Search Result

Results for "

chromogenic+substrate

" in MedChemExpress (MCE) Product Catalog:

By Targets:	Amyloid-β (1) Angiotensin-converting Enzyme (ACE) (2) Bacterial (1) Biochemical Assay Reagents (40) Caspase (6) Cathepsin (1) Cholinesterase (ChE) (3) Cytochrome P450 (3) DNA Stain (1) Endogenous Metabolite (6) Factor Xa (2) Fluorescent Dye (64) Glycosidase (3) HCV Protease (1) HIV Protease (1) Isotope-Labeled Compounds (1) Lipase (3) MMP (2) MOFs (1) NO Synthase (1) Phosphatase (5) Phospholipase (2) Plasminogen/Plasmin (2) Poly(ADP-ribose) Glycohydrolase (PARG) (1) Protease Activated Receptor (PAR) (1) Reactive Oxygen Species (ROS) (5) Reference Standards (2) SARS-CoV (1) Ser/Thr Protease (9) Thrombin (4) β-glucuronidase (3)
By Research Areas:	Cancer (12) Cardiovascular Disease (5) Infection (6) Inflammation/Immunology (2) Metabolic Disease (14) Neurological Disease (1)
Oligonucleotides:	Guanosine (1) Nucleoside Analogs (1)

153

Inhibitors & Agonists

Fluorescent Dyes

Biochemical Assay Reagents

Peptides

Natural
Products

Isotope-Labeled Compounds

Oligonucleotides

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-W011411

4-Nitrophenyl a-D-glucopyranoside	Glycosidase	Others
4-Nitrophenyl a-D-glucopyranoside is a chromogenic substrate for α-glucosidase. 4-Nitrophenyl a-D-glucopyranoside can be used to measure of α-glucosidase activity .

HY-15928

4-Nitrophenyl phosphate disodium 1 Publications Verification p-Nitrophenyl phosphate disodium	Fluorescent Dye	Others
4-Nitrophenyl phosphate (p-Nitrophenyl phosphate) disodium is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.

HY-101895

Fluorescein di(β-D-galactopyranoside) 5+ Cited Publications FDG	Fluorescent Dye	Others
Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λ_ex=485 nm, λ_em=535 nm).

HY-D0995

7-Methyl-6-thioguanosine 2 Publications Verification MESG	Fluorescent Dye	Others
7-Methyl-6-thioguanosine (MESG) is a chromogenic substrate that can be converted to 7-methyl-6-thioguanine in the presence of purine nucleoside phosphorylase and inorganic phosphate. 7-Methyl-6-thioguanosine can be used to quantify inorganic phosphate. 7-Methyl-6-thioguanosine can also be used to determine the activities of purine nucleoside phosphorylase and protein phosphatases .

HY-P2685

MeOSuc-Ala-Ala-Pro-Val-pNA	Fluorescent Dye	Others
MeOSuc-Ala-Ala-Pro-Val-pNA is a sensitive chromogenic substrate for human leukocyte and porcine pancreatic elastase .

HY-15909

BCIP BCIP p-toluidine salt; X-phosphate p-toluidine salt	Fluorescent Dye	Others
BCIP(BCIP p-toluidine salt; X-phosphate p-toluidine salt) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity .

HY-W013168

4-Nitrophenyl palmitate 4-Nitrophenyl hexadecanoate; p-Nitrophenyl Palmitate; pNpp	Lipase	Others
4-Nitrophenyl palmitate (4-Nitrophenyl hexadecanoate) is a chromogenic substrate for lipases and esterases. Upon enzymatic hydrolysis, 4-Nitrophenyl palmitate releases p-nitrophenol, which can be quantified by colorimetric detection at 410 nm as a measure of enzymatic activity. 4-Nitrophenyl palmitate is used to characterize the activity of various bacterial and mammalian enzymes, including those from Burkholderia and porcine pancreatic lipase .

HY-123275B

H-D-Phe-Pip-Arg-pNA acetate 1 Publications Verification S-2238 acetate	Fluorescent Dye	Others
H-D-Phe-Pip-Arg-pNA (S-2238) acetate, a chromogenic substrate, is patterned after the N-terminal portion of the A alpha chain of fibrinogen, which is the natural substrate of thrombin. H-D-Phe-Pip-Arg-pNA acetate is specific for thrombin and is used to measure antithrombin-heparin cofactor (AT-III). The AT-III assay using H-D-Phe-Pip-Arg-pNA acetate is sensitive, accurate, and easy to perform .

HY-123275A

H-D-Phe-Pip-Arg-pNA hydrochloride 1 Publications Verification S-2238 hydrochloride	Fluorescent Dye	Others
H-D-Phe-Pip-Arg-pNA (S-2238) hydrochloride, a chromogenic substrate, is patterned after the N-terminal portion of the A alpha chain of fibrinogen, which is the natural substrate of thrombin. H-D-Phe-Pip-Arg-pNA hydrochloride is specific for thrombin and is used to measure antithrombin-heparin cofactor (AT-III). The AT-III assay using H-D-Phe-Pip-Arg-pNA hydrochloride is sensitive, accurate, and easy to perform .

HY-P3936

Endotoxin Substrate	Fluorescent Dye	Others
Endotoxin Substrate (Boc-LGR-pNA) is a chromogenic substrate can be used in quantitative assays of endotoxin .

HY-W039892

4-Nitrophenyl β-D-glucuronide 1 Publications Verification pNPG_1	β-glucuronidase	Others
4-Nitrophenyl β-D-glucuronide (pNPG_1) is aderivative of 4-Nitrophenol. 4-Nitrophenyl β-D-glucuronide is the chromogenic substrate for β-glucuronidase .

HY-114522

X-alpha-Gal	Biochemical Assay Reagents	Others
X-alpha-Gal is a chromogenic substrate that is used to screen colonies with high activity of β-galactopyranoside. X-alpha-Gal also detects α-galactosidase activity. X-alpha-Gal can be used in yeast two hybrid screening for MEL1 gene activation. X-alpha-Gal can be used with Matchmaker GAL4-based products to confirm protein interactions .

HY-D0055

3-Cyano-7-ethoxycoumarin 1 Publications Verification	Cytochrome P450	Others
3-Cyano-7-ethoxycoumarin is a fluorescent cytochrome P-450 substrate that generates a blue fluorescent product upon enzymatic cleavage. 3-Cyano-7-ethoxycoumarin is used to detect the activity of mixed-function oxidases in isolated rat hepatocytes. 3-Cyano-7-ethoxycoumarin serves as a biological dye and indicator for research .

HY-P4202

Suc-Ala-Glu-Pro-Phe-pNA Suc-AEPF-pNA	Biochemical Assay Reagents	Others
Suc-Ala-Glu-Pro-Phe-pNA (Suc-AEPF-pNA) is a chromogenic substrate for the peptidyl-prolyl isomerase Pin1. Suc-Ala-Glu-Pro-Phe-pNA is used to evaluate the inhibitory effect of target compounds on Pin1, the catalytic activity of Pin1, and other related assays .

HY-W016188

1-Naphthyl acetate	Cholinesterase (ChE)	Others
1-Naphthyl acetate is an attractive chromogenic substrate for the detection of erythrocyte acetylcholinesterase (AChE) activity. 1-Naphthyl acetate has the potential to detect organophosphorus pesticide (OP) poisoning .

HY-15933

TOPS 1 Publications Verification	Biochemical Assay Reagents	Others Metabolic Disease
TOPS is a chromogenic substrate. TOPS undergoes an oxidative coupling reaction with 4-aminoantipyrine in the presence of H₂O₂ and nanocrystalline cobalt selenide. TOPS is used in studies related to uric acid detection .

HY-16710

GPDA 1 Publications Verification GPN; Glycylproline p-nitroanilide tosylate	Fluorescent Dye	Others
GPDA (GPN; Glycylproline p-nitroanilide tosylate) is a chromogenic substrate of prolyl dipeptidyl aminopeptidase. GPDA is used to detect the activity of prolyl dipeptidyl aminopeptidase in human serum .

HY-P3949

*Fluorescent Substrate* for Glu-Specific Proteases**	Fluorescent Dye	Others
Fluorescent Substrate for Glu-Specific Proteases is a V8 protease-Specific chromogenic substrate .

HY-153829

CH3OCO-D-CHA-Gly-Arg-pNA acetate	Factor Xa	Cardiovascular Disease
CH3OCO-D-CHA-Gly-Arg-pNA acetate is a chromogenic substrate for factor Xa .

