

1338 Publications Citing Use of MCE Rapamycin

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RT-PCR

: Rapamycin purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Jun 17;9(1):146.; Rapamycin (4 -30 mg/kg; p.o.). Western blot and quantification of P-AKTSer473 and P-S6RP of the skin of PIK3CA^WT and PIK3CA^Tie2-CreER mice treated with either vehicle, rapamycin, miransertib or alpelisib (n = 3–4 per group).

: Rapamycin purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Jun 17;9(1):146.; Rapamycin (4 -30 mg/kg; p.o.). Representative immunofluorescence staining of KI67 in the skin of PIK3CA^WT and PIK3CA^Tie2-CreER mice treated with either vehicle, rapamycin, miransertib or alpelisib. Scale bar: 10 μm.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Nature. 2024 Dec;636(8042):466-473. [Abstract]; Rapamycin (20 nM; 48 h) attenuates GFAP-induced cell death in NG2-3112 cells via inhibition of mTORC1.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Science. 2024 Sep 13;385(6714):eadj1979. [Abstract]; Rapamycin (1 μM; 12 h) significantly suppresses Tra2β-PE skipping 12 hours after T cell activation by inhibiting mTOR signaling.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Immunity. 2024 Mar 12;57(3):513-527.e6. [Abstract]; Rapamycin (50 nM) can inhibit inflammation caused by STAT6 deficiency in macrophages (WT and Stat6^-/- BMDMs).

: Rapamycin purchased from MedChemExpress. Usage Cited in: ACS Nano. 2024 Jan 30;18(4):3733-3751. [Abstract]; The relative protein levels of LC-3BII/Ⅰ(ratios), P62, SOX2, and ROCK1 in Swan 71 cells treated with SC79, Rapamycin (RAPA), or SC79+RAPA.

: Rapamycin purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2023 Jul 5:453:131354.; Rapamycin (50 nM; 24 h) efficiently inhibits the Cobalt-induced hyperphosphorylation of Tau in Ser262 and Thr181, when in H4 cells.

: Rapamycin purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2023 Jul 5:453:131354.; Rapamycin (50 nM; 24 h) partially reverses the cobalt-induced increase in LC3B-II and p62 levels in H4 cells.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Cancer Cell Int. 2023 Apr 16;23(1):68. [Abstract]; Rapamycin (2.5 mM; 24 h) ignificantly reverses the PCK1-sh-induced decreased expression of LC3B-II in SW480-sh cells.

: Rapamycin purchased from MedChemExpress. Usage Cited in: PLoS Pathog. 2023 Mar 27;19(3):e1011295. [Abstract]; Rapamycin (100 nM; 24 h) increases PRRSV replication in Marc-145 cells.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Nature. 2021 Jun;594(7862):271-276. [Abstract]; Rapamycin inhibits the increase in phospho-S6 in Krit1BECKO brain endothelial cells (without affecting the rise in KLF4). f, Immunostaining of hindbrain sections from P6 Krit1iBECKO animals treated with vehicle or Rapamycin for PECAM1 and p-S6. White arrows indicate p-S6 positive endothelial cells in the control but not the Rapmycin treated animal. Yellow arrowheads indicate p-S6 positive neuronal cells. g, Immunostaining of hindbrain sections from P6 Krit1iBECKO animals treated with vehicle

: Rapamycin purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2021 Mar 8;39(3):380-393.e8. [Abstract]; Viability of cells treated for 24 h with bafetinib (5 μM), ibrutinib (5 μM), rapamycin (5 μM) or γ-secretase inhibitor (5 μM). Data were compared using a one-sided paired t test followed by Bonferroni correction.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Jun 12;11(6):454. [Abstract]; Immunofluorescence staining of p62 is conducted in HSC-T6 cells. G-Rg3 pretreatment with 16 μM and Ra are conducted with 100 or 200 nM.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Nature. 2018 Jun;558(7711):540-546. [Abstract]; Western blot and quantification of P-AKT (Ser⁴⁷³) and P-S6RP in the liver, heart and muscle, respectively, of PIK3CA^WT and PIK3CA^CAGG-CreER mice treated with vehicle or Rapamycin directly after Cre induction.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2018 Nov:81:278-292. [Abstract]; Evident LC3 turnover and increased level of Atg5 are found upon Rapamycin treatment, which indicate significant autophagy activation.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Int J Cancer. 2018 Aug 15;143(4):931-943. [Abstract]; H1975 cells are pretreated with Rapamycin (100 nM) for 1 h and then cells are exposed to IFN-γ (100 U/mL). Phosphorylation of AKT, S6, 4E-BP1 are analyzed by western blotting.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Sci Rep. 2018 Mar 7;8(1):4108. [Abstract]; L02 cells exposed to PA (200 μM) with different concentrations of Rapamycin (Rapa) or Chloroquine (CQ) for 24 h. Palmitate (PA) induced higher protein expression of LC3 II/I and p62 compared with control as indicated by western blot.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2018 Feb 1;50(2):144-155. [Abstract]; Raw264.7 macrophages treated without or with Rapamycin (1 μΜ) or Chloroquine (20 μΜ) for 48 h. Western blot shows the protein expression levels of Atg5, Beclin1, LC3, and p62/SQSMT1.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Biotechnol Appl Biochem. 2018 Sep;65(5):665-671. [Abstract]; The mTOR inhibitor Rapamycin augments the autophagy induced by GEM. (A, B) Beclin-1 and LC3B expression is analyzed by western blot after treatment of the cells with GEM (5 μM) and Rapamycin (0, 1, and 2.5 μM) for 48 (A) or 72 (B) h.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Pharmacol Biochem Behav. 2019 Feb:177:1-11. [Abstract]; Effect of treatment with Rapamycin, trehalose, or their combination on autophagy activity measured by quantified immunoreactivity of LC3-II in the s. nigra. MPTP is administered at the dose of 20 mg/kg (i.p., daily) for 4 days to induce PD-like pathology.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Pharmacol Biochem Behav. 2019 Feb:177:1-11. [Abstract]; Effect of treatment with Rapamycin, trehalose, or their combination on tyrosine hydroxylase (TH) expression in the striatum in MPTP-induced mouse model of Parkinson’s disease.

: Rapamycin purchased from MedChemExpress. Usage Cited in: PeerJ. 2018 Nov 21:6:e5988. [Abstract]; C6/36 cells are treated with 3-MA, Rapa or CQ for 36 h, 6 h and 36 h, respectively and then subjected to MDC staining. Mock and DMSO treated C6/36 cells are used as controls.

