Suc-Ala-Glu-Pro-Phe-pNA TFA
Suc-Ala-Glu-Pro-Phe-pNA (Suc-AEPF-pNA ) TFA is a chromogenic substrate for the peptidylprolyl isomerase Pin1. Suc-Ala-Glu-Pro-Phe-pNA TFA can be used to evaluate the inhibitory effect of the target compound on Pin1, and catalytic activity of Pin1, etc.
For research use only. We do not sell to patients.
- Formula: C34H39F3N6O13
- Molecular Weight:796.70
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Suc-Ala-Glu-Pro-Phe-pNA (Suc-AEPF-pNA) TFA functions as a substrate for recombinant human Pin1 in a cell-free protease-coupled PPIase assay, enabling measurement of Pin1 isomerase activity via pNA release[1].
Suc-Ala-Glu-Pro-Phe-pNA (Suc-AEPF-pNA) TFA (600 nM) specifically elutes phage bound to the Pin1 PPIase domain active site, demonstrating it interacts with the domain's active site[2].
Suc-Ala-Glu-Pro-Phe-pNA (Suc-AEPF-pNA) TFA (0.33 ± 0.06 mM) serves as a substrate for GST-tagged Pin1 PPIase activity, with a KM of 0.33 mM and a kCAT of 7.3 s?1[2].
(2.5 mM) acts as a substrate for recombinant human Pin1's PPIase activity, which is progressively inhibited as Pin1 is phosphorylated by PKA over time, with ~85% inhibition of Pin1's ability to isomerize Suc-Ala-Glu-Pro-Phe-pNA observed after 1 h of PKA pre-incubation[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Molecular Weight 796.70
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Formula C34H39F3N6O13
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Synonyms
Suc-AEPF-pNA TFA
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Sequence
{Suc}-Ala-Glu-Pro-Phe-{pNA}
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Sequence Shortening
{Suc}-AEPF-{pNA}
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Hidaka M, et al. Food polyphenols targeting peptidyl prolyl cis/trans isomerase Pin1. Biochem Biophys Res Commun. 2018;499(3):681-687. [Content Brief]
[2]. Duncan KE, et al. Discovery and characterization of a nonphosphorylated cyclic peptide inhibitor of the peptidylprolyl isomerase, Pin1. J Med Chem. 2011;54(11):3854-3865. [Content Brief]
[3]. Kay DF, et al. Dissecting the functional behavior of the differentially phosphorylated prolyl isomerase, Pin1. Protein Sci. 2024;33(9):e5138. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Suc-Ala-Glu-Pro-Phe-pNA TFA Related Classifications
HY-P4202A Related Classifications
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