p-Nitrophenyl phosphorylcholine
Based on 1 Customer Validation
p-Nitrophenyl phosphorylcholine (4-Nitrophenylphosphorylcholine) is a chromogenic Phospholipase C substrate. Phospholipase C hydrolyzes p-Nitrophenyl phosphorylcholine to release p-nitrophenol, a yellow chromophore. p-Nitrophenyl phosphorylcholine is used to determine the phospholipase C production of Pseudomonas aeruginosa. It also applies to studies related to Pseudomonas aeruginosa infection.
For research use only. We do not sell to patients.
- Purity: 99.82%
- CAS No.: 21064-69-7
- Formula: C11H17N2O6P
- Molecular Weight:304.24
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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PLC |
p-Nitrophenyl phosphorylcholine (10 mM; 1 h) enables the colorimetric assay to detect phospholipase C activity in clinical Pseudomonas aeruginosa strains, with urinary tract isolates and nonmucoid cystic fibrosis lung isolates showing significantly higher mean relative activity (3.06 and 1.67, respectively) than other strain groups relative to the PAO1 standard[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 21064-69-7
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Appearance Solid
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Molecular Weight 304.24
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Formula C11H17N2O6P
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Color White to off-white
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SMILES
O=P(OCC[N+](C)(C)C)([O-])OC1=CC=C([N+]([O-])=O)C=C1
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Synonyms
4-Nitrophenylphosphorylcholine; 4-Nitrophenylphosphorylcholine; O-(4-Nitrophenylphosphoryl)choline
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 25 mg/mL (82.17 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (269 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2869 mL | 16.4344 mL | 32.8688 mL | 82.1720 mL |
| 5 mM | 0.6574 mL | 3.2869 mL | 6.5738 mL | 16.4344 mL | |
| 10 mM | 0.3287 mL | 1.6434 mL | 3.2869 mL | 8.2172 mL | |
| 15 mM | 0.2191 mL | 1.0956 mL | 2.1913 mL | 5.4781 mL | |
| 20 mM | 0.1643 mL | 0.8217 mL | 1.6434 mL | 4.1086 mL | |
| 25 mM | 0.1315 mL | 0.6574 mL | 1.3148 mL | 3.2869 mL | |
| 30 mM | 0.1096 mL | 0.5478 mL | 1.0956 mL | 2.7391 mL | |
| 40 mM | 0.0822 mL | 0.4109 mL | 0.8217 mL | 2.0543 mL | |
| 50 mM | 0.0657 mL | 0.3287 mL | 0.6574 mL | 1.6434 mL | |
| 60 mM | 0.0548 mL | 0.2739 mL | 0.5478 mL | 1.3695 mL | |
| 80 mM | 0.0411 mL | 0.2054 mL | 0.4109 mL | 1.0271 mL |
p-Nitrophenyl phosphorylcholine Related Classifications
HY-100045 Related Classifications
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- p-Nitrophenyl phosphorylcholine
- 21064-69-7
- 4-Nitrophenylphosphorylcholine
- 4-Nitrophenylphosphorylcholine
- O-(4-Nitrophenylphosphoryl)choline
- Phospholipase
- Pseudomonas aeruginosa
- phospholipase C mutants
- phospholipase C
- p-nitrophenol
- nonmucoid cystic fibrosis lung isolates
- clinical strains
- alkaline phosphatase
- PAO1
- urinary tract isolates
- Inhibitor
- inhibitor
- inhibit