2. Metabolic Enzyme/Protease
  3. Cytochrome P450
  4. 1-Aminobenzotriazole

1-Aminobenzotriazole  (Synonyms: ABT; 3-Aminobenzotriazole)

Cat. No.: HY-103389 Purity: 99.88%
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1-Aminobenzotriazole is a nonspecific and irreversible inhibitor of cytochrome P450 (P450).

For research use only. We do not sell to patients.

CAS No. : 1614-12-6

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Customer Review

Based on 10 publication(s) in Google Scholar

Other Forms of 1-Aminobenzotriazole:

1-Aminobenzotriazole Related Antibodies

Top Publications Citing Use of Products

    1-Aminobenzotriazole purchased from MedChemExpress. Usage Cited in: Plant Cell. 2024 Dec 5:koae290.  [Abstract]

    Phenotype examined before and after cold treatment (−4 °C, 12 h) of trifoliate orange plants that were pretreated with water (H2O), 200 μM SA, 100 μM 1-Aminobenzotriazole (ABT), or ABT plus 1 mm proline (ABT + Pro). Scale bars = 1 cm. The results demonstrated that ABT (an inhibitor of SA biosynthesis) significantly reduced the endogenous SA content within the plants, leading to a substantial decline in their cold tolerance—specifically, a marked increase in their sensitivity to low temperatures (relative to plants not treated with ABT).

    1-Aminobenzotriazole purchased from MedChemExpress. Usage Cited in: Plant Sci. 2023 Nov:336:111859.  [Abstract]

    (a). Phenotypes of 3-week-old detached rosette leaves of Col and NPR1-GFP under constant dark and 50 μM 1-Aminobenzotriazole treatment for 4 days. Bar= 1 cm. (b). Relative chlorophyll content in (a). The results showed that, under dark treatment conditions, 1-Aminobenzotriazole delayed leaf senescence in the Col and NPR1-GFP lines by inhibiting Salicylic acid (SA).

    1-Aminobenzotriazole purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2022 May 16;13:848957.  [Abstract]

    Astilbin promotes cellular ROS production similar to 1-Aminobenzotriazole (ABT, 0.3-2 μM) (a non-specific inhibitor of CYP). Mean ± SD; n = 3.

    1-Aminobenzotriazole purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2022 May 16;13:848957.  [Abstract]

    Astilbin promotes cellular ROS production similar to 1-Aminobenzotriazole (ABT, 0.3-2 μM) (a non-specific inhibitor of CYP). Mean ± SD; n = 3.

    View All Cytochrome P450 Isoform Specific Products:

    View All Isoforms
    CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A CYP3A4 CYP17A1

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    1-Aminobenzotriazole is a nonspecific and irreversible inhibitor of cytochrome P450 (P450).

    IC50 & Target

    CYP2

     

    CYP3

     

    Cellular Effect
    Cell Line Type Value Description References
    HepG2 IC50
    12 μM
    Compound: ABT
    Inhibition of CYP4Z1 (unknown origin) in human HepG2 cell membranes transduced with lentiviral vector using luciferin-benzyl ether as substrate preincubated with NADPH for 30 mins followed by substrate addition and measured after 10 mins by P450-glo luciferase based luminescence assay
    Inhibition of CYP4Z1 (unknown origin) in human HepG2 cell membranes transduced with lentiviral vector using luciferin-benzyl ether as substrate preincubated with NADPH for 30 mins followed by substrate addition and measured after 10 mins by P450-glo luciferase based luminescence assay
    		[PMID: 32302132]
    HepG2 IC50
    154 μM
    Compound: ABT
    Inhibition of CYP4Z1 (unknown origin) in human HepG2 cell membranes transduced with lentiviral vector using luciferin-benzyl ether as substrate incubated for 5 mins followed by NADPH addition and measured after 20 mins by P450-glo luciferase based luminescence assay
    Inhibition of CYP4Z1 (unknown origin) in human HepG2 cell membranes transduced with lentiviral vector using luciferin-benzyl ether as substrate incubated for 5 mins followed by NADPH addition and measured after 20 mins by P450-glo luciferase based luminescence assay
    		[PMID: 32302132]
    In Vitro

    1-Aminobenzotriazole (ABT) alone significantly increases the expression levels of CYP2B6 in two different hepatocytes (7.3- and 10.8-fold, respectively). Upon co-treatment with 1-Aminobenzotriazole, the induction of CYP2B6 expression by CITCO or rifampin is potentiated: 12.6- and 4.0-fold for CITCO as well as 3.9- and 2.5-fold for rifampin. 1-Aminobenzotriazole has a greater potentiation effect on CITCO than on rifampin. 1-Aminobenzotriazole alone increases the expression levels of CYP3A4 in tow different hepatocytes (by 2.0- and 3.8-fold). Upon co-treatment with 1-Aminobenzotriazole, the effects of CITCO on CYP3A4 expression levels are potentiated by 3.8- and 6.0- fold as compare to cells treated with CITCO alone[1].
    1-Aminobenzotriazole (ABT) (1 mM) shows pronounced (~95%) inhibition of the formation of N-acetylprocainamide compare with the control without 1-Aminobenzotriazole[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    1-Aminobenzotriazole Related Antibodies
    In Vivo

    Oral 1-Aminobenzotriazole (ABT) (100 mg/kg, 2 h predose) decreases the clearance of intravenous procainamide (45%) in rats, accompanied by a decreased N-acetylprocainamide-to-procainamide ratio in urine (0.74 versus 0.21) and plasma (area under the curve ratio 0.59 versus 0.11). The urinary recovery of procainamide increases from 18 to 30%, whereas the recovery of N-acetylprocainamide in urine decreases from 13.3 to 6.5% with 1-Aminobenzotriazole[2]. Pretreatment of rats with 100 mg/kg oral 1-Aminobenzotriazole (ABT) administered 2 hours before a semisolid caloric test meal markedly delays gastric emptying. 1-Aminobenzotriazole also increases stomach weights by 2-fold[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    134.14

    Formula

    C6H6N4
    CAS No.
    Appearance

    Solid

    Color

    White to light brown

    SMILES

    NN1N=NC2=CC=CC=C21
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (745.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 50 mg/mL (372.74 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 7.4549 mL 37.2745 mL 74.5490 mL
    5 mM 1.4910 mL 7.4549 mL 14.9098 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (15.51 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (15.51 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 25 mg/mL (186.37 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.88%

    SDS (393 KB)

    COA (251 KB) HNMR (179 KB) LCMS (346 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    Freshly isolated human hepatocytes are used in this study. Briefly, hepatocytes are placed in serum-free Williams’ E media containing 0.1 μM dexamethasone, 10 μg/mL gentamicin, 15 mM HEPES, 2 mM L-glutamine, and 1% ITS. Cells are incubated for 10 hr at 37°C in an atmosphere containing 5% CO2. After recovery, the hepatocytes are treated with media containing CITCO (100 nM), rifampin (10 μM) or vehicle (ethanol), with or without 1-Aminobenzotriazole (ABT) (1 mM) for 72 hr[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [2]

    Male Sprague-Dawley rats (0.26 to 0.30 kg, n=3 per treatment) receive an oral dose of 1-Aminobenzotriazole (ABT) (100 mg/kg, 2 mL/kg) 2 h before a single intravenous bolus of procainamide (10 mg/kg, 2 mL/kg). The control group receives only the intravenous bolus of procainamide without 1-Aminobenzotriazole pretreatment. The vehicle for both 1-Aminobenzotriazole and procainamide is 10% dimethylacetamide/90% water (v/v). Rats are fed 4 h after dosing, and serial blood samples are collected at 0.03, 0.17, 0.25, 0.5, 1, 2, 4, and 6 h postdose. Blood samples are centrifuged using tubes containing K3-EDTA as the anticoagulant to obtain plasma. Urine samples are also collected over 24 h postdose. Plasma and urine samples are frozen at -20°C until analysis[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 7.4549 mL 37.2745 mL 74.5490 mL 186.3724 mL
    5 mM 1.4910 mL 7.4549 mL 14.9098 mL 37.2745 mL
    10 mM 0.7455 mL 3.7274 mL 7.4549 mL 18.6372 mL
    15 mM 0.4970 mL 2.4850 mL 4.9699 mL 12.4248 mL
    20 mM 0.3727 mL 1.8637 mL 3.7274 mL 9.3186 mL
    25 mM 0.2982 mL 1.4910 mL 2.9820 mL 7.4549 mL
    30 mM 0.2485 mL 1.2425 mL 2.4850 mL 6.2124 mL
    40 mM 0.1864 mL 0.9319 mL 1.8637 mL 4.6593 mL
    50 mM 0.1491 mL 0.7455 mL 1.4910 mL 3.7274 mL
    60 mM 0.1242 mL 0.6212 mL 1.2425 mL 3.1062 mL
    80 mM 0.0932 mL 0.4659 mL 0.9319 mL 2.3297 mL
    100 mM 0.0745 mL 0.3727 mL 0.7455 mL 1.8637 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    1-Aminobenzotriazole Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    1-Aminobenzotriazole1614-12-6ABT 3-AminobenzotriazoleCytochrome P450CYPsInhibitorinhibitorinhibit

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