4-Nitrocatechol sulfate dipotassium salt  (Synonyms: Dipotassium nitrocatechol sulfate)

4-Nitrocatechol sulfate potassium salt (Dipotassium nitrocatechol sulfate) is an aromatic sulfate that can serve as a chromogenic substrate for sulfatase. 4-Nitrocatechol sulfate potassium salt can be used to determine the activities of glycosaminoglycan (GAG) degrading enzymes aryl sulfatase B and exonucleosidase^[1][2][3].

Assay of arylsulfatase A^[3]:
1. 0.8 mL of 0.01 M 4-nitrocatechol sulfate in 0.5 M sodium acetate buffer (pH 5.0) containing 0.5 mM Na₄P₂O₇ and 10% (w/v) sodium chloride were mixed with 0.2 mL of enzyme preparation and incubated for 1 hr at 37 °C.
2. The reaction was stopped by adding 0.2 mL of 10% trichloroacetic acid.
3. The mixture was centrifuged for 10 min at 2,000 rpm.
4. 0.2 mL of 2N NaOH was added to the supernatant and the amount of 4-nitrocatechol liberated was estimated by measuring the absorption at 515 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

311.35

C₆H₃K₂NO₇S

14528-64-4

Solid

Light yellow to yellow

O=[N+](C1=CC=C(O[K])C(OS(=O)(O[K])=O)=C1)[O-]

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. ROY AB. The sulphatase of ox liver. I. The complex nature of the enzyme. Biochem J. 1953 Jan;53(1):12-5.  [Content Brief]

  [2]. Kowalewski R, et al. Evaluation of enzymes involved in proteoglycan degradation in the wall of abdominal aortic aneurysms. J Vasc Res. 2006;43(1):95-100.  [Content Brief]

  [3]. Yamato K, et al. Purification of arylsulfatase A from boar testis and its activities toward seminolipid and sulfatide. J Biochem. 1974 Jun;75(6):1241-7.  [Content Brief]