: Rapamycin purchased from MedChemExpress. Usage Cited in: PeerJ. 2018 Nov 21:6:e5988. [Abstract]; C6/36 cells are treated with 3-MA, Rapa or CQ for 36 h, 6 h and 36 h, respectively. For Rapa plus CQ treatment, C6/36 cells are treated with CQ for 30 h then treated with Rapa for 6 h. Mock and DMSO treated C6/36 cells are used as controls. After the treatment, the levels of AaAtg8-I and AaAtg8-II are analysed by immunoblotting using antibody against AaAtg8.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Cell Physiol Biochem. 2018;48(6):2318-2336. [Abstract]; Cells are pretreated with 5 mM 3-MA or 2 μM for 1 h and then exposed to 40 μM EPA and 10 μM Rapamycin (Rp) for 48 h. Cell extracts are prepared and subjected to western blot analysis.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Cell Physiol Biochem. 2018;48(6):2318-2336. [Abstract]; Cells are treated with Rapamycin (Rp; 10 μM)+Eicosapentaenoic acid (EPA; 40 μM) with or without Chloroquine (CQ; 5 μM) for 48 h. Cell extracts are prepared and subjected to western blot analysis.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Int J Ophthalmol. 2018 May 18;11(5):712-718. [Abstract]; Western blotting shows that Rapamycin treatment downregulates p-mTOR protein levels in infected and uninfected corneal tissues compared to the vehicle group.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Evid Based Complement Alternat Med. 2018 Jun 26:2018:6049498. [Abstract]; Western blot analysis of AKT, p-Akt, mTOR, p-mTOR, FOXO1 and p-FOXO1 expression in U-87 MG cells after treatment with LY294002 (20 μM), Rapamycin (50 nM) or NAC (5 mM) with or without Xihuang pill (XHP).

: Rapamycin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2017 Jun 8;8:15617. [Abstract]; Immunoblot analysis of KRAS protein levels in parental (P) and resistant derivatives (R1 and R2) following 4 h treatment with the corresponding inhibitors Rapamycin, AZD2014, MLN0128, BEZ235 and 4EGI-1. Images are cropped for clarity from the same exposure of the same membrane.

: Rapamycin purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2017 Sep 1;127(9):3339-3352. [Abstract]; Phosphorylation of SMAD1/5/8 and SMAD2/3 in FOP-iMSCs. Serum-starved FOP-iMSCs are pretreated with 10 nM Rapamycin (Rapa), 1 μM DMH1, or 1 μM SB-431542 for 1 hour.

: Rapamycin purchased from MedChemExpress. Usage Cited in: PLoS One. 2017 Jun 21;12(6):e0179772. [Abstract]; RAW264.7 cells transfected with miR-144-3p control or miR-144-3p mimic in full medium with or without 50μg/mL Rapamycin or 0.1% DMSO. 50 μg of total cell extracts are analyzed by Western blotting with a mouse anti-SQSTM1/p62 antibody. The p62 levels are detected by Western-blot (upper). Quantitative analysis of the p62 band normalized to β-actin is shown (lower).

: Rapamycin purchased from MedChemExpress. Usage Cited in: PLoS One. 2017 Nov 29;12(11):e0188748. [Abstract]; FTY720 reverses activation of autophagy induced by mTOR inhibitor, rapamycin. The impact of FTY720 and Rapamycin on the expression of Beclin1 and LC3 is evaluated by western blotting. Results are representative of 4 independent experiments.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2016 Dec 15:122:42-61. [Abstract]; Inhibition of SREBPs processing by AHI is dependent on LKB-1/AMPK/mTOR pathway. (A) HepG2 cells are incubated with or without MHY1485 or Rapamycin for 1 h, the cells are switched to medium D in the presence of vehicle, or AHI. (B) HepG2 cells are incubated with or without Compound C for 1 h, the cells are switched to medium D in the presence of vehicle, or AHI.

: Rapamycin purchased from MedChemExpress. Usage Cited in: Sci Bull. 2015 Dec;60(24):2120-2128.; CNE-2Z cells are treated with AZD8055 or Rapamycin with or without 1 T SMF for 3 d before they are harvested for Western blot.

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Descripciòn

Rapamycin (Sirolimus; AY 22989) is a potent and specific blood-brain barrier-transmissible mTOR inhibitor with an IC₅₀ of 0.1 nM in HEK293 cells. Rapamycin binds to FKBP12 and specifically acts as an allosteric inhibitor of mTORC1^[1]. Rapamycin is an autophagy activator, an immunosuppressant^[2].

IC₅₀ & Target^[1]^[2]

mTOR

0.1 nM (IC₅₀, in HEK293 cells )

