Fluorescent Substrate for Glu-Specific Proteases
Based on 1 Customer Validation
Fluorescent Substrate for Glu-Specific Proteases is a V8 protease-Specific chromogenic substrate.
For research use only. We do not sell to patients.
- Purity: 98.17%
- CAS No.: 143147-75-5
- Formula: C63H73N11O18
- Molecular Weight:1272.32
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Storage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
V8 protease (0, 0.1, 0.5, 1.0, 5.0, 10, 20 µg/ml) is assayed with a solution of 200 µM of the synthetic substrate (Fluorescent Substrate for Glu-Specific Proteases; ABz-AFAFEVFY(NO2)D-OH) in HEPES buffer pH 7.4 and at room temperature conditions. The fluorescence was read each 1 min for 60 min by fluorescence microplate reader[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 143147-75-5
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Appearance Solid
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Molecular Weight 1272.32
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Formula C63H73N11O18
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Color White to light yellow
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Sequence Shortening
Abz-AFAFEVFY(NO2)D
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 100 mg/mL (78.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.7860 mL | 3.9298 mL | 7.8597 mL | 19.6491 mL |
| 5 mM | 0.1572 mL | 0.7860 mL | 1.5719 mL | 3.9298 mL | |
| 10 mM | 0.0786 mL | 0.3930 mL | 0.7860 mL | 1.9649 mL | |
| 15 mM | 0.0524 mL | 0.2620 mL | 0.5240 mL | 1.3099 mL | |
| 20 mM | 0.0393 mL | 0.1965 mL | 0.3930 mL | 0.9825 mL | |
| 25 mM | 0.0314 mL | 0.1572 mL | 0.3144 mL | 0.7860 mL | |
| 30 mM | 0.0262 mL | 0.1310 mL | 0.2620 mL | 0.6550 mL | |
| 40 mM | 0.0196 mL | 0.0982 mL | 0.1965 mL | 0.4912 mL | |
| 50 mM | 0.0157 mL | 0.0786 mL | 0.1572 mL | 0.3930 mL | |
| 60 mM | 0.0131 mL | 0.0655 mL | 0.1310 mL | 0.3275 mL |
Fluorescent Substrate for Glu-Specific Proteases Related Classifications
HY-P3949 Related Classifications
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