L-Arginine p-nitroanilide dihydrochloride, 98%
Based on 1 Customer Validation
L-Arginine p-nitroanilide dihydrochloride, 98% (H-Arg-pNA 2HCl, 98%) is a chromogenic substrate for cathepsin H. L-Arginine p-nitroanilide dihydrochloride, 98% can be hydrolyzed to produce a chromogenic product detectable by photometry, which is used to measure the activities of cathepsin H and aminopeptidases.
For research use only. We do not sell to patients.
- Purity : 99.84%
- CAS No.: 40127-11-5
- Formula: C12H20Cl2N6O3
- Molecular Weight:367.23
-
Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
All Cathepsin Isoforms
More
Biological Activity
Description
In Vitro
L-Arginine p-nitroanilide (H-Arg pNA 2HCl) dihydrochloride serves as a functional substrate for cathepsin H activity in the midgut fluid of third-instar western corn rootworm larvae[1].
L-Arginine p-nitroanilide dihydrochloride can derive N-benzoyl-l-arginine (BAPNA), which is hydrolysable by native bovine pancreatic trypsin and its pegylated conjugate and can be applied to biochemical assays. Biochemical assay results indicate that pegylated bovine pancreatic trypsin has stronger autolysis resistance than native bovine pancreatic trypsin at 40 °C[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 40127-11-5
-
Appearance Solid
-
Molecular Weight 367.23
-
Formula C12H20Cl2N6O3
-
Color Off-white to light yellow
-
SMILES
O=C(NC1=CC=C([N+]([O-])=O)C=C1)[C@@H](N)CCCNC(N)=N.Cl.Cl
-
Synonyms
H-Arg-pNA 2HCl, 98%
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
H2O : ≥ 200 mg/mL (544.62 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (270 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
Handling Instructions (2659 KB)
References
[1]. Kaiser-Alexnat R, et al. Protease activities in the midgut of Western corn rootworm (Diabrotica virgifera virgifera LeConte). Journal of invertebrate pathology. 2009 Mar;100(3):169-74. [Content Brief]
[3]. Long WF, et al. Inhibition by heparin-modulated antithrombin III of amidolysis catalysed by m beta-acrosin. The Biochemical journal. 1984 Feb 01;217(3):721-5. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.7231 mL | 13.6154 mL | 27.2309 mL | 68.0772 mL |
| 5 mM | 0.5446 mL | 2.7231 mL | 5.4462 mL | 13.6154 mL | |
| 10 mM | 0.2723 mL | 1.3615 mL | 2.7231 mL | 6.8077 mL | |
| 15 mM | 0.1815 mL | 0.9077 mL | 1.8154 mL | 4.5385 mL | |
| 20 mM | 0.1362 mL | 0.6808 mL | 1.3615 mL | 3.4039 mL | |
| 25 mM | 0.1089 mL | 0.5446 mL | 1.0892 mL | 2.7231 mL | |
| 30 mM | 0.0908 mL | 0.4538 mL | 0.9077 mL | 2.2692 mL | |
| 40 mM | 0.0681 mL | 0.3404 mL | 0.6808 mL | 1.7019 mL | |
| 50 mM | 0.0545 mL | 0.2723 mL | 0.5446 mL | 1.3615 mL | |
| 60 mM | 0.0454 mL | 0.2269 mL | 0.4538 mL | 1.1346 mL | |
| 80 mM | 0.0340 mL | 0.1702 mL | 0.3404 mL | 0.8510 mL | |
| 100 mM | 0.0272 mL | 0.1362 mL | 0.2723 mL | 0.6808 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
L-Arginine p-nitroanilide dihydrochloride, 98% Related Classifications
HY-137895 Related Classifications
Powered by Bioz