HY-137878

2-O-(p-Nitrophenyl)-α-D-N-acetylneuraminic acid PNP-α-NeuNAc	Biochemical Assay Reagents	Others
2-O-(p-Nitrophenyl)-α-D-N-acetylneuraminic acid (PNP-α-NeuNAc) is a classic chromogenic substrate for neuraminidase. 2-O-(p-Nitrophenyl)-α-D-N-acetylneuraminic acid releases p-nitrophenol upon enzymatic hydrolysis, allowing quantification of enzyme activity and inhibitory effects via spectrophotometry. 2-O-(p-Nitrophenyl)-α-D-N-acetylneuraminic acid (PNP-α-NeuNAc) acts as a sialyl donor in the process of enzyme-catalyzed trans-sialylation .

HY-52112

BCDA 1 Publications Verification 5-bromo-4-chloroindoxyl acetate	Fluorescent Dye	Others
BCDA (5-bromo-4-chloroindoxyl acetate) is a chromogenic substrate of esterase used to potently detect the activity of esterase .

HY-137799

NOBA	Biochemical Assay Reagents	Others
NOBA is a synthetic chromogenic substrate that can be used to detect the enzyme activity of AplTX-II. NOBA can be used in the research of phospholipid hydrolysis .

HY-W127769

Rose-β-D-Gal Salmon-Gal	Fluorescent Dye	Others
Rose-β-D-Gal is a chromogenic substrate, is also a β-galactosidase substrate. Rose-β-D-Gal creates a pink/magenta color after the reaction and has been used for detection of β-gal activity .

HY-100045

p-Nitrophenyl phosphorylcholine 4-Nitrophenylphosphorylcholine; 4-Nitrophenylphosphorylcholine; O-(4-Nitrophenylphosphoryl)choline	Endogenous Metabolite	Metabolic Disease
p-Nitrophenyl phosphorylcholine (4-Nitrophenylphosphorylcholine) is a chromogenic substrate that is used to measure phospholipase C (PLC) activity. Hydrolysis of p-nitrophenylphosphorylcholine by PLC results in the liberation of p-nitrophenol, which can be measured at 405 nm at pH 7.2-7.5.

HY-D1213

4-Nitrocatechol sulfate dipotassium salt Dipotassium nitrocatechol sulfate	Biochemical Assay Reagents	Others
4-Nitrocatechol sulfate potassium salt (Dipotassium nitrocatechol sulfate) is an aromatic sulfate that can serve as a chromogenic substrate for sulfatase. 4-Nitrocatechol sulfate potassium salt can be used to determine the activities of glycosaminoglycan (GAG) degrading enzymes aryl sulfatase B and exonucleosidase .

HY-W009454

Potassium p-nitrophenyl sulfate 4-Nitopheyl Sulfate potassium	Fluorescent Dye	Others
Potassium p-nitrophenyl sulfate (4-Nitophenyl Sulfate potassium) serves as a chromogenic substrate for arylsulfatases. Potassium p-nitrophenyl sulfate releases nitrophenol under the cleavage action of arylsulfatases, and the activity of arylsulfatases can be quantitatively determined via colorimetric detection at 400 nm. Potassium p-nitrophenyl sulfate is commonly used in enzyme inhibition assays and metabolite analog-related studies .

HY-W015996

4-Nitrophenyl-N-acetyl-α-D-galactosaminide pNP-GalNAc	Fluorescent Dye	Others
4-Nitrophenyl-N-acetyl-α-D-galactosaminide (pNP-GalNAc) can be used as a chromogenic substrate for N-acetyl-D-galactosaminase. 4-Nitrophenyl-N-acetyl-α-D-galactosaminide is cleaved by N-acetyl-D-galactosaminase to generate a yellow solution. The enzyme activity is quantitatively determined by absorbance detection .

HY-P10094

β-Ala-Gly-Arg-pNA	Fluorescent Dye	Others
β-Ala-Gly-Arg-pNA is a chromogenic substrate of thrombin with pNA a strong absorbance at 405 nm .

HY-P4460

AAA-pNA	Ser/Thr Protease	Cancer
AAA-pNA is a chromogenic substrate of Tripeptidyl-peptidase II. AAA-pNA can be used to test Tripeptidyl-peptidase II activity .

HY-P4518

Z-Val-Gly-Arg-pNA	Ser/Thr Protease	Others
Z-Val-Gly-Arg-pNA is a chromogenic substrate for urokinase. Z-Val-Gly-Arg-pNA can be used for determination of urokinase activity .

HY-P4453

Boc-Ala-Ala-Asp-pNA	Caspase	Cancer
Boc-Ala-Ala-Asp-pNA is a chromogenic substrate of granzyme B. Boc-Ala-Ala-Asp-pNA can be used to test functional activity of granzyme B .

HY-123275C

H-D-Phe-Pip-Arg-pNA dihydrochloride S-2238 dihydrochloride	Fluorescent Dye	Others
H-D-Phe-Pip-Arg-pNA (S-2238) dihydrochloride, a chromogenic substrate, is patterned after the N-terminal portion of the A alpha chain of fibrinogen, which is the natural substrate of thrombin. H-D-Phe-Pip-Arg-pNA dihydrochloride is specific for thrombin and is used to measure antithrombin-heparin cofactor (AT-III). The AT-III assay using H-D-Phe-Pip-Arg-pNA dihydrochloride is sensitive, accurate, and easy to perform .

HY-W328317

Fast garnet GBC sulfate salt, 90% Azoic diazo No. 4, 90%	Biochemical Assay Reagents	Others
Fast garnet GBC sulfate salt, 90% is a chromogenic substrate for alkaline phosphatase.

HY-W341421

3-Indoxyl butyrate	Biochemical Assay Reagents	Others
3-Indoxyl butyrate is a chromogenic substrate for CES (carboxylesterase), producing a blue precipitate upon cleavage.

HY-P4484

Suc-AAPK-pNA	Biochemical Assay Reagents	Others
Suc-AAPK-pNA is a chromogenic substrate for the determination of serine/threonine kinase activity and enzyme kinetic parameters .

HY-P3126

Suc-Ile-Glu(γ-pip)-Gly-Arg-pNA hydrochloride	Factor Xa	Others
Suc-Ile-Glu(γ-pip)-Gly-Arg-pNA hydrochloride is a factor Xa specific chromogenic substrate .

HY-P10145

Ac-LEVD-pNA	Biochemical Assay Reagents Caspase	Others
Ac-LEVD-pNA is a chromogenic substrate for caspase-4 and can be used to detect caspase-4 activity .

HY-P3934

*HIV Protease Substrate* I**	HIV Protease	Infection
HIV Protease Substrate I is a chromogenic substrate of HIV-1 protease. HIV Protease Substrate I has the cleavage site of HIV protease .

HY-123275

H-D-Phe-Pip-Arg-pNA S-2238	Fluorescent Dye	Others
H-D-Phe-Pip-Arg-pNA (S-2238), a chromogenic substrate, is patterned after the N-terminal portion of the A alpha chain of fibrinogen, which is the natural substrate of thrombin. H-D-Phe-Pip-Arg-pNA is specific for thrombin and is used to measure antithrombin-heparin cofactor (AT-III). The AT-III assay using H-D-Phe-Pip-Arg-pNA is sensitive, accurate, and easy to perform .

HY-P4039

Ac-EEVVAC-pNA	HCV Protease	Infection
Ac-EEVVAC-pNA is a chromogenic substrate for a continuous spectrophotometric assay of HCV NS3 protease. The sequence EEVVAC is derived from the 5A-5B cleavage junction of the HCV polyprotein .

HY-P4458

Boc-QAR-pNA	Ser/Thr Protease	Cancer
Boc-QAR-pNA is a chromogenic substrate of trypsin. Boc-QAR-pNA can be used to test trypsin activity .

HY-P4482

Suc-AAPE-pNA	Bacterial	Infection
Suc-AAPE-pNA is a chromogenic substrate of glutamyl endopeptidase. Suc-AAPE-pNA can be used to test glutamyl endopeptidase activity .

HY-P4470

Phe-Pro-Ala-pNA	Ser/Thr Protease	Cancer
Phe-Pro-Ala-pNA is a chromogenic substrate of tripeptidyl peptidase. Phe-Pro-Ala-pNA can be used to test tripeptidyl peptidase activity .

HY-P4464

Ala-Phe-Pro-pNA	Ser/Thr Protease	Cancer
Ala-Phe-Pro-pNA is a chromogenic substrate of tripeptidyl peptidase. Ala-Phe-Pro-pNA can be used to test tripeptidyl peptidase activity .