Microbial Metabolite

FKBP12

Autophagy

Cellular Effect

Cell Line	Type	Value	Description	References
SUM185PE	GI₅₀	0.04 1 Compound: Rapamycin	Antiproliferative activity against human SUM185PE cells incubated for 48 hrs by sulforhodamine B assay Antiproliferative activity against human SUM185PE cells incubated for 48 hrs by sulforhodamine B assay	[PMID: 32510949]
A549	IC₅₀	18.74 3 Compound: Rapamycin	Antiproliferative activity against human A549 cells by MTT assay Antiproliferative activity against human A549 cells by MTT assay	[PMID: 31546197]
A549	IC₅₀	18.74 3 Compound: Rapamycin	Antiproliferative activity against human A549 cells by MTT assay Antiproliferative activity against human A549 cells by MTT assay	[PMID: 31546197]
MDA-MB-453	GI₅₀	0.05 1 Compound: Rapamycin	Antiproliferative activity against human MDA-MB-453 cells incubated for 48 hrs by sulforhodamine B assay Antiproliferative activity against human MDA-MB-453 cells incubated for 48 hrs by sulforhodamine B assay	[PMID: 32510949]
A549	IC₅₀	30.72 3 Compound: Rapamycin	Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 33862514]
A549	IC₅₀	30.72 3 Compound: Rapamycin	Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 33862514]
MEF	IC₅₀	0.05 1 Compound: Sirolimus	Inhibition of mTOR in mouse TSC1-/- MEF cells assessed as inhibition of S6 Ser240/244 phosphorylation incubated for 2 hrs by fluorescence based assay Inhibition of mTOR in mouse TSC1-/- MEF cells assessed as inhibition of S6 Ser240/244 phosphorylation incubated for 2 hrs by fluorescence based assay	[PMID: 31223435]
A549	IC₅₀	49.35 3 Compound: Rapamycin	Antitumor activity against human A549 cells assessed as cell growth inhibition incubated for 72 hrs by Cell Titer-Glo assay Antitumor activity against human A549 cells assessed as cell growth inhibition incubated for 72 hrs by Cell Titer-Glo assay	[PMID: 35688004]
A549	IC₅₀	49.35 3 Compound: Rapamycin	Antitumor activity against human A549 cells assessed as cell growth inhibition incubated for 72 hrs by Cell Titer-Glo assay Antitumor activity against human A549 cells assessed as cell growth inhibition incubated for 72 hrs by Cell Titer-Glo assay	[PMID: 35688004]
C-33-A	IC₅₀	< 50 1 Compound: 25	Antiproliferative activity against human C-33-A cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay Antiproliferative activity against human C-33-A cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay	[PMID: 36669398]
HEK293	EC₅₀	0.05 3 Compound: Rapamycin	Inhibition of TPA-induced degradation of Pdcd4 (amino acid 39-91) expressed in human HEK293 cells assessed as minimum compound concentration required for 50% recovery of Pdcd4-luciferase signal incubated for 8 hrs by luciferase reporter gene assay Inhibition of TPA-induced degradation of Pdcd4 (amino acid 39-91) expressed in human HEK293 cells assessed as minimum compound concentration required for 50% recovery of Pdcd4-luciferase signal incubated for 8 hrs by luciferase reporter gene assay	[PMID: 21539301]
HEK293	IC₅₀	0.1 1 Compound: Rapamycin	Inhibition of mTOR in HEK293 cells Inhibition of mTOR in HEK293 cells	[PMID: 32324396]
HEK293	IC₅₀	0.1 1 Compound: rapamycin	Inhibition of mTOR kinase expressed in human HEK293 cells by Western blot analysis Inhibition of mTOR kinase expressed in human HEK293 cells by Western blot analysis	[PMID: 17350953]
HEK293	IC₅₀	0.1 1 Compound: rapamycin	Inhibition of mTOR kinase expressed in human HEK293 cells by Western blot analysis Inhibition of mTOR kinase expressed in human HEK293 cells by Western blot analysis	[PMID: 17350953]
HEK-293T	EC₅₀	0.1 1 Compound: RAPA	Antiviral activity against HIV1 R5 assessed as inhibition of cell-cell fusion between R5-envelope expressing HEK293T cells and CD8 depleted human PBMCs pretreated for 6 days Antiviral activity against HIV1 R5 assessed as inhibition of cell-cell fusion between R5-envelope expressing HEK293T cells and CD8 depleted human PBMCs pretreated for 6 days	[PMID: 17485501]
DU-145	IC₅₀	4 3 Compound: Rapamycin	Antitumor activity against human DU-145 cells assessed as cell growth inhibition Antitumor activity against human DU-145 cells assessed as cell growth inhibition	[PMID: 35688004]
HEK-293T	EC₅₀	0.1 1 Compound: RAPA	Antiviral activity against HIV1 R5 assessed as inhibition of cell-cell fusion between R5-envelope expressing HEK293T cells and CD8 depleted human PBMCs pretreated for 6 days Antiviral activity against HIV1 R5 assessed as inhibition of cell-cell fusion between R5-envelope expressing HEK293T cells and CD8 depleted human PBMCs pretreated for 6 days	[PMID: 17485501]
HEK-293T	EC₅₀	1.3 1 Compound: RAPA	Antiviral activity against HIV1 X4 assessed as inhibition of cell-cell fusion between X4-envelope expressing HEK293T cells and CD8 depleted human PBMCs pretreated for 6 days Antiviral activity against HIV1 X4 assessed as inhibition of cell-cell fusion between X4-envelope expressing HEK293T cells and CD8 depleted human PBMCs pretreated for 6 days	[PMID: 17485501]
A549	IC₅₀	30.72 3 Compound: Rapamycin	Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 33862514]
A549	IC₅₀	18.74 3 Compound: Rapamycin	Antiproliferative activity against human A549 cells by MTT assay Antiproliferative activity against human A549 cells by MTT assay	[PMID: 31546197]
HeLa	IC₅₀	> 10000 1 Compound: Rapamycin	Growth inhibition of human HeLa cells after 72 hrs by CCK8 assay Growth inhibition of human HeLa cells after 72 hrs by CCK8 assay	[PMID: 29308895]
A549	IC₅₀	49.35 3 Compound: Rapamycin	Antitumor activity against human A549 cells assessed as cell growth inhibition incubated for 72 hrs by Cell Titer-Glo assay Antitumor activity against human A549 cells assessed as cell growth inhibition incubated for 72 hrs by Cell Titer-Glo assay	[PMID: 35688004]
HepG2	EC₅₀	10 3 Compound: Sirolimus	Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis	[PMID: 20966043]
HepG2	CC₅₀	67.2 3 Compound: 78	Cytotoxicity against human HepG2 assessed as reduction in cell viability after 24 hrs by CellTiter-Glo luminescent assay Cytotoxicity against human HepG2 assessed as reduction in cell viability after 24 hrs by CellTiter-Glo luminescent assay	[PMID: 28051303]
