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Potassium p-nitrophenyl sulfate  (Synonyms: 4-Nitopheyl Sulfate potassium)

Cat. No.: HY-W009454 Purity: 99.89%
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SDS
Handling Instructions Technical Support

Potassium p-nitrophenyl sulfate (4-Nitophenyl Sulfate potassium) serves as a chromogenic substrate for arylsulfatases. Potassium p-nitrophenyl sulfate releases nitrophenol under the cleavage action of arylsulfatases, and the activity of arylsulfatases can be quantitatively determined via colorimetric detection at 400 nm. Potassium p-nitrophenyl sulfate is commonly used in enzyme inhibition assays and metabolite analog-related studies.

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Potassium p-nitrophenyl sulfate

Potassium p-nitrophenyl sulfate Chemical Structure

CAS No. : 6217-68-1

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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Potassium p-nitrophenyl sulfate Related Antibodies

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Description

Potassium p-nitrophenyl sulfate (4-Nitophenyl Sulfate potassium) serves as a chromogenic substrate for arylsulfatases. Potassium p-nitrophenyl sulfate releases nitrophenol under the cleavage action of arylsulfatases, and the activity of arylsulfatases can be quantitatively determined via colorimetric detection at 400 nm. Potassium p-nitrophenyl sulfate is commonly used in enzyme inhibition assays and metabolite analog-related studies[1][2].

In Vitro

Potassium p-nitrophenyl sulfate releases nitrophenol under the cleavage action of arylsulfatases, and the activity of arylsulfatases can be quantitatively determined via colorimetric detection at 400 nm[1][2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Potassium p-nitrophenyl sulfate Related Antibodies
Molecular Weight

257.26

Formula

C6H4KNO6S
CAS No.
Appearance

Solid

Color

Off-white to yellow

SMILES

O=[N+]([O-])C1=CC=C(C=C1)OS(=O)(O[K])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 25 mg/mL (97.18 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8871 mL 19.4356 mL 38.8712 mL
5 mM 0.7774 mL 3.8871 mL 7.7742 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.89%

SDS (419 KB)

COA (250 KB) HNMR (248 KB) RP-HPLC (262 KB) MS (69 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 3.8871 mL 19.4356 mL 38.8712 mL 97.1779 mL
5 mM 0.7774 mL 3.8871 mL 7.7742 mL 19.4356 mL
10 mM 0.3887 mL 1.9436 mL 3.8871 mL 9.7178 mL
15 mM 0.2591 mL 1.2957 mL 2.5914 mL 6.4785 mL
20 mM 0.1944 mL 0.9718 mL 1.9436 mL 4.8589 mL
25 mM 0.1555 mL 0.7774 mL 1.5548 mL 3.8871 mL
30 mM 0.1296 mL 0.6479 mL 1.2957 mL 3.2393 mL
40 mM 0.0972 mL 0.4859 mL 0.9718 mL 2.4294 mL
50 mM 0.0777 mL 0.3887 mL 0.7774 mL 1.9436 mL
60 mM 0.0648 mL 0.3239 mL 0.6479 mL 1.6196 mL
80 mM 0.0486 mL 0.2429 mL 0.4859 mL 1.2147 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Potassium p-nitrophenyl sulfate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Potassium p-nitrophenyl6217-68-14-Nitopheyl SulfateFluorescent Dyechromogenic substratearylsulfatasesInhibitorinhibitorinhibit

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