Potassium p-nitrophenyl sulfate
Based on 1 Customer Validation
Potassium p-nitrophenyl sulfate (4-Nitophenyl Sulfate potassium) serves as a chromogenic substrate for arylsulfatases. Potassium p-nitrophenyl sulfate releases nitrophenol under the cleavage action of arylsulfatases, and the activity of arylsulfatases can be quantitatively determined via colorimetric detection at 400 nm. Potassium p-nitrophenyl sulfate is commonly used in enzyme inhibition assays and metabolite analog-related studies.
For research use only. We do not sell to patients.
- Purity: 99.89%
- CAS No.: 6217-68-1
- Formula: C6H4KNO6S
- Molecular Weight:257.26
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Potassium p-nitrophenyl sulfate releases nitrophenol under the cleavage action of arylsulfatases, and the activity of arylsulfatases can be quantitatively determined via colorimetric detection at 400 nm[1][2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 6217-68-1
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Appearance Solid
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Molecular Weight 257.26
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Formula C6H4KNO6S
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Color Off-white to yellow
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SMILES
O=[N+]([O-])C1=CC=C(C=C1)OS(=O)(O[K])=O
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Synonyms
4-Nitopheyl Sulfate potassium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
H2O : 25 mg/mL (97.18 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 3.8871 mL | 19.4356 mL | 38.8712 mL | 97.1779 mL |
| 5 mM | 0.7774 mL | 3.8871 mL | 7.7742 mL | 19.4356 mL | |
| 10 mM | 0.3887 mL | 1.9436 mL | 3.8871 mL | 9.7178 mL | |
| 15 mM | 0.2591 mL | 1.2957 mL | 2.5914 mL | 6.4785 mL | |
| 20 mM | 0.1944 mL | 0.9718 mL | 1.9436 mL | 4.8589 mL | |
| 25 mM | 0.1555 mL | 0.7774 mL | 1.5548 mL | 3.8871 mL | |
| 30 mM | 0.1296 mL | 0.6479 mL | 1.2957 mL | 3.2393 mL | |
| 40 mM | 0.0972 mL | 0.4859 mL | 0.9718 mL | 2.4294 mL | |
| 50 mM | 0.0777 mL | 0.3887 mL | 0.7774 mL | 1.9436 mL | |
| 60 mM | 0.0648 mL | 0.3239 mL | 0.6479 mL | 1.6196 mL | |
| 80 mM | 0.0486 mL | 0.2429 mL | 0.4859 mL | 1.2147 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Potassium p-nitrophenyl sulfate Related Classifications
HY-W009454 Related Classifications
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