HEK-293T	EC₅₀	1.3 1 Compound: RAPA	Antiviral activity against HIV1 X4 assessed as inhibition of cell-cell fusion between X4-envelope expressing HEK293T cells and CD8 depleted human PBMCs pretreated for 6 days Antiviral activity against HIV1 X4 assessed as inhibition of cell-cell fusion between X4-envelope expressing HEK293T cells and CD8 depleted human PBMCs pretreated for 6 days	[PMID: 17485501]
T-cell	IC₅₀	1.6 1 Compound: 1	T-cell antiproliferative activity in human mixed lymphocyte reaction T-cell antiproliferative activity in human mixed lymphocyte reaction	[PMID: 16185865]
MCF7	EC₅₀	< 1 1 Compound: Rapamycin	Cytotoxicity against human MCF7 cells after 5 days by Presto blue reagent-based fluorescence analysis Cytotoxicity against human MCF7 cells after 5 days by Presto blue reagent-based fluorescence analysis	10.1039/C5MD00493D
MCF7	IC₅₀	18.74 3 Compound: Rapamycin	Antiproliferative activity against human MCF7 cells by MTT assay Antiproliferative activity against human MCF7 cells by MTT assay	[PMID: 31546197]
DU-145	IC₅₀	4 3 Compound: Rapamycin	Antitumor activity against human DU-145 cells assessed as cell growth inhibition Antitumor activity against human DU-145 cells assessed as cell growth inhibition	[PMID: 35688004]
HCT-8	IC₅₀	1.25 3 Compound: Sirolimus	TP_TRANSPORTER: inhibition of Rhodamine 123 transport (basal to apical) (R123: 15 uM) in HCT-8 cells TP_TRANSPORTER: inhibition of Rhodamine 123 transport (basal to apical) (R123: 15 uM) in HCT-8 cells	[PMID: 12235265]
MCF7	IC₅₀	4980 1 Compound: Rapamycin	Growth inhibition of human MCF7 cells after 72 hrs by CCK8 assay Growth inhibition of human MCF7 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
HEK293	EC₅₀	0.05 3 Compound: Rapamycin	Inhibition of TPA-induced degradation of Pdcd4 (amino acid 39-91) expressed in human HEK293 cells assessed as minimum compound concentration required for 50% recovery of Pdcd4-luciferase signal incubated for 8 hrs by luciferase reporter gene assay Inhibition of TPA-induced degradation of Pdcd4 (amino acid 39-91) expressed in human HEK293 cells assessed as minimum compound concentration required for 50% recovery of Pdcd4-luciferase signal incubated for 8 hrs by luciferase reporter gene assay	[PMID: 21539301]
MDA-MB-231	GI₅₀	> 100 1 Compound: Rapamycin	Antiproliferative activity against human MDA-MB-231 cells incubated for 48 hrs by sulforhodamine B assay Antiproliferative activity against human MDA-MB-231 cells incubated for 48 hrs by sulforhodamine B assay	[PMID: 32510949]
T-cell	IC₅₀	9.8 1 Compound: 1	Tested for inhibition of T cell proliferation in the co-mitogen stimulated proliferation assay Tested for inhibition of T cell proliferation in the co-mitogen stimulated proliferation assay	10.1016/0960-894X(95)00223-G
MDA-MB-231	IC₅₀	> 10000 1 Compound: Rapamycin	Growth inhibition of human MDA-MB-231 cells after 72 hrs by CCK8 assay Growth inhibition of human MDA-MB-231 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
HEK293	IC₅₀	1.1 3 Compound: Rapamycin	TP_TRANSPORTER: inhibition of Phalloidin uptake (Phalloidin: 1 uM) in OATP-C-expressing HEK293 cells TP_TRANSPORTER: inhibition of Phalloidin uptake (Phalloidin: 1 uM) in OATP-C-expressing HEK293 cells	[PMID: 14530907]
MDA-MB-231	IC₅₀	0.9 3 Compound: Rapamycin	Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 24 hrs Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 24 hrs	[PMID: 33139111]
HeLa	IC₅₀	0.77 3 Compound: Rapamycin	Cytotoxicity against human HeLa cells Cytotoxicity against human HeLa cells	[PMID: 30015070]
MDA-MB-231	IC₅₀	35.79 3 Compound: Rapamycin	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 33862514]
HeLa	IC₅₀	>10 3 Compound: Rapamycin	Growth inhibition of human HeLa cells after 72 hrs by CCK8 assay Growth inhibition of human HeLa cells after 72 hrs by CCK8 assay	[PMID: 29308895]
MDA-MB-453	GI₅₀	0.05 1 Compound: Rapamycin	Antiproliferative activity against human MDA-MB-453 cells incubated for 48 hrs by sulforhodamine B assay Antiproliferative activity against human MDA-MB-453 cells incubated for 48 hrs by sulforhodamine B assay	[PMID: 32510949]
HepG2	CC₅₀	67.2 3 Compound: 78	Cytotoxicity against human HepG2 assessed as reduction in cell viability after 24 hrs by CellTiter-Glo luminescent assay Cytotoxicity against human HepG2 assessed as reduction in cell viability after 24 hrs by CellTiter-Glo luminescent assay	[PMID: 28051303]
HCT-8	IC₅₀	1.25 3 Compound: Sirolimus	TP_TRANSPORTER: inhibition of Rhodamine 123 transport (basal to apical) (R123: 15 uM) in HCT-8 cells TP_TRANSPORTER: inhibition of Rhodamine 123 transport (basal to apical) (R123: 15 uM) in HCT-8 cells	[PMID: 12235265]
MDA-MB-468	IC₅₀	37.2 3 Compound: Rapamycin	Antiproliferative activity against human MDA-MB-468 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Antiproliferative activity against human MDA-MB-468 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 33862514]
HEK-293T	EC₅₀	0.1 1 Compound: RAPA	Antiviral activity against HIV1 R5 assessed as inhibition of cell-cell fusion between R5-envelope expressing HEK293T cells and CD8 depleted human PBMCs pretreated for 6 days Antiviral activity against HIV1 R5 assessed as inhibition of cell-cell fusion between R5-envelope expressing HEK293T cells and CD8 depleted human PBMCs pretreated for 6 days	[PMID: 17485501]
MEF	IC₅₀	0.05 1 Compound: Sirolimus	Inhibition of mTOR in mouse TSC1-/- MEF cells assessed as inhibition of S6 Ser240/244 phosphorylation incubated for 2 hrs by fluorescence based assay Inhibition of mTOR in mouse TSC1-/- MEF cells assessed as inhibition of S6 Ser240/244 phosphorylation incubated for 2 hrs by fluorescence based assay	[PMID: 31223435]
HepG2	EC₅₀	10 3 Compound: Sirolimus	Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis	[PMID: 20966043]
HEK-293T	EC₅₀	1.3 1 Compound: RAPA	Antiviral activity against HIV1 X4 assessed as inhibition of cell-cell fusion between X4-envelope expressing HEK293T cells and CD8 depleted human PBMCs pretreated for 6 days Antiviral activity against HIV1 X4 assessed as inhibition of cell-cell fusion between X4-envelope expressing HEK293T cells and CD8 depleted human PBMCs pretreated for 6 days	[PMID: 17485501]