HY-138143

N-(p-Tosyl)-GPR-pNA acetate Chromozym-TH	Fluorescent Dye	Others
N-(p-Tosyl)-GPR-pNA acetate (Chromozym-TH) is a chromogenic substrate targeting the synthetic peptides Hirunorm IV and Hirunorm V and can be used to detect the dissociation constants (KI) of both peptides. Hirunorm IV and Hirunorm V are reversible inhibitors of amidolytic thrombin activity. By varying the peptide concentration at a fixed concentration of the chromogenic substrate N-(p-Tosyl)-GPR-pNA acetate, the dissociation constants determined were 0.134 nM (Hirunorm IV) and 0.245 nM (Hirunorm V) .

HY-P4464A

Ala-Phe-Pro-pNA TFA	Ser/Thr Protease	Cancer
Ala-Phe-Pro-pNA TFA is a chromogenic substrate of tripeptidyl peptidase. Ala-Phe-Pro-pNA TFA can be used to test tripeptidyl peptidase activity .

HY-W016188R

1-Naphthyl acetate (Standard)	Reference Standards Cholinesterase (ChE)	Others
1-Naphthyl acetate is an attractive chromogenic substrate for the detection of erythrocyte acetylcholinesterase (AChE) activity. 1-Naphthyl acetate has the potential to detect organophosphorus pesticide (OP) poisoning .

HY-138653

Lipase Substrate DGGR; 1,2-o-Dilauryl-rac-glycero-3-glutaric acid (6′-methylresorufin) ester	Lipase	Metabolic Disease
Lipase Substrate is a chromogenic substrate of lipase to detect activity . It is used in colorimetric methods to measure lipase activity. It produces a red-purple compound, methylresorufin, upon digestion by the lipase enzyme.

HY-P6027A

H-D-CHA-Ala-Arg-pNA diacetate	Fluorescent Dye	Others
H-D-CHA-Ala-Arg-pNA diacetate is the diacetate form of H-D-CHA-Ala-Arg-pNA (HY-P6027). H-D-CHA-Ala-Arg-pNA diacetate is a chromogenic substrate for the amidolytic assay .

HY-113245

3-Indolyl-β-D-glucuronide	Endogenous Metabolite	Others
3-Indolyl-β-D-glucuronide, a chromogenic substrate for β-D-glucuronidase, employs in the detection and enumeration of E. coli, yielding a blue precipitate upon cleavage. 3-Indolyl-β-D-glucuronide in? patients' plasma act as a new indicator of renal failure .

Cat. No.	Product Name	Type

HY-12591B

D-Luciferin potassium

105+ Cited Publications

D-(-)-Luciferin potassium; Firefly luciferin potassium; Beetle Luciferin potassium

Fluorescent Dyes

D-luciferin potassium is the natural substrate of the enzyme luciferase (Luc) that catalyzes the production of the typical yellowgreen light of fireflies. The 560 nm chemiluminescence from this reaction peaks within seconds, with light output that is proportional to luciferase concentration when the substrate luciferin is present in excess. The luciferase (luc) gene is a popular reporter gene for research and agent screening. Chemiluminescent techniques are virtually background-free, making the luc reporter gene ideal for detecting low-level gene expression. As little as 0.02 pg of luciferase can be reliably measured in a standard scintillation counter. In addition to its role as a reporter of gene expression, luciferase is commonly used in an extremely sensitive assay for ATP . We offer the firefly luciferase (HY-P1004A), luciferin free acid (HY-12591A), as well as its water-soluble sodium salts (HY-12591) and potassium salts (HY-12591B) .

HY-12591

D-Luciferin sodium

105+ Cited Publications

D-(-)-Luciferin sodium; Firefly luciferin sodium; Beetle Luciferin sodium

Fluorescent Dyes

D-luciferin sodium is the natural substrate of the enzyme luciferase (Luc) that catalyzes the production of the typical yellowgreen light of fireflies. The 560 nm chemiluminescence from this reaction peaks within seconds, with light output that is proportional to luciferase concentration when the substrate luciferin is present in excess. The luciferase (luc) gene is a popular reporter gene for research and agent screening. Chemiluminescent techniques are virtually background-free, making the luc reporter gene ideal for detecting low-level gene expression. As little as 0.02 pg of luciferase can be reliably measured in a standard scintillation counter. In addition to its role as a reporter of gene expression, luciferase is commonly used in an extremely sensitive assay for ATP . We of er the firefly luciferase (HY-P1004A), luciferin free acid (HY-12591A), as well as its water-soluble sodium salts (HY-12591) and potassium salts (HY-12591B) .

HY-15924

Thiazolyl Blue Maximum Cited Publications 182 Publications Verification MTT; Thiazolyl Blue Tetrazolium bromide; Methylthiazolyldiphenyl-tetrazolium bromide	Fluorescent Dyes
Thiazolyl Blue (MTT) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis .

HY-12591A

D-Luciferin

105+ Cited Publications

D-(-)-Luciferin; Firefly luciferin; Beetle Luciferin

Fluorescent Dyes

D-luciferin is the natural substrate of the enzyme luciferase (Luc) that catalyzes the production of the typical yellowgreen light of fireflies. The 560 nm chemiluminescence from this reaction peaks within seconds, with light output that is proportional to luciferase concentration when the substrate luciferin is present in excess. The luciferase (luc) gene is a popular reporter gene for research and agent screening. Chemiluminescent techniques are virtually background-free, making the luc reporter gene ideal for detecting low-level gene expression. As little as 0.02 pg of luciferase can be reliably measured in a standard scintillation counter. In addition to its role as a reporter of gene expression, luciferase is commonly used in an extremely sensitive assay for ATP . We of er the firefly luciferase (HY-P1004A), luciferin free acid (HY-12591A), as well as its water-soluble sodium salts (HY-12591) and potassium salts (HY-12591B) .

HY-15902

ABTS diammonium salt 10+ Cited Publications AzBTS-(NH4)2	Fluorescent Dyes
ABTS diammonium salt (AzBTS-(NH4)2) is a substrate for horseradish peroxidase (HRP) conjugate. ABTS diammonium salt can be used to evaluate antioxidant capacity .

HY-15925

Nitro blue tetrazolium chloride 5+ Cited Publications NBT	Fluorescent Dyes
Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures .

HY-D0831

WST-8 15+ Cited Publications	Fluorescent Dyes
WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.

HY-15934

X-GAL 5 Publications Verification BCIG	Fluorescent Dyes
X-GAL (BCIG) is a widely used chromogenic β-galactosidase substrate. X-GAL is a colorless compound until cleaved by β-galactosidase, at which point X-GAL turns to an insoluble and detectable blue compound, making X-GAL particularly useful in techniques such as blue-white screening for cloning in bacteria. X-GAL can also be used for detection of β-galactosidase activity .

HY-112641A

AkaLumine hydrochloride 5 Publications Verification	Fluorescent Dyes
AkaLumine hydrochloride is a D-luciferin (HY-12591A) analogue with a K_m of 2.06 μM for recombinant firefly luciferase (Fluc) protein. AkaLumine hydrochloride emits near-infrared (NIR) light (λmax=677 nm) in reactions with native Fluc. AkaLumine hydrochloride has high tissue-penetration and increases detection sensitivity from deep-tissue targets .

HY-N0116

Hematoxylin 10+ Cited Publications Natural Black 1; Haematoxylin	Fluorescent Dyes
Hematoxylin (Natural Black 1), a naturally occurring flavonoid compound derived from Caesalpinia sappan Linn.. Hematoxylin is a nuclear stain in histology and is also a potent Aβ42 fibrillogenesis inhibitor with an IC₅₀ of 1.6 μM.

HY-D1670

Z-Gly-Pro-AMC 3 Publications Verification	Fluorescent Dyes
Z-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-Amino-4-methylcoumarin (HY-D0027). (λ_ex=380 nm, λ_em=465 nm) .

HY-15928

4-Nitrophenyl phosphate disodium 1 Publications Verification p-Nitrophenyl phosphate disodium	Fluorescent Dyes
4-Nitrophenyl phosphate (p-Nitrophenyl phosphate) disodium is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.

HY-101895

Fluorescein di(β-D-galactopyranoside) 5+ Cited Publications FDG	Fluorescent Dyes
Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λ_ex=485 nm, λ_em=535 nm).

HY-111653

CycLuc1 2 Publications Verification	Fluorescent Dyes
CycLuc1 is a blood-brain barrier permeable luciferase substrate that displays near-infrared (NIR) emission with a peak luminescence wavelength of 599 nm. CycLuc1 can be used for in vivo bioluminescence imaging .

