D-Lys(Z)-Pro-Arg-pNA
Based on 1 publication(s) in Google Scholar
D-Lys(Z)-Pro-Arg-pNA (Chromozym Pca) is a chromogenic peptide substrate of activated protein C (APC).
For research use only. We do not sell to patients.
- CAS No.: 108963-69-5
- Formula: C31H43N9O7
- Molecular Weight:653.73
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) D-Lys(Z)-Pro-Arg-pNA
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Biological Activity
Cleavage of Chromogenic Substrates.[1]
APC derivatives (2 nM) and D-Lys(Z)-Pro-Arg-pNA (15-2000 μM) are mixed in 0.1 M NaCl, 0.02 M Tris-HCl (TBS, pH 7.5) containing 2.5 mM Ca2+, 0.1 mg/mL BSA, and 0.1% PEG 8000.
The rate of hydrolysis was measured at 405 nm at room temperature in a Vmax kinetic microplate reader.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 108963-69-5
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Molecular Weight 653.73
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Formula C31H43N9O7
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Synonyms
Chromozym Pca
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Sequence
{d-Lys(Z)}-Pro-Arg-pNA
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Sequence Shortening
{d-Lys(Z)}-PR-pNA
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
H2O
Peptide Solubility and Storage Guidelines:
1. Calculate the length of the peptide.
2. Calculate the overall charge of the entire peptide according to the following table:
| Contents | Assign value | |
| Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 |
| Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 |
| Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution:
| Overall charge of peptide | Details |
| Negative (<0) |
1. Try to dissolve the peptide in water first. 2. If water fails, add NH4OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. |
| Positive (>0) |
1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. |
| Zero (=0) |
1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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