MV4-11	IC₅₀	> 500 1 Compound: RAPA	Antiproliferative activity against human MV4-11 cells after 72 hrs by MTT assay Antiproliferative activity against human MV4-11 cells after 72 hrs by MTT assay	[PMID: 30629434]
K562	IC₅₀	1 2 Compound: rapamycin	Antiproliferative activity against human K562 cells Antiproliferative activity against human K562 cells	[PMID: 18571290]
HEK293	EC₅₀	0.05 3 Compound: Rapamycin	Inhibition of TPA-induced degradation of Pdcd4 (amino acid 39-91) expressed in human HEK293 cells assessed as minimum compound concentration required for 50% recovery of Pdcd4-luciferase signal incubated for 8 hrs by luciferase reporter gene assay Inhibition of TPA-induced degradation of Pdcd4 (amino acid 39-91) expressed in human HEK293 cells assessed as minimum compound concentration required for 50% recovery of Pdcd4-luciferase signal incubated for 8 hrs by luciferase reporter gene assay	[PMID: 21539301]
NCI-H460	IC₅₀	> 10000 1 Compound: Rapamycin	Growth inhibition of human H460 cells after 72 hrs by CCK8 assay Growth inhibition of human H460 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
MCF7	IC₅₀	18.74 3 Compound: Rapamycin	Antiproliferative activity against human MCF7 cells by MTT assay Antiproliferative activity against human MCF7 cells by MTT assay	[PMID: 31546197]
MDA-MB-231	IC₅₀	0.9 3 Compound: Rapamycin	Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 24 hrs Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 24 hrs	[PMID: 33139111]
HEK293	IC₅₀	0.1 1 Compound: Rapamycin	Inhibition of mTOR in HEK293 cells Inhibition of mTOR in HEK293 cells	[PMID: 32324396]
OCI-AML2	IC₅₀	> 500 1 Compound: RAPA	Antiproliferative activity against human OCI-AML2 cells after 72 hrs by MTT assay Antiproliferative activity against human OCI-AML2 cells after 72 hrs by MTT assay	[PMID: 30629434]
MDA-MB-231	IC₅₀	35.79 3 Compound: Rapamycin	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 33862514]
HEK293	IC₅₀	0.1 1 Compound: rapamycin	Inhibition of mTOR kinase expressed in human HEK293 cells by Western blot analysis Inhibition of mTOR kinase expressed in human HEK293 cells by Western blot analysis	[PMID: 17350953]
OCI-AML-3	IC₅₀	> 500 1 Compound: RAPA	Antiproliferative activity against human OCI-AML3 cells after 72 hrs by MTT assay Antiproliferative activity against human OCI-AML3 cells after 72 hrs by MTT assay	[PMID: 30629434]
HEK293	IC₅₀	1.1 3 Compound: Rapamycin	TP_TRANSPORTER: inhibition of Phalloidin uptake (Phalloidin: 1 uM) in OATP-C-expressing HEK293 cells TP_TRANSPORTER: inhibition of Phalloidin uptake (Phalloidin: 1 uM) in OATP-C-expressing HEK293 cells	[PMID: 14530907]
OVCAR-5	IC₅₀	> 10000 1 Compound: Rapamycin	Growth inhibition of human OVCAR5 cells after 72 hrs by CCK8 assay Growth inhibition of human OVCAR5 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
MDA-MB-231	IC₅₀	>10 3 Compound: Rapamycin	Growth inhibition of human MDA-MB-231 cells after 72 hrs by CCK8 assay Growth inhibition of human MDA-MB-231 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
PC-3	EC₅₀	43.1 3 Compound: Rapamycin	Cytotoxicity against human PC3 cells by MTT assay Cytotoxicity against human PC3 cells by MTT assay	[PMID: 28774426]
MDA-MB-468	IC₅₀	37.2 3 Compound: Rapamycin	Antiproliferative activity against human MDA-MB-468 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Antiproliferative activity against human MDA-MB-468 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 33862514]
NCI-H460	IC₅₀	>10 3 Compound: Rapamycin	Growth inhibition of human H460 cells after 72 hrs by CCK8 assay Growth inhibition of human H460 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
SiHa	IC₅₀	1756 1 Compound: Rapamycin	Growth inhibition of human SiHa cells after 72 hrs by CCK8 assay Growth inhibition of human SiHa cells after 72 hrs by CCK8 assay	[PMID: 29308895]
HT-3	IC₅₀	< 50 1 Compound: 25	Antiproliferative activity against human HT-3 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay Antiproliferative activity against human HT-3 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay	[PMID: 36669398]
SK-OV-3	IC₅₀	> 10000 1 Compound: Rapamycin	Growth inhibition of human SKOV3 cells after 72 hrs by CCK8 assay Growth inhibition of human SKOV3 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
OVCAR-5	IC₅₀	>10 3 Compound: Rapamycin	Growth inhibition of human OVCAR5 cells after 72 hrs by CCK8 assay Growth inhibition of human OVCAR5 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
PC-3	EC₅₀	43.1 3 Compound: Rapamycin	Cytotoxicity against human PC3 cells by MTT assay Cytotoxicity against human PC3 cells by MTT assay	[PMID: 28774426]
HeLa	IC₅₀	0.77 3 Compound: Rapamycin	Cytotoxicity against human HeLa cells Cytotoxicity against human HeLa cells	[PMID: 30015070]
SUM185PE	GI₅₀	0.04 1 Compound: Rapamycin	Antiproliferative activity against human SUM185PE cells incubated for 48 hrs by sulforhodamine B assay Antiproliferative activity against human SUM185PE cells incubated for 48 hrs by sulforhodamine B assay	[PMID: 32510949]
HeLa	IC₅₀	> 10000 1 Compound: Rapamycin	Growth inhibition of human HeLa cells after 72 hrs by CCK8 assay Growth inhibition of human HeLa cells after 72 hrs by CCK8 assay	[PMID: 29308895]
T47D	IC₅₀	1576 1 Compound: Rapamycin	Growth inhibition of human T47D cells after 72 hrs by CCK8 assay Growth inhibition of human T47D cells after 72 hrs by CCK8 assay	[PMID: 29308895]
PC-3	IC₅₀	<0.01 3 Compound: Rapamycin	Antiproliferative activity against human PC3 cells Antiproliferative activity against human PC3 cells	[PMID: 21978683]
HepG2	CC₅₀	67.2 3 Compound: 78	Cytotoxicity against human HepG2 assessed as reduction in cell viability after 24 hrs by CellTiter-Glo luminescent assay Cytotoxicity against human HepG2 assessed as reduction in cell viability after 24 hrs by CellTiter-Glo luminescent assay	[PMID: 28051303]
PC-3	IC₅₀	1.3 3 Compound: Rapamycin	Antitumor activity against human PC-3 cells assessed as cell growth inhibition Antitumor activity against human PC-3 cells assessed as cell growth inhibition	[PMID: 35688004]