HY-D0994

4-Methylumbelliferyl phosphate 1 Publications Verification 4-MUP; MUP	Fluorescent Dyes
4-Methylumbelliferyl phosphate (4-MUP), an anionic organophosphate, is a acid and alkaline phosphatase fluorogenic substrate. 4-Methylumbelliferyl phosphate is also a nerve agent simulant .

HY-W010991

N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly

1 Publications Verification

FAPGG

Fluorescent Dyes

N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly (FAPGG) is a specific substrate of angiotensin converting enzyme (ACE) with a Ki of 2.546×10 ^-4 M. It is used as a chromogenic probe for quantitative detection of ACE activity. N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly can be hydrolyzed by ACE to generate N-[3-(2-furyl)acryloyl]-Phe (FAP) and Gly-Gly, and the ACE inhibitory effect is monitored by photometry. FAPGG competitively binds to the active center of ACE and is a key tool for screening ACE inhibitors such as Captopril (HY-B0368) and Dioscorin. Its reversible mechanism of action supports hypertension research and drug development targeting the renin-angiotensin system .

HY-P0019

Z-Gly-Gly-Arg-AMC Maximum Cited Publications 11 Publications Verification	Fluorescent Dyes
Z-Gly-Gly-Arg-AMC is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP) (Ex/Em = 390/480 nm) .

HY-15930

TMB

5+ Cited Publications

BM blue; Sure Blue TMB

Fluorescent Dyes

TMB is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922 .

HY-D0935A

4-Methylumbelliferyl-β-D-glucuronide hydrate 1 Publications Verification MUG	Fluorescent Dyes
4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λ_ex=362 nm, λ_em=445 nm). 4-Methylumbelliferyl-β-D-glucuronide hydrate has potential applications in detecting the activity of β-glucuronidase and the number of Escherichia coli .

HY-W127711

Fast Blue B Salt, Dye content ~95%

Fluorescent Dyes

Fast Blue B Salt, Dye content ~95% is a diazo dye, primarily used as a chromogenic substrate in biochemical analysis. Fast Blue B Salt, Dye content ~95% is the coupling agent for the histochemical demonstration of proteases. Fast Blue B Salt, Dye content ~95% can be used for the detection of acid phosphatase in Clostridium perfringens, and also for the determination of lipase activity. Fast Blue B Salt, Dye content ~95% reacts directly with the active hydroxyl groups in phenolic compounds to determine the content of phenolic substances .

HY-D0995

7-Methyl-6-thioguanosine 2 Publications Verification MESG	Fluorescent Dyes
7-Methyl-6-thioguanosine (MESG) is a chromogenic substrate that can be converted to 7-methyl-6-thioguanine in the presence of purine nucleoside phosphorylase and inorganic phosphate. 7-Methyl-6-thioguanosine can be used to quantify inorganic phosphate. 7-Methyl-6-thioguanosine can also be used to determine the activities of purine nucleoside phosphorylase and protein phosphatases .

HY-D1224

Chlorophenol red-β-D-galactopyranoside

2 Publications Verification

CPRG

Fluorescent Dyes

Chlorophenol red-β-D-galactopyranoside (CPRG) is an efficient and sensitive chromogenic substrate for β-galactosidase (HY-P2869), widely used in colorimetric assays. Chlorophenol red-β-D-galactopyranoside itself appears pale yellow. When it is specifically hydrolyzed by β-galactosidase, it releases chlorophenol red. The released chlorophenol red turns purple-red under alkaline or neutral pH conditions. This color change from yellow to red can be quantitatively detected at wavelengths of 540-572 nm using visible spectrophotometry .

HY-P2685

MeOSuc-Ala-Ala-Pro-Val-pNA	Fluorescent Dyes
MeOSuc-Ala-Ala-Pro-Val-pNA is a sensitive chromogenic substrate for human leukocyte and porcine pancreatic elastase .

HY-15909

BCIP BCIP p-toluidine salt; X-phosphate p-toluidine salt	Fluorescent Dyes
BCIP(BCIP p-toluidine salt; X-phosphate p-toluidine salt) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity .

HY-W013168

4-Nitrophenyl palmitate

4-Nitrophenyl hexadecanoate; p-Nitrophenyl Palmitate; pNpp

Fluorescent Dyes

4-Nitrophenyl palmitate (4-Nitrophenyl hexadecanoate) is a chromogenic substrate for lipases and esterases. Upon enzymatic hydrolysis, 4-Nitrophenyl palmitate releases p-nitrophenol, which can be quantified by colorimetric detection at 410 nm as a measure of enzymatic activity. 4-Nitrophenyl palmitate is used to characterize the activity of various bacterial and mammalian enzymes, including those from Burkholderia and porcine pancreatic lipase .

HY-P1003

Ac-DEVD-AMC 4 Publications Verification	Fluorescent Dyes
Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm .

HY-15914

DHBS DCHBS	Fluorescent Dyes
DHBS (3, 5-dichloro-2-hydroxybenzenesulfonate) is used in conjunction with 4-aminoantipyrine (4-AAP) (HY-W100990) and hydrogen peroxide (H₂O₂) for chromogenic quantitation of peroxidase in coupled enzymatic reactions. Component of Trinder reagent for use with peroxidase to measure generation of hydrogen peroxide in automated systems .

HY-15930A

TMB dihydrochloride

5+ Cited Publications

BM blue dihydrochloride; Sure Blue TMB dihydrochloride

Fluorescent Dyes

TMB dihydrochloride is the dihydrochloride form of TMB (HY-15930). TMB dihydrochloride is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB dihydrochloride can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB dihydrochloride can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922 .

HY-D0055

3-Cyano-7-ethoxycoumarin 1 Publications Verification	Fluorescent Dyes
3-Cyano-7-ethoxycoumarin is a fluorescent cytochrome P-450 substrate that generates a blue fluorescent product upon enzymatic cleavage. 3-Cyano-7-ethoxycoumarin is used to detect the activity of mixed-function oxidases in isolated rat hepatocytes. 3-Cyano-7-ethoxycoumarin serves as a biological dye and indicator for research .

HY-D0145

7-Ethoxyresorufin 1 Publications Verification Resorufin ethyl ether	Fluorescent Dyes
7-Ethoxyresorufin (Resorufin ethyl ether) is a fluorometric substrate and competitive inhibitor of cytochrome P450, especially CYP1A1. 7-Ethoxyresorufin also inhibits NO synthase .

HY-P4202

Suc-Ala-Glu-Pro-Phe-pNA Suc-AEPF-pNA	Fluorescent Dyes
Suc-Ala-Glu-Pro-Phe-pNA (Suc-AEPF-pNA) is a chromogenic substrate for the peptidyl-prolyl isomerase Pin1. Suc-Ala-Glu-Pro-Phe-pNA is used to evaluate the inhibitory effect of target compounds on Pin1, the catalytic activity of Pin1, and other related assays .

HY-P0019A

Z-Gly-Gly-Arg-AMC acetate Maximum Cited Publications 11 Publications Verification	Fluorescent Dyes
Z-Gly-Gly-Arg-AMC acetate is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP) (Ex/Em = 390/480 nm) .

HY-P1093

Ac-LEHD-AFC 3 Publications Verification	Fluorescent Dyes
Ac-LEHD-AFC is a fluorescent substrate for caspase-9, can be used to assess the activity of caspase .

HY-16710

GPDA 1 Publications Verification GPN; Glycylproline p-nitroanilide tosylate	Fluorescent Dyes
GPDA (GPN; Glycylproline p-nitroanilide tosylate) is a chromogenic substrate of prolyl dipeptidyl aminopeptidase. GPDA is used to detect the activity of prolyl dipeptidyl aminopeptidase in human serum .

HY-129096

IDT307 2 Publications Verification	Fluorescent Dyes
IDT307, an analog of the organic cation MPP+, is a specific fluorescent substrate for DAT (fluorescent substrate APP+) .

HY-148073

MeO-Succ-Arg-Pro-Tyr-AMC TFA	Fluorescent Dyes
MeO-Succ-Arg-Pro-Tyr-AMC TFA is a fluorogenic peptide substrate for chymotrypsin-like proteases that detects protease activity . (measured at 460 nm)

HY-52112

BCDA 1 Publications Verification 5-bromo-4-chloroindoxyl acetate	Fluorescent Dyes
BCDA (5-bromo-4-chloroindoxyl acetate) is a chromogenic substrate of esterase used to potently detect the activity of esterase .

