3-Cyano-7-ethoxycoumarin
Based on 1 publication(s) in Google Scholar
3-Cyano-7-ethoxycoumarin is a fluorescent cytochrome P-450 substrate that generates a blue fluorescent product upon enzymatic cleavage. 3-Cyano-7-ethoxycoumarin is used to detect the activity of mixed-function oxidases in isolated rat hepatocytes. 3-Cyano-7-ethoxycoumarin serves as a biological dye and indicator for research.
For research use only. We do not sell to patients.
- Purity: 97.40%
- CAS No.: 117620-77-6
- Formula: C12H9NO3
- Molecular Weight:215.20
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 3-Cyano-7-ethoxycoumarin
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Biological Activity
3-Cyano-7-ethoxycoumarin is metabolized by cytochrome P-450-dependent mixed-function oxidases in isolated rat hepatocytes[1].
3-Cyano-7-ethoxycoumarin exhibits fluorescent properties in neutral pH buffer solutions, with the maximum wavelengths of its excitation and emission peaks being 355 nm and 410 nm, respectively[1].
3-Cyano-7-ethoxycoumarin can be converted into 3-cyano-7-hydroxycoumarin, which exhibits fluorescence properties under neutral pH conditions, with maximum excitation and emission wavelengths of 408 nM and 450 nM, respectively[1].
3-Cyano-7-ethoxycoumarin serves as a fluorescent substrate that undergoes dealkylation by rat CYP1A1, human CYP1A1, and human CYP1A2 to produce a fluorescent product[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 117620-77-6
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Appearance Solid
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Molecular Weight 215.20
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Formula C12H9NO3
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Color Light yellow to brown
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SMILES
N#CC1=CC2=CC=C(OCC)C=C2OC1=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Phytomedicine
Cytochrome P450-mediated herb-drug interaction (HDI) of Polygonum multiflorum Thunb. based on pharmacokinetic studies and in vitro inhibition assays. [Abstract]2023 Apr:112:154710. PMID: 36805481
Solvent & Solubility
DMSO : 12.5 mg/mL (58.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. White IN, et al. A continuous fluorometric assay for cytochrome P-450-dependent mixed function oxidases using 3-cyano-7-ethoxycoumarin. Anal Biochem. 1988;172(2):304-310. [Content Brief]
[2]. Stresser DM, et al. Cytochrome P450 fluorometric substrates: identification of isoform-selective probes for rat CYP2D2 and human CYP3A4. Drug Metab Dispos. 2002;30(7):845-852. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.6468 mL | 23.2342 mL | 46.4684 mL | 116.1710 mL |
| 5 mM | 0.9294 mL | 4.6468 mL | 9.2937 mL | 23.2342 mL | |
| 10 mM | 0.4647 mL | 2.3234 mL | 4.6468 mL | 11.6171 mL | |
| 15 mM | 0.3098 mL | 1.5489 mL | 3.0979 mL | 7.7447 mL | |
| 20 mM | 0.2323 mL | 1.1617 mL | 2.3234 mL | 5.8086 mL | |
| 25 mM | 0.1859 mL | 0.9294 mL | 1.8587 mL | 4.6468 mL | |
| 30 mM | 0.1549 mL | 0.7745 mL | 1.5489 mL | 3.8724 mL | |
| 40 mM | 0.1162 mL | 0.5809 mL | 1.1617 mL | 2.9043 mL | |
| 50 mM | 0.0929 mL | 0.4647 mL | 0.9294 mL | 2.3234 mL |
3-Cyano-7-ethoxycoumarin Related Classifications
HY-D0055 Related Classifications
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