PC-3	IC₅₀	>10 3 Compound: Rapamycin	Inhibition of mTOR-mediated Akt phosphorylation in human PC3 cells Inhibition of mTOR-mediated Akt phosphorylation in human PC3 cells	[PMID: 21978683]
HepG2	EC₅₀	10 3 Compound: Sirolimus	Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis	[PMID: 20966043]
SF-268	IC₅₀	2.7 3 Compound: Rapamycin	Antitumor activity against human SF-268 cells assessed as cell growth inhibition Antitumor activity against human SF-268 cells assessed as cell growth inhibition	[PMID: 35688004]
T47D	IC₅₀	1576 1 Compound: Rapamycin	Growth inhibition of human T47D cells after 72 hrs by CCK8 assay Growth inhibition of human T47D cells after 72 hrs by CCK8 assay	[PMID: 29308895]
SiHa	IC₅₀	1756 1 Compound: Rapamycin	Growth inhibition of human SiHa cells after 72 hrs by CCK8 assay Growth inhibition of human SiHa cells after 72 hrs by CCK8 assay	[PMID: 29308895]
SK-MEL-2	IC₅₀	1 3 Compound: Rapamycin	Synergistic cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition measured for 48 hrs in presence of LY3214996 by MTT assay Synergistic cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition measured for 48 hrs in presence of LY3214996 by MTT assay	[PMID: 36961300]
K562	IC₅₀	1 2 Compound: rapamycin	Antiproliferative activity against human K562 cells Antiproliferative activity against human K562 cells	[PMID: 18571290]
T-cell	IC₅₀	2600 1 Compound: 1	Antiproliferative activity in Lewis rat lymph node cells in rat mixed lymphocyte reaction Antiproliferative activity in Lewis rat lymph node cells in rat mixed lymphocyte reaction	[PMID: 16185865]
SK-OV-3	IC₅₀	>10 3 Compound: Rapamycin	Growth inhibition of human SKOV3 cells after 72 hrs by CCK8 assay Growth inhibition of human SKOV3 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
MCF7	IC₅₀	4980 1 Compound: Rapamycin	Growth inhibition of human MCF7 cells after 72 hrs by CCK8 assay Growth inhibition of human MCF7 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
MCF7	EC₅₀	< 1 1 Compound: Rapamycin	Cytotoxicity against human MCF7 cells after 5 days by Presto blue reagent-based fluorescence analysis Cytotoxicity against human MCF7 cells after 5 days by Presto blue reagent-based fluorescence analysis	10.1039/C5MD00493D
MCF7	EC₅₀	<1 1 Compound: Rapamycin	Cytotoxicity against human MCF7 cells after 5 days by Presto blue reagent-based fluorescence analysis Cytotoxicity against human MCF7 cells after 5 days by Presto blue reagent-based fluorescence analysis	10.1039/C5MD00493D
MCF7	IC₅₀	18.74 3 Compound: Rapamycin	Antiproliferative activity against human MCF7 cells by MTT assay Antiproliferative activity against human MCF7 cells by MTT assay	[PMID: 31546197]
U-87MG ATCC	IC₅₀	10 3 Compound: Rapamycin	Antitumor activity against human U-87 MG cells assessed as cell growth inhibition Antitumor activity against human U-87 MG cells assessed as cell growth inhibition	[PMID: 35688004]
MCF7	IC₅₀	4980 1 Compound: Rapamycin	Growth inhibition of human MCF7 cells after 72 hrs by CCK8 assay Growth inhibition of human MCF7 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
C-33-A	IC₅₀	<50 1 Compound: 25	Antiproliferative activity against human C-33-A cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay Antiproliferative activity against human C-33-A cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay	[PMID: 36669398]
Caov-3 cell line	IC₅₀	<50 1 Compound: 25	Antiproliferative activity against human Caov-3 cell line assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay Antiproliferative activity against human Caov-3 cell line assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay	[PMID: 36669398]
MDA-MB-231	GI₅₀	> 100 1 Compound: Rapamycin	Antiproliferative activity against human MDA-MB-231 cells incubated for 48 hrs by sulforhodamine B assay Antiproliferative activity against human MDA-MB-231 cells incubated for 48 hrs by sulforhodamine B assay	[PMID: 32510949]
HT-3	IC₅₀	<50 1 Compound: 25	Antiproliferative activity against human HT-3 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay Antiproliferative activity against human HT-3 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay	[PMID: 36669398]
MDA-MB-231	IC₅₀	0.9 3 Compound: Rapamycin	Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 24 hrs Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 24 hrs	[PMID: 33139111]
ME-180	IC₅₀	<50 1 Compound: 25	Antiproliferative activity against human ME-180 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay Antiproliferative activity against human ME-180 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay	[PMID: 36669398]
MDA-MB-231	IC₅₀	35.79 3 Compound: Rapamycin	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 33862514]
OVCAR-8	IC₅₀	<50 1 Compound: 25	Antiproliferative activity against human OVCAR-8 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay Antiproliferative activity against human OVCAR-8 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay	[PMID: 36669398]
SK-OV-3	IC₅₀	<50 1 Compound: 25	Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay	[PMID: 36669398]
MDA-MB-231	IC₅₀	> 10000 1 Compound: Rapamycin	Growth inhibition of human MDA-MB-231 cells after 72 hrs by CCK8 assay Growth inhibition of human MDA-MB-231 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
MDA-MB-231	GI₅₀	>100 1 Compound: Rapamycin	Antiproliferative activity against human MDA-MB-231 cells incubated for 48 hrs by sulforhodamine B assay Antiproliferative activity against human MDA-MB-231 cells incubated for 48 hrs by sulforhodamine B assay	[PMID: 32510949]
MV4-11	IC₅₀	>500 1 Compound: RAPA	Antiproliferative activity against human MV4-11 cells after 72 hrs by MTT assay Antiproliferative activity against human MV4-11 cells after 72 hrs by MTT assay	[PMID: 30629434]
MDA-MB-453	GI₅₀	0.05 1 Compound: Rapamycin	Antiproliferative activity against human MDA-MB-453 cells incubated for 48 hrs by sulforhodamine B assay Antiproliferative activity against human MDA-MB-453 cells incubated for 48 hrs by sulforhodamine B assay	[PMID: 32510949]
