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  4. Fluorescent Substrate for Glu-Specific Proteases

Fluorescent Substrate for Glu-Specific Proteases 

Cat. No.: HY-P3949 Purity: 98.16%
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Fluorescent Substrate for Glu-Specific Proteases is a V8 protease-Specific chromogenic substrate.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Fluorescent Substrate for Glu-Specific Proteases Chemical Structure

Fluorescent Substrate for Glu-Specific Proteases Chemical Structure

CAS No. : 143147-75-5

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1 mg USD 58 In-stock
5 mg USD 145 In-stock
10 mg USD 220 In-stock
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Based on 1 publication(s) in Google Scholar

Fluorescent Substrate for Glu-Specific Proteases Related Antibodies

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Description

Fluorescent Substrate for Glu-Specific Proteases is a V8 protease-Specific chromogenic substrate[1].

In Vitro

V8 protease (0, 0.1, 0.5, 1.0, 5.0, 10, 20 µg/ml) is assayed with a solution of 200 µM of the synthetic substrate (Fluorescent Substrate for Glu-Specific Proteases; ABz-AFAFEVFY(NO2)D-OH) in HEPES buffer pH 7.4 and at room temperature conditions. The fluorescence was read each 1 min for 60 min by fluorescence microplate reader[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Fluorescent Substrate for Glu-Specific Proteases Related Antibodies
Molecular Weight

1272.32

Formula

C63H73N11O18
CAS No.
Appearance

Solid

Color

White to light yellow

Sequence Shortening

Abz-AFAFEVFY(NO2)D
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (78.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.7860 mL 3.9298 mL 7.8597 mL
5 mM 0.1572 mL 0.7860 mL 1.5719 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.7860 mL 3.9298 mL 7.8597 mL 19.6491 mL
5 mM 0.1572 mL 0.7860 mL 1.5719 mL 3.9298 mL
10 mM 0.0786 mL 0.3930 mL 0.7860 mL 1.9649 mL
15 mM 0.0524 mL 0.2620 mL 0.5240 mL 1.3099 mL
20 mM 0.0393 mL 0.1965 mL 0.3930 mL 0.9825 mL
25 mM 0.0314 mL 0.1572 mL 0.3144 mL 0.7860 mL
30 mM 0.0262 mL 0.1310 mL 0.2620 mL 0.6550 mL
40 mM 0.0196 mL 0.0982 mL 0.1965 mL 0.4912 mL
50 mM 0.0157 mL 0.0786 mL 0.1572 mL 0.3930 mL
60 mM 0.0131 mL 0.0655 mL 0.1310 mL 0.3275 mL
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Fluorescent Substrate for Glu-Specific Proteases Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fluorescent Substrate for Glu-Specific Proteases143147-75-5Fluorescent DyeV8 proteasechromogenic substrateInhibitorinhibitorinhibit

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