HY-W127769

Rose-β-D-Gal Salmon-Gal	Fluorescent Dyes
Rose-β-D-Gal is a chromogenic substrate, is also a β-galactosidase substrate. Rose-β-D-Gal creates a pink/magenta color after the reaction and has been used for detection of β-gal activity .

HY-100045

p-Nitrophenyl phosphorylcholine 4-Nitrophenylphosphorylcholine; 4-Nitrophenylphosphorylcholine; O-(4-Nitrophenylphosphoryl)choline	Fluorescent Dyes
p-Nitrophenyl phosphorylcholine (4-Nitrophenylphosphorylcholine) is a chromogenic substrate that is used to measure phospholipase C (PLC) activity. Hydrolysis of p-nitrophenylphosphorylcholine by PLC results in the liberation of p-nitrophenol, which can be measured at 405 nm at pH 7.2-7.5.

HY-D1213

4-Nitrocatechol sulfate dipotassium salt Dipotassium nitrocatechol sulfate	Fluorescent Dyes
4-Nitrocatechol sulfate potassium salt (Dipotassium nitrocatechol sulfate) is an aromatic sulfate that can serve as a chromogenic substrate for sulfatase. 4-Nitrocatechol sulfate potassium salt can be used to determine the activities of glycosaminoglycan (GAG) degrading enzymes aryl sulfatase B and exonucleosidase .

HY-P0021A

D-Lys(Z)-Pro-Arg-pNA diacetate 1 Publications Verification Spectrozyme PCa; Chromozym Pca diacetate	Fluorescent Dyes
D-Lys(Z)-Pro-Arg-pNA diacetate is a chromogenic peptide substrate of activated protein C (APC) .

HY-D1634

Bz-FVR-AMC	Fluorescent Dyes
Bz-FVR-AMC is a fluorogenic substrate for procathepsin with a k_cat/K_m value of 1070 mM ^-1s ^-1. The high concentration of BZ-FVR-AMC inhibits the substrate .

HY-D1208

Alkaline phosphatase substrate	Fluorescent Dyes
Alkaline phosphatase substrate is a chemiluminescent substrate for alkaline phosphatase. Alkaline phosphatase substrate can be used for enzyme-linked immunoassays .

HY-146248

TFMU-ADPr

Fluorescent Dyes

TFMU-ADPr is a selective reporter substrate of SARS-CoV-2 Macro1 (IC₅₀=0.59 μM), with an excitation wavelength (λ_Ex) of 385 nm, and an emission wavelength (λ_Em) of 502 nm (or 495 nm). TFMU-ADPr can also undergo enzymatic hydrolysis with Poly(ADP-ribose) Glycohydrolase (PARG) sourced from human, Tetrahymena thermophila and ADP-ribosylhydrolase 3 from human to release fluorophores, thereby directly reporting total poly (ADP-ribose) hydrolase activity. TFMU-ADPr binds to the ADPr-binding site of SARS-CoV-2 Macro1, and its TFMU moiety inserts into the narrow hydrophobic groove of this protein. TFMU-ADPr can thus be used to evaluate small-molecule inhibitors targeting PAR hydrolases under in vitro conditions, to investigate the regulatory mechanisms of ADP-ribosyl catabolic enzymes, or to detect PAR hydrolase activity in whole-cell lysate assays. TFMU-ADPr is also applicable to COVID-19-related research .

HY-W015996

4-Nitrophenyl-N-acetyl-α-D-galactosaminide pNP-GalNAc	Fluorescent Dyes
4-Nitrophenyl-N-acetyl-α-D-galactosaminide (pNP-GalNAc) can be used as a chromogenic substrate for N-acetyl-D-galactosaminase. 4-Nitrophenyl-N-acetyl-α-D-galactosaminide is cleaved by N-acetyl-D-galactosaminase to generate a yellow solution. The enzyme activity is quantitatively determined by absorbance detection .

HY-15930C

TMB monosulfate

5+ Cited Publications

BM blue monosulfate; Sure Blue TMB monosulfate

Fluorescent Dyes

TMB monosulfate is the monosulfate form of TMB (HY-15930). TMB monosulfate is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB monosulfate can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB monosulfate can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922 .

HY-P1448

Ac-Leu-Arg-AMC	Fluorescent Dyes
Ac-Leu-Arg-AMC is a fluorogenic peptide substrate.

HY-P3120

Mca-Pro-Leu-Ala-Cys(Mob)-Trp-Ala-Arg-Dap(Dnp)-NH2	Fluorescent Dyes
Mca-Pro-Leu-Ala-Cys(Mob)-Trp-Ala-Arg-Dap(Dnp)-NH2 is a fluorogenic substrate for matrix metalloproteinase-14 (MMP-14). Mca-Pro-Leu-Ala-Cys(Mob)-Trp-Ala-Arg-Dap(Dnp)-NH2, upon cleavage by MMP-14, releases 7-methoxycoumarin-4-acetyl (Mca) (Ex/Em = 328/420 nm).

HY-P4039

Ac-EEVVAC-pNA	Fluorescent Dyes
Ac-EEVVAC-pNA is a chromogenic substrate for a continuous spectrophotometric assay of HCV NS3 protease. The sequence EEVVAC is derived from the 5A-5B cleavage junction of the HCV polyprotein .

HY-D1026

CUG 3-Carboxyumbelliferyl-β-D-galactopyranoside	Fluorescent Dyes
CUG (3-Carboxyumbelliferyl-β-D-galactopyranoside) is a fluorogenic substrate (λ_ex=386, λ_em=445 nm, ε=32K).

Cat. No.	Product Name	Type

HY-15927

4-Nitrophenyl β-D-glucopyranoside

1 Publications Verification

Biochemical Assay Reagents

4-Nitrophenyl β-D-glucopyranoside is a chromogenic substrate for β-glucosidase. 4-Nitrophenyl β-D-glucopyranoside is converted to a colored product, p-nitrophenol that is easily detected spectrophotometrically at 405 nm when used in a β-glycosidase assay. 4-Nitrophenyl β-D-glucopyranoside is hydrolysed through intramolecular nucleophilic catalysis by the phosphate group in the 2-position. 4-Nitrophenyl β-D-glucopyranoside is promising for research of postmenopausal osteoporosis .

HY-W127711

Fast Blue B Salt, Dye content ~95%

Biochemical Assay Reagents

HY-15933

TOPS 1 Publications Verification	Biochemical Assay Reagents
TOPS is a chromogenic substrate. TOPS undergoes an oxidative coupling reaction with 4-aminoantipyrine in the presence of H₂O₂ and nanocrystalline cobalt selenide. TOPS is used in studies related to uric acid detection .

HY-137878

2-O-(p-Nitrophenyl)-α-D-N-acetylneuraminic acid

PNP-α-NeuNAc

Biochemical Assay Reagents

2-O-(p-Nitrophenyl)-α-D-N-acetylneuraminic acid (PNP-α-NeuNAc) is a classic chromogenic substrate for neuraminidase. 2-O-(p-Nitrophenyl)-α-D-N-acetylneuraminic acid releases p-nitrophenol upon enzymatic hydrolysis, allowing quantification of enzyme activity and inhibitory effects via spectrophotometry. 2-O-(p-Nitrophenyl)-α-D-N-acetylneuraminic acid (PNP-α-NeuNAc) acts as a sialyl donor in the process of enzyme-catalyzed trans-sialylation .

HY-D1208

Alkaline phosphatase substrate	Biochemical Assay Reagents
Alkaline phosphatase substrate is a chemiluminescent substrate for alkaline phosphatase. Alkaline phosphatase substrate can be used for enzyme-linked immunoassays .

HY-137779

5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside

Biochemical Assay Reagents

5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside, a chromogenic substrate for the detection of β-galactosidase activity. It is commonly used in molecular biology techniques such as gene expression analysis and reporter gene analysis. When β-galactosidase cleaves X-Gluc, a blue precipitate is produced, which can be observed by microscopy or other detection methods. X-Gluc has high sensitivity and specificity for the detection of β-galactosidase activity, making it a widely used tool in molecular biology research.

HY-W328317

Fast garnet GBC sulfate salt, 90% Azoic diazo No. 4, 90%	Biochemical Assay Reagents
Fast garnet GBC sulfate salt, 90% is a chromogenic substrate for alkaline phosphatase.

HY-W341421

3-Indoxyl butyrate	Biochemical Assay Reagents
3-Indoxyl butyrate is a chromogenic substrate for CES (carboxylesterase), producing a blue precipitate upon cleavage.

