Suc-Ala-Glu-Pro-Phe-pNA TFA  (Synonyms: Suc-AEPF-pNA TFA)

Suc-Ala-Glu-Pro-Phe-pNA (Suc-AEPF-pNA ) TFA is a chromogenic substrate for the peptidylprolyl isomerase Pin1. Suc-Ala-Glu-Pro-Phe-pNA TFA can be used to evaluate the inhibitory effect of the target compound on Pin1, and catalytic activity of Pin1, etc^[1][2].

Suc-Ala-Glu-Pro-Phe-pNA (Suc-AEPF-pNA) TFA functions as a substrate for recombinant human Pin1 in a cell-free protease-coupled PPIase assay, enabling measurement of Pin1 isomerase activity via pNA release^[1].
Suc-Ala-Glu-Pro-Phe-pNA (Suc-AEPF-pNA) TFA (600 nM) specifically elutes phage bound to the Pin1 PPIase domain active site, demonstrating it interacts with the domain's active site^[2].
Suc-Ala-Glu-Pro-Phe-pNA (Suc-AEPF-pNA) TFA (0.33 ± 0.06 mM) serves as a substrate for GST-tagged Pin1 PPIase activity, with a K_M of 0.33 mM and a k_CAT of 7.3 s^?1[2].
(2.5 mM) acts as a substrate for recombinant human Pin1's PPIase activity, which is progressively inhibited as Pin1 is phosphorylated by PKA over time, with ~85% inhibition of Pin1's ability to isomerize Suc-Ala-Glu-Pro-Phe-pNA observed after 1 h of PKA pre-incubation^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

796.70

C₃₄H₃₉F₃N₆O₁₃

{Suc}-Ala-Glu-Pro-Phe-{pNA}

{Suc}-AEPF-{pNA}

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Hidaka M, et al. Food polyphenols targeting peptidyl prolyl cis/trans isomerase Pin1. Biochem Biophys Res Commun. 2018;499(3):681-687.  [Content Brief]

  [2]. Duncan KE, et al. Discovery and characterization of a nonphosphorylated cyclic peptide inhibitor of the peptidylprolyl isomerase, Pin1. J Med Chem. 2011;54(11):3854-3865.  [Content Brief]

  [3]. Kay DF, et al. Dissecting the functional behavior of the differentially phosphorylated prolyl isomerase, Pin1. Protein Sci. 2024;33(9):e5138.  [Content Brief]