4-Nitrocatechol sulfate dipotassium salt
Based on 1 Customer Validation
4-Nitrocatechol sulfate potassium salt (Dipotassium nitrocatechol sulfate) is an aromatic sulfate that can serve as a chromogenic substrate for sulfatase. 4-Nitrocatechol sulfate potassium salt can be used to determine the activities of glycosaminoglycan (GAG) degrading enzymes aryl sulfatase B and exonucleosidase.
For research use only. We do not sell to patients.
- Purity: 99.78%
- CAS No.: 14528-64-4
- Formula: C6H3K2NO7S
- Molecular Weight:311.35
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Assay of arylsulfatase A[3]:
1. 0.8 mL of 0.01 M 4-nitrocatechol sulfate in 0.5 M sodium acetate buffer (pH 5.0) containing 0.5 mM Na4P2O7 and 10% (w/v) sodium chloride were mixed with 0.2 mL of enzyme preparation and incubated for 1 hr at 37 °C.
2. The reaction was stopped by adding 0.2 mL of 10% trichloroacetic acid.
3. The mixture was centrifuged for 10 min at 2,000 rpm.
4. 0.2 mL of 2N NaOH was added to the supernatant and the amount of 4-nitrocatechol liberated was estimated by measuring the absorption at 515 nm.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 14528-64-4
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Appearance Solid
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Molecular Weight 311.35
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Formula C6H3K2NO7S
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Color Light yellow to yellow
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SMILES
O=[N+](C1=CC=C(O[K])C(OS(=O)(O[K])=O)=C1)[O-]
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Synonyms
Dipotassium nitrocatechol sulfate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 100 mg/mL (321.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (321.18 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. ROY AB. The sulphatase of ox liver. I. The complex nature of the enzyme. Biochem J. 1953 Jan;53(1):12-5. [Content Brief]
[2]. Kowalewski R, et al. Evaluation of enzymes involved in proteoglycan degradation in the wall of abdominal aortic aneurysms. J Vasc Res. 2006;43(1):95-100. [Content Brief]
[3]. Yamato K, et al. Purification of arylsulfatase A from boar testis and its activities toward seminolipid and sulfatide. J Biochem. 1974 Jun;75(6):1241-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 3.2118 mL | 16.0591 mL | 32.1182 mL | 80.2955 mL |
| 5 mM | 0.6424 mL | 3.2118 mL | 6.4236 mL | 16.0591 mL | |
| 10 mM | 0.3212 mL | 1.6059 mL | 3.2118 mL | 8.0295 mL | |
| 15 mM | 0.2141 mL | 1.0706 mL | 2.1412 mL | 5.3530 mL | |
| 20 mM | 0.1606 mL | 0.8030 mL | 1.6059 mL | 4.0148 mL | |
| 25 mM | 0.1285 mL | 0.6424 mL | 1.2847 mL | 3.2118 mL | |
| 30 mM | 0.1071 mL | 0.5353 mL | 1.0706 mL | 2.6765 mL | |
| 40 mM | 0.0803 mL | 0.4015 mL | 0.8030 mL | 2.0074 mL | |
| 50 mM | 0.0642 mL | 0.3212 mL | 0.6424 mL | 1.6059 mL | |
| 60 mM | 0.0535 mL | 0.2677 mL | 0.5353 mL | 1.3383 mL | |
| 80 mM | 0.0401 mL | 0.2007 mL | 0.4015 mL | 1.0037 mL | |
| 100 mM | 0.0321 mL | 0.1606 mL | 0.3212 mL | 0.8030 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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