HY-137895

L-Arginine p-nitroanilide dihydrochloride, 98% H-Arg-pNA 2HCl, 98%	Biochemical Assay Reagents
L-Arginine p-nitroanilide dihydrochloride, 98% (H-Arg-pNA 2HCl, 98%) is a chromogenic substrate for cathepsin H and aminopeptidases.

HY-D1224A

Chlorophenol red β-D-galactopyranoside sodium

CPRG sodium

Biochemical Assay Reagents

Chlorophenol red-β-D-galactopyranoside (CPRG) sodium is an efficient and sensitive chromogenic substrate for β-galactosidase (HY-P2869), widely used in colorimetric assays. Chlorophenol red-β-D-galactopyranoside sodium itself appears pale yellow. When it is specifically hydrolyzed by β-galactosidase, it releases chlorophenol red. The released chlorophenol red turns purple-red under alkaline or neutral pH conditions. This color change from yellow to red can be quantitatively detected at wavelengths of 540-572 nm using visible spectrophotometry .

HY-137228

4-Nitrophenylmaltohexaoside	Biochemical Assay Reagents
4-Nitrophenylmaltohexaoside is a chromogenic substrate targeting α-amylase.

HY-W013758

TMB-PS sodium TMBZ-PS sodium	Biochemical Assay Reagents
TMB-PS (TMBZ-PS) sodium is a chromogenic substrate for the detection and quantification of peroxidase activity .

HY-W290273

2-Nitrophenyl-β-D-thiogalactopyranoside 2-Nitrophenyl-beta-D-thiogalactopyranoside	Biochemical Assay Reagents
2-Nitrophenyl-β-D-thiogalactopyranoside is a chromogenic substrate used to detect the presence of galactosidase in various biological and biochemical assays.

HY-138821

BCIP potassium	Biochemical Assay Reagents
BCIP (potassium) is a chromogenic substrate commonly used to detect the enzymatic activity of alkaline phosphatase. When dephosphorylated by alkaline phosphatase, BCIP produces a blue precipitate.

HY-W011655

p-Nitrophenyl α-D-xylopyranoside p-Nitrophenyl alpha-D-xylopyranoside	Biochemical Assay Reagents
p-Nitrophenyl α-D-xylopyranoside (p-Nitrophenyl alpha-D-xylopyranoside) is a chromogenic substrate for α-xylosidase .

HY-W291944

5-Bromo-4-chloro-3-indolyl-α-L-fucopyranoside	Biochemical Assay Reagents
5-Bromo-4-chloro-3-indolyl-α-L-fucopyranoside can be used as a chromogenic substrate for α-L-Fucosidase.

HY-137785

2-Chloro-4-nitrophenyl-β-D-maltotrioside	Biochemical Assay Reagents
2-Chloro-4-nitrophenyl-β-D-maltotrioside is a chromogenic substrate used in bioluminescent and fluorescent assays to detect α-amylase.

HY-W293502

2-Chloro-4-nitrophenyl-β-D-galactopyranoside 2-Chloro-4-nitrophenyl-beta-D-galactopyranoside	Biochemical Assay Reagents
2-Chloro-4-nitrophenyl-β-D-galactopyranoside is a chromogenic substrate used for testing enzyme activity. It is commonly used in molecular biology research to detect and measure the activity of β-galactosidase (biosynth: EC03318).

HY-W101382

5-Bromo-6-chloro-3-indolyl-N-acetyl-β-D-glucosaminide, 98%	Biochemical Assay Reagents
5-Bromo-6-chloro-3-indolyl-N-acetyl-β-D-glucosaminide, 98% is a chromogenic substrate used to measure β-galactosidase activity in food, enzyme substrates, and culture media.

HY-W104635

6-Bromo-2-naphthyl-β-D-galactopyranoside 6-Bromo-2-naphthyl-beta-D-galactopyranoside	Biochemical Assay Reagents
6-Bromo-2-naphthyl-β-D-galactopyranoside is a chromogenic substrate commonly used to measure β-galactosidase enzyme activity in food, enzyme substrates, and culture media. Upon hydrolysis by β-galactosidase, it generates a yellow precipitate indicating the enzyme's presence.

HY-W854392

3-Carboxyumbelliferyl-β-D-glucuronide dipotassium

Biochemical Assay Reagents

3-Carboxyumbelliferyl-β-D-glucuronide (dipotassium) is a chromogenic substrate with biological activity for enzyme activity detection. 3-Carboxyumbelliferyl-β-D-glucuronide (dipotassium) can be used to evaluate biological reactions associated with glycosidases. 3-Carboxyumbelliferyl-β-D-glucuronide (dipotassium) shows a wide range of application potentials in compound discovery and biomedical research.

HY-W698964

2′-Hydroxy-5′-nitrohexadecanamide

Biochemical Assay Reagents

2′-Hydroxy-5′-nitrohexadecanamide is a lysosomal hydrolase inhibitor and is a lipid containing pentadecanoic acid and a terminal nitrophenol in its structure. 2′-Hydroxy-5′-nitrohexadecanamide can cause intracellular lipid accumulation by inhibiting lysosomal hydrolase activity. Additionally, 2′-Hydroxy-5′-nitrohexadecanamide can be used to synthesize chromogenic substrates for measuring sphingolipase activity .

HY-W638077

6-Chloro-3-indoxyl caprylate	Biochemical Assay Reagents
6-Chloro-3-indoxyl caprylate is a chromogenic substrate with C8 activity for esterases.

HY-W587779

o-Nitrophenyl β-D-galactopyranoside-6-phosphate cyclohexylammonium	Biochemical Assay Reagents
o-Nitrophenyl β-D-galactopyranoside-6-phosphate cyclohexylammonium is a chromogenic substrate with activity for β-galactosidase detection. The use of o-Nitrophenyl β-D-galactopyranoside-6-phosphate cyclohexylammonium can help researchers quickly identify and quantify β-galactosidase activity.

Cat. No.	Product Name	Target	Research Area

HY-W010991

N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly 1 Publications Verification FAPGG	Angiotensin-converting Enzyme (ACE)	Others
N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly (FAPGG) is a specific substrate of angiotensin converting enzyme (ACE) with a Ki of 2.546×10 ^-4 M. It is used as a chromogenic probe for quantitative detection of ACE activity. N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly can be hydrolyzed by ACE to generate N-[3-(2-furyl)acryloyl]-Phe (FAP) and Gly-Gly, and the ACE inhibitory effect is monitored by photometry. FAPGG competitively binds to the active center of ACE and is a key tool for screening ACE inhibitors such as Captopril (HY-B0368) and Dioscorin. Its reversible mechanism of action supports hypertension research and drug development targeting the renin-angiotensin system .

HY-P0019

Z-Gly-Gly-Arg-AMC Maximum Cited Publications 11 Publications Verification	Fluorescent Dye Thrombin	Cardiovascular Disease
Z-Gly-Gly-Arg-AMC is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP) (Ex/Em = 390/480 nm) .

HY-P2685

MeOSuc-Ala-Ala-Pro-Val-pNA	Fluorescent Dye	Others
MeOSuc-Ala-Ala-Pro-Val-pNA is a sensitive chromogenic substrate for human leukocyte and porcine pancreatic elastase .

HY-123275B

H-D-Phe-Pip-Arg-pNA acetate 1 Publications Verification S-2238 acetate	Fluorescent Dye	Others
H-D-Phe-Pip-Arg-pNA (S-2238) acetate, a chromogenic substrate, is patterned after the N-terminal portion of the A alpha chain of fibrinogen, which is the natural substrate of thrombin. H-D-Phe-Pip-Arg-pNA acetate is specific for thrombin and is used to measure antithrombin-heparin cofactor (AT-III). The AT-III assay using H-D-Phe-Pip-Arg-pNA acetate is sensitive, accurate, and easy to perform .

HY-123275A

H-D-Phe-Pip-Arg-pNA hydrochloride 1 Publications Verification S-2238 hydrochloride	Fluorescent Dye	Others
H-D-Phe-Pip-Arg-pNA (S-2238) hydrochloride, a chromogenic substrate, is patterned after the N-terminal portion of the A alpha chain of fibrinogen, which is the natural substrate of thrombin. H-D-Phe-Pip-Arg-pNA hydrochloride is specific for thrombin and is used to measure antithrombin-heparin cofactor (AT-III). The AT-III assay using H-D-Phe-Pip-Arg-pNA hydrochloride is sensitive, accurate, and easy to perform .

HY-P1003

Ac-DEVD-AMC 4 Publications Verification	Fluorescent Dye	Others
Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm .

HY-P3936

Endotoxin Substrate	Fluorescent Dye	Others
Endotoxin Substrate (Boc-LGR-pNA) is a chromogenic substrate can be used in quantitative assays of endotoxin .

HY-P4202

Suc-Ala-Glu-Pro-Phe-pNA Suc-AEPF-pNA	Biochemical Assay Reagents	Others
Suc-Ala-Glu-Pro-Phe-pNA (Suc-AEPF-pNA) is a chromogenic substrate for the peptidyl-prolyl isomerase Pin1. Suc-Ala-Glu-Pro-Phe-pNA is used to evaluate the inhibitory effect of target compounds on Pin1, the catalytic activity of Pin1, and other related assays .

HY-P0019A

Z-Gly-Gly-Arg-AMC acetate Maximum Cited Publications 11 Publications Verification	Fluorescent Dye Thrombin	Others
Z-Gly-Gly-Arg-AMC acetate is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP) (Ex/Em = 390/480 nm) .

HY-P3949

*Fluorescent Substrate* for Glu-Specific Proteases**	Fluorescent Dye	Others
Fluorescent Substrate for Glu-Specific Proteases is a V8 protease-Specific chromogenic substrate .

HY-148073

MeO-Succ-Arg-Pro-Tyr-AMC TFA	Fluorescent Dye Ser/Thr Protease	Others
MeO-Succ-Arg-Pro-Tyr-AMC TFA is a fluorogenic peptide substrate for chymotrypsin-like proteases that detects protease activity . (measured at 460 nm)

HY-137409

Suc-AAA-pNA	Peptides	Others
Suc-AAA-pNA is a hydrolyzable peptide substrate. Suc-AAA-pNA serves as a chromogenic substrate for porcine pancreatic elastase, and undergoes hydrolysis via a virtual transition state with a minor physical step and a dominant chemical step, thereby forming a stable reactant state .

HY-P0021A

D-Lys(Z)-Pro-Arg-pNA diacetate 1 Publications Verification Spectrozyme PCa; Chromozym Pca diacetate	Fluorescent Dye	Others
D-Lys(Z)-Pro-Arg-pNA diacetate is a chromogenic peptide substrate of activated protein C (APC) .

HY-P5278

Z-Gly-Arg-Thiobenzyl ester	Peptides	Others
Z-Gly-Arg-Thiobenzyl ester is a chromogenic substrate for plasmogen-activated serine proteases .

HY-P10094

β-Ala-Gly-Arg-pNA	Fluorescent Dye	Others
β-Ala-Gly-Arg-pNA is a chromogenic substrate of thrombin with pNA a strong absorbance at 405 nm .

HY-P4460

AAA-pNA	Ser/Thr Protease	Cancer
AAA-pNA is a chromogenic substrate of Tripeptidyl-peptidase II. AAA-pNA can be used to test Tripeptidyl-peptidase II activity .

HY-P4518

Z-Val-Gly-Arg-pNA	Ser/Thr Protease	Others
Z-Val-Gly-Arg-pNA is a chromogenic substrate for urokinase. Z-Val-Gly-Arg-pNA can be used for determination of urokinase activity .

HY-P4517

Z-Tyr-Lys-Arg-pNA	Peptides	Others
Z-Tyr-Lys-Arg-pNA is a chromogenic substrate. Z-Tyr-Lys-Arg-pNA can be used to detect the activity of subtilisin-type and yapsin-like proteases .

HY-P4453

Boc-Ala-Ala-Asp-pNA	Caspase	Cancer
Boc-Ala-Ala-Asp-pNA is a chromogenic substrate of granzyme B. Boc-Ala-Ala-Asp-pNA can be used to test functional activity of granzyme B .

HY-P4516

Z-Phe-Leu-Glu-pNA	Peptides	Cancer
Z-Phe-Leu-Glu-pNA is a chromogenic substrate for glutamyl endopeptidases. Z-Phe-Leu-Glu-pNA has been used as a synthetic substrate in the study of proteolytic enzymes, including trypsin treatment, subtilisin and chymotrypsin. Z-Phe-Leu-Glu-pNA plays an important role in hormone disorders such as prostate cancer and breast cancer .

HY-123275C

H-D-Phe-Pip-Arg-pNA dihydrochloride S-2238 dihydrochloride	Fluorescent Dye	Others
H-D-Phe-Pip-Arg-pNA (S-2238) dihydrochloride, a chromogenic substrate, is patterned after the N-terminal portion of the A alpha chain of fibrinogen, which is the natural substrate of thrombin. H-D-Phe-Pip-Arg-pNA dihydrochloride is specific for thrombin and is used to measure antithrombin-heparin cofactor (AT-III). The AT-III assay using H-D-Phe-Pip-Arg-pNA dihydrochloride is sensitive, accurate, and easy to perform .

HY-P4484

Suc-AAPK-pNA	Biochemical Assay Reagents	Others
Suc-AAPK-pNA is a chromogenic substrate for the determination of serine/threonine kinase activity and enzyme kinetic parameters .

HY-P3126

Suc-Ile-Glu(γ-pip)-Gly-Arg-pNA hydrochloride	Factor Xa	Others
Suc-Ile-Glu(γ-pip)-Gly-Arg-pNA hydrochloride is a factor Xa specific chromogenic substrate .

HY-P10145

Ac-LEVD-pNA	Biochemical Assay Reagents Caspase	Others
Ac-LEVD-pNA is a chromogenic substrate for caspase-4 and can be used to detect caspase-4 activity .

HY-P3934

*HIV Protease Substrate* I**	HIV Protease	Infection
HIV Protease Substrate I is a chromogenic substrate of HIV-1 protease. HIV Protease Substrate I has the cleavage site of HIV protease .

HY-123275

H-D-Phe-Pip-Arg-pNA S-2238	Fluorescent Dye	Others
H-D-Phe-Pip-Arg-pNA (S-2238), a chromogenic substrate, is patterned after the N-terminal portion of the A alpha chain of fibrinogen, which is the natural substrate of thrombin. H-D-Phe-Pip-Arg-pNA is specific for thrombin and is used to measure antithrombin-heparin cofactor (AT-III). The AT-III assay using H-D-Phe-Pip-Arg-pNA is sensitive, accurate, and easy to perform .

HY-P1448

Ac-Leu-Arg-AMC	Fluorescent Dye	Neurological Disease
Ac-Leu-Arg-AMC is a fluorogenic peptide substrate.

HY-P3120

Mca-Pro-Leu-Ala-Cys(Mob)-Trp-Ala-Arg-Dap(Dnp)-NH2	MMP Fluorescent Dye	Others
Mca-Pro-Leu-Ala-Cys(Mob)-Trp-Ala-Arg-Dap(Dnp)-NH2 is a fluorogenic substrate for matrix metalloproteinase-14 (MMP-14). Mca-Pro-Leu-Ala-Cys(Mob)-Trp-Ala-Arg-Dap(Dnp)-NH2, upon cleavage by MMP-14, releases 7-methoxycoumarin-4-acetyl (Mca) (Ex/Em = 328/420 nm).

HY-P4039

Ac-EEVVAC-pNA	HCV Protease	Infection
Ac-EEVVAC-pNA is a chromogenic substrate for a continuous spectrophotometric assay of HCV NS3 protease. The sequence EEVVAC is derived from the 5A-5B cleavage junction of the HCV polyprotein .

HY-P4458

Boc-QAR-pNA	Ser/Thr Protease	Cancer
Boc-QAR-pNA is a chromogenic substrate of trypsin. Boc-QAR-pNA can be used to test trypsin activity .

HY-P4482

Suc-AAPE-pNA	Bacterial	Infection
Suc-AAPE-pNA is a chromogenic substrate of glutamyl endopeptidase. Suc-AAPE-pNA can be used to test glutamyl endopeptidase activity .

HY-P4470

Phe-Pro-Ala-pNA	Ser/Thr Protease	Cancer
Phe-Pro-Ala-pNA is a chromogenic substrate of tripeptidyl peptidase. Phe-Pro-Ala-pNA can be used to test tripeptidyl peptidase activity .

HY-P4464

Ala-Phe-Pro-pNA	Ser/Thr Protease	Cancer
Ala-Phe-Pro-pNA is a chromogenic substrate of tripeptidyl peptidase. Ala-Phe-Pro-pNA can be used to test tripeptidyl peptidase activity .

HY-P4464A

Ala-Phe-Pro-pNA TFA	Ser/Thr Protease	Cancer
Ala-Phe-Pro-pNA TFA is a chromogenic substrate of tripeptidyl peptidase. Ala-Phe-Pro-pNA TFA can be used to test tripeptidyl peptidase activity .

HY-P6027A

H-D-CHA-Ala-Arg-pNA diacetate	Fluorescent Dye	Others
H-D-CHA-Ala-Arg-pNA diacetate is the diacetate form of H-D-CHA-Ala-Arg-pNA (HY-P6027). H-D-CHA-Ala-Arg-pNA diacetate is a chromogenic substrate for the amidolytic assay .

HY-P3363

Z-DEVD-AMC	Caspase	Cancer
Z-DEVD-AMC is a selective caspase-3 substrate that can be measured by fluorescence spectrometry. AMC can be used as a fluorescence reference standard for AMC-based enzyme substrates including AMC-based caspase substrates .

HY-P2616

Mca-YVADAP-Lys(Dnp)-OH	Caspase Angiotensin-converting Enzyme (ACE)	Others
Mca-YVADAP-Lys(Dnp)-OH is a fluorogenic substrate for caspase-1 and angiotensin-converting enzyme 2 (ACE2) .

HY-P0022A

pGlu-Pro-Arg-MNA monoacetate 1 Publications Verification	Biochemical Assay Reagents	Others
pGlu-Pro-Arg-MNA monoacetate is a chromogenic substrate.

HY-P11392

Ac-IEAR-pNA	Cholinesterase (ChE) Biochemical Assay Reagents	Others
Ac-IEAR-pNA is the chromogenic substrate for quantifying endotoxin levels. Ac-IEAR-pNA is cleaved by HuBChE to produce p-nitroaniline product .

HY-137798

Chromozym PL	Fluorescent Dye	Others
Chromozym PL is a chromogenic substrate for plasmin, and the enzymatic reaction can be carried out in 0.1mL Tris-HCl buffer (50 mM, pH 7.8). 100 μM Chromozym PL was dissolved and prepared. After adding the hydrolase, the generation of p-nitroaniline (pNA) at 405 nm was continuously observed, and the hydrolysis products were calculated .

HY-P3098

Mca-P-Cha-G-Nva-HA-Dap(DNP)-NH2	MMP	Others
Mca-P-Cha-G-Nva-HA-Dap(DNP)-NH2 is a fluorogenic substrate for matrix metalloproteinase-1 (MMP-1), MMP-3, and MMP-26. Mca-P-Cha-G-Nva-HA-Dap(DNP)-NH2 can be used to quantify MMP-1, MMP-3 and MMP-26 activity .

HY-P4202A

Suc-Ala-Glu-Pro-Phe-pNA TFA Suc-AEPF-pNA TFA	Biochemical Assay Reagents	Others
Suc-Ala-Glu-Pro-Phe-pNA (Suc-AEPF-pNA ) TFA is a chromogenic substrate for the peptidylprolyl isomerase Pin1. Suc-Ala-Glu-Pro-Phe-pNA TFA can be used to evaluate the inhibitory effect of the target compound on Pin1, and catalytic activity of Pin1, etc .

HY-P0022

pGlu-Pro-Arg-MNA	Biochemical Assay Reagents	Others
pGlu-Pro-Arg-MNA is a chromogenic substrate.

HY-P10083

Ac-VQVD-pNA	Fluorescent Dye	Others
Ac-VQVD-pNA is a chromogenic substrate of caspase 3 .

HY-P6027

H-D-CHA-Ala-Arg-pNA	Thrombin	Others
H-D-CHA-Ala-Arg-pNA is a chromogenic substrate for the amidolytic assay .

HY-P4491

Suc-Leu-Leu-Val-Tyr-pNA	Peptides	Others
Suc-Leu-Leu-Val-Tyr-pNA is a chymotrypsin-like chromogenic substrate that can be used to detect chymotrypsin enzymatic activity .

HY-P4475

Sar-Pro-Arg-pNA	Thrombin	Cardiovascular Disease
Sar-Pro-Arg-pNA is a chromogenic substrate of α-thrombin. Sar-Pro-Arg-pNA can be used to test α-thrombin activity .

HY-P10005

D-Val-Gly-Arg-pNA	Ser/Thr Protease Plasminogen/Plasmin	Cardiovascular Disease
D-Val-Gly-Arg-pNA is a chromogenic substrate of TPA (tissue plasminogen activator). D-Val-Gly-Arg-pNA can be used to detect the amidolytic activity of TPA I and TPA II .

HY-P3480

H-Trp-Phe-Tyr-Ser(PO3H2)-Pro-Arg-pNA	Fluorescent Dye	Others
H-Trp-Phe-Tyr-Ser(PO3H2)-Pro-Arg-pNA is a chromogenic substrate for Pin1. Pin1 is an essential and conserved mitotic peptidyl-prolyl isomerase, and can recognize the phosphoserine-proline bonds present in mitotic phosphoproteins .

HY-P0021

D-Lys(Z)-Pro-Arg-pNA Chromozym Pca	Fluorescent Dye	Others
D-Lys(Z)-Pro-Arg-pNA (Chromozym Pca) is a chromogenic peptide substrate of activated protein C (APC) .

Cat. No.	Product Name	Category	Target	Chemical Structure

HY-12591A

D-Luciferin 105+ Cited Publications D-(-)-Luciferin; Firefly luciferin; Beetle Luciferin	Natural Products Animals Other Diseases Disease Research Fields Source Classification	Fluorescent Dye
D-luciferin is the natural substrate of the enzyme luciferase (Luc) that catalyzes the production of the typical yellowgreen light of fireflies. The 560 nm chemiluminescence from this reaction peaks within seconds, with light output that is proportional to luciferase concentration when the substrate luciferin is present in excess. The luciferase (luc) gene is a popular reporter gene for research and agent screening. Chemiluminescent techniques are virtually background-free, making the luc reporter gene ideal for detecting low-level gene expression. As little as 0.02 pg of luciferase can be reliably measured in a standard scintillation counter. In addition to its role as a reporter of gene expression, luciferase is commonly used in an extremely sensitive assay for ATP . We of er the firefly luciferase (HY-P1004A), luciferin free acid (HY-12591A), as well as its water-soluble sodium salts (HY-12591) and potassium salts (HY-12591B) .

HY-N0116

Hematoxylin 10+ Cited Publications Natural Black 1; Haematoxylin	Classification of Application Fields Leguminosae Caesalpinia sappan L. Other Diseases Phenols Polyphenols Plants Disease Research Fields Source Classification	Amyloid-β
Hematoxylin (Natural Black 1), a naturally occurring flavonoid compound derived from Caesalpinia sappan Linn.. Hematoxylin is a nuclear stain in histology and is also a potent Aβ42 fibrillogenesis inhibitor with an IC₅₀ of 1.6 μM.

HY-113245

3-Indolyl-β-D-glucuronide	Alkaloids Classification of Application Fields Other Diseases Endogenous metabolite Disease Research Fields Indole Alkaloids Source Classification	Endogenous Metabolite
3-Indolyl-β-D-glucuronide, a chromogenic substrate for β-D-glucuronidase, employs in the detection and enumeration of E. coli, yielding a blue precipitate upon cleavage. 3-Indolyl-β-D-glucuronide in? patients' plasma act as a new indicator of renal failure .

Cat. No.	Product Name	Chemical Structure

HY-W707394

NOBA-d15
NOBA-d₁₅ is the deuterium labeled NOBA (HY-137799). NOBA is a synthetic chromogenic substrate that can be used to detect the enzyme activity of AplTX-II. NOBA can be used in the research of phospholipid hydrolysis .

Cat. No.	Product Name		Classification

HY-D0995

7-Methyl-6-thioguanosine 2 Publications Verification MESG		Nucleoside Analogs Guanosine
7-Methyl-6-thioguanosine (MESG) is a chromogenic substrate that can be converted to 7-methyl-6-thioguanine in the presence of purine nucleoside phosphorylase and inorganic phosphate. 7-Methyl-6-thioguanosine can be used to quantify inorganic phosphate. 7-Methyl-6-thioguanosine can also be used to determine the activities of purine nucleoside phosphorylase and protein phosphatases .

Inquiry Online

Your information is safe with us. * Required Fields.

MCE Japan Authorized Agent ナミキ商事株式会社コスモ・バイオ株式会社重松貿易株式会社
Salutation			Country or Region *
Applicant Name *			Organization Name *
Department *
Email Address *			Product Name *
Cat. No.			Requested quantity *
Phone Number *
Remarks

Inquiry Online

Inquiry Information

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

chromogenic+substrate

Inhibitors & Agonists

Fluorescent Dyes

Biochemical Assay Reagents

Peptides

NaturalProducts

Isotope-Labeled Compounds

Oligonucleotides

Natural
Products