X-alpha-Gal
Based on 1 Customer Validation
X-alpha-Gal is a chromogenic substrate that is used to screen colonies with high activity of β-galactopyranoside. X-alpha-Gal also detects α-galactosidase activity. X-alpha-Gal can be used in yeast two hybrid screening for MEL1 gene activation. X-alpha-Gal can be used with Matchmaker GAL4-based products to confirm protein interactions.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.87%
- CAS. Nr.: 107021-38-5
- Formel: C14H15BrClNO6
- Molecular Weight:408.63
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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HY-15934
X-GALβ-Galactosidase Substrate -
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4-Nitrophenyl a-D-glucopyranosideα-Glucosidase Substrate -
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4-Methylumbelliferyl β-D-galactopyranosideβ-galactosidase Fluorescent Substrate -
HY-15909
BCIPChromogenic Substrate -
HY-W011183
4-Nitrophenyl-N-acetyl-β-D-glucosaminideNAGase Substrate -
HY-136865
MeOSuc-AAPV-AMCElastase Fluorogenic Substrate -
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4-Nitrophenyl β-D-galactopyranosideMonosaccharide Derivative -
HY-W039938
4-Nitrophenyl β-D-xylopyranosideβ-Xylosidase Substrate
Biologische Aktivität
Beschreibung
Chemical Information
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CAS. Nr. 107021-38-5
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Appearance Solid
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Molecular Weight 408.63
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Formel C14H15BrClNO6
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Color White to light yellow
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SMILES
O[C@@H]1[C@@H](O)[C@@H](O)[C@H](O[C@@H]1OC2=CNC3=C2C(Cl)=C(C=C3)Br)CO
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (244.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Protokoll
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CRISPRi/CRISPRa gene-regulation editing
CRISPRi and CRISPRa use catalytically inactive Cas9, typically SpCas9 D10A/H840A, as an RNA-guided DNA-binding platform that targets genomic loci through sgRNA complementarity and an adjacent PAM without generating Cas9 nuclease-mediated DNA cleavage. CRISPRi represses transcription by recruiting dCas9 alone or dCas9 fused to repressor domains such as KRAB to promoters or transcription start site regions, while CRISPRa activates transcription by recruiting activation domains such as VP64, VPR, or SAM components to promoter-proximal regions. The primary readout is target-gene expression change, commonly measured by RT-qPCR, RNA-seq, reporter fluorescence, or protein-level assays, and the readout reflects transcriptional repression or activation at the targeted endogenous locus.
Reinheit & Dokumentation
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
Verweise
[2]. Rac, A., & Fulgosi, H. (2019). Qualitative and quantitative dataset of TROL protein interaction with C3 and C4 ferredoxin: NADP+ oxidoreductases. Data in brief, 28, 105038. [Content Brief]
[3]. Aho, S., et al., (1997). A novel reporter gene MEL1 for the yeast two-hybrid system. Analytical biochemistry, 253(2), 270–272. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4472 mL | 12.2360 mL | 24.4720 mL | 61.1800 mL |
| 5 mM | 0.4894 mL | 2.4472 mL | 4.8944 mL | 12.2360 mL | |
| 10 mM | 0.2447 mL | 1.2236 mL | 2.4472 mL | 6.1180 mL | |
| 15 mM | 0.1631 mL | 0.8157 mL | 1.6315 mL | 4.0787 mL | |
| 20 mM | 0.1224 mL | 0.6118 mL | 1.2236 mL | 3.0590 mL | |
| 25 mM | 0.0979 mL | 0.4894 mL | 0.9789 mL | 2.4472 mL | |
| 30 mM | 0.0816 mL | 0.4079 mL | 0.8157 mL | 2.0393 mL | |
| 40 mM | 0.0612 mL | 0.3059 mL | 0.6118 mL | 1.5295 mL | |
| 50 mM | 0.0489 mL | 0.2447 mL | 0.4894 mL | 1.2236 mL | |
| 60 mM | 0.0408 mL | 0.2039 mL | 0.4079 mL | 1.0197 mL | |
| 80 mM | 0.0306 mL | 0.1530 mL | 0.3059 mL | 0.7648 mL | |
| 100 mM | 0.0245 mL | 0.1224 mL | 0.2447 mL | 0.6118 mL |
X-alpha-Gal Related Classifications
HY-114522 Related Classifications
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