1. Neuronal Signaling
  2. Amyloid-β
  3. Hematoxylin

Hematoxylin  (Synonyms: Natural Black 1; Haematoxylin)

Cat. No.: HY-N0116 Purity: 98.02%
COA Handling Instructions

Hematoxylin (Natural Black 1), a naturally occurring flavonoid compound derived from Caesalpinia sappan Linn.. Hematoxylin is a nuclear stain in histology and is also a potent Aβ42 fibrillogenesis inhibitor with an IC50 of 1.6 μM.

For research use only. We do not sell to patients.

Hematoxylin Chemical Structure

Hematoxylin Chemical Structure

CAS No. : 517-28-2

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 61 In-stock
Solution
10 mM * 1 mL in DMSO USD 61 In-stock
Solid
100 mg USD 55 In-stock
1 g USD 66 In-stock
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Customer Review

Based on 5 publication(s) in Google Scholar

Top Publications Citing Use of Products
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Description

Hematoxylin (Natural Black 1), a naturally occurring flavonoid compound derived from Caesalpinia sappan Linn.. Hematoxylin is a nuclear stain in histology and is also a potent Aβ42 fibrillogenesis inhibitor with an IC50 of 1.6 μM.

IC50 & Target

IC50: 1.6 µM (Aβ42 fibrillogenesis)[2]

In Vitro

When exposed to air, Hematoxylin is oxidized to reddish brown hematein. When oxidized to its hematein form and combined with a mordant, usually a metal salt, Hematoxylin stains tissue sections a deep blue to black color depending on the staining method. By itself, Hematoxylin is also amphoteric in its hematein form; it is red at acid pH and blue at alkaline pH. Differentiation following Hematoxylin staining removes nonspecific staining[1].
Hematoxylin treatment greatly alleviates Aβ42-induced cytotoxicity in SH-SY5Y cells. Hematoxylin is a potential agent against Aβ fibrillogenesis and cytotoxicity[2].
The Hematoxylin and Eosin (H&E) stained tissue section is the cornerstone of anatomical pathology diagnosis. The H&E procedure stains the nucleus and cytoplasm contrasting colors to readily differentiate cellular components[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
The method of H&E staining[4]:
1. Place the glass slides that hold the paraffin sections in staining racks. Clear the paraffin from the samples in three changes of xylene for 2 min per change.
2. Hydrate the samples as follows.
i. Transfer the slides through three changes of 100% ethanol for 2 min per change.
ii. Transfer to 95% ethanol for 2 min.
iii. Transfer to 70% ethanol for 2 min.
iv. Rinse the slides in running tap water at room temperature for at least 2 min.
3. Stain the samples in Hematoxylin solution for 3 min.
4. Place the slides under running tap water at room temperature for at least 5 min.
5. Stain the samples in working eosin Y solution for 2 min.
6. Dehydrate the samples as follows.
i. Dip the slides in 95% ethanol about 20 times.
ii. Transfer to 95% ethanol for 2 min.
iii. Transfer through two changes of 100% ethanol for 2 min per change.
7. Clear the samples in three changes of xylene for 2 min per change.
8. Place a drop of Permount over the tissue on each slide and add a coverslip. View the slides using a microscope.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

302.28

Formula

C16H14O6

CAS No.
Appearance

Solid

Color

Light brown to brown

SMILES

OC1=CC=C([C@]2([H])[C@](CC3=C2C=C(O)C(O)=C3)(O)CO4)C4=C1O

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (165.41 mM; Need ultrasonic)

H2O : 6.67 mg/mL (22.07 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3082 mL 16.5410 mL 33.0819 mL
5 mM 0.6616 mL 3.3082 mL 6.6164 mL
10 mM 0.3308 mL 1.6541 mL 3.3082 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 4.17 mg/mL (13.80 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

  • 4.

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 98.02%

References
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Hematoxylin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Product Name:
Hematoxylin
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HY-N0116
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