MDA-MB-468	IC₅₀	37.2 3 Compound: Rapamycin	Antiproliferative activity against human MDA-MB-468 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Antiproliferative activity against human MDA-MB-468 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 33862514]
OCI-AML2	IC₅₀	>500 1 Compound: RAPA	Antiproliferative activity against human OCI-AML2 cells after 72 hrs by MTT assay Antiproliferative activity against human OCI-AML2 cells after 72 hrs by MTT assay	[PMID: 30629434]
ME-180	IC₅₀	< 50 1 Compound: 25	Antiproliferative activity against human ME-180 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay Antiproliferative activity against human ME-180 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay	[PMID: 36669398]
OCI-AML-3	IC₅₀	>500 1 Compound: RAPA	Antiproliferative activity against human OCI-AML3 cells after 72 hrs by MTT assay Antiproliferative activity against human OCI-AML3 cells after 72 hrs by MTT assay	[PMID: 30629434]
MEF	IC₅₀	0.05 1 Compound: Sirolimus	Inhibition of mTOR in mouse TSC1-/- MEF cells assessed as inhibition of S6 Ser240/244 phosphorylation incubated for 2 hrs by fluorescence based assay Inhibition of mTOR in mouse TSC1-/- MEF cells assessed as inhibition of S6 Ser240/244 phosphorylation incubated for 2 hrs by fluorescence based assay	[PMID: 31223435]
MV4-11	IC₅₀	> 500 1 Compound: RAPA	Antiproliferative activity against human MV4-11 cells after 72 hrs by MTT assay Antiproliferative activity against human MV4-11 cells after 72 hrs by MTT assay	[PMID: 30629434]
NCI-H460	IC₅₀	> 10000 1 Compound: Rapamycin	Growth inhibition of human H460 cells after 72 hrs by CCK8 assay Growth inhibition of human H460 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
OCI-AML-3	IC₅₀	> 500 1 Compound: RAPA	Antiproliferative activity against human OCI-AML3 cells after 72 hrs by MTT assay Antiproliferative activity against human OCI-AML3 cells after 72 hrs by MTT assay	[PMID: 30629434]
OCI-AML2	IC₅₀	> 500 1 Compound: RAPA	Antiproliferative activity against human OCI-AML2 cells after 72 hrs by MTT assay Antiproliferative activity against human OCI-AML2 cells after 72 hrs by MTT assay	[PMID: 30629434]
OVCAR-5	IC₅₀	> 10000 1 Compound: Rapamycin	Growth inhibition of human OVCAR5 cells after 72 hrs by CCK8 assay Growth inhibition of human OVCAR5 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
OVCAR-8	IC₅₀	< 50 1 Compound: 25	Antiproliferative activity against human OVCAR-8 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay Antiproliferative activity against human OVCAR-8 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay	[PMID: 36669398]
PC-3	EC₅₀	43.1 3 Compound: Rapamycin	Cytotoxicity against human PC3 cells by MTT assay Cytotoxicity against human PC3 cells by MTT assay	[PMID: 28774426]
PC-3	IC₅₀	1.3 3 Compound: Rapamycin	Antitumor activity against human PC-3 cells assessed as cell growth inhibition Antitumor activity against human PC-3 cells assessed as cell growth inhibition	[PMID: 35688004]
PC-3	IC₅₀	< 0.01 3 Compound: Rapamycin	Antiproliferative activity against human PC3 cells Antiproliferative activity against human PC3 cells	[PMID: 21978683]
PC-3	IC₅₀	< 0.01 3 Compound: Rapamycin	Inhibition of mTOR-mediated S6 phosphorylation in human PC3 cells Inhibition of mTOR-mediated S6 phosphorylation in human PC3 cells	[PMID: 21978683]
PC-3	IC₅₀	> 10 3 Compound: Rapamycin	Inhibition of mTOR-mediated Akt phosphorylation in human PC3 cells Inhibition of mTOR-mediated Akt phosphorylation in human PC3 cells	[PMID: 21978683]
SF-268	IC₅₀	2.7 3 Compound: Rapamycin	Antitumor activity against human SF-268 cells assessed as cell growth inhibition Antitumor activity against human SF-268 cells assessed as cell growth inhibition	[PMID: 35688004]
SK-MEL-2	IC₅₀	1 3 Compound: Rapamycin	Synergistic cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition measured for 48 hrs in presence of LY3214996 by MTT assay Synergistic cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition measured for 48 hrs in presence of LY3214996 by MTT assay	[PMID: 36961300]
SK-MEL-2	IC₅₀	1 3 Compound: Rapamycin	Synergistic cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition measured for 48 hrs in presence of MEK-162 by MTT assay Synergistic cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition measured for 48 hrs in presence of MEK-162 by MTT assay	[PMID: 36961300]
SK-OV-3	IC₅₀	< 50 1 Compound: 25	Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay	[PMID: 36669398]
SK-OV-3	IC₅₀	> 10000 1 Compound: Rapamycin	Growth inhibition of human SKOV3 cells after 72 hrs by CCK8 assay Growth inhibition of human SKOV3 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
SUM185PE	GI₅₀	0.04 1 Compound: Rapamycin	Antiproliferative activity against human SUM185PE cells incubated for 48 hrs by sulforhodamine B assay Antiproliferative activity against human SUM185PE cells incubated for 48 hrs by sulforhodamine B assay	[PMID: 32510949]
SiHa	IC₅₀	1756 1 Compound: Rapamycin	Growth inhibition of human SiHa cells after 72 hrs by CCK8 assay Growth inhibition of human SiHa cells after 72 hrs by CCK8 assay	[PMID: 29308895]
T-cell	IC₅₀	1.6 1 Compound: 1	T-cell antiproliferative activity in human mixed lymphocyte reaction T-cell antiproliferative activity in human mixed lymphocyte reaction	[PMID: 16185865]
T-cell	IC₅₀	2600 1 Compound: 1	Antiproliferative activity in Lewis rat lymph node cells in rat mixed lymphocyte reaction Antiproliferative activity in Lewis rat lymph node cells in rat mixed lymphocyte reaction	[PMID: 16185865]
T-cell	IC₅₀	9.8 1 Compound: 1	Tested for inhibition of T cell proliferation in the co-mitogen stimulated proliferation assay Tested for inhibition of T cell proliferation in the co-mitogen stimulated proliferation assay	10.1016/0960-894X(95)00223-G
T47D	IC₅₀	1576 1 Compound: Rapamycin	Growth inhibition of human T47D cells after 72 hrs by CCK8 assay Growth inhibition of human T47D cells after 72 hrs by CCK8 assay	[PMID: 29308895]
U-87MG ATCC	IC₅₀	10 3 Compound: Rapamycin	Antitumor activity against human U-87 MG cells assessed as cell growth inhibition Antitumor activity against human U-87 MG cells assessed as cell growth inhibition	[PMID: 35688004]

In Vitro

Rapamycin (12.5-100 nM; 24 hours) treatment exerts modest inhibitory effect on lung cancer cell proliferation in a dose-dependent manner in all cell lines (A549, SPC-A-1, 95D and NCI-H446 cells) tested, achieving about 30-40% reduction in cell proliferation at 100 nM vs. ~10% reduction at 12.5 nM^[3].
Lung cancer cell line 95D cells are exposed to Rapamycin (10 nM, 20 nM) and RP-56976 (1 nM, 10 nM) alone or in combination (Rapamycin 20 nM+ RP-56976 10 nM). After 24 hours exposure to Rapamycin or RP-56976 alone does not significantly alter the level of expression or phosphorylation of ERK1/2, whereas cells treated with the combination of Rapamycin with RP-56976 exhibit a marked reduction in the phosphorylation levels of ERK1/2^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	Lung cancer cell lines A549, SPC-A-1, 95D and NCI-H446
Concentration:	12.5 nM, 25 nM, 50 nM, 100 nM
Incubation Time:	24 hours
Result:	Treatment exerted modest inhibitory effect on lung cancer cell proliferation in a dose-dependent manner in all cell lines.

Western Blot Analysis^[3]

Cell Line:	95D cells
Concentration:	10 nM and 20 nM
Incubation Time:	24 hours
Result:	Combination treatment with RP-56976 decreased phosphorylation of ERK.

In Vivo

Rapamycin (2.0 mg/kg; intraperitoneal injection; every other day; 28 days) alone has a moderate inhibitory effect. However, the combination of Metformin (HY-B0627) and Rapamycin exerts a significantly increased inhibition of tumor growth compared with the control group, the Rapamycin monotherapy group and the Metformin monotherapy group^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	24 male nu/nu mice aged 4-5 week old (15-20 g)^[4]
Dosage:	2.0 mg/kg
Administration:	Intraperitoneal injection; every other day; 28 days
Result:	Had a moderate inhibitory effect in monotherapy group. The combination with Metformin exerted a significantly increased inhibition of tumor growth.

Ensayo clínico

Peso molecular

914.17

Fòrmula

C₅₁H₇₉NO₁₃

No. CAS

53123-88-9

Appearance

Solid

Color

White to off-white

SMILES

O=C([C@@]1(O)[C@@H](CC[C@@H](C[C@@H](/C(C)=C/C=C/C=C/[C@H](C[C@@H](C)C([C@@H]([C@@H](/C(C)=C/[C@H]2C)O)OC)=O)C)OC)O1)C)C(N3CCCC[C@H]3C(O[C@@H](CC2=O)[C@@H](C[C@H]4C[C@H]([C@H](O)CC4)OC)C)=O)=O

Structure Classification

Initial Source

Microorganisms

bacterium Streptomyces hygroscopicus

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (109.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.0939 mL	5.4694 mL	10.9389 mL
5 mM	0.2188 mL	1.0939 mL	2.1878 mL
10 mM	0.1094 mL	0.5469 mL	1.0939 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (2.73 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 99.94%

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Ficha de datos (286 KB) SDS (396 KB)

COA (259 KB) HNMR (241 KB) LCMS (121 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Edwards SR, et al. The rapamycin-binding domain of the protein kinase mammalian target of rapamycin is a destabilizing domain. J Biol Chem, 2007, 282(18), 13395-13401. [Content Brief]

[2]. Rangaraju S, et al. Rapamycin activates autophagy and improves myelination in explant cultures from neuropathicmice. J Neurosci. 2010 Aug 25;30(34):11388-97. [Content Brief]

[3]. Niu H, et al. Rapamycin potentiates cytotoxicity by RP-56976 possibly through downregulation of Survivin in lung cancer cells. J Exp Clin Cancer Res. 2011 Mar 10;30:28. [Content Brief]

[4]. Zhang JW, et al. Metformin synergizes with rapamycin to inhibit the growth of pancreatic cancer in vitro and in vivo. Oncol Lett. 2018 Feb;15(2):1811-1816. [Content Brief]

[5]. Svensson JE, et al. Evaluating the effect of rapamycin treatment in Alzheimer's disease and aging using in vivo imaging: the ERAP phase IIa clinical study protocol. BMC Neurol. 2024 Apr 4;24(1):111. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.0939 mL	5.4694 mL	10.9389 mL	27.3472 mL
	5 mM	0.2188 mL	1.0939 mL	2.1878 mL	5.4694 mL
	10 mM	0.1094 mL	0.5469 mL	1.0939 mL	2.7347 mL
	15 mM	0.0729 mL	0.3646 mL	0.7293 mL	1.8231 mL
	20 mM	0.0547 mL	0.2735 mL	0.5469 mL	1.3674 mL
	25 mM	0.0438 mL	0.2188 mL	0.4376 mL	1.0939 mL
	30 mM	0.0365 mL	0.1823 mL	0.3646 mL	0.9116 mL
	40 mM	0.0273 mL	0.1367 mL	0.2735 mL	0.6837 mL
	50 mM	0.0219 mL	0.1094 mL	0.2188 mL	0.5469 mL
	60 mM	0.0182 mL	0.0912 mL	0.1823 mL	0.4558 mL
	80 mM	0.0137 mL	0.0684 mL	0.1367 mL	0.3418 mL
	100 mM	0.0109 mL	0.0547 mL	0.1094 mL	0.2735 mL

Rapamycin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Rapamycin (Synonyms: Sirolimus; AY-22989; NSC 226080)

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Other Forms of Rapamycin:

1338 Publications Citing Use of MCE Rapamycin

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•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas mTOR:

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Ver todos los productos específicos de isoformas Antibiotic:

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Complete Stock Solution Preparation Table

Rapamycin Related Classifications

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Rapamycin (Synonyms: Sirolimus; AY-22989; NSC 226080)

Revisión del cliente

Other Forms of Rapamycin:

Rapamycin Related Antibodies

1338 Publications Citing Use of MCE Rapamycin

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas mTOR:

Ver todos los productos específicos de isoformas Endogenous Metabolite:

Ver todos los productos específicos de isoformas Antibiotic:

Actividad biológica

Pureza y Documentación

Referencias

Revisión del cliente

Rapamycin Related Antibodies

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

Rapamycin Related Classifications

Keywords:

Productos vistos recientemente:

Revisión del cliente

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report