4-Nitrophenyl palmitate
Based on 1 Customer Validation
4-Nitrophenyl palmitate (4-Nitrophenyl hexadecanoate) is a chromogenic substrate for lipases and esterases. Upon enzymatic hydrolysis, 4-Nitrophenyl palmitate releases p-nitrophenol, which can be quantified by colorimetric detection at 410 nm as a measure of enzymatic activity. 4-Nitrophenyl palmitate is used to characterize the activity of various bacterial and mammalian enzymes, including those from Burkholderia and porcine pancreatic lipase.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 1492-30-4
- Formula: C22H35NO4
- Molecular Weight:377.52
-
Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
4-Nitrophenyl palmitate serves as a substrate for measuring esterolytic activity of porcine pancreatic lipase, Staphylococcus aureus protease, and Burkholderia sp. lipase via colorimetric detection of released p-nitrophenol, with turbidity issues resolved by adding Triton X-100 to the reaction mixture[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 1492-30-4
-
Appearance Solid
-
Molecular Weight 377.52
-
Formula C22H35NO4
-
Color White to off-white
-
SMILES
CCCCCCCCCCCCCCCC(OC1=CC=C([N+]([O-])=O)C=C1)=O
-
Synonyms
4-Nitrophenyl hexadecanoate; p-Nitrophenyl Palmitate; pNpp
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
Chloroform : ≥ 100 mg/mL (264.89 mM)
DMSO : 6.67 mg/mL (17.67 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (269 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / Chloroform | 1 mM | 2.6489 mL | 13.2443 mL | 26.4887 mL | 66.2217 mL |
| 5 mM | 0.5298 mL | 2.6489 mL | 5.2977 mL | 13.2443 mL | |
| 10 mM | 0.2649 mL | 1.3244 mL | 2.6489 mL | 6.6222 mL | |
| 15 mM | 0.1766 mL | 0.8830 mL | 1.7659 mL | 4.4148 mL | |
| Chloroform | 20 mM | 0.1324 mL | 0.6622 mL | 1.3244 mL | 3.3111 mL |
| 25 mM | 0.1060 mL | 0.5298 mL | 1.0595 mL | 2.6489 mL | |
| 30 mM | 0.0883 mL | 0.4415 mL | 0.8830 mL | 2.2074 mL | |
| 40 mM | 0.0662 mL | 0.3311 mL | 0.6622 mL | 1.6555 mL | |
| 50 mM | 0.0530 mL | 0.2649 mL | 0.5298 mL | 1.3244 mL | |
| 60 mM | 0.0441 mL | 0.2207 mL | 0.4415 mL | 1.1037 mL | |
| 80 mM | 0.0331 mL | 0.1656 mL | 0.3311 mL | 0.8278 mL | |
| 100 mM | 0.0265 mL | 0.1324 mL | 0.2649 mL | 0.6622 mL |
Powered by Bioz