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  4. Coelenterazine

Coelenterazine 

Cat. No.: HY-18743 Purity: 98.94%
Handling Instructions

Coelenterazine is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. Renilla luciferase and substrate coelenterazine has been used as the bioluminescence donor in bioluminescence resonance energy transfer (BRET) to detect protein-protein interactions. Coelenterazine is a superoxide anion-sensitive chemiluminescent probe and it can also be used in chemiluminescent detection of peroxynitrite.

For research use only. We do not sell to patients.

Coelenterazine Chemical Structure

Coelenterazine Chemical Structure

CAS No. : 55779-48-1

Size Price Stock Quantity
500 μg USD 66 In-stock
Estimated Time of Arrival: December 31
5 mg USD 385 In-stock
Estimated Time of Arrival: December 31
10 mg USD 671 In-stock
Estimated Time of Arrival: December 31
25 mg USD 1485 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Protocol

  • Purity & Documentation

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  • Customer Review

Description

Coelenterazine is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. Renilla luciferase and substrate coelenterazine has been used as the bioluminescence donor in bioluminescence resonance energy transfer (BRET) to detect protein-protein interactions. Coelenterazine is a superoxide anion-sensitive chemiluminescent probe and it can also be used in chemiluminescent detection of peroxynitrite[1][2][3].

In Vitro

HCT-8 control cells, transiently expressing Renilla luciferase (RLuc), showed low bioluminescence due to P-glycoprotein–mediated efflux transport of coelenterazine. By comparison, transiently expressing RLuc HCT-8 cells, wherein P-glycoprotein was down-regulated with shRNAi, showed high bioluminescence[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The in vivo growth potential of HCC1806-RR was monitored by injecting animals with coelenterazine (2 mg/kg) i.v. and exposing them to a charged-coupled device (CCD) camera 5 minutes later. Rluc activity was detected as light emitted from the tumor cells and acquired as a pseudo-color image superimposed over a black and white photograph of the animal. All mice demonstrated very high Rluc activity at the primary site with the majority of mice simultaneously showing metastases to inguinal ILNs[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

423.46

Formula

C26H21N3O3

CAS No.
SMILES

O=C1C(CC2=CC=C(O)C=C2)=NC3=C(CC4=CC=CC=C4)NC(C5=CC=C(O)C=C5)=CN31

Structure Classification
Source

marine bioluminescent organisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

Ethanol : 2 mg/mL (4.72 mM; Need ultrasonic; DMSO can inactivate Coelenterazine's activity)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3615 mL 11.8075 mL 23.6150 mL
5 mM --- --- ---
10 mM --- --- ---
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 0.2 mg/mL (0.47 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% EtOH    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 0.2 mg/mL (0.47 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% EtOH    90% Saline

    Solubility: ≥ 0.2 mg/mL (0.47 mM); Clear solution

*All of the co-solvents are available by MCE.
References
Cell Assay
[1]

Cells are plated at a density of 5×104 cells per well into 24-well plates and grown to 80-100% confluency. Just before imaging, media are changed to a colorless solution containing (in mM): 2.7 KCl, 139 NaCl, 8.1 Na2HPO4, 0.7 H2O, 1.5 KH2PO4, 1.8 CaCl2, 1 MgCl2 and 5.5 d-glucose. Cells are preincubated for 15 min in the absence or presence of Pgp modulator, after which Coelenterazine (final concentration of 470 nM) is added directly to the cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice are anesthetized with metofane before tail vein injection of Coelenterazine (4 μg/g) formulated from an ethanol stock diluted in sodium phosphate buffer (50 mM). Bioluminescence imaging is performed on the in vivo imaging system at 2, 6, 8, and 11 min after injection. After imaging, animals are killed by cervical dislocation; tumors are then harvested and weighed[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 98.94%

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Coelenterazine
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HY-18743
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