Suc-Ala-Glu-Pro-Phe-pNA
Based on 1 Customer Validation
Suc-Ala-Glu-Pro-Phe-pNA (Suc-AEPF-pNA) is a chromogenic substrate for the peptidyl-prolyl isomerase Pin1. Suc-Ala-Glu-Pro-Phe-pNA is used to evaluate the inhibitory effect of target compounds on Pin1, the catalytic activity of Pin1, and other related assays.
For research use only. We do not sell to patients.
- Purity: 99.45%
- CAS No.: 128802-76-6
- Formula: C32H38N6O11
- Molecular Weight:682.68
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Storage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Suc-Ala-Glu-Pro-Phe-pNA functions as a substrate for recombinant human Pin1 in a cell-free protease-coupled PPIase assay, enabling measurement of Pin1 isomerase activity via pNA release[1].
Suc-Ala-Glu-Pro-Phe-pNA (Suc-AEPF-pNA) (600 nM) specifically elutes phage bound to the Pin1 PPIase domain active site, demonstrating it interacts with the domain's active site[2].
Suc-Ala-Glu-Pro-Phe-pNA (Suc-AEPF-pNA) (0.33 ± 0.06 mM) serves as a substrate for GST-tagged Pin1 PPIase activity, with a KM of 0.33 mM and a kCAT of 7.3 s?1[2].
Suc-Ala-Glu-Pro-Phe-pNA (2.5 mM) acts as a substrate for recombinant human Pin1's PPIase activity, which is progressively inhibited as Pin1 is phosphorylated by PKA over time, with ~85% inhibition of Pin1's ability to isomerize Suc-Ala-Glu-Pro-Phe-pNA observed after 1 h of PKA pre-incubation[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 128802-76-6
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Appearance Solid
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Molecular Weight 682.68
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Formula C32H38N6O11
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Color White to off-white
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Synonyms
Suc-AEPF-pNA
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Sequence
{Suc}-Ala-Glu-Pro-Phe-{pNA}
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Sequence Shortening
{Suc}-AEPF-{pNA}
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 125 mg/mL (183.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Hidaka M, et al. Food polyphenols targeting peptidyl prolyl cis/trans isomerase Pin1. Biochem Biophys Res Commun. 2018;499(3):681-687. [Content Brief]
[2]. Duncan KE, et al. Discovery and characterization of a nonphosphorylated cyclic peptide inhibitor of the peptidylprolyl isomerase, Pin1. J Med Chem. 2011;54(11):3854-3865. [Content Brief]
[3]. Kay DF, et al. Dissecting the functional behavior of the differentially phosphorylated prolyl isomerase, Pin1. Protein Sci. 2024;33(9):e5138. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4648 mL | 7.3241 mL | 14.6482 mL | 36.6204 mL |
| 5 mM | 0.2930 mL | 1.4648 mL | 2.9296 mL | 7.3241 mL | |
| 10 mM | 0.1465 mL | 0.7324 mL | 1.4648 mL | 3.6620 mL | |
| 15 mM | 0.0977 mL | 0.4883 mL | 0.9765 mL | 2.4414 mL | |
| 20 mM | 0.0732 mL | 0.3662 mL | 0.7324 mL | 1.8310 mL | |
| 25 mM | 0.0586 mL | 0.2930 mL | 0.5859 mL | 1.4648 mL | |
| 30 mM | 0.0488 mL | 0.2441 mL | 0.4883 mL | 1.2207 mL | |
| 40 mM | 0.0366 mL | 0.1831 mL | 0.3662 mL | 0.9155 mL | |
| 50 mM | 0.0293 mL | 0.1465 mL | 0.2930 mL | 0.7324 mL | |
| 60 mM | 0.0244 mL | 0.1221 mL | 0.2441 mL | 0.6103 mL | |
| 80 mM | 0.0183 mL | 0.0916 mL | 0.1831 mL | 0.4578 mL | |
| 100 mM | 0.0146 mL | 0.0732 mL | 0.1465 mL | 0.3662 mL |
Suc-Ala-Glu-Pro-Phe-pNA